Expansion and diversification of the SET domain gene family following whole-genome duplications in Populus trichocarpa
© Lei et al; licensee BioMed Central Ltd. 2012
Received: 12 September 2011
Accepted: 12 April 2012
Published: 12 April 2012
Histone lysine methylation modifies chromatin structure and regulates eukaryotic gene transcription and a variety of developmental and physiological processes. SET domain proteins are lysine methyltransferases containing the evolutionarily-conserved SET domain, which is known to be the catalytic domain.
We identified 59 SET genes in the Populus genome. Phylogenetic analyses of 106 SET genes from Populus and Arabidopsis supported the clustering of SET genes into six distinct subfamilies and identified 19 duplicated gene pairs in Populus. The chromosome locations of these gene pairs and the distribution of synonymous substitution rates showed that the expansion of the SET gene family might be caused by large-scale duplications in Populus. Comparison of gene structures and domain architectures of each duplicate pair indicated that divergence took place at the 3'- and 5'-terminal transcribed regions and at the N- and C-termini of the predicted proteins, respectively. Expression profile analysis of Populus SET genes suggested that most Populus SET genes were expressed widely, many with the highest expression in young leaves. In particular, the expression profiles of 12 of the 19 duplicated gene pairs fell into two types of expression patterns.
The 19 duplicated SET genes could have originated from whole genome duplication events. The differences in SET gene structure, domain architecture, and expression profiles in various tissues of Populus suggest that members of the SET gene family have a variety of developmental and physiological functions. Our study provides clues about the evolution of epigenetic regulation of chromatin structure and gene expression.
Gene expression is regulated by many factors acting in concert with the status of the chromatin environment, particularly histone methylation . The SET-domain-containing protein family is a major player in histone methylation. These proteins are responsible for the methylation of lysine (K) residues in various histones, specifically K4, K9, K27, and K36 in histone H3 and K20 in histone H4 ; H3K79 is an exception . All members of this family share a highly conserved SET domain , named for three Drosophila melanogaster proteins: S uppressor of variegation 3-9 (Su(var)3-9) (Suv), E nhancer of Zeste (E(z)) and T rithorax (Trx). This domain has approximately 130 amino acids and has been found in all eukaryotic organisms studied so far . Proteins containing the SET domain can also be found in viruses as well as both domains of prokaryotes [4, 5].
Recent studies have revealed that the SET domain (here after referred as SET) genes are important for regulating growth and reproduction processes, such as control of flowering time and embryogenesis in plants . Genome sequencing has uncovered many genes encoding SET-domain proteins; in particular, Arabidopsis SET genes are the best annotated and characterized. For example, the Arabidopsis CURLY LEAF (CLF) gene is required for stable repression of the floral homeotic gene AGAMOUS in leaves and stems . ARABIDOPSIS TRITHORAX 1 (ATX1) functions as an activator of homeotic genes, like Trithorax in animal systems . ARABIDOPSIS TRITHORAX-RELATED PROTEIN 7 (ATXR7) is an H3K4 methylase required for proper expression of the Flowering Locus C (FLC) gene . The Arabidopsis ASH1 HOMOLOG 2 (ASHH2) protein has been suggested to methylate H3K4 and/or H3K36, similar to Drosophila ASH1 and yeast SET2, a H3K36 histone methyltransferase (HMT) . Other SET genes are associated with embryogenesis, including MEDEA (MEA); a maternally inherited loss-of-function mea allele results in embryo abortion and prolonged endosperm production . Recently, ATXR3 is crucial for both sporophyte and gametophyte development and encodes the major enzyme responsible for trimethylation of H3K4 [12, 13].
In plants, at least 47, 33, 31, and 43 SET genes have been identified in Arabidopsis, grape, maize and rice, respectively [14–16]. In both Arabidopsis and rice it has been determined that many SET genes are located in large blocks of related regions derived from whole genome duplication events, indicating that whole genome duplication could be an important contributor to the duplication of SET genes . In addition, different classifications of SET genes were used in different plants. Initially, 37 putative Arabidopsis SET domain proteins were classified into four distinct classes: (I) enhancer of zeste [E(z)] homologs; (II) trithorax (Trx) homologs and related proteins; (III) Ash1 homologs and related proteins; and (IV) suppressor of variegation [Su(var)] homologs and related proteins . In another study, 32 Arabidopsis and 22 maize SET genes were classified into five classes according to phylogenetic relationships and domain organization . More recently, two additional classes (VI and VII) were recognized for SET genes in Arabidopsis, grape, maize, and rice [14–16]. Interestingly, in Arabidopsis several genes in class III, like ATXR3, were shown to be crucial for both sporophyte and gametophyte development [12, 13]. Moreover, Arabidopsis has ten Su(var) homologue (SUVH) genes belonging to Class V, including several that control heterochromatic domains. Loss of function of these genes suppresses gene silencing, whereas overexpression enhances silencing, causing ectopic heterochromatization and significant growth defects in Arabidopsis . Therefore, SET genes in different subfamilies could have diverse functions.
Previous studies of SET genes have focused on annotation and Arabidopsis functional characterizations [7–13, 19]; in addition evolutionary analyses have been limited to herbaceous plants [14–18]. Trees are distinct from herbaceous species in many ways: they have a self-supporting structure, the secondary growth or wood, and a much longer lifespan [20, 21]. The regulatory networks and molecular mechanisms that underlie these unique properties cannot be investigated through the examination of nontree species. Therefore, it is worthwhile to study SET genes in trees, thereby improving our understanding in their functions and the evolution of SET genes. The recently completed genome sequence of the model tree, Populus trichocarpa (hereafter called Populus) , provides a great opportunity to investigate these issues.
Molecular evidence suggests that Arabidopsis and Populus shared their last common ancestor as much as 100 to 120 million years ago . Since then, Arabidopsis and Populus have evolved different life histories, including herbaceous versus arboreal development, annual versus perennial habit, and self-pollination versus cross-pollination strategies [20, 21]. In addition, since they diverged from each other, Populus has experienced whole genome duplication once, whereas Arabidopsis has twice [22, 23]. In plants, evolutionary diversity has been hypothesized to be modulated directly or indirectly by epigenetic regulations . Therefore, the SET gene family, among the most important epigenetic regulators, could be postulated to contribute substantially to the evolutionary innovations in plant diversity.
We conducted a comparative analysis of SET genes from Arabidopsis and Populus to address the key question: how have SET genes evolved in Populus after the divergence of Arabidopsis and Populus. In particular, how did the SET gene family expand and diversify in Populus? In this study, we performed comprehensive analyses of SET genes from Populus, including phylogeny, gene structure, domain architecture, gene duplication and diversification, and expression profiling analyses. Our results provide insight into the function of Populus SET genes and provide a basis for understanding how gene functions, particularly functions involved in the development of trees, have evolved.
Identification of SET genes in Populus
Members of the SET gene family in Populus
Phylogeny and gene structures of Populus SETgenes
The phylogenetic tree topology and the predicted protein domain compositions also allow the grouping of the 106 SET genes in Arabidopsis and Populus into eight subfamilies (named Suv, Atxr5, Ash, Atxr3, E(z), Trx, SMYD and SETD; Figure 1A), generally in accordance with those in other plants [14, 16–18]. Nevertheless, there were some differences from prior classifications [16–18]. One notable difference is that recent classifications placed MEA, CLF and SWN in class I and ATX1, ATX2, ATX3, ATX4, ATX5, ATXR3 and ATXR7 in Class III [14–16], but our results had all of these genes clustering together, forming a monophyletic clade with bootstrap support of 77% (Figure 1A). In addition, each subfamily formed a group with high bootstrap support in the unrooted ML/BI/NJ analysis and may have the same ancestral origin.
In general, members from the same subfamily shared similar exon/intron structures, e.g. intron number and exon length; however, some members of the Suv, SMYD and SETD subfamilies (Figure 1B) had structural differences from other members. In the Suv subfamily, retrotransposition events have been reported [17, 18], which could have contributed to the diversity of the subfamily members. For subfamilies SMYD and SETD, we observed considerable diversity in gene structure and highly divergent sequences among subfamily members, but this diversity and its possible functions have rarely been reported.
Expansion and evolution of the SET gene family in Populus
Estimated dates of the duplication events leading to pairs of SET genes in Populus and Arabidopsis
Number of anchors
Mean K s
SD K s
Estimated time (Mya)
In addition, we calculated the values of synonymous substitutions per synonymous site (K s ) of duplicated gene pairs from PGDD, which we assumed to be correlated with the time of divergence since the genome duplication (Figure 2B). Apparently, the Populus duplicates found in syntenic blocks matched with two WGDs (the first and second peaks, denoted p and γ previously ). There was also a third peak (K s , 3.6-4.2, denoted ε or ζ), which might be due to the ancient angiosperm-wide and/or seed plant-wide WGDs , with possible blurring of the distinction between the two expected peaks due to subsequent K s rate variation. To estimate the divergence time of the 19 duplicated gene pairs, we calculated their syntenic K s values. They could be classified at least into two categories (Table 2). The first category included the 19 gene pairs, whose locations in the syntenic region are shown in Figure 2C and Additional file 2. They had an average K s value between 0.2483-0.2898, corresponding to the first peak (p) in Figure 2B. This duplication event was dated to 13.64-15.93 Mya, corresponding to a recent segmental duplication/WGD event in Populus, approximately 10-20 Mya after the split from the lineage leading to Arabidopsis . The other category included one pair of duplicated blocks (between Atx4/Atx5 and Atx3a/Atx3b); their detailed locations in the syntenic region are shown in Figure 2D and Additional file 2. Interestingly, they had much higher average K s value, 1.5129 (Table 2), corresponding to the second peak. These duplicated blocks could be due to the retention of genes from an ancient WGD event(s) shared by core eudicots . In addition the K s values of between Suvh4a and Suvh4b or Suvh4c were 1.6609 or 1.5428, respectively (Additional file 3: Table S1), suggesting that the duplication of Suvh4a and Suvh4b/c could be due to the WGD shared by the core eudicots. Other pairs of poplar SET genes have K s values ranging from 3.6 and 4.6, consistent with them being from even older WGD duplications, but the K s values are not reliable to assign the specific WGD. Our analysis strong suggests that segmental duplication events, especially those resulting from recent polyploidy events, have contributed to the expansion of the SET domain gene family in Populus.
Domain diversity in Populus SETgene family
K a /K s and divergence analysis of domain architecture and gene structure of duplicate d SET gene pairs in Populus
K a /K s
The terminus diversity
The analysis of the Populus SET genes indicated that one of two recent SET duplicate undergoes domain gain or loss, during a relatively short period of evolutionary time following a recently WGD event. New domain architectures can drive the evolution of organismal complexity ; for example, recombination of domains encoded by genes belonging to the yeast mating pathway had a major influence on phenotype . Therefore, the domain gains and losses in SET genes that occurred 13.64-15.93 Mya might have been a strong force of evolution of Populus complexity. Because SET proteins are important for histone modification and chromatin structure, they can play crucial roles in regulating gene expression during plant development [6, 32]. That their domain architecture has incurred major changes in a short time indicates that epigenetic regulation could be somewhat plastic.
Expression analysis of SET genes in Populus
The expression patterns of SET genes
All showed high expression level in all tissues except Swnb showed relatively low expression in FC and YL
Atx1/2, Atx3b, Atxr3b
Suvr4a/b, Suvh7, Ashh1,
Most are expressed at lower levels and with different patterns than the first subgroup. Setd7 and Suvr4a have low expression levels in all the tissues.
Most had very low expression levels in almost all tissues, but the Clfb and Atx3a expressed at higher levels in certain tissues.
Atxr3a, Clfb, Setd2b
Most had high expression levels in YL, ML and others, but low expression in FC. Suvr1, Atxr1 and Ashh3 have low expression levels in all the tissues.
Ashh3, Ashr2/1, Atxr1/4,
Most genes had low expression levels in all the tissues, but Ashr3, Atx5, Setd6 and Atxr7b showed high expression levels only in FC and MC
Atxr7a/b, Atx4/5, Setd6
Structural divergence of duplicated Populus SETgene pairs via four major scenarios
We have found that the 3'-terminal and 5'-terminal regions (often with part of the coding region) of most duplicated Populus SET gene pairs were highly divergent, particularly the 5' end (Figure 4 and Additional file 5). Further investigation of their sequences revealed that these gene structure differences could have originated from one (or more) of four major scenarios (Figure 4). The first scenario is insertion or deletion (indel) of partial or entire exons (Figure 4-1A and 6-1B). For example, the first exons at the 5' ends of Atxr7a and Atxr7b matched very well with one other except that the exon in Atxr7b lacked three nucleotides at its beginning, indicating an indel event of one codon (Additional file 6 -e.g. 1A). A notable indel case was the loss of an entire exon at the 5' end of the Clfb gene in comparison with Clfa (Additional file 6 -e.g. 1B). Changes in exon length between most duplicated pairs appeared to have resulted from this scenario (Figure 4-1A & 4-1B and Table 3).
The second scenario was the intronization/exonization of a partial or entire exon/intron (Figure 4-2A & 4-1B). The second exons at the 5' ends of Clfa and Clfb were extremely similar in sequence, but Atxr7a was about 300 bp longer at its 3' end; we found that part of a Clfb intron had been exonized in Clfa (Additional file 6 -e.g. 2A). In another interesting case, the second exon in Ashh2a matches an intronic region in Ashh2b, demonstrating intronization of the whole exon (Additional file 6 -e.g. 2B).
The third scenario was the gain of introns, which divide one exon into two or more smaller ones, or the loss of introns, which unite two or more exons into one longer one (Figure 4-3). The exons near the 5' ends of Suvh7 and Suvh1 make a persuasive example (Additional file 6 -e.g. 3). There were two exons, 305 and 1657, at the 5' end of Suvh1, which flanked a 59 bp intron; whereas there was a single exon, 2010, at the 5' end of Suvh7, without an intron.
The last scenario involved an increase or decrease in the UTR at the 5' ends of duplicated pairs genes (Figure 4). Generally, the UTR of one duplicate was longer than the other. For example, Suvh3 had a longer UTR at its 5' end than its corresponding region in Suvh10 (Additional file 6 -e.g. 4). Taken together, Scenarios 1A and 2A alters exon length, whereas Scenarios 1B and 2B and 3 change exon number, or scenario 3 change intron number. In contrast, the 3' ends of the duplicates were not as different as the 5' ends. The most common case was a change in the length of the UTR at the 3' end.
We have performed systematic phylogenetic analysis of Populus SET genes, and determine K s values of duplicates and examined their expression patterns. Our analyses indicate that SET genes have experienced many duplication events during the Populus evolutionary history. The relatively recent duplicates are likely the results of whole-genome duplication, and many show structural divergence, including gain/loss of functional domains. Furthermore, at least some recent paralogs exhibit divergent expression patterns. Therefore, SET genes in Populus provide a case study for evolution of genes following duplication, showing both gene loss and retention with functional divergence at both structural and expression levels.
Genome duplication and evolution of SETgenes
There are 59, 47 and 43 known SET genes in Populus, Arabidopsis and rice, respectively. The Populus genome has experienced at least four rounds of genome duplication: two ancient duplication events prior to the gymnosperm-angiosperm divergence and before the diversification of all extent angiosperms, an intermediate event shared with all core eudicots plants, and a recent event occurring after the divergence of the lineages leading to Populus and Arabidopsis [22, 23, 25]. Our analysis suggested that, of the 19 duplicated pairs of adjacent Populus SET genes on the phylogenetic tree, all resulted from the recent WGD event. Among older duplicates prior to the split of Populus and Arabidopsis, one or two Suv pairs and one Atx pair likely resulted from the core eudicot WGD, and possibly others from older WGDs. Given that at least four rounds of WGD are known in the Populus lineage, many genes loss within the SET gene family appears to have occurred in Populus. The Arabidopsis lineage has experienced two WGD events since its split from the Populus lineage and has lost many of the duplicates . Our investigation of Arabidopsis SET genes revealed five duplicated gene pairs since its divergence from Populus. These five pairs had average K s values between 0.1817-0.2319, with estimated dates between 27.27-28.78 Mya for the duplication, corresponding to a recent WGD in Arabidopsis [22, 23]. Furthermore, soybean has experienced one more WGD event than Populus since its split with the Populus lineage, and it has 25 more SET genes than Populus. Most of the increased soybean SET gene number resulted from the recent WGD (data not shown). Therefore, the expansions in the SET gene family in Arabidopsis, Populus, and soybean could be explained by gene loss and gain after WGD events.
An increase in the number of regulatory genes (i.e. transcriptional and developmental regulators) is one of important factors that facilitated the evolution of more complex developmental systems . Maere et al. estimated that more than 90% of the increases in Arabidopsis regulatory genes were likely caused by genome duplications during the last 150 million years . Our results suggest that WGD could be the main mechanism for the expansion of the SET gene family in Populus, consistent with this idea. To date, many studies have shown that many members of transcriptional factor (TF) gene families survive after WGD events [33, 36, 38], but few papers report on epigenetic regulatory gene families. SET genes are important epigenetic regulatory gene and have been largely retained after WGDs. Epigenetic regulators modulate expression of a large number of functionally related genes. Therefore, our study suggests that more basic regulatory gene families could have evolutionary mechanisms similar to TF genes, which might contribute to the evolution of gene networks and provide insight into chromatin regulatory evolution.
Expression profiles of SET genes and functional diversity of duplicated pairs in Populus
We investigated Populus microarray and EST data and found that most SET genes are expressed relatively widely, suggesting that the Populus SET genes that have survived after WGD events are likely functional. Similarly, almost all Arabidopsis and soybean SET genes were expressed (Additional file 3: Tables S3, S4), again suggesting that these SET genes have functions.
According to the expression patterns of Populus genes and the functions of their Arabidopsis orthologues, we could hypothesize possible functions of these genes in Populus. For example, the orthologue of Swnb in Arabidopsis is SWN, which is involved in H3K27 trimethylation at important floral and shoot developmental genes, including AGAMOUS and SHOOT MERISTEMLESS (STM) . Therefore, Swnb might have a function similar to SWN in regulating Populus flower and shoot development. However, some SET genes were specific to Populus, for example, Suvr4a, Suvr4b, Atx3a, and Atx3b. All of these genes have abundant transcripts in Populus and are expressed at different levels in different tissues. These results suggest that their counterparts in Arabidopsis have been lost and the functions are either not needed in Arabidopsis or performed by other genes.
Interestingly, one of the greatest differences between the Populus SET genes and Arabidopsis SET genes is that there are 19 duplicated Populus SET gene pairs but only five Arabidopsis SET gene pairs. In 12 of the 19 duplicated gene pairs, both copies of were expressed, with two types of expression patterns.
The first type is that one copy was expressed at higher levels than the other in one or more tissues, but the other copy was higher in some other tissue(s) (Figure 6, purple boxes). For example, Ashh2a and Ashh2b corresponded to ASHH2 in Arabidopsis, which negatively regulates shoot branching . The Ashh2a and Ashh2b duplicates in Populus are both expressed at the same stages and could repress shoot branching in Populus. Swna and Swnb corresponded to the Arabidopsis SWN, which exhibits partial functional redundancy with CLF and MEA . Populus is a perennial woody plant with a juvenile-to-mature phase change, so its flowering processes is different from that of Arabidopsis and may require two duplicates with somewhat different functions to regulate these processes.
The second type is that one duplicate was expressed at higher levels than the other in all tissues (Figure 6, black boxes), suggesting that ther former has a stronger function than the latter. The Arabidopsis ATXR3 gene corresponds to the poplar Atxr3a and Atxr3b. ATXR3 mutants are smaller with curly leaves, short roots, early flowering, and female sterility [12, 13]. The closest Populus homologues of ATXR3 may also play important roles in regulating broad developmental processes, consistent with one duplicate having wide-ranging high level expression. Similarly, the fact that Arabidopsis SUVH4 is responsible for the majority of H3K9 dimethylation in heterochromatin and affects the number of floral organs and the expression of PHOSPHOANTHRINILATE ISOMERASE (PAI)  suggests that the Populus homologues Suvh4a and Suvh4b could also regulate multiple developmental processes.
The two different expression patterns in different tissues of the 12 duplicated gene pairs suggest functional diversification and possible function redundancy (or gene silencing), respectively. Many models for the evolution of gene duplications have been proposed in the past nearly four decades . Recently, divergence in expression between duplicates has also been examined for Arabidopsis regulatory genes . The first pattern may support the duplication-degeneration-complementation (DDC) model, in which divergent expression in different tissues allows functional differentiation of the duplicates and finally lead to subfunctionalization or neofunctionalization [43, 44]. On the other hand, the latter pattern might suggest functional reduction for one copy (hypofunctionalization), before the weak copy is completely lost (nonefunctionalization) [41, 45, 46].
We have shown that Populus has gained additional SET genes compared with its common ancestor with Arabidopsis, due to a WGD since the divergence from the lineage leading to Arabidopsis. Those duplicates that have been retained show divergence in both coding regions and expression levels, suggesting that the SET genes might have experienced functional diversification, including possible subfunctionalization. The increased number of SET genes with potentially distinct functions could have supported the evolution of epigenetic gene regulation of a woody perennial that is more complex than that of an herbaceous annual.
Database search for SETgenes
The complete genome and predicted proteomes sequence of Populus was obtained from the JGI [http://genome.jgi-psf.org/Poptr1_1/Poptr1_1.home.html] database . To identify all genes for proteins containing SET domains in Populus, the SET domain PF00856 model from the Pfam database [47, 48] was used to perform a local search of the Populus predicted proteomes using the HMMER program (2.3.2) . To find genes for similar proteins from unannotated genomic regions, the identified proteins sequences (domains) were used as queries for a gene search against the Populus genome sequence using a newly developed software, Phoenix (Protein Homologue Extraction, Sun et al., unpublished). An e-value cutoff value of 1e-5 was used for further analysis. SET genes from Arabidopsis were obtained as described by Zhang et al. .
Multiple sequence alignment, gene structure and protein architecture analyses
Multiple sequence alignment using MUSCLE with default parameters  was performed on SET proteins, and the alignment was subsequently adjusted manually. Gene structure information, including the intron/exon distribution pattern, was obtained by GSDS (Gene Structure Display Server), developed by CBI . Structural motif annotation was performed using the SMART [http://smart.embl-heidelberg.de] and Pfam [http://pfam.sanger.ac.uk] databases.
Genome localization and syntenic analysis of SETgenes
Information on chromosomal location was gathered from the Populus genome browser. MapInspect [http://www.plantbreeding.wur.nl/UK/software_mapinspect.html] was used to map the SET genes onto chromosomes. Syntenic information was collected from the Plant Genome Duplication Database [PGDD: http://chibba.agtec.uga.edu/duplication] [23, 26].
Calculating K s and dating the duplication event
Protein amino acid sequences of all the duplicated gene pairs from Populus were aligned using MUSCLE with default parameters , and the results were used to guide the alignments of DNA coding sequences (CDS) by Pal2Nal . K s and K a , the number of synonymous and nonsynonymous substitutions per site, were determined using the aligned CDS by yn00 in PAML 4.3 .
In dating segmental duplication events, the approximate date of the duplication event was calculated using the mean K s values from T = K s /2λ, where the clock-like rate (λ) for Populus was 9.1 × 10-9 . Similarly, the average K s of all the duplicated SET gene pairs from Populus and Arabidopsis was calculated by the same method, where the clock-like rate (λ) for Arabidopsis was 1.5 × 10-8.
Phylogenetic trees for the aligned SET protein sequences were constructed using NJ, ML, and BI methods. The NJ tree was constructed using MEGA  with the "pairwise deletion" option and "Poisson correction" model. Bootstrap support was estimated from 1000 replicates to evaluate the reliability of internal branches. ML trees were generated using PhyML version 3.0.1, with 100 nonparametric bootstrap replicates and WAG model . MrBayes software  was used to construct BI trees, using the WAG model of evolution, after running for 1,000,000 generations, with four Markov chains sampled every 1000 generations.
Gene expression analysis
To search for SET genes from Populus ESTs, we used the Populus SET CDS as query sequences to search for highly similar ESTs sequences (at least 160 bp and 95% identity) in Populus using the NCBI database. We also analyzed the public microarray dataset of Populus ; we normalized and hierarchically clustered them based on Pearson coefficients with average linkage in the MeV (version 4.8) program . Furthermore, we also got the RNA-seq dataset of soybean .
Million years ago
Plant genome duplication database
Expressed sequence tag
National center for biotechnology Information
- K s :
Synonymous substitutions rate: K a : Non-synonymous substitution rate.
We would like to thank Drs. Fei He, Jinyong Hu, Zhenguo Lin and Zhenguo Zhang for comments on the manuscript and helpful discussions. This work was supported by the National Natural Science Foundation of China (91131007).
- Bannister AJ, Kouzarides T: Regulation of chromatin by histone modifications. Cell Res. 2011, 21: 381-395. 10.1038/cr.2011.22.PubMedPubMed CentralView ArticleGoogle Scholar
- Dillon SC, Zhang X, Trievel RC, Cheng X: The SET-domain protein superfamily: protein lysine methyltransferases. Genome Biol. 2005, 6: 227-10.1186/gb-2005-6-8-227.PubMedPubMed CentralView ArticleGoogle Scholar
- Feng Q, Wang H, Ng HH, Erdjument-Bromage H, Tempst P, Struhl K, Zhang Y: Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain. Curr Biol. 2002, 12: 1052-1058. 10.1016/S0960-9822(02)00901-6.PubMedView ArticleGoogle Scholar
- Mujtaba S, Manzur KL, Gurnon JR, Kang M, Van Etten JL, Zhou MM: Epigenetic transcriptional repression of cellular genes by a viral SET protein. Nat Cell Biol. 2008, 10: 1114-1122. 10.1038/ncb1772.PubMedPubMed CentralView ArticleGoogle Scholar
- Alvarez-Venegas R, Sadder M, Tikhonov A, Avramova Z: Origin of the bacterial SET domain genes: vertical or horizontal?. Mol Biol Evol. 2007, 24: 482-497.PubMedView ArticleGoogle Scholar
- Liu C, Lu F, Cui X, Cao X: Histone methylation in higher plants. Annu Rev Plant Biol. 2010, 61: 395-420. 10.1146/annurev.arplant.043008.091939.PubMedView ArticleGoogle Scholar
- Goodrich J, Puangsomlee P, Martin M, Long D, Meyerowitz EM, Coupland G: A Polycomb-group gene regulates homeotic gene expression in Arabidopsis. Nature. 1997, 386: 44-51. 10.1038/386044a0.PubMedView ArticleGoogle Scholar
- Alvarez-Venegas R, Pien S, Sadder M, Witmer X, Grossniklaus U, Avramova Z: ATX-1, an Arabidopsis homolog of trithorax, activates flower homeotic genes. Curr Biol. 2003, 13: 627-637. 10.1016/S0960-9822(03)00243-4.PubMedView ArticleGoogle Scholar
- Tamada Y, Yun JY, Woo SC, Amasino RM: ARABIDOPSIS TRITHORAX-RELATED7 is required for methylation of lysine 4 of histone H3 and for transcriptional activation of FLOWERING LOCUS C. Plant Cell. 2009, 21: 3257-3269. 10.1105/tpc.109.070060.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhao Z, Yu Y, Meyer D, Wu C, Shen WH: Prevention of early flowering by expression of FLOWERING LOCUS C requires methylation of histone H3 K36. Nat Cell Biol. 2005, 7: 1256-1260.PubMedView ArticleGoogle Scholar
- Grossniklaus U, Vielle-Calzada JP, Hoeppner MA, Gagliano WB: Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis. Science. 1998, 280: 446-450. 10.1126/science.280.5362.446.PubMedView ArticleGoogle Scholar
- Guo L, Yu Y, Law JA, Zhang X: SET DOMAIN GROUP2 is the major histone H3 lysine 4 trimethyltransferase in Arabidopsis. Proc Natl Acad Sci USA. 2010, 107: 18557-18562. 10.1073/pnas.1010478107.PubMedPubMed CentralView ArticleGoogle Scholar
- Berr A, McCallum EJ, Menard R, Meyer D, Fuchs J, Dong A, Shen WH: Arabidopsis SET DOMAIN GROUP2 is required for H3K4 trimethylation and is crucial for both sporophyte and gametophyte development. Plant Cell. 2010, 22: 3232-3248. 10.1105/tpc.110.079962.PubMedPubMed CentralView ArticleGoogle Scholar
- Aquea F, Vega A, Timmermann T, Poupin MJ, Arce-Johnson P: Genome-wide analysis of the SET DOMAIN GROUP family in grapevine. Plant Cell Rep. 2011, 30: 1087-1097. 10.1007/s00299-011-1015-0.PubMedView ArticleGoogle Scholar
- Zhang LS, Ma CR, Ji Q, Wang YF: Genome-wide identification, classification and expression analyses of SET domain gene family in Arabidopsis and rice. Yi Chuan. 2009, 31: 186-198.PubMedView ArticleGoogle Scholar
- Ng DW, Wang T, Chandrasekharan MB, Aramayo R, Kertbundit S, Hall TC: Plant SET domain-containing proteins: structure, function and regulation. Biochim Biophys Acta. 2007, 1769: 316-329.PubMedPubMed CentralView ArticleGoogle Scholar
- Baumbusch LO, Thorstensen T, Krauss V, Fischer A, Naumann K, Assalkhou R, Schulz I, Reuter G, Aalen RB: The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes. Nucleic Acids Res. 2001, 29: 4319-4333. 10.1093/nar/29.21.4319.PubMedPubMed CentralView ArticleGoogle Scholar
- Springer NM, Napoli CA, Selinger DA, Pandey R, Cone KC, Chandler VL, Kaeppler HF, Kaeppler SM: Comparative analysis of SET domain proteins in maize and Arabidopsis reveals multiple duplications preceding the divergence of monocots and dicots. Plant Physiol. 2003, 132: 907-925. 10.1104/pp.102.013722.PubMedPubMed CentralView ArticleGoogle Scholar
- Naumann K, Fischer A, Hofmann I, Krauss V, Phalke S, Irmler K, Hause G, Aurich AC, Dorn R, Jenuwein T, Reuter G: Pivotal role of AtSUVH2 in heterochromatic histone methylation and gene silencing in Arabidopsis. EMBO J. 2005, 24: 1418-1429. 10.1038/sj.emboj.7600604.PubMedPubMed CentralView ArticleGoogle Scholar
- Jansson S, Douglas CJ: Populus: a model system for plant biology. Annu Rev Plant Biol. 2007, 58: 435-458. 10.1146/annurev.arplant.58.032806.103956.PubMedView ArticleGoogle Scholar
- Brunner AM, Busov VB, Strauss SH: Poplar genome sequence: functional genomics in an ecologically dominant plant species. Trends Plant Sci. 2004, 9: 49-56. 10.1016/j.tplants.2003.11.006.PubMedView ArticleGoogle Scholar
- Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, et al: The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science. 2006, 313: 1596-1604. 10.1126/science.1128691.PubMedView ArticleGoogle Scholar
- Tang H, Wang X, Bowers JE, Ming R, Alam M, Paterson AH: Unraveling ancient hexaploidy through multiply-aligned angiosperm gene maps. Genome Res. 2008, 18: 1944-1954. 10.1101/gr.080978.108.PubMedPubMed CentralView ArticleGoogle Scholar
- Feng S, Jacobsen SE: Epigenetic modifications in plants: an evolutionary perspective. Curr Opin Plant Biol. 2011, 14: 179-186. 10.1016/j.pbi.2010.12.002.PubMedPubMed CentralView ArticleGoogle Scholar
- Jiao Y, Wickett NJ, Ayyampalayam S, Chanderbali AS, Landherr L, Ralph PE, Tomsho LP, Hu Y, Liang H, Soltis PS, et al: Ancestral polyploidy in seed plants and angiosperms. Nature. 2011, 473: 97-100. 10.1038/nature09916.PubMedView ArticleGoogle Scholar
- Tang H, Bowers JE, Wang X, Ming R, Alam M, Paterson AH: Synteny and collinearity in plant genomes. Science. 2008, 320: 486-488. 10.1126/science.1153917.PubMedView ArticleGoogle Scholar
- Chothia C, Gough J, Vogel C, Teichmann SA: Evolution of the protein repertoire. Science. 2003, 300: 1701-1703. 10.1126/science.1085371.PubMedView ArticleGoogle Scholar
- Bashton M, Chothia C: The generation of new protein functions by the combination of domains. Structure. 2007, 15: 85-99. 10.1016/j.str.2006.11.009.PubMedView ArticleGoogle Scholar
- Zhu X, Ma H, Chen Z: Phylogenetics and evolution of Su(var)3-9 SET genes in land plants: rapid diversification in structure and function. BMC Evol Biol. 2011, 11: 63-10.1186/1471-2148-11-63.PubMedPubMed CentralView ArticleGoogle Scholar
- Babushok DV, Ostertag EM, Kazazian HH: Current topics in genome evolution: molecular mechanisms of new gene formation. Cell Mol Life Sci. 2007, 64: 542-554. 10.1007/s00018-006-6453-4.PubMedView ArticleGoogle Scholar
- Peisajovich SG, Garbarino JE, Wei P, Lim WA: Rapid diversification of cell signaling phenotypes by modular domain recombination. Science. 2010, 328: 368-372. 10.1126/science.1182376.PubMedPubMed CentralView ArticleGoogle Scholar
- Pontvianne F, Blevins T, Pikaard CS: Arabidopsis histone lysine methyltransferases. Adv Bot Res. 2010, 53: 1-22.PubMedPubMed CentralView ArticleGoogle Scholar
- Wilkins O, Nahal H, Foong J, Provart NJ, Campbell MM: Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiol. 2009, 149: 981-993.PubMedPubMed CentralView ArticleGoogle Scholar
- Xu G, Ma H, Nei M, Kong H: Evolution of F-box genes in plants: different modes of sequence divergence and their relationships with functional diversification. Proc Natl Acad Sci USA. 2009, 106: 835-840. 10.1073/pnas.0812043106.PubMedPubMed CentralView ArticleGoogle Scholar
- Xu G, Guo C, Shan H, Kong H: Divergence of duplicate genes in exon-intron structure. Proc Natl Acad Sci USA. 2012, 109: 1187-1192. 10.1073/pnas.1109047109.PubMedPubMed CentralView ArticleGoogle Scholar
- Proost S, Pattyn P, Gerats T, Van de Peer Y: Journey through the past: 150 million years of plant genome evolution. Plant J. 2011, 66: 58-65. 10.1111/j.1365-313X.2011.04521.x.PubMedView ArticleGoogle Scholar
- Maere S, De Bodt S, Raes J, Casneuf T, Van Montagu M, Kuiper M, Van de Peer Y: Modeling gene and genome duplications in eukaryotes. Proc Natl Acad Sci USA. 2005, 102: 5454-5459. 10.1073/pnas.0501102102.PubMedPubMed CentralView ArticleGoogle Scholar
- Van de Peer Y, Maere S, Meyer A: The evolutionary significance of ancient genome duplications. Nat Rev Genet. 2009, 10: 725-732. 10.1038/nrg2600.PubMedView ArticleGoogle Scholar
- Schubert D, Primavesi L, Bishopp A, Roberts G, Doonan J, Jenuwein T, Goodrich J: Silencing by plant Polycomb-group genes requires dispersed trimethylation of histone H3 at lysine 27. EMBO J. 2006, 25: 4638-4649. 10.1038/sj.emboj.7601311.PubMedPubMed CentralView ArticleGoogle Scholar
- Dong G, Ma DP, Li J: The histone methyltransferase SDG8 regulates shoot branching in Arabidopsis. Biochem Biophys Res Commun. 2008, 373: 659-664. 10.1016/j.bbrc.2008.06.096.PubMedView ArticleGoogle Scholar
- Innan H, Kondrashov F: The evolution of gene duplications: classifying and distinguishing between models. Nat Rev Genet. 2010, 11: 97-108.PubMedView ArticleGoogle Scholar
- Duarte JM, Cui L, Wall PK, Zhang Q, Zhang X, Leebens-Mack J, Ma H, Altman N, dePamphilis CW: Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol Biol Evol. 2006, 23: 469-478.PubMedView ArticleGoogle Scholar
- Force A, Lynch M, Pickett FB, Amores A, Yan YL, Postlethwait J: Preservation of duplicate genes by complementary, degenerative mutations. Genetics. 1999, 151: 1531-1545.PubMedPubMed CentralGoogle Scholar
- Liu SL, Baute GJ, Adams KL: Organ and cell type-specific complementary expression patterns and regulatory neofunctionalization between duplicated genes in Arabidopsis thaliana. Genome Biol Evol. 2011, 3: 1419-1436. 10.1093/gbe/evr114.PubMedPubMed CentralView ArticleGoogle Scholar
- Papp B, Pal C, Hurst LD: Dosage sensitivity and the evolution of gene families in yeast. Nature. 2003, 424: 194-197. 10.1038/nature01771.PubMedView ArticleGoogle Scholar
- Qian W, Liao BY, Chang AY, Zhang J: Maintenance of duplicate genes and their functional redundancy by reduced expression. Trends Genet. 2010, 26: 425-430. 10.1016/j.tig.2010.07.002.PubMedPubMed CentralView ArticleGoogle Scholar
- Finn RD, Tate J, Mistry J, Coggill PC, Sammut SJ, Hotz HR, Ceric G, Forslund K, Eddy SR, Sonnhammer EL, Bateman A: The Pfam protein families database. Nucleic Acids Res. 2008, 36: D281-D288. 10.1093/nar/gkn226.PubMedPubMed CentralView ArticleGoogle Scholar
- Finn RD, Mistry J, Schuster-Bockler B, Griffiths-Jones S, Hollich V, Lassmann T, Moxon S, Marshall M, Khanna A, Durbin R, et al: Pfam: clans, web tools and services. Nucleic Acids Res. 2006, 34: D247-D251. 10.1093/nar/gkj149.PubMedPubMed CentralView ArticleGoogle Scholar
- Eddy SR: Profile hidden Markov models. Bioinformatics. 1998, 14: 755-763. 10.1093/bioinformatics/14.9.755.PubMedView ArticleGoogle Scholar
- Edgar RC: MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics. 2004, 5: 113-10.1186/1471-2105-5-113.PubMedPubMed CentralView ArticleGoogle Scholar
- Guo AY, Zhu QH, Chen X, Luo JC: GSDS: a gene structure display server. Yi Chuan. 2007, 29: 1023-1026.PubMedView ArticleGoogle Scholar
- Suyama M, Torrents D, Bork P: PAL2NAL: robust conversion of protein sequence alignments into the corresponding codon alignments. Nucleic Acids Res. 2006, 34: W609-W612. 10.1093/nar/gkl315.PubMedPubMed CentralView ArticleGoogle Scholar
- Yang Z: PAML 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol. 2007, 24: 1586-1591. 10.1093/molbev/msm088.PubMedView ArticleGoogle Scholar
- Lynch M, Conery JS: The evolutionary fate and consequences of duplicate genes. Science. 2000, 290: 1151-1155. 10.1126/science.290.5494.1151.PubMedView ArticleGoogle Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.PubMedView ArticleGoogle Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704. 10.1080/10635150390235520.PubMedView ArticleGoogle Scholar
- Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19: 1572-1574. 10.1093/bioinformatics/btg180.PubMedView ArticleGoogle Scholar
- Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, Howe EA, Li J, Thiagarajan M, White JA, Quackenbush J: TM4 microarray software suite. Methods Enzymol. 2006, 411: 134-193.PubMedView ArticleGoogle Scholar
- Severin AJ, Woody JL, Bolon YT, Joseph B, Diers BW, Farmer AD, Muehlbauer GJ, Nelson RT, Grant D, Specht JE, et al: RNA-Seq Atlas of Glycine max: a guide to the soybean transcriptome. BMC Plant Biol. 2010, 10: 160-10.1186/1471-2229-10-160.PubMedPubMed CentralView ArticleGoogle Scholar
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