Table 2 Seven EST-SSR primer pairs suitable for analyzing the DNA polymorphism in Bgt
codes | Primers | Sequences | repeat motif | Annealing temperature (°C) |
|---|---|---|---|---|
1-F | Blu SSR3–1 | TTCGAGGCAAGCTCTTCTCA | (CCGTTC)4 | 56 |
1-R | Blu SSR3–2 | TTTCGGCAGGCAAGTTTATT | ||
2-F 2-R | Blu SSR18–1 | GGGTAACGATTGGTTAGGTGCT | (ATCACC)3 | 56 |
Blu SSR18–2 | AGGTGGTGGTAAAGGGGATGAT | |||
3-F 3-R | Blu SSR29–1 | GGAGGATCGGTAGCAGTG | (GCA)5 | 56 |
Blu SSR29–2 | GCGGCGGTAGCTTCTTTT | |||
4-F | Blu SSR32–1 | GGGGAGGTATAGGTGAGG | (TCT)6 | 52 |
4-R | Blu SSR32–2 | GAGCGTTTGCTGTTCTGT | ||
5-F | Blu SSR35–1 | AGACTCACAGCAGAGCAAA | (CTTCAA)3 | 52 |
5-R | Blu SSR35–2 | GCAGATCCATGATCTTCGT | ||
6-F | Blu SSR41–1 | ATCCATTGTAGTTAGGAGCCA | (AC)6 | 54 |
6-R | Blu SSR41–2 | ATGACCTGATTGATTTATCCC | ||
7-F | Blu SSR44–1 | TGAGGATTTAGATGATATGGA | (AGA)5 | 52 |
7-R | Blu SSR44–2 | GATCTTAAATTATTTTGACCG |