Table 3 Genetic diversity indices of Heleobia ascotanensis populations determined using 10 microsatellite loci. The number of samples (N), number of alleles (A), number of deprived alleles (A_P), average allelic richness (A_R), percentage of polymorphic loci (% _PL), observed heterozygosity (H_O), expected heterozygosity (H_E), fixation index (F) and internal relatedness (IR) are shown. For % _PL, H_O, H_E, F and IR, the population mean of the parameter and its standard error are shown

Spring 1

30

53

1

2.20

100

0.52 ± 0.08

0.54 ± 0.06

0.01 ± 0.11

0.20 ± 0.04

Spring 2

30

40

0

2.02

100

0.50 ± 0.09

0.48 ± 0.06

−0.04 ± 0.12

0.21 ± 0.04

Spring 3

30

55

0

2.16

100

0.48 ± 0.07

0.53 ± 0.06

0.08 ± 0.10

0.29 ± 0.05

Spring 4

30

43

4

1.99

80

0.26 ± 0.08

0.43 ± 0.09

0.46 ± 0.12

0.47 ± 0.06

Spring 5

30

43

1

2.07

100

0.43 ± 0.08

0.50 ± 0.06

0.12 ± 0.13

0.27 ± 0.07

Spring 6

30

44

3

2.09

100

0.35 ± 0.07

0.48 ± 0.08

0.26 ± 0.10

0.42 ± 0.07

Spring 7

30

52

0

2.32

100

0.46 ± 0.07

0.59 ± 0.04

0.21 ± 0.10

0.35 ± 0.05

Spring 8

30

50

5

2.18

100

0.38 ± 0.08

0.53 ± 0.07

0.26 ± 0.12

0.39 ± 0.05

Spring 9

30

57

0

2.33

100

0.48 ± 0.06

0.59 ± 0.04

0.19 ± 0.10

0.27 ± 0.05

Spring 10

30

60

0

2.40

100

0.64 ± 0.07

0.62 ± 0.04

− 0.02 ± 0.09

0.03 ± 0.04

Spring 11

30

53

2

2.23

90

0.41 ± 0.09

0.53 ± 0.08

0.20 ± 0.13

0.32 ± 0.04

Spring 12

30

61

3

2.36

100

0.60 ± 0.06

0.62 ± 0.04

−0.01 ± 0.12

0.09 ± 0.04

Spring 13

30

69

5

2.25

100

0.60 ± 0.09

0.54 ± 0.08

−0.10 ± 0.10

0.15 ± 0.03

Total

390

124

Mean ± SE

97.7 ± 1.7

0.47 ± 0.02

0.54 ± 0.02

0.12 ± 0.03

0.27 ± 0.01