Fig. 9

Spatial localization of different neurogenic homologs in the anterior neuroectoderm. Confocal images of double FISH are shown as anterior a, c, e and g and ventral (anterior to the left) views of the brain b, d, f and h. The genes used in double FISH are listed in the figure panels. a–bCt-ngn (red) expression overlaps a subset of the Ct-soxB1⁺ cells (cyan) in the head. c–dCt-ngn (cyan) and Ct-ash1 (red) expression overlaps in a few cells in the head. e–fCt-neuroD (red) expression overlaps Ct-ngn (cyan) only in the intermediate brain. g–h No overlap detected between Ct-ash1 and Ct-neuroD. Closed arrowheads indicate co-expression of two neurogenic homologs while open arrowheads indicate non-overlapping expression of neurogenic homologs in respective panels. Panels b''', d''', f''', h''' are diagrams representing co-expression of respective genes (gray) associated with figure panels b, d, f and h. The orange dotted lines indicate the basal edge of the brain while the white dashed lines indicate the apical boundary of the brain. The different developmental stages investigated are indicated at the lower left corner of each figure panel. Ant, anterior. Scale bar: 25 μm