The role of historical and contemporary processes on phylogeographic structure and genetic diversity in the Northern Cardinal, Cardinalis cardinalis
© Smith et al; licensee BioMed Central Ltd. 2011
Received: 15 January 2011
Accepted: 20 May 2011
Published: 20 May 2011
Earth history events such as climate change are believed to have played a major role in shaping patterns of genetic structure and diversity in species. However, there is a lag between the time of historical events and the collection of present-day samples that are used to infer contemporary population structure. During this lag phase contemporary processes such as dispersal or non-random mating can erase or reinforce population differences generated by historical events. In this study we evaluate the role of both historical and contemporary processes on the phylogeography of a widespread North American songbird, the Northern Cardinal, Cardinalis cardinalis.
Phylogenetic analysis revealed deep mtDNA structure with six lineages across the species' range. Ecological niche models supported the same geographic breaks revealed by the mtDNA. A paleoecological niche model for the Last Glacial Maximum indicated that cardinals underwent a dramatic range reduction in eastern North America, whereas their ranges were more stable in México. In eastern North America cardinals expanded out of glacial refugia, but we found no signature of decreased genetic diversity in areas colonized after the Last Glacial Maximum. Present-day demographic data suggested that population growth across the expansion cline is positively correlated with latitude. We propose that there was no loss of genetic diversity in areas colonized after the Last Glacial Maximum because recent high-levels of gene flow across the region have homogenized genetic diversity in eastern North America.
We show that both deep historical events as well as demographic processes that occurred following these events are critical in shaping genetic pattern and diversity in C. cardinalis. The general implication of our results is that patterns of genetic diversity are best understood when information on species history, ecology, and demography are considered simultaneously.
A multitude of studies of various North American taxa have shown that historical events, such as Pleistocene climatic changes coupled with topographic, hydrologic and ecological barriers, were instrumental in generating phylogeographic structure within species [1–4]. From the extensive body of phylogeographic work on North American species, two generalizations have emerged 1) taxa were isolated into independently evolving lineages (i.e. species, subspecies, phylogroups) during the Pleistocene  and 2) genetic diversity within species is highest in areas that remained stable (refugia) through glacial cycles . Ultimately, these hypothesized predictions seek to link the phylogeographic structure in species to historical events.
A recent and critical historical event was the Last Glacial Maximum, a time when ice sheets covered much of present-day temperate North America. Entire biotas were fragmented and displaced to more southern latitudes . After the glaciers receded certain species colonized these newly available areas by rapid long-distance dispersal, a phenomenon referred to as the leading edge or pioneer model . A major prediction of the leading edge model is that genetic diversity will be lower in the recently colonized areas. This idea is supported by the observation that many species have higher genetic diversity in European refugial areas . Additionally, genetic data indicate that historical population expansions occurred in many species, a finding consistent with species colonizing newly available areas with suitable habitat. A goal of phylogeography is to recover evidence of population expansions; however, the lag between earlier population expansions and the present represents a time when ongoing demographic processes can either erase earlier genetic signatures of population structure or expansions or prevent such signatures from developing at all.
Contemporary processes can affect the spatial distribution of genetic diversity in various ways. High levels of gene flow may homogenize haplotype diversity throughout a population that once showed genetic structure. Conversely, nonrandom mating with individuals in close geographic proximity can generate genetic structuring within a continuous population . Autecology also plays a critical role in how species maintain genetic connectivity across the landscape [9, 10]. Vagile species with high ecological thresholds will be able spread haplotypes across an area more readily than poor dispersers with narrow ecological requirements. Interpretations of genetic diversity patterns are further confounded by changes in gene flow due to oscillations in population densities and by shifts in the connectivity of habitats. These points highlight the complexity of population-level processes and emphasize that genetic patterns of diversity may become so tightly coupled to on-going processes that signatures of historical differences or similarities may be erased.
To examine the impact of historical and contemporary processes on genetic pattern and diversity, we investigated a broadly distributed North American songbird, the Northern Cardinal (Cardinalis cardinalis). Cardinals are distributed from southeastern Canada, throughout the eastern United States, and in the southwestern states south through the Mexican lowlands to northern Central America . Cardinalis cardinalis is currently divided into 18 subspecies representing four subspecies groups . The species inhabits multiple biomes which include deserts, dry and humid tropical forests, and deciduous temperate forests. In all regions it prefers similar habitats of shrubs, small trees, forest-edge, or secondary growth .
In this study we use mitochondrial DNA (mtDNA) sequences, ecological niche models, and demographic data to test the impacts of historical and contemporary processes on pattern of present-day genetic differentiation and diversity in C. cardinalis. First, we evaluated whether historical events had generated deep phylogeographic structure or monophyletic groups in mtDNA across the range of cardinals. Second, we tested for the signature of historical demographic expansions that should have been generated in populations that expanded following glacial retreat. Third, we used present-day and paleoecological niche models to explore if clades had geographically stable ranges since the Last Glacial Maximum. Finally, we evaluate if genetic diversity patterns can be explained by contemporary demographic processes.
Genetic Diversity and Pattern
Clade genetic and range characteristics
Range Size (sq km)
AMOVA summary for the cardinalis and igneus clades
Source of Variation
Variance of Components
% of Variation
Within clade genetic diversity
# of Ind
# of Hap
Baja California Sur
Hypothesis testing of historical demography for Northern Cardinal clades using different demographic models
Marginal Likelihood (S.E.)
log Bayes Factors
-2187.800 (+/- 0.336)
-2170.486 (+/- 0.354)
-2155.803 (+/- 0.411)
-1585.983 (+/- 0.316)
-1580.354 (+/- 0.350)
-1565.140 (+/- 0.364)
-1513.415 (+/- 0.311)
-1512.251 (+/- 0.254)
-1503.067 (+/- 0.303)
-1380.049 (+/- 0.174)
-1380.054 (+/- 0.166)
-1379.877 (+/- 0.170)
Ecological Niche Models
The paleoecological niche model showed a pronounced reduction in the current range of the cardinalis clade in the eastern half of the U. S. A., which suggested that our samples in Louisiana, the Southeast, eastern México and possibly Texas are from areas that were a putative refugium (Figure 4D) during the Last Glacial Maximum. The Last Glacial Maximum model also indicated that there were suitable conditions for the cardinalis clade across the Chihuahuan Desert into the Sonoran Desert and east of the Sierra Madre Oriental (Figure 4D). There appears to have been a reduction in suitable climatic conditions for cardinals in México, such as on Baja California and the Mexican Pacific coast, but there was not the extreme range reduction seen in the cardinals of eastern North America.
Spatial and Temporal Correlations
Historical Signal - Phylogeographic Structure
Recent historical events, particularly Pleistocene glacial-interglacial cycles, have had a critical impact on generating phylogeographic structure in species . Typically, phylogeographic studies have examined how species responded to these events within a single biome or biogeographic region [16–18]. Here, we report how a species distributed across multiple biomes and biogeographic regions of North America responded to the climatic oscillations of the Pleistocene. In the Northern Cardinal, we found six lineages that have been likely differentiating since the Pliocene. Each of the four continental clades is found in a well-established biogeographic area: the Mexican Pacific coast, the Sonoran and Peninsular deserts, eastern North America and the Yucatán Peninsula. Additionally, two insular lineages on Cozumel and the Tres Marías Islands were monophyletic.
Major geological and climatic barriers appear to define the ranges of the mainland clades. The distribution of carneus is constrained by the Sierra Madre del Sur to the east and habitat turnover to the north and south. Igenus shares a range with many co-distributed desert species that border the Sierra Madre Occidental to the east and extend to the northern and southern limits of the Sonoran Desert. This clade does not exhibit the deep genetic structure between the Sonoran and Peninsular desert populations found in other co-distributed species . However, low-levels of genetic structure were detected in igneus, which is likely due to recent differentiation of the putative subspecies found in the Sonoran Desert, Baja California Peninsula, and Tiburón Island . Present-day gene flow among cardinals in these areas is likely limited by their geographic isolation or by ecological constraints , but gene exchange among these groups could have been more regular during the Last Glacial Maximum when sea levels were lower  and populations were in closer proximity.
Igneus is replaced in the Chihuahuan Desert by cardinalis and haplotypes of both clades are found east and west of the Chihuahuan-Sonoran transition zone. This region has been identified as an area where many species exhibit genetic breaks and phylogroups often come into secondary contact . Although our sampling was limited in the transition zone, we found no evidence of geographic overlap in these haplotypes and our genetic data and ecological niche model support an earlier conclusion that these forms do not hybridize . The present-day ecological niche model found the geographic area between these clades unsuitable for either group, but the paleoecological niche model indicated a more continuous distribution across this gap during the Last Glacial Maximum.
The evolution of the cardinalis clade in eastern North America has been a history of population connectivity. Remarkably, cardinalis, with a range of over 3,000,000 km2, has no appreciable geographic structure in mtDNA, an unexpected pattern for a widely distributed clade that has several described subspecies. Comparative phylogeographic work on other widely distributed avian species has shown a similar pattern of unstructured mtDNA in eastern North American birds . Alternatively, a diverse array of taxa including birds distributed in the southeast U.S.A. exhibit distinct phylogeographic structure that was shaped by a diverse array of historical factors .
The range limits of cardinalis and coccineus in eastern México are not well understood, but the mtDNA break coincides with the biogeographic barrier separating the Yucatán Peninsula biotic province from surrounding regions . Morphological work has identified an area of turnover where cardinalis grades into coccineus in Tamaulipas . Based on genetic data presented, however, specimens from Tamaulipas and areas farther south in Querétaro and Veracruz, México all possessed cardinalis haplotypes. Much of the Gulf Coast along México is unsuitable for cardinals because it has been converted to agriculture or encompasses low-lying areas that periodically flood. The ecological niche model also showed that habitat along the Gulf Coast to be sub-optimal. Additionally, coccineus appears to be a recently diverged lineage and is paraphyletic with respect to the monophyletic saturatus, a pattern consistent with incomplete lineage sorting between young groups .
The two island lineages, mariae and saturatus, are currently classified as subspecies in the igneus and coccineus groups, respectively, and both are monophyletic, suggesting that they are independently evolving lineages. Despite Cozumel being only 20 kms from the mainland and remerging 120,000 years ago , its biota has become distinct, with eight endemic bird and mammal species. Mariae occurs on the Tres Marías Islands, a small island chain 100 km off the Pacific coast of México that was likely submerged in the Late Pleistocene  and has several endemic subspecies . Molecular dating indicated that the mean mtDNA estimates of these island lineages are "older" than the islands. The divergence of the Tres Marías Islands lineage from the mainland was not congruent with the island's geology; the 95% HPD (0.31-2.86 million years ago) covers a time period before the island was presumably under water. But, the low end of 95% HPD for the divergence of the Cozumel Island (0.07-1.06 million years ago) is consistent with geology. The incongruence between the ND2 gene tree and geology may be due to an elevated island substitution rate , an undetected time-dependent substitution rate , or coalescent variance . Understanding the age of theses island lineages will require more thorough molecular dating using multilocus data.
Mitochondrial DNA as a Phylogeographic Marker
Mitochondrial DNA is a powerful marker for identifying independently evolving populations within species because of its fast mutation rate and maternal inheritance . However, incomplete lineage sorting , rate variation , and selection  can limit the utility of mtDNA as an evolutionary marker. Overall, the phylogeographic structure presented in this study is largely congruent with the morphological groups previously described for the species. These mtDNA lineages reflect a long history of isolation and independent evolutionary trajectories that merit further evaluation using multilocus coalescent methods .
Genetic diversity estimated from mtDNA is impacted by a number of factors, such as population size, age, genetic drift and mutation rate . By using range size and genetic distances as proxies for population size and time of isolation, we were able to show that nucleotide diversity in C. cardinalis was more strongly coupled to range size than genetic distance. This relationship between range size and genetic diversity was expected because larger ranges have the potential for larger populations and thus are able to accrue higher levels of genetic diversity . But, this finding is important because it suggests that mtDNA genetic diversity is at least partially coupled with cardinal population size, and indicates that the estimates provided here have not been strongly affected by selection and linkage to the W sex chromosome  or genetic draft .
Post-glacial Expansion: Historical to Contemporary Demography
The leading edge model of post-glacial expansion predicts there will be lower genetic diversity in recently colonized regions . Despite strong evidence for this model in some taxa and some geographic areas, there has not been compelling evidence for this pattern in North American birds [, , ; but see ]. These results are often attributed to other factors such as mixing of separate refugial populations, un-sampled populations, or rapid expansions. Both our genetic data and our ecological niche modeling suggest a population expansion for the cardinalis.
Given that the cardinalis clade likely expanded out of a glacial refugium, it is surprising that we found nucleotide diversity, haplotype diversity and the frequency of private haplotypes to be uniform across cardinalis sampling localities. Intuitively, homogeneous genetic diversity would seem attributable to birds being more vagile than other vertebrates, but this is unlikely the case here. Many birds are constrained by the same ecological barriers as non-volant organisms . This ecological limitation of dispersal is evident in igneus, which has also undergone a recorded northern range expansion. Our igneus samples from Arizona/New Mexico had lower haplotype and genetic diversity than samples from Sinaloa. A critical difference between cardinalis and igneus is the amount of habitat connectivity across their ranges. There are few major dispersal barriers for cardinals in eastern North America, allowing the species to freely disperse in any direction. On the other hand, in the areas of the arid Sonoran Desert where cardinals have recently colonized, they are more restricted to habitats with suitable vegetation such as riparian zones.
The high genetic diversity in recently established populations of the eastern cardinalis contradicts the leading edge model and begs explanation. One suggested alternative is the Phalanx model, which posits that large populations expanded slowly into newly available habitats with no loss of genetic diversity in newly colonized areas . Although the Phalanx predicts homogenous genetic diversity as seen in cardinalis, we see no reason for cardinals to have expanded their range slowly. Indeed, the rapid northward range expansion documented in the last 150 years suggests cardinals have high dispersal rates and can rapidly populate suitable habitat. Why may this be the case?
We suggest that cardinals in eastern North America were held at low numbers by a shortage of suitable habitat prior to the European settlement of North America. Throughout their range cardinals inhabit brushy open habitat, and prior to European settlement this ecotonal species lived in a sea of eastern hardwood forest where suitable habitat was patchy and ephemeral, created by water courses, tree falls, and fires. Throughout most of the evolutionary history of eastern cardinals, suitable habitat must have been ephemeral, leading to selection for high dispersal ability. Once European settlement converted most of Eastern North America into a vast ecotone of excellent cardinal habitat, their evolved propensity for high dispersal would homogenize genetic diversity across vast areas.
The hypothesis that cardinals exhibit high dispersal abilities is borne out by an analysis of banding records for cardinals . Although conducted before root mean square dispersal was being estimated, Dow and Scott  found that 190 of 1523 recovered cardinals were found outside the 10-minute block of latitude and longitude where they were banded; among those that moved beyond their block, first years moved an average distance of 60 km and adults moved an average of 130 km . These are large distances for a resident bird , and comparing them with results for North American blackbirds analyzed in modern ways  suggests that root mean square dispersal for Northern Cardinals is very high, possibly exceeding 100 km. Dispersal distances of this magnitude would be sufficient to homogenize genetic variation in a short period of time.
What these data suggest is that during the lag between historical expansion and the present-day, contemporary demography has played a role in shaping the observed genetic diversity pattern. Population densities are lowest at higher latitudes and population growth increases with latitude. We suggest that this population growth is not just from offspring staying in one area and increasing the density of individuals. Instead, populations are also growing as new individuals with different haplotypes are moving into new areas. Given that genetic diversity is uniform even in the youngest cardinal populations, at the highest latitudes, the lag between expansion and genetic homogenization may be on the order of decades instead of hundreds or thousands of years. As new areas became inhabitable, dispersal would likely not have been just in a northern and linear fashion, but east and west. If cardinals expanded out of southern glacial refuges rapidly and in large numbers, the signature of decreased genetic diversity may have been rapidly erased or alternatively, potentially never existed at all.
In this study we used a widely distributed songbird, the Northern Cardinal, Cardinalis cardinalis, as a model to test historical predictions for how species evolved in response to Pleistocene glacial-interglacial cycles. We found evidence in the mitochondrial DNA and from our modeled distribution of the species that there is deep phylogeographic structure in C. cardinalis, which is consistent with fragmentation caused by historical climate change. This structure likely began developing well before the Late Pleistocene and is supported by the results of both contemporary and paleoecological niche models. A modeled paleodistribution along with historical demographic hypothesis tests indicated that cardinals expanded out of refugia in eastern North America since the Last Glacial Maximum. However, there is no signature of decreased genetic diversity in areas colonized after the expansion. We suggest on-going gene flow across eastern North America has likely homogenized genetic diversity across the region. These results demonstrate that both Earth history events and cotemporary processes are important in determining the geography of genetic diversity observed within species.
We obtained samples from 163 individuals of Cardinalis cardinalis that includes representatives from 14 of the 18 recognized subspecies and the four subspecies groups: carneus, igneus, coccineus, and cardinalis (Additional file 1). We included six closely related species  in our dataset; C. sinuatus, C. phoeniceus, Rhodothraupis celaeno, Periporphyrus eyrthromelas, Caryothraustes poliogaster, and C. canadensis) and the more distantly related Molothrus ater as the outgroup. We extracted total genomic DNA from tissues and toe-pads from voucher specimens using the DNeasy tissue extraction kit (Qiagen, Valencia, CA). We amplified the mitochondrial gene NADH dehydrogenase subunit II (ND2) via polymerase chain reaction (PCR) using the primers L5215  and HTrpc (STRI). We used 12.5 μl reactions using the following protocol: denaturation at 94°C for 10 min, 40 cycles of 94°C for 30 s, 54°C for 45 s, and 72°C for 2 min, followed by a final 10 min elongation at 72°C. PCR products were sent to High-Throughput Genomics Unit (University of Washington, Seattle) for all subsequent sequencing steps. PCR products were purified using ExoSAP-IT (USB Corporation, Cambridge, MA), run through cycle-sequencing reactions and final products were sequenced using BigDye (Applied Biosystems, Foster City, CA) on a high-throughput capillary sequencer. We aligned chromatograms of the forward and reverse strands in Sequencher 4.9 (GeneCodes Corporation, Ann Arbor, MI) and sequences were translated into amino acids to check for premature stop codons.
We used several approaches to get an understanding of the distribution of genetic diversity within C. cardinalis. Aligned sequences were run through the program Mr. Modeltest v. 2.3  to estimate an appropriate model of sequence evolution. We determined the sequence evolution model for the complete ND2 gene as well as data partitioned by codon position (1st and 2nd codon positions and 3rd codon position). The best fit sequence model was determined by use of the Akiake Information Criterion (AIC). We constructed a gene tree using the program, MrBayes v. 3.1.2 . We constructed gene trees with the non-partitioned data and partitioned by codon positions. The branch length prior was set to unconstrained with an exponential distribution with the parameter set to 100.0 to avoid artificially long branches . All priors were unlinked for the partitioned data and we ran this analysis for seven million generations and sampled every 1000. We performed diagnostic tests to evaluate mixing and convergence of MCMC chains and the burn-in was determined from visual inspection of the likelihood plots in the program Tracer v 1.5 . To determine which approach was the best fit to the data, we used Bayes factors, computed from the harmonic mean of the non-partitioned and partitioned posterior likelihoods. We estimated divergence times using the program BEAST v. 1. 6. 1  and ran the analyses with a fixed substitution rate of 1.23 × 10-2 subs/site/lineage/million years, a rate estimated relative to the avian "2% rule"  used for the gene cytochrome-b [see ]. We unlinked ND2 codon positions, and used a Yule speciation tree prior and a relaxed uncorrelated molecular clock (lognormal distribution, mean 0.0123, SD = 0.45). The analysis was run for 50 million generations and the posterior output was examined in Tracer v 1.5  to assess mixing and convergence of MCMC chains.
Clades identified from our gene tree were used to estimate the genetic distance (internode to clade node distance) using an uncorrected p-distance for each clade in the program MEGA 4 . Two of the clades, igneus and cardinalis have relatively large ranges and are comprised of several subspecies; therefore we examined the amount of genetic structure within and among subpopulations for each of these clades by performing an AMOVA (Analysis of Molecular Variance) in the program Arlequin v. 3.11 . For the group designation for the AMOVA, we used U. S. states for cardinalis and for igneus we used the regions Baja California Sur, Tiburón Island, Arizona/New Mexico and Sinaloa. We calculated genetic diversity (the average number of nucleotide differences per site between two sequences), haplotype diversity , and the frequency of private haplotypes in the program DNAsp v. 5.10.01  for each clade and for subpopulations that had adequate sample sizes (n ≥ 8). Sample sizes among clades were skewed; therefore we estimated nucleotide diversity with all available samples in a clade and with equal samples (n = 6).
To perform hypothesis tests on cardinal historical demography, we re-estimated sequence models for each individual clade that had adequate sampling (cardinalis, coccineus, and igneus). We evaluated demographic history in the program BEAST v. 1. 5.4  by comparing the likelihood of three different models for the coalescent tree prior - constant population size, expansion growth, and Bayesian skyline. This approach allowed us to use Bayes factors to test whether a population expansion was more likely than a constant population size . Given that the approach used to estimate Bayes factors in Tracer has been shown to be yield unstable results [12–14], we additionally evaluated demographic history by examining Bayesian skyline plots, which shows a visual representation of population change over time. We used the same parameter settings (except we used a strict molecular clock for this analysis) and MCMC diagnostics as our BEAST divergence time analysis. Bayes factors were calculated by manually summing the tree likelihood and coalescent/skyline columns in the BEAST log file.
Ecological Niche Models
We downloaded locality records from two online databases, ORNIS (museum records only http://ornisnet.org) and observation records from the Avian Knowledge Network http://www.avianknowledge.net/content and plotted all records to get an estimate of the C. cardinalis distribution. To build an ecological niche model for C. cardinalis, we used only records from observations and specimens collected for this study. We assumed that these locality records are the most precise because they have been collected in the last ten years. The number of observation records for C. cardinalis was heavily skewed towards records in eastern North America where it is a common "backyard" bird. Therefore, we randomly sampled a subset of the records in order to have a more even sampling scheme. In total we compiled 664 records for C. cardinalis.
Beginning in the late 1800 s a well-documented northward range expansion has been recorded for C. cardinalis in the northern U. S. A. and the Colorado River Basin [11, 62]. We built additional ecological niche models that attempted to approximate the pre-expansion distribution by making an estimate of their pre-19th century range. The specific limits of the species' range prior to expansion are not precisely known. Therefore, based on distribution records , we used 40° N as a conservative and rough estimate of cardinal's pre-19th century range (post-glacial) in eastern North America and 33° N in the southwest U. S. A. All records above these latitudes were not included in the analysis in the post-glacial ecological niche models.
We used the Bioclimatic variables from the WorldClim dataset (v. 1.4) with a resolution of 2.5 min . Eight of the variables were correlated with other variables (R> 0.90); therefore, we used 11 of the 19 temperature and precipitation variables (BIO1, BIO2, BIO5, BIO6, BIO8, BIO9, BIO10, BIO12, BIO13, BIO15 and BIO18). We generated five replicate models for each separate clade identified from our phylogenetic analysis using the maximum entropy algorithm in MAXENT 3.3.3 . The model was then applied to the Model for Interdisciplinary Research on Climate (MIROC) layers to estimate suitable climatic conditions for C. cardinalis during the Last Glacial Maximum. We visualized models in the program ArcGIS 9.3 (ESRI Inc., Redlands, CA). Because the distribution of C. cardinalis is well known we used a digital range map  to set the logistic threshold values for the climatic suitability for each clade and applied these same threshold values to the paleoecological niche models. To calculate range size of each clade, we converted the model raster files into polygons in ArcGIS 9.3 and recorded polygon area in square kilometers.
To evaluate the impact of contemporary demography on genetic diversity, we used data from the North American Breeding Bird Survey, a long-term demographic study on breeding birds in the USA and Canada . Breeding Bird Survey data is based on mean counts of species along specified routes from these data density and population growth estimates are approximated. The datasets we used were population growth (% change over time) and population density estimates. We used population growth based on estimates of demographic trends of this species in a U. S. state over 40 years (1966-2007). For population densities, we directly extracted values from the Summer Distribution Map 1994-2003 using the average latitude from our genetic samples.
We thank R. Bryson Jr., J. DaCosta, G. Spellman, J. Batten, C. Tabone, UNLV Systematics Group, T. Monterrubio, J. Chaves, R. Canales, M. Gurrola, J. Rodríguez, J. Jaeger, and two anonymous reviewers. The following individuals and institutions provided samples for the study: Paul Sweet and George Barrowclough (American Museum of Natural History), Christopher Witt and Andrew Jones (Museum of Southwest Biology, University of New Mexico), Robert Zink (Bell Museum of Natural History), Sharon Birks (U of Washington, Burke Museum of Natural History), and Mark Robbins (University of Kansas). This work was partially funded by a UNLV International Studies Scholarship, a UNLV GPSA grant and an American Museum of Natural History Chapman Grant. This study was conducted in accordance with American and Mexican state and federal permits and in compliance with each government's animal research laws.
- Soltis DE, Morris AB, McLachlan JS, Manos PS, Soltis PS: Comparative phylogeography of unglaciated eastern North America. Mol Ecol. 2006, 15: 4261-4293. 10.1111/j.1365-294X.2006.03061.x.View ArticlePubMedGoogle Scholar
- Riddle BR, Hafner DJ, Alexander LF: Phylogeography and systematics of the Peromyscus eremicus species group and the historical biogeography of North American warm regional deserts. Mol Phyl Evol. 2000, 17: 145-160. 10.1006/mpev.2000.0841.View ArticleGoogle Scholar
- Zink RM, Kessen AE, Line TV, Blackwell-Rago RC: Comparative phylogeography of some aridland bird species. The Condor. 2001, 103: 1-10. 10.1650/0010-5422(2001)103[0001:CPOSAB]2.0.CO;2.View ArticleGoogle Scholar
- Swenson NG, Howard DJ: Clustering of contact zones, hybrid zones, and phylogeographic breaks in North America. Am Nat. 2005, 166: 581-91. 10.1086/491688.View ArticlePubMedGoogle Scholar
- Klicka J, Zink RM: The importance of recent ice ages in speciation: a failed paradigm. Science. 1997, 277: 1666-1669. 10.1126/science.277.5332.1666.View ArticleGoogle Scholar
- Hewitt G: The genetic legacy of the Quaternary ice ages. Nature. 2000, 405: 907-913. 10.1038/35016000.View ArticlePubMedGoogle Scholar
- Hewitt GM: Some genetic consequences of ice ages, and their role, in divergence and speciation. Biol J Linn Soc. 1996, 58: 247-276.View ArticleGoogle Scholar
- Slatkin M: Isolation by distance in equilibrium and non-equilibrium populations. Evolution. 1993, 47: 264-279. 10.2307/2410134.View ArticleGoogle Scholar
- Burridge CP, Craw D, Jack DC, King TM, Waters JM: Does fish ecology predict dispersal across a river drainage divide?. Evolution. 2008, 62: 1484-1499. 10.1111/j.1558-5646.2008.00377.x.View ArticlePubMedGoogle Scholar
- Burney CW, Brumfield RT: Ecology predicts levels of genetic differentiation in Neotropical birds. Am Nat. 2009, 174: 358-368. 10.1086/603613.View ArticlePubMedGoogle Scholar
- Halkin SL, Linville SU: Northern Cardinal (Cardinalis cardinalis) The Birds of North America No 44. Edited by: Poole A, Gill F. 1999, The Birds of North America Inc, PhiladelphiaGoogle Scholar
- Lartillot N, Philippe H: Computing Bayes factors using thermodynamic integration. Syst Biol. 2006, 55: 195-207. 10.1080/10635150500433722.View ArticlePubMedGoogle Scholar
- Wolpert RL, Schmidler SC: α-Stable limit laws for harmonic mean estimators of marginal likelihoods. 2010, Department of Statistical Science, Duke University, Durham, NC, 10-19.Google Scholar
- Xie W, Lewis PO, Fan Y, Kuo L, Chen MH: Improving marginal likelihood estimation for Bayesian phylogenetic model selection. Syst Biol. 2011, 60: 150-160. 10.1093/sysbio/syq085.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu L, Pearl D: Species trees from gene trees: reconstructing Bayesian posterior distributions of a species phylogeny using estimated gene tree distributions. Syst Biol. 2007, 56: 504-514. 10.1080/10635150701429982.View ArticlePubMedGoogle Scholar
- Houston D, Shiozawa DK, Riddle BR: Phylogenetic relationships of the western North American cyprinid genus Richardsonius, with an overview of phylogeographic structure. Mol Phyl Evol. 2010, 55: 259-273. 10.1016/j.ympev.2009.10.017.View ArticleGoogle Scholar
- Spellman GM, Klicka J: Testing hypotheses of Pleistocene population history using coalescent simulations: phylogeography of the pygmy nuthatch (Sitta pygmaea). Proc Roy Soc B Biol. 2006, 273: 3057-3063. 10.1098/rspb.2006.3682.View ArticleGoogle Scholar
- Arbeláez-Cortés E, Nyári ÁS, Navarro-Sigüenza AG: The differential effect of lowlands on the phylogeographic pattern of a Mesoamerican montane species (Lepidocolaptes affinis, Aves: Furnariidae). Mol Phyl Evol. 2010, 57: 658-668. 10.1016/j.ympev.2010.06.013.View ArticleGoogle Scholar
- Riddle BR, Hafner DJ, Alexander LF, Jaeger JR: Cryptic vicariance in the historical assembly of a Baja California peninsular desert biota. Proc Natl Acad Sci USA. 2000, 97: 14438-14443. 10.1073/pnas.250413397.View ArticlePubMedPubMed CentralGoogle Scholar
- Ridgway R: The birds of Middle and North America: a descriptive catalogue of the higher groups, genera, species, subspecies of birds known to occur in North America. U S Nat Mus Bull. 1901, 1: 50-Google Scholar
- Rojas-Soto OR, Westberg M, Navarro-Sigüenza AG, Zink RM: Genetic and ecological differentiation in the endemic avifauna of Tiburón Island. J Avian Biol. 2010, 41: 398-406. 10.1111/j.1600-048X.2010.04864.x.View ArticleGoogle Scholar
- Clark PU, Mix AC: Ice sheets and sea level of the Last Glacial Maximum. Quat Sci Rev. 2002, 21: 1-7. 10.1016/S0277-3791(01)00118-4.View ArticleGoogle Scholar
- Zink RM: Comparative phylogeography in North American birds. Evolution. 1996, 50: 308-317. 10.2307/2410802.View ArticleGoogle Scholar
- Vázquez-Domínguez E, Arita HT: The Yucatan peninsula: biogeographical history 65 million years in the making. Ecography. 2010, 33: 212-219.Google Scholar
- Parkes KC: The Northern Cardinals of the Caribbean slope of México, with the description of an additional subspecies from Yucatan. The era of Alan R Phillips: A festschrift. 1997, Compiler: Dickerman RW Horizon Communications, AlbuquegueGoogle Scholar
- Omland KE, Baker JM, Peters JL: Genetic signatures of intermediate divergence: population history of Old and New World Holarctic ravens (Corvus corax). Mol Ecol. 2006, 15: 795-808. 10.1111/j.1365-294X.2005.02827.x.View ArticlePubMedGoogle Scholar
- Spaw RH: Late Pleistocene carbonate bank deposition; Cozumel Island, Quintana Roo, Mexico. Gulf Coast Assoc Geol Soc Trans. 2006, 28: 601-619.Google Scholar
- McCloy C, Ingle JC, Barron JA: Neogene stratigraphy, foraminifera,diatoms, and depostional history of Maria Madre Island, Mexico: Evidence of Early Neogene marine conditions in the southern Gulf of California. Marine Micropaleontology. 1988, 13: 193-212. 10.1016/0377-8398(88)90003-5.View ArticleGoogle Scholar
- Stager KE: The avifauna of the Tres Marías Islands, México. The Auk. 1957, 74: 413-432.View ArticleGoogle Scholar
- Woolfit M, Bromham L: Population size and the rate of molecular evolution on islands. Proc Roy Soc Lond B. 2005, 272: 2277-2282. 10.1098/rspb.2005.3217.View ArticleGoogle Scholar
- Ho SYW, Phillips MJ, Cooper A, Drummond AJ: Time dependency of molecular rate estimates and systematic overestimation of recent divergence times. Mol Biol Evol. 2005, 22: 1561-1568. 10.1093/molbev/msi145.View ArticlePubMedGoogle Scholar
- Peterson GI, Masel J: Quantitative prediction of molecular clock and Ka/Ksat short timescales. Mol Biol Evol. 2009, 26: 2595-2603. 10.1093/molbev/msp175.View ArticlePubMedPubMed CentralGoogle Scholar
- Zink RM, Barrowclough GF: Mitochondrial DNA under siege in avian phylogeography. Mol Ecol. 2008, 17: 2107-2121. 10.1111/j.1365-294X.2008.03737.x.View ArticlePubMedGoogle Scholar
- Maddison WP: Gene trees in species trees. Syst Biol. 1997, 46: 523-536. 10.1093/sysbio/46.3.523.View ArticleGoogle Scholar
- Arbogast BS, Edwards SV, Wakeley J, Beerli P, Slowinski JB: Estimating divergence times from molecular data on phylogenetic and population genetic timescales. Annu Rev Ecol Syst. 2002, 33: 707-740. 10.1146/annurev.ecolsys.33.010802.150500.View ArticleGoogle Scholar
- Cheviron ZA, Brumfield RT: Migration-selection balance and local adaptation of mitochondrial haplotypes in Rufous-collared Sparrows (Zonotrichia capensis) along an elevational gradient. Evolution. 2009, 63: 1593-1605. 10.1111/j.1558-5646.2009.00644.x.View ArticlePubMedGoogle Scholar
- Leaché AD, Fujita MK: Bayesian species delimitation in west African forest geckos (Hemidactylus fasciatus). Proc Roy Soc B Biol. 2010, 277: 3071-3077. 10.1098/rspb.2010.0662.View ArticleGoogle Scholar
- Avise JC: Phylogeography: The History and Formation of Species. 2000, Harvard University Press, CambridgeGoogle Scholar
- Frankham R: Relationships of genetic variation to population size in wildlife. Cons Biol. 1996, 10: 1500-1508. 10.1046/j.1523-1739.1996.10061500.x.View ArticleGoogle Scholar
- Berlin S, Tomaras D, Charlesworth B: Low mitochondrial variability in birds may indicate Hill-Robertson effects on the W chromosome. Heredity. 2007, 99: 389-396. 10.1038/sj.hdy.6801014.View ArticlePubMedGoogle Scholar
- Bazin E, Glémin S, Galtier N: Population size does not influence mitochondrial genetic diversity in animals. Science. 2006, 312: 570-572. 10.1126/science.1122033.View ArticlePubMedGoogle Scholar
- Fry AJ, Zink RM: Geographic Analysis of Nucleotide Diversity and Song Sparrow Population History. Mol Ecol. 1998, 7: 1303-1313. 10.1046/j.1365-294x.1998.00462.x.View ArticlePubMedGoogle Scholar
- Milá B, Smith TB, Wayne RK: Speciation and rapid phenotypic differentiation in the yellow-rumped warbler (Dendroica coronata) complex. Mol Ecol. 2007, 16: 159-173.View ArticlePubMedGoogle Scholar
- Milá B, Girman DJ, Kimura M, Smith TB: Genetic evidence for the effect of a post-glacial population expansion on the phylogeography of a North American songbird. Proc Roy Soc B Biol. 2000, 267: 1033-1040. 10.1098/rspb.2000.1107.View ArticleGoogle Scholar
- Smith BT, Klicka J: The profound influence of the Late Pliocene Panamanian uplift on the exchange, diversification, and distribution of New World birds. Ecography. 2010, 33: 333-342.View ArticleGoogle Scholar
- Dow DD, Scott DM: Dispersal and range expansion by the cardinal: an analysis of banding records. Can J Zool. 1971, 49: 185-198. 10.1139/z71-028.View ArticlePubMedGoogle Scholar
- Paradis E, Baillie SR, Sutherland WJ, Gregory RD: Patterns of natal and breeding dispersal in birds. J Animal Ecol. 2002, 67: 518-536.View ArticleGoogle Scholar
- Moore WS, Dolbeer RA: The use of banding recovery data to estimate dispersal rates and gene flow in avian species: case studies in red-winged blackbird and common grackle. The Condor. 1989, 91: 242-253. 10.2307/1368301.View ArticleGoogle Scholar
- Klicka J, Burns K, Spellman GM: Defining a monophyletic Cardinalini: A molecular perspective. Mol Phyl Evol. 2007, 45: 1014-1032.View ArticleGoogle Scholar
- Hackett SJ: Molecular phylogenetics and biogeography of tanagers in the genus Ramphocelus (Aves). Mol Phyl Evol. 1996, 5: 368-382. 10.1006/mpev.1996.0032.View ArticleGoogle Scholar
- Nylander J: MrModeltest v 2.3. 2004, [http://www.abc.se/~nylander]Google Scholar
- Ronquist F, Huelsenbeck JP: MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinfor. 2003, 19: 1572-1574. 10.1093/bioinformatics/btg180.View ArticleGoogle Scholar
- Brown JM, Hedtke SM, Lemmon AR, Lemmon EM: When trees grow too long: investigating the causes of highly inaccurate Bayesian branch length estimates. Syst Biol. 2010, 59: 145-161. 10.1093/sysbio/syp081.View ArticlePubMedGoogle Scholar
- Rambaut A, Drummond AJ: Tracer v 1.5. 2010, [http://beast.bio.ed.ac.uk/]Google Scholar
- Drummond AJ, Rambaut A: BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol. 2007, 7: e214-10.1186/1471-2148-7-214.View ArticleGoogle Scholar
- Lovette IJ: Mitochondrial dating and mixed support for the "2% rule" in birds. Auk. 2004, 121: 1-6. 10.1642/0004-8038(2004)121[0001:MDAMSF]2.0.CO;2.Google Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.View ArticlePubMedGoogle Scholar
- Excoffier LGL, Schneider S: Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol Bioinfor Onl. 2005, 1: 47-50.Google Scholar
- Nei M: Molecular Evolutionary Genetics. 1987, Columbia Univ Press, New YorkGoogle Scholar
- Librado P, Rozas J: DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinfor. 2009, 25: 1451-1452. 10.1093/bioinformatics/btp187.View ArticleGoogle Scholar
- Suchard MA, Weiss RE, Sinsheimer JS: Bayesian selection of continuous-time Markov chain evolutionary models. Mol Biol Evol. 2001, 18: 1001-1013.View ArticlePubMedGoogle Scholar
- Monson G, Phillips AR: Annotated checklist of birds of Arizona. 1981, Univ of Arizona Press, Tucson, 2Google Scholar
- Hijmans RJ, Cameron SE, Parra JL, Jones PG, Jarvis A: Very high resolution interpolated climate surfaces for global land areas. Int J Clim. 2005, 25: 1965-1978. 10.1002/joc.1276.View ArticleGoogle Scholar
- Phillips SJ, Anderson RP, Schapire RE: Maximum entropy modeling of species geographic distributions. Ecol Mod. 2006, 190: 231-259. 10.1016/j.ecolmodel.2005.03.026.View ArticleGoogle Scholar
- Ridgely RS, Allnutt TF, Brooks T, McNicol DK, Mehlman DW, Young BE, Zook JR: Digital Distribution Maps of the Birds of the Western Hemisphere, version 3.0. NatureServe, Arlington. 2007Google Scholar
- Sauer JR, Hines JE, Fallon J: The North American Breeding Bird Survey, Results and Analysis 1966-2007. Version 5.15. USGS Patuxent Wildlife Research Center, Laurel, MD
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.