The transcription factor Krüppel homolog 1is linked to hormone mediated social organization in bees
© Shpigler et al; licensee BioMed Central Ltd. 2010
Received: 9 December 2009
Accepted: 30 April 2010
Published: 30 April 2010
Regulation of worker behavior by dominant queens or workers is a hallmark of insect societies, but the underlying molecular mechanisms and their evolutionary conservation are not well understood. Honey bee and bumble bee colonies consist of a single reproductive queen and facultatively sterile workers. The queens' influences on the workers are mediated largely via inhibition of juvenile hormone titers, which affect division of labor in honey bees and worker reproduction in bumble bees. Studies in honey bees identified a transcription factor, Krüppel-homolog 1 (Kr-h1), whose expression in worker brains is significantly downregulated in the presence of a queen or queen pheromone and higher in forager bees, making this gene an ideal candidate for examining the evolutionary conservation of socially regulated pathways in Hymenoptera.
In contrast to honey bees, bumble bees foragers do not have higher Kr-h1 levels relative to nurses: in one of three colonies levels were similar in nurses and foragers, and in two colonies levels were higher in nurses. Similarly to honey bees, brain Kr-h1 levels were significantly downregulated in the presence versus absence of a queen. Furthermore, in small queenless groups, Kr-h1 levels were downregulated in subordinate workers with undeveloped ovaries relative to dominant individuals with active ovaries. Brain Kr-h1 levels were upregulated by juvenile hormone treatment relative to a vehicle control. Finally, phylogenetic analysis indicates that KR-H1 orthologs are presence across insect orders. Though this protein is highly conserved between honey bees and bumble bees, there are significant differences between orthologs of insects from different orders.
Our results suggest that Kr-h1 is associated with juvenile hormone mediated regulation of reproduction in bumble bees. The expression of this transcription factor is inhibited by the queen and associated with endocrine mediated regulation of social organization in two species of bees. Thus, KR-H1 may transcriptionally regulate a conserved genetic module that is part of a pathway that has been co-opted to function in social behavior, and adjusts the behavior of workers to their social environmental context.
With recent advances in genomics, it is becoming increasingly feasible to elucidate the molecular mechanisms underlying complex behavioral traits in ecologically relevant contexts. One of the key challenges is to determine the level of conservation of genes and genetic pathways involved in producing or regulating similar adaptive behaviors in evolutionarily distinct lineages (reviewed in ). Social insects provide an excellent model system for these studies, because sociality has evolved independently multiple times . One of the hallmarks of insect societies is a reproductive division of labor, in which reproduction is strongly biased toward one or a handful of individuals in the colony (queens) whereas most other colony members (workers) are engaged in very little reproduction or remain sterile. A second important element is a division of labor among the sterile workers, in which workers specialize in various activities such as brood care or foraging (reviewed in [2, 3]). In most insect societies, the interactions between the queen(s) and workers are critical for regulating these divisions of labor. In simple societies, direct contact with the queen and behavioral aggression is often important for inhibiting worker reproduction, while in larger and more evolutionarily derived societies, chemical communication via pheromones is the principle means for advertising the queen's presence [2–5].
Socially regulated behavioral pathways, hereafter referred to as "social pathways", presumably link social signals from the queen and other workers with alterations in worker physiology, which in turn lead to changes in behavioral state (e.g. worker task) that are integrated into an emerging colony level function. These social pathways need to include sensory and integrative systems that ensure that the individual is tuned to the relevant social signals, as well as neuroendocrine signaling systems that integrate the molecular and physiological processes needed for producing the appropriate social behavior. One of the important challenges in sociobiology is to characterize these pathways and compare their structure and function in species showing different levels of social organization. Previous studies in behavioral genomics have focused on genes that detect a particular signal, such as a pheromone, or genes that are involved in the expression of a particular behavior, such as foraging (reviewed in ). While there is ample evidence that the social environment can have significant effects on behavior, physiology, and brain gene expression in a variety of species, genes involved in the intervening "social pathways" have been characterized in only a few species (reviewed in ). For example, the serotonergic system is modulated in crustaceans according to dominance rank, while somatostatin and gonadotropin releasing hormone levels are associated with dominance rank in male cichlid fish [7–10]. In social insects, allelic differences at the Gp-9 locus in fire ants are associated with differences in colony structure, but the number of linked genes in this locus and their molecular functions are not known .
Honey bees and bumble bees are excellent species for comparative studies of this kind, since there are both common elements and important differences in their social organization [2, 3]. Honey bees are highly eusocial insects living in perennial colonies containing several tens of thousands of worker bees. The queen signals her presence by means of a complex set of pheromones that regulate the function and reproduction of the colony . The division of labor among workers is related to age, and is regulated in part by queen pheromones . Bumble bees such as Bombus terrestris are considered "primitively eusocial" because there are no overt morphological differences between the queen and the workers, and the division of labor is less structured . Colonies are founded by a single queen in the spring, and reach a maximal size of only a few hundred workers. Direct contact with the queen is required to inhibit worker reproduction, though this typically does not seem to require aggressive interactions [14, 15]. There is evidence which supports the hypothesis that pheromonal queen signals are involved in the regulation of worker reproduction, but the relative importance of chemical and behavioral signals has not been resolved [16–18]. Worker division of labor between brood care and foraging is based largely on body size rather than on age [2, 3].
Neuroendocrine analyses suggest that there are similarities between the social regulation of reproduction in bumble bees and division of labor in honey bees, specifically concerning the role of juvenile hormone . In honey bees, exposure to queen pheromone decreases circulating levels of juvenile hormone (JH), and slows the transition from nursing to foraging [13, 20]. Forager bees have higher circulating levels of JH, and treatment with JH or JH analogues accelerates the transition to foraging, while JH depletion delays the onset of foraging [21, 22]. In bumble bees, queen presence or body extracts also reduces JH levels. High JH levels are associated with worker ovary activation and reproduction, and high dominance rank in queenless workers [17, 23–25]. JH treatment of workers causes a dose-dependent increase in oocyte length, even in the presence of an inhibitory queen, but does not affect worker task (division of labor) [25–28]. Thus, in bumble bees as in most insects, JH levels are associated with ovary activation and reproduction, while in honey bees and some ant species, juvenile hormone levels are not higher in reproductive queens or workers (for a recent review see ). Thus, this hormonal factor is associated with different behavioral outcomes, but in both cases JH levels are regulated by queen presence. Given that stimuli produced by the queen lead to similar changes in worker physiology but different types of behavioral outcomes, it is possible that the first portions of the "social pathway" are conserved in honey bees and bumble bees, but diverge in terms of the downstream pathways regulating behavior.
Previous studies in honey bees identified a transcription factor, Krüppel homolog 1 (Kr-h1), whose expression in the brains of workers is consistently downregulated by exposure to the queen or queen pheromone [30–32]. As expected, since queen pheromone slows the transition from nursing to foraging, expression of Kr-h1 is also high in foragers compared to nurse bees . Thus, Kr-h1 expression levels may be associated with detection of queen presence, the transition from nursing to foraging, or some aspect of the foraging behavioral state, such as high levels of JH. Furthermore, as a transcription factor, Kr-h1 regulates expression of other genes, and thus may be a key component of signaling pathways linking social environment with behavioral and physiological state.
Here, we tested whether the association of Kr-h1 with a social pathway linking the processing of queen signals with JH regulated behavior is common to social bee species other than the honey bee. Using bumble bees enabled us to uncouple the molecular pathways associated with social inhibition (similar in the honey bee and the bumble bee) from those regulating behavior (distinct in the two species). For example, if Kr-h1 is linked to foraging, then expression should be higher in foragers than nurses in bumble bees as it is in honey bees. If Kr-h1 is associated with queen presence/social inhibition, then expression levels in Bombus should be similar in nurses and foragers, lower in queenright vs queenless workers, and lower in subordinate relative to dominant workers. Furthermore, since JH is linked with foraging behavior in honey bees but with reproductive behavior and dominance rank in bumble bees, these studies will allow us to determine if Kr-h1 expression is correlated with JH levels in both species, regardless of the behavioral state. Finally, we ascertained whether KR-H1 was present and conserved across insect orders. Sequence differences in transcription factors may alter the timing, location, or types of genes regulated by these proteins, and may be an important factor contributing to behavioral or phenotypic evolutionary change .
The influence of queen presence on brain Kr-h1levels in workers
In the second experiment, queenless workers were obtained from orphan colonies, with similar results as above. The workers from the two queenless colonies had approximately twice the amount of brain Kr-h1 RNA compared to the workers from queenright colonies (Fig. 1B; one way ANOVA, P < 0.001; LSD post hoc test, P < 0.001). The queenless and queenright workers were of similar age (4-day-old) and size (queenless colony A, mean ± SE marginal cell size = 2.88 ± 0.12; queenless colony B, 2.94 ± 0.06; queenright colony C, 2.77 ± 0.13; queenright colony D, 2.90 ± 0.03; one-way ANOVA, F = 0.55, P = 0.65). These experiments indicate that the brain expression of Kr-h1 is reduced in the presence of a vital egg-laying queen.
The influence of worker task on brain Kr-h1levels
The influence of dominance rank on Kr-h1levels in groups of queenless workers
The influence of juvenile hormone on ovarian development and brain Kr-h1levels in queenright workers
The influence of topical juvenile hormone (JH-III) treatment on ovarian development in queenright workers.
Kruskal Wallis test
Length of marginal cell (mm)
2.72 ± 0.15 (5)
2.68 ± 0.16 (6)
2.68 ± 0.11(4)
Length of terminal oocyte (mm)
1.90 ± 0.41(5)
1.27 ± 0.23 (5)
0.93 ± 0.09 (4)
Length of marginal cell (mm)
2.74 ± 0.06 (8)
2.74 ± 0.06 (8)
2.89 ± 0.08 (7)
Length of terminal oocyte (mm)
2.41 ± 0.27 (8)
1.08 ± 0.15 (8)
1.20 ± 0.18 (7)
The influence of topical juvenile hormone treatment on brain Kr-h1 levels.
(0.21, 0.18, 10)
(0.12, 0.11, 10)
(0.28, 0.24, 10)
P = 0.018
(0.08, 0.08, 9)
(0.12, 0.11, 8)
(0.09, 0.09, 9)
P = 0.001
Analysis of KR-H1 orthologs across insect orders
There is a high degree of conservation between the predicted B. terrestris KR-H1 protein and the A. mellifera protein, with 97% identity across the whole protein without gaps removed. Interestingly, the sequence of the ortholog from Nasonia vitripennis, a solitary wasp and the only other hymenopteran ortholog currently available, is highly divergent from the two bee species in the C-terminal region. Data from other species will be necessary to determine if this sequence divergence is related to differences in evolutionary lineage or associated with differences in life history or behavior.
While the zinc finger regions are conserved among the insect KR-H1 orthologs, there are important variations in the arrangement of these zinc fingers between the lineages, particularly between dipteran and non-dipteran species, which may have consequences on KR-H1 function. Most importantly, the coding region between zinc finger 1 (Z1) and Z2 contains an insertion-deletion (indel) that varies with taxon. In the dipeteran lineage, the indel ranges from 34-65 aa, and thus is much longer than in other taxa, where the length varies from 9-14 aa. The other zinc fingers are universally separated by 7 residues. In addition to the zinc finger region, there is a conserved region at the C-terminus of KR-H1 orthologs, characterized by LP(L/P)RKR which is separated by a variable indel from RX2SVIX2A at the extreme C-terminus. In the dipteran lineages, the indel ranges from 28 to 60 aa, and thus is much longer than in other taxa where the length varies from 14 to 19 aa.
Regulation of worker behavior and physiology by dominant queens and workers is a hallmark of insect society. In honey bees and bumble bees, this regulation is largely mediated by inhibition of JH levels in workers, which results in inhibition of behavioral maturation in honey bees and reproductive development in bumble bees [13, 19, 23]. These observations support a model in which social stimuli inhibit similar JH-mediated social pathways in both species, but these pathways ultimately regulate different behavioral programs. Together, the studies in honey bees and bumble bees suggest that Kr-h1 is a part of a conserved genetic module which was co-opted to adjust the social behavior of workers to their social environmental context.
In the bumble bee, brain Kr-h1 levels are associated with dominance and reproductive status. Kr-h1 levels are high in the brains of dominant queenless bees, which are characterized by rapid oogenesis and high JH titers. Brain Kr-h1 levels are low in queenright workers and subordinate queenless workers in which oogenesis is inhibited by the presence of a dominant queen or worker [23–25, 34, 38]. In contrast to honey bees, high Kr-h1 levels were not associated with foraging behavior. In fact, in two of the colonies Kr-h1 levels were higher in nurses than in foragers, which is consistent with the general association between high Kr-h1 levels and ovarian activity: oogenesis is typically more rapid in nurses than in foragers of similar age and genotype [34, 28, 39]. However, in both of these groups ovarian development is slower than in queenless workers (see above) which may account for the overall low ovarian activation measured for 7-day-old bees from colonies B and C. The association between Kr-h1 expression, JH, and ovarian activity is further exemplified by the evidence that JH treatment caused an increase in both Kr-h1 expression and ovarian development.
In honey bees, the regulation of Kr-h1 is different. Brain Kr-h1 levels were not significantly different between workers with activated or inactivated ovaries, and Kr-h1 levels were not regulated by mating or reproductive status in queens [40, 41]. While brain Kr-h1 levels are always higher in foragers relative to bees involved in in-hive activities, Kr-h1 levels were not significantly different between foragers and foragers that reverted to performing nursing behavior [31, 42]. Similarly, there is increased neural branching in the mushroom bodies which develops when the bee becomes a forager but does not change when she reverts to brood care . JH treatment affected this processes in some studies, but neuronal branching did occur in bees with no circulating JH, suggesting that JH may affect the pace of mushroom body development, but not its overall occurrence . In this regard, it is interesting to note that there is some evidence that Kr-h1 may play a role in modulating neuromorphology in Drosophila, and expression levels of Kr-h1 are specifically regulated in the mushroom bodies of honey bee workers [32, 45]. These recent studies with honey bees lend credence to the premise that Kr-h1 is more strongly associated with the detection and processing of social stimuli than specifically with foraging behavior or reproduction.
The correlation between JH titers and brain Kr-h1 levels under diverse social conditions in both honey bees and bumble bees suggest that Kr-h1 and JH are involved in the same pathway (or network) that is influenced by signals from the queen and other workers. In the bumble bee B. terrestris, JH treatment caused a significant increase in brain Kr-h1 transcript levels relative to a vehicle treatment, suggesting that JH acts upstream of Kr-h1. This model is consistent with findings in D. melanogaster and Tribolium casteneum, in which Kr-h1 expression is increased by treatment with JH analogs during prepupal and pupal stages [46–48]. In honey bees, it is more difficult to precisely determine the relative position of JH and Kr-h1 in the signaling pathway, and the relationship between these two factors may be more complex. Treatment with the juvenile hormone analog methoprene increased brain Kr-h1 levels in queenright bees . By contrast, in queenless groups of bees, neither methoprene nor JH III treatment raised Kr-h1 levels above those found in control bees (Fussnecker and Grozinger, unpublished observations), and Kr-h1 levels are not always high when JH levels are high . One interpretation for this apparent discrepancy between the treatment effect in queenright and queenless conditions is that JH does influence Kr-h1 expression, but under queenless conditions transcript levels are already high and cannot be increased further by treatment with JH or its analogs. Alternatively, Kr-h1 expression may be regulated by additional factors that mask the influence of JH. The influence of JH treatment on queenless workers has not yet been tested for bumble bees, and thus the relationship between Kr-h1 levels and JH titers in bumble bees might also be affected by social conditions.
Transcription factors like Kr-h1 play an important role in the evolution of physiological, morphological, and behavioral traits . Changes in the amino acid sequence - including the addition or deletion of small protein-binding motifs or alterations in the arrangement of DNA-binding domains - could modulate the function of transcription factors, and the modular architecture of these proteins suggest these changes can occur without large pleiotropic consequences. This sequence variation could alter the types of promoter regions that transcription factors bind to, or modify protein-protein interactions, leading to differences in the signaling pathways that regulate transcription factors. Kr-h1 plays an important role during development and metamorphosis, particularly in development of the central and peripheral nervous system in D. melanogaster and Tribolium castanium [45–49]. However, its function in Hymenoptera at some level may be quite divergent, particularly given the significant sequence differences between dipteran and non-dipteran KR-H1 orthologs. Furthermore, the molecular function of Kr-h1 in adult insects remains to be determined. It is interesting to note that while the B. terrestris and A. mellifera orthologs are nearly identical there are large differences in the C-terminal region of the predicted Kr-h1 ortholog from N. vitripennis, a solitary parasitic wasp. Further comparative genomic studies and functional assays will be necessary to determine if Kr-h1's function truly diverges between social and nonsocial Hymenoptera, and between different insect orders, or if the sequence differences are simply due to the accumulation of mutations during evolution in different lineages.
Our results suggest that Kr-h1 is associated with JH mediated regulation of reproduction in bumble bees. In contrast, in honey bees Kr-h1 is associated with worker division of labor. In both bee species however, the expression of Kr-h1 in the brain is inhibited by the queen and associated with juvenile hormone mediated regulation of social organization. The comparative studies with honey bees and bumble bees suggest that Kr-h1 is part of a conserved pathway linking the perception of social signals from the queen and other workers to JH regulated changes in social behavior. Since the KR-H1 orthologs in honey bees and bumble bees are highly conserved, they may transcriptionally regulate similar genetic modules. However, we hypothesize that downstream of Kr-h1, the pathway may diverge to regulate functions associated with worker task in honey bees and reproduction in bumble bees. Additional studies on the function of Kr-h1 and the genes it regulates may allow a better understanding of the evolution of queen control over worker behavior in social insects.
There is increasing evidence that the genetic pathways underlying developmental, physiological, or behavioral traits are conserved across species, and modifications in the coordination or expression of these pathways can have profound effects and produce distinct phenotypes (reviewed in [50, 51]). Comparisons of honey bees and bumble bees allow us to examine the genetic conservation of socially responsive behavioral pathways, and to elucidate the interplay of specific genes and physiological pathways, such as JH. JH is a principle insect endocrine signal that appears to have a somewhat variable function in social insects. It retains its "classical" gonadotrophic function in some species such as the bumble bee B. terrestris, while in other species such as the honey bee A. mellifera it is instead implicated in the regulation of age-related division of labor [29, 52–54]. Given of the association of social organization, JH levels, and Kr-h1 expression, additional studies on the function of Kr-h1 and the genes it regulates in bees and other insect species will allow us to further characterize this pivotal "social pathway", and ultimately shed light on the evolution of complex insect societies and their enigmatic endocrine regulation.
General Bumble Bee Rearing
We obtained colonies of Bombus terrestris from Polyam Pollination Services, Yad-Mordechai, Israel. Each colony contained a queen, 5-10 workers (about 3 - 7 days after the emergence of the first worker), and brood at different stages of development. Colonies were housed in wooden nesting boxes (30 × 23 × 20 cm) with Plexiglas covers in an environmental chamber (28 ± 1°C; ~50% RH) in constant darkness at the bee research facility at the Edmond J. Safra campus of the Hebrew University of Jerusalem, Givat Ram, Jerusalem. Commercial sugar syrup and fresh pollen (mixed with sugar syrup) were provided ad libitum. All observations and treatments were made under dim red light that the bees cannot see. As an index for the degree of ovarian activity, we measured the length of the largest terminal oocyte [24, 26, 34]. As an index for body size, we measured the length of the front wing margin cell, which is highly correlated with wing length and other indices for body size (e.g., ).
Identification of B. terrestris Kr-h1
Total RNA was extracted from the heads of individual bumble bees using a PicoPure kit (Molecular Devices, Sunnyvale, CA). cDNA was synthesized using 100 ng of total RNA with ArrayScript Reverse Transcriptase (Ambion, Austin, TX). PCR primers designed to A. mellifera Kr-h1 (see Additional file 3 Table S1) were used in a standard PCR reaction mix which also included 5% DMSO in a touchdown PCR reaction. PCR products were separated on a 1% agarose gel. Each primer pair produced a single dominant band. Gel bands were cut for DNA purification (QIAquick gel extraction kit, Qiagen, Valencia, CA). DNA quantity and quality was assayed with a ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington DE). Sequencing was performed with the PCR primers at the NCSU Genome Research Laboratory using the BigDye Terminator sequencing protocol (Applied Biosystems, Foster City, CA). This procedure was repeated on a second B. terrestris sample, as well as an A. mellifera sample from the same apiary at HUJI, to ensure that there was no cross-species contamination and the B. terrestris and A. mellifera sequences were distinct. The B. terrestris Kr-h1 sequence is available in GenBank (GQ903677).
Quantitative real-time PCR (qRT-PCR) analysis of brain gene expression
Bees were collected by flash freezing in liquid nitrogen and immediately transferred to dry ice. Heads were separated and stored at -80°C until RNA analysis. Heads were partially lyophilized to facilitate dissection of brains. The total RNA from the dissected tissue was extracted using an RNAeasy kit (Qiagen, for analyses carried out in NCSU) or Invisorb Spin Tissue RNA Mini Kit (Invitek GmbH, Berlin, Germany, for analyses carried out in HUJI), and quantified via a ND-1000 Spectrophotometer (NanoDrop Technologies). cDNA was synthesized from 250 ng of RNA. To estimate the level of gene expression we used an ABI 7900 (NCSU) or an ABI7000 (HUJI) sequence detector and a SYBR green detection protocol (Applied Biosystems). Elongation Factor 1 a (EF1a) was used as a control housekeeping gene (for more details see [32, 56]). Three technical replicates were performed for each sample/primer set. Quantification was based on the number of PCR cycles (Ct) required to cross a threshold of fluorescence intensity, using the 2-ΔCt technique (ABI User Bulletin 2). For graphical representation, data were normalized to the sample with the lowest relative Kr-h1 levels (which therefore received the value 1), and fold differences in relative expression levels are depicted as mean ± se. For the statistical analyses we used the ΔCt values, which are normally distributed. The sequences of the Kr-h1 and EF1a primers are in Additional file 4, Table S2. We used 6-15 biological replicates for each sample group; the sample size for each experiment is presented in the Results section.
The influence of queen presence on brain Kr-h1levels in workers
We carried out two experiments to test the influence of the queen on Kr-h1 levels in workers. In the first experiment, we collected 1-day-old callow workers from mixed colony backgrounds (this is done because a single colony does not produce a sufficient number of newly emerged bees in a single day), paint-marked them on their thoraces, and assigned them randomly to one of the following social environments: 1) a queenright colony, or 2) orphan groups of three workers in wooden cages (10 × 11 × 5 cm) with a Plexiglas top (3 workers/cage). We placed the colonies and small groups in an environmental chamber (28 ± 1°C) and provisioned them with commercial sugar syrup (Polyam, Yad Mordechai, Israel) and ground pollen. Maintaining workers in small groups in cages mimics the social environment of a queenless colony but allows a higher proportion of workers to acquire the top (α) dominance rank [24, 26, 34, 57]. Using multiple small queenless groups therefore results in the production of a higher proportion of uninhibited bees, because dominant workers typically inhibit reproductive physiology and behavior in subordinate groupmates [25, 26, 38, 57]. Workers from the queenless groups and from the queenright colonies were collected after 4 days into liquid nitrogen. Previous studies indicate that at four days of age there are significant physiological and behavioral differences between queenless and queenright workers [23–26, 34, 58, 59]. Three trials were completed, for a total of 43 queenright and 33 queenless individuals. Brain Kr-h1 levels were quantified at NCSU using qRT-PCR, as described above. Data from individual trials were analyzed using a two-tailed t-test; data from all three trials were combined and analyzed in a mixed model ANOVA (proc mixed in SAS 9.2, SAS Institute Inc, Cary, NC) with treatment and trial as variables.
In the second experiment we compared workers that were reared in queenless and queenright colonies. We obtained 4 colonies, each with about 20 workers and a similar amount of developing brood. After one day of acclimation to the lab, we removed the queen from two randomly selected colonies, and within two hours introduced 12 callow bees (from a mixed origin pool of bees that emerged during the previous 24 hours) into each colony. In order to keep colony size and density intact, we removed a similar number of workers from each host colony . The bees were collected and analyzed as described above. Brain Kr-h1 levels were quantified at HUJI using qRT-PCR. For statistical analysis we used a one-way ANOVA with colony as a variable (SPSS 15.0).
The influence of task on brain Kr-h1levels
We placed incipient bumble bee colonies in an environmental chamber (28 ± 1°C). During the first one to three weeks the colonies were fed ad libitum with commercial syrup and fresh pollen (collected by honey bees). The nest box was connected to the outside by a clear plastic tube (~1 m length, 2 cm diameter) and food provisioning was gradually stopped. We performed three repetitions, each with a different colony (termed A, B, and C). We connected the colonies when they contained ~50 workers (Colony A, 18 days from the emergence of the first worker), ~70 workers (Colony B, 19 days after the emergence of the first worker), or 20 workers (Colony C, about two weeks after the emergence of the first worker). We performed detailed (1-2 hrs during the morning, and 1-2 hrs during the evening, at a similar time of day) daily observations, starting three to seven days after connecting the colony to the outside. We marked each bee with a number tag and observed the colonies for at least three consecutive days before collection. Foragers were defined as bees observed returning to the hive with conspicuous pollen loads on their hind legs (returning from a foraging trip) every day during the observation period while doing no, or very little, brood care; nurses were bees observed caring for the brood, and doing no, or very little, foraging during this period (for more details, see ). We collected samples for RNA analysis (nurses N = 7, 25, 19; forager N = 15, 14, and 10 from colonies A, B, and C, respectively) on the last observation day (approximately 32, 33, and 26 days after the emergence of the first worker for colonies A, B, and C, respectively). During this time the colonies were self supporting. Colonies were monitored for the remainder of their life cycle, and the presence of open egg cells was used to estimate the initiation of the "competition phase" (which is characterized by competition over reproduction between the queen and workers; [38, 61]). Based on this, Colony B was likely in the competition phase at the point of collection. Data were analyzed using two-tailed t-tests, and two-way ANOVA for data pooled from the three trials. In the first repetition (Colony A), we collected the samples in Israel and shipped them on dry ice to NCSU for gene expression analysis. In the second and third repetitions (Colonies B and C) we measured the RNA levels in Israel. We measured ovarian status and body size only for bees from Colony A and C.
The influence of dominance rank on brain Kr-h1levels in groups of queenless workers
One-day old workers from mixed colony backgrounds were paint-marked and placed in groups of three in small wooden cages in a constantly dark environmental chamber (28 ± 1°C, ~50%RH). Bees in each group were of similar body size and age. For each queenless group, at least two sets of observations were carried out (20 min each): the first on day three, and the second on day seven, just before collecting the bees into liquid nitrogen. Dominance index was calculated following the method described in . Briefly, for each encounter, we recorded which bee advanced and which retreated. The dominance index was defined as: 1 - retreats/total encounters. In addition we recorded all agonistic behaviors including threatening behavior and overt aggression [24, 62]. We classified the bees according to their dominance rank; the most dominant bee was dubbed "α", the median "β", and the lowest in the rank "γ". Brain RNA levels were analyzed only for groups in which the degree of aggressiveness matched the calculated dominance rank (the α bee is the most agonistically active) for a total of 12 groups (out of 13 groups tested). Brain Kr-h1 levels were quantified in Israel using qRT-PCR, as described above. Data were analyzed using a one-way ANOVA with dominance rank as the variable, and with a Chi-square goodness of fit test with the expected probability for dominant individual to have the highest brain Kr-h1 levels = 0.33.
The influence of juvenile hormone on Kr-h1levels in queenright workers
One-day-old workers from a mixed colony background were paint-marked and assigned randomly to one of the following treatments (N = 15-17 bees/treatment): 1) JH-treatment, we dissolved 70 μg JH-III (Sigma, cat J2000, purity ≥ 65%, lot# = 087K26321; St. Louis, MO) in 5 μl DMF (J.T Backer cat: 7032 - 1L lot: 0509710007 Dimethylformamide) and applied the solution to the dorsal part of the thorax following the method of . We used JHIII because it is the only JH analogue found in B. terrestris [23, 24]. 2) DMF treatment, same as in (1), but we treated the bees with 5 μl DMF; 3) untreated control, we handled the bees as in the two other groups, but did not treat them with any solution. Bees from all three treatments were introduced into a young queenright colony (containing a viable queen and before the competition phase); all the introduced workers were accepted to the host colonies with no evidence for aggression. After 3 days we collected eight to ten bees/treatment into liquid nitrogen for subsequent RNA analysis. At 7 days of age, we collected five to eight additional bees per group for the analysis of ovarian state and body size. We used a mixed model ANOVA with treatment as the variable for the analyses of gene expression data and body size, and the Kruskal Wallis nonparametric test for the analysis of ovarian state (all proc mixed in SPSS 15.0).
Alignments of Kr-h1orthologs
Sequences of 20 KR-H1 insect orthologs were obtained (Additional file 1,2 Fig. S1, GenBank accession numbers for all orthologs can be found below). For the Drosophila species, the isoforms orthologous to Drosophila melanogaster KR-H1β were used. The complete protein sequences were aligned using ClustalW packaging in BioEdit 7.0, using the full multiple alignment feature (Additional file 1,2 Fig. S1). Alignment parameters were set to BLOSUM protein weight matrix with a gap opening penalty set to 10.0 and gap extension penalty set at 0.05. Sequences were not aligned by eye given the high degree of divergence outside of conserved regions.
The relatively conserved zinc finger region, which spans 277 amino acids sites and is indicated by the red bar in Additional file 1,2 Fig. S1, was used for the phylogenetic analysis. The maximum likelihood tree was constructed using phyML 3.0, under a fixed model of amino acid substitutions, allowing for substitution rate variation among site with a gamma distribution [65–67]. The tree was drawn in MEGA4 . Acyrthosiphon pisum (Homoptera) was used as the outgroup to the holometabolous insects based on well-established species phylogenetic data [36, 37, 69–71]. Branch support values are indicated at each node are from an analysis of 1000 bootstrap replicates. The WAG model of amino acid evolution was used . The final tree was uploaded into treeBASE (Accession number SN4664).
Accession numbers are as follows: pea aphid, Acyrthosiphon pisum (XM001946159); Aedes aegypti mosquito (EAT46451); Anopheles gambiae mosquito (EAA13888); honey bee, Apis melllifera (AAR08420); Bombus terrestris (GQ903677); Culex quinquefasciatus mosquito (EDS38735); Drosophila ananassae (EDV31532); Drosophila erecta (EDV59037); Drosophila grimshawi (EDW02871); Drosophila melanogaster (CAA06543); Drosophila mojavensis (EDW11744); Drosophila persimilis (EDW28206); Drosophila pseudoobscura (EAL34248); Drosophila sechellia (EDW54474); Drosophila simulans (EDX03916); Drosophila virilis (EDW63622); Drosophila willistoni (EDW76311); Drosophila yakuba (EDW87936); Nasonia vitripennis wasp (XP001600294); and the red flour beetle, Tribolium castaneum (AB360764).
The authors would like to thank Noa Kahana and Michal Merling for assistance with the bees. We thank members of the Grozinger laboratory for helpful discussions and critical reading of the manuscript. This work was supported by an NSF CAREER grant (0746338) and by funding from North Carolina State University to CMG, grants from the US-Israel Binational Science Foundation (BSF, #2007465), and Israel Science Foundation (ISF, #452/07) to GB, and a "Hoffman Leadership and Responsibility" fellowship to HS.
- Robinson GE, Grozinger CM, Whitfield CW: Sociogenomics: social life in molecular terms. Nat Rev Genet. 2005, 6 (4): 257-270. 10.1038/nrg1575.View ArticlePubMedGoogle Scholar
- Wilson EO: The Insect Societies. 1971, Cambridge, MA: The Belkap Press of Harvard University PressGoogle Scholar
- Michener CD: The Social Behavior of Bees. 1974, Cambridge: Harvard University PressGoogle Scholar
- Gadagkar R: The evolution of communication and the communication of evolution: The case of the honey bee queen pheromone. Orientation and Communication in Arthropods. Edited by: Lehrer M. 1997, Basel/Switzerland: Birkhauser Verlag, 375-395.View ArticleGoogle Scholar
- Keller L, Nonacs P: The role of queen pheromones in social insects: queen control or queen signal?. Anim Beh. 1993, 45: 787-794. 10.1006/anbe.1993.1092.View ArticleGoogle Scholar
- Robinson GE, Fernald RD, Clayton DF: Genes and social behavior. Science. 2008, 322 (5903): 896-900. 10.1126/science.1159277.PubMed CentralView ArticlePubMedGoogle Scholar
- Kravitz EA: Serotonin and aggression: insights gained from a lobster model system and speculations on the role of amine neurons in a complex behavior. J Comp Physiol [A]. 2000, 186 (3): 221-238. 10.1007/s003590050423.View ArticleGoogle Scholar
- Edwards DH, Spitzer N: 6. Social dominance and serotonin receptor genes in crayfish. Curr Top Dev Biol. 2006, 74: 177-199. 10.1016/S0070-2153(06)74006-6.View ArticlePubMedGoogle Scholar
- Trainor BC, Hofmann HA: Somatostatin and somatostatin receptor gene expression in dominant and subordinate males of an African cichlid fish. Behav Brain Res. 2007, 179 (2): 314-320. 10.1016/j.bbr.2007.02.014.PubMed CentralView ArticlePubMedGoogle Scholar
- White SA, Nguyen T, Fernald RD: Social regulation of gonadotropin-releasing hormone. J Exp Biol. 2002, 205 (Pt 17): 2567-2581.PubMedGoogle Scholar
- Gotzek D, Ross KG: Genetic regulation of colony social organization in fire ants: an integrative overview. Q Rev Biol. 2007, 82 (3): 201-226. 10.1086/519965.View ArticlePubMedGoogle Scholar
- Le Conte Y, Hefetz A: Primer pheromones in social hymenoptera. Annu Rev Entomol. 2008, 53: 523-542. 10.1146/annurev.ento.52.110405.091434.View ArticlePubMedGoogle Scholar
- Pankiw T, Huang Z, Winston ML, Robinson GE: Queen mandibular gland pheromone influences worker honey bee (Apis mellifera L.) foraging ontogeny and juvenile hormone titers. J Insect Physiol. 1998, 44 (7-8): 685-692. 10.1016/S0022-1910(98)00040-7.View ArticlePubMedGoogle Scholar
- Alaux C, Jaisson P, Hefetz A: Queen influence on worker reproduction in bumblebees (Bombus terrestris) colonies. Insect Sociaux. 2004, 51: 287-293. 10.1007/s00040-004-0741-5.View ArticleGoogle Scholar
- Lopez-Vaamonde C, Brown RM, Lucas ER, Pereboom JJM, Jordan WC, Bourke AFG: Effect of the queen on worker reproduction and new queen production in the bumble bee Bombus terrestris. 2007, 38: 171-180.Google Scholar
- Alaux C, Jaisson P, Hefetz A: Regulation of worker reproduction in bumblebees (Bombus terrestris): workers eavesdrop on a queen signal. Behav Ecol Sociobiol. 2006, 60: 439-446. 10.1007/s00265-006-0184-2.View ArticleGoogle Scholar
- Bloch G, Hefetz A: Reevaluation of the role of mandibular glands in regulation of reproduction in bumblebee colonies. J Chem Ecol. 1999, 25 (4): 881-896. 10.1023/A:1020805103379.View ArticleGoogle Scholar
- Sramkova A, Schulz C, Twele R, Francke W, Ayasse M: Fertility signals in the bumblebee Bombus terrestris (Hymenoptera: Apidae). Naturwissenschaften. 2008, 95 (6): 515-522. 10.1007/s00114-008-0353-4.View ArticlePubMedGoogle Scholar
- Bloch G, Wheeler DE, Robinson GE: Endocrine influences on the organization of insect societies. Hormones, Brain and Behavior. Non-mammalian hormone-behavior systems. Edited by: Pfaff D. 2002, II: 195-235.View ArticleGoogle Scholar
- Kaatz HH, Hildebrandt H, Engels W: Primer effect of queen pheromone on juvenile hormone biosynthesis in adult worker honey bees. J Comp Physiol [B]. 1992, 588-592. 162
- Huang Z-Y, Robinson GE, Brost DW: Physiological correlates of division of labor among similarly aged honey bees. J Comp Physiol [A]. 1994, 174: 731-739.View ArticleGoogle Scholar
- Sullivan JP, Fahrbach SE, Robinson GE: Juvenile hormone paces behavioral development in the adult worker honey bee. Horm Behav. 2000, 37 (1): 1-14. 10.1006/hbeh.1999.1552.View ArticlePubMedGoogle Scholar
- Bloch G, Borst DW, Huang Z, Robinson GE, Cnaani J, Hefetz A: Juvenile hormone titers, juvenile hormone biosynthesis, ovarian development and social environment in Bombus terrestris. J Insect Physiol. 2000, 46 (1): 47-57. 10.1016/S0022-1910(99)00101-8.View ArticlePubMedGoogle Scholar
- Bloch G, Borst DW, Huang Z-Y, Robinson GE, Hefetz A: Effects of social conditions on juvenile hormone-mediated reproductive development in Bombus terrestris workers. Physiological Entomology. 1996, 21: 257-267. 10.1111/j.1365-3032.1996.tb00863.x.View ArticleGoogle Scholar
- Roseler PF: Juvenile hormone control of oogenesis in bumblebee workers, Bombus terrestris. J Insect Physiol. 1977, 23: 985-992. 10.1016/0022-1910(77)90126-3.View ArticleGoogle Scholar
- Van Doorn A: Factors influencing dominance behaviour in queenless bumblebee workers (Bombus terrestris). Physiological Entomology. 1989, 14 (2): 211-221. 10.1111/j.1365-3032.1989.tb00954.x.View ArticleGoogle Scholar
- Cameron SA, Robinson GE: Juvenile hormone does not affect division of labor in Bumble bee colonies (Hymenoptera: Apidae). Ann Entomol Soc Am. 1990, 83: 626-631.View ArticleGoogle Scholar
- Van Doorn A: Investigations into the regulation of dominance behavior and the division of labor in bumblebee colonies (Bombus terrestris). Netherland Journal of Zoology. 1987, 37 (3-4): 255-276.Google Scholar
- Bloch G, Shpigler H, Wheeler DE, Robinson GE: Endocrine influences on the organization of insect societies. Hormones, Brain and Behavior. Edited by: Pfaff DW, Arnold AP, Etgen AM, Fahrbach SE, Rubin RT. 2009, San Diego: Academic Press, 2: 1027-1068. full_text. 2View ArticleGoogle Scholar
- Grozinger CM, Fischer P, Hampton JE: Uncoupling primer and releaser responses to pheromone in honey bees. Naturwissenschaften. 2007, 94 (5): 375-379. 10.1007/s00114-006-0197-8.View ArticlePubMedGoogle Scholar
- Grozinger CM, Robinson GE: Endocrine modulation of a pheromone-responsive gene in the honey bee brain. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2007, 193 (4): 461-470. 10.1007/s00359-006-0202-x.View ArticlePubMedGoogle Scholar
- Grozinger CM, Sharabash NM, Whitfield CW, Robinson GE: Pheromone-mediated gene expression in the honey bee brain. Proc Natl Acad Sci USA. 2003, 100 (Suppl 2): 14519-14525. 10.1073/pnas.2335884100.PubMed CentralView ArticlePubMedGoogle Scholar
- Wagner GP, Lynch VJ: The gene regulatory logic of transcription factor evolution. Trends in Ecology and Evolution. 2008, 23 (7): 377-385. 10.1016/j.tree.2008.03.006.View ArticlePubMedGoogle Scholar
- Duchateau MJ, Velthuis HHW: Ovarian development and egg laying in workers of Bombus terrestris. Entomol Exp Appl. 1989, 51: 199-213. 10.1007/BF00347883.View ArticleGoogle Scholar
- Iuchi S: Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci. 2001, 58 (4): 625-635. 10.1007/PL00000885.View ArticlePubMedGoogle Scholar
- Savard J, Tautz D, Richards S, Weinstock GM, Gibbs RA, Werren JH, Tettelin H, Lercher MJ: Phylogenomic analysis reveals bees and wasps (Hymenoptera) at the base of the radiation of Holometabolous insects. Genome Res. 2006, 16: 1334-1338. 10.1101/gr.5204306.PubMed CentralView ArticlePubMedGoogle Scholar
- Wiegmann BM, Trautwein MD, Kim JW, Cassel BK, Bertone MA, Winterton SL, Yeates DK: Single-copy nuclear genes resolve the phylogeny of the holometabolous insects. BMC Biol. 2009, 7: 34-10.1186/1741-7007-7-34.PubMed CentralView ArticlePubMedGoogle Scholar
- Bloch G, Hefetz A: Regulation of reproduction by dominant workers in bumblebee colonies (Bombus terrestris) queenright colonies. Beh Ecol Sociobiol. 1999, 45: 125-135. 10.1007/s002650050546.View ArticleGoogle Scholar
- Cumber RA: The biology of humble-bees, with special reference to the production of the worker caste. Trans R Ent Soc Lond. 1949, 100: 1-45.View ArticleGoogle Scholar
- Grozinger CM, Fan Y, Hoover SE, Winston ML: Genome-wide analysis reveals differences in brain gene expression patterns associated with caste and reproductive status in honey bees (Apis mellifera). Mol Ecol. 2007, 16 (22): 4837-4848. 10.1111/j.1365-294X.2007.03545.x.View ArticlePubMedGoogle Scholar
- Kocher SD, Richard FJ, Tarpy DR, Grozinger CM: Genomic analysis of post-mating changes in the honey bee queen (Apis mellifera). BMC Genomics. 2008, 9: 232-10.1186/1471-2164-9-232.PubMed CentralView ArticlePubMedGoogle Scholar
- Fussnecker B, Grozinger C: Dissecting the role of Kr-h1 brain gene expression in foraging behavior in honey bees (Apis mellifera). Insect Mol Biol. 2008, 17: 515-522. 10.1111/j.1365-2583.2008.00819.x.View ArticlePubMedGoogle Scholar
- Fahrbach SE, Farris SM, Sullivan JP, Robinson GE: Limits on volume changes in the mushroom bodies of the honey bee brain. J Neurobiol. 2003, 57: 141-151. 10.1002/neu.10256.View ArticlePubMedGoogle Scholar
- Withers GS, Fahrbach SE, Robinson GE: Effects of experience and juvenile hormone on the organization of the mushroom bodies of honey bees. J Neurobiol. 1995, 26: 130-144. 10.1002/neu.480260111.View ArticlePubMedGoogle Scholar
- Shi L, Lin S, Grinberg Y, Beck Y, Grozinger CM, Robinson GE, Lee T: Roles of Drosophila Kruppel-homolog 1 in neuronal morphogenesis. Dev Neurobiol. 2007, 67: 1614-1626. 10.1002/dneu.20537.View ArticlePubMedGoogle Scholar
- Minakuchi C, Zhou X, Riddiford LM: Kruppel homolog 1 (Kr-h1) mediates juvenile hormone action during metamorphosis of Drosophila melanogaster. Mech Dev. 2008, 125: 91-105. 10.1016/j.mod.2007.10.002.PubMed CentralView ArticlePubMedGoogle Scholar
- Minakuchi C, Namiki T, Shinoda T: Kruppel homolog 1, an early juvenile hormone-response gene downstream of Methoprene-tolerant, mediates its anti-metamorphic action in the red flour beetle Tribolium castaneum. Dev Biol. 2008, 325: 341-350. 10.1016/j.ydbio.2008.10.016.View ArticlePubMedGoogle Scholar
- Parthasarathy R, Tan A, Palli SR: bHLH-PAS family transcription factor methoprene-tolerant plays a key role in JH action in preventing the premature development of adult structures during larval-pupal metamorphosis. Mech Dev. 2008, 125: 601-616. 10.1016/j.mod.2008.03.004.PubMed CentralView ArticlePubMedGoogle Scholar
- Kraut R, Menon K, Zinn K: A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. Curr Biol. 2001, 11 (6): 417-430. 10.1016/S0960-9822(01)00124-5.View ArticlePubMedGoogle Scholar
- Carroll SB, Grenier JK, Weatherbee SD: From DNA to Diversity: Molecular Genetics and the Evolution of Animal Design. 2005, Malden, MA: Blackwell PublishingGoogle Scholar
- Toth AL, Robinson GE: Evo-devo and the evolution of social behavior. Trends Genet. 2007, 23: 334-341. 10.1016/j.tig.2007.05.001.View ArticlePubMedGoogle Scholar
- Robinson GE, Vargo EL: Juvenile hormone in adult eusocial Hymenoptera: gonadotropin and behavioral pacemaker. Arch Insect Biochem Physiol. 1997, 35 (4): 559-583. 10.1002/(SICI)1520-6327(1997)35:4<559::AID-ARCH13>3.0.CO;2-9.View ArticlePubMedGoogle Scholar
- West-Eberhard MJ: Wasp societies as microcosms for the study of development and evolution. Natural history and evolution of paper wasps. Edited by: Turillazzi S, West-Eberhard MJ. 1996, Oxford: Oxford University Press, 290-317.Google Scholar
- Hartfelder K, Engels W: Social insect polymorphism: hormonal regulation of plasticity in development and reproduction in the honeybee. Curr Top Dev Biol. 1998, 40: 45-77. 10.1016/S0070-2153(08)60364-6.View ArticlePubMedGoogle Scholar
- Yerushalmi S, Bodenhaimer S, Bloch G: Developmentally determined attenuation in circadian rhythms links chronobiology to social organization in bees. J Exp Biol. 2006, 209: 1044-1051. 10.1242/jeb.02125.View ArticlePubMedGoogle Scholar
- Bloch G, Toma DP, Robinson GE: Behavioral rhythmicity, age, division of labor and period expression in the honey bee brain. J Biol Rhythms. 2001, 16: 444-456. 10.1177/074873001129002123.View ArticlePubMedGoogle Scholar
- Cnaani J, Wong A, Thomson JD: Effect of group size on ovarian development in bumblebee workers (Hymenoptera: apidae: Bombus). Entomologia Generalis. 2007, 29: 305-314.View ArticleGoogle Scholar
- Bloch G, Simon T, Robinson GE, Hefetz A: Brain biogenic amines and reproductive dominance in bumble bees (Bombus terrestris). J Comp Physiol [A]. 2000, 186: 261-268. 10.1007/s003590050426.View ArticleGoogle Scholar
- Geva S, Hartfelder K, Bloch G: Reproductive division of labor, dominance, and ecdysteroid levels in hemolymph and ovary of the bumble bee Bombus terrestris. J Insect Physiol. 2005, 51: 811-823. 10.1016/j.jinsphys.2005.03.009.View ArticlePubMedGoogle Scholar
- Bloch G: Regulation of queen-worker conflict in bumble-bee (Bombus terrestris) colonies. Proc R Soc Lond B Biol Sci. 1999, 266: 2465-2469. 10.1098/rspb.1999.0947.View ArticleGoogle Scholar
- Duchateau MJ, Velthuis HHW: Development and reproductive strategies in Bombus colonies. Behavior. 1988, 107: 186-196. 10.1163/156853988X00340.View ArticleGoogle Scholar
- Van Doorn A, Heringa J: The ontogeny of a dominance hierarchy in colonies of the bumblebee Bombus terrestris (Hymenoptera, Apidae). Insect Sociaux. 1986, 33: 3-25. 10.1007/BF02224031.View ArticleGoogle Scholar
- Barron AB, Maleszka J, Meer Vander RK, Robinson GE, Maleszka R: Comparing injection, feeding and topical application methods for treatment of honeybees with octopamine. J Insect Physiol. 2007, 53: 187-194. 10.1016/j.jinsphys.2006.11.009.View ArticlePubMedGoogle Scholar
- Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleo Acids Symp Ser. 1999, 41: 95-98.Google Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704. 10.1080/10635150390235520.View ArticlePubMedGoogle Scholar
- Guindon S, Delsuc F, Dufayard JF, Gascuel O: Estimating maximum likelihood phylogenies with PhyML. Methods Mol Biol. 2009, 537: 113-137. full_text.View ArticlePubMedGoogle Scholar
- Jones DT, Taylor WR, Thornton JM: The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci. 1992, 8: 275-282.PubMedGoogle Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.View ArticlePubMedGoogle Scholar
- Wheeler WC, Whiting M, Wheeler QD, Carpenter JM: The phylogeny of the extant hexapod orders. Cladistics. 2001, 17: 113-169. 10.1111/j.1096-0031.2001.tb00115.x.View ArticleGoogle Scholar
- Boudreaux HB: Arthropod phylogeny with special reference to insects. 1979, New York: WileyGoogle Scholar
- Hennig W: Insect Phylogeny. 1981, New York: WileyGoogle Scholar
- Whelan S, Goldman N: A general empirical model of protein evolution derived from multiple protein families using a maximum-likelihood approach. Mol Biol Evol. 2001, 18 (5): 691-699.View ArticlePubMedGoogle Scholar
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