Diversification and reproductive isolation: cryptic species in the only New World high-duty cycle bat, Pteronotus parnellii
- Elizabeth L Clare†1, 2Email author,
- Amanda M Adams†3,
- Aline Z Maya-Simões3, 5,
- Judith L Eger4,
- Paul DN Hebert1 and
- M Brock Fenton3
© Clare et al.; licensee BioMed Central Ltd. 2013
Received: 10 July 2012
Accepted: 17 January 2013
Published: 29 January 2013
Molecular techniques are increasingly employed to recognize the presence of cryptic species, even among commonly observed taxa. Previous studies have demonstrated that bats using high-duty cycle echolocation may be more likely to speciate quickly. Pteronotus parnellii is a widespread Neotropical bat and the only New World species to use high-duty cycle echolocation, a trait otherwise restricted to Old World taxa. Here we analyze morphological and acoustic variation and genetic divergence at the mitochondrial COI gene, the 7th intron region of the y-linked Dby gene and the nuclear recombination-activating gene 2, and provide extensive evidence that P. parnellii is actually a cryptic species complex.
Central American populations form a single species while three additional species exist in northern South America: one in Venezuela, Trinidad and western Guyana and two occupying sympatric ranges in Guyana and Suriname. Reproductive isolation appears nearly complete (only one potential hybrid individual found). The complex likely arose within the last ~6 million years with all taxa diverging quickly within the last ~1-2 million years, following a pattern consistent with the geological history of Central and northern South America. Significant variation in cranial measures and forearm length exists between three of the four groups, although no individual morphological character can discriminate these in the field. Acoustic analysis reveals small differences (5–10 kHz) in echolocation calls between allopatric cryptic taxa that are unlikely to provide access to different prey resources but are consistent with divergence by drift in allopatric species or through selection for social recognition.
This unique approach, considering morphological, acoustic and multi-locus genetic information inherited maternally, paternally and bi-parentally, provides strong support to conclusions about the cessation of gene flow and degree of reproductive isolation of these cryptic species.
KeywordsCryptic species DNA barcoding Systematics Bats Biodiversity Speciation Pteronotus mesoamericanus
Mammal species diversity in the Neotropics
Central and northern South America are frequently cited as hotspots for speciation due to complex biological and geological processes in this area. The rise of the Central American land bridge some 3 million years ago caused a sudden mixing of previously isolated terrestrial species in the Great American Interchange while simultaneously isolating marine populations on the Atlantic and Pacific sides . The rise of the Andes Mountains, including the Eastern Cordillera ending some 2–3 million years ago , served to isolate previously contiguous terrestrial populations, particularly those with low dispersal abilities. Given these two geological events, it is not surprising that this geographical area has been the focus of many phylogeographic and taxonomic investigations in a wide variety of taxa. Species with large body size tend to have greater dispersal ability  and volant species may be particularly capable of overcoming dispersal barriers , likely intermixing populations from Central and South America more easily. Theoretically, increased dispersal ability may result in more homogenized populations  reducing population structure and thus speciation. Despite this, the only volant mammals, bats, are frequently found to have significant population divisions, subspecies, and sister-species relationships between Central and South American taxa [6–9].
Avise and Walker  hypothesized that half of all vertebrates remain undescribed. This level of cryptic diversity might be expected among groups that have received little taxonomic scrutiny, such as invertebrates (e.g. [11, 12]), but the presence of new species among large, charismatic, well-studied taxa is less expected. Despite this, an average of 223 mammalian species have been described each decade since the beginning of Linnaean taxonomy and the rate of discovery has increased recently, particularly in areas of high endemism . The contemporary discovery of new species rarely reflects the discovery of novel morphological forms or the rediscovery of species thought extinct (though see ). Instead, new species are generally uncovered during reassessments of existing taxa using multiple lines of new evidence (behaviour, ecology, molecular markers), in conjunction with traditional morphological analysis. While morphological differences may be subtle and thus easily overlooked, high genetic divergences within species often indicate cryptic diversity and can direct taxonomic analysis. Evidence of undescribed species has been found even among well-studied mammals, such as orangutans , warthogs , giraffes , muntjacs , baleen whales , beaked whales  and elephants  and further cases are to be expected among less known taxa. Among mammals, bats are the second largest order with more than 1100 described species . They are an obvious target for the discovery of new species as their cryptic behaviour (e.g. nocturnal and active high in the air) makes them difficult to study in the wild . Genetic analysis has detected cryptic species among the European bat genus Pipistrellus, the widely distributed genera Plecotus and Myotis and overlooked species appear common in both Southeast Asia  and the Neotropics [7–9, 25, 26].
Rapid speciation in bats with high-duty cycle echolocation
The majority of bats use acoustics, mainly echolocation, as a primary means of orientation, prey detection, and social recognition. Two forms of echolocation exist among bats: low-duty cycle echolocation entails separating pulse and echo in time, while high-duty cycle echolocation employs Doppler shift compensation to separate pulse and echo in frequency. High-duty cycle echolocators have an “acoustic fovea” which allows them to resolve fine differences in frequency [27–29]. High-duty cycle echolocation occurs in ~120 species in the Old World families Rhinolophidae and Hipposideridae . Only one species in the New World, Pteronotus parnellii (family Mormoopidae), has evolved high-duty cycle echolocation with the most energy of the call in the second harmonic, ~61.5 kHz , with harmonics at ~30 kHz intervals.
Among echolocating bats, acoustic traits may diverge by drift in allopatric populations (see discussion in ); however, it has been argued that selection for non-interference between inter-population calls in sympatric zones may also drive speciation  through local adaptation . Four hypotheses for speciation and echolocation have been proposed. First, call divergence can occur through disruptive ecological selection on frequency leading to prey specialization and gradually to reproductive isolation  with speciation as a by-product of acoustic resource partitioning. High frequencies are more effective at detecting small insects and orienting in cluttered environments while low frequency calls provide more details on large insects and over longer distances, though reasonably large differences in emission frequency are required for functional differences in target identification [34, 35] due to the relationship between wavelength and target detection of insect sizes (see  for complete details). As a result of this selective pressure we expect large acoustic variation between competing species in order for them to access different sized prey (e.g. 10 kHz difference in frequency translates into only a small difference in target strength detection; see [33, 35]). Second, selection for non-interference in acoustic signals between populations may cause similar patterns of acoustic divergence. In two morphs of Hipposideros bicolor, Kingston et al. noted substantial acoustic divergence but not enough (<10 kHz) for significant resource partitioning. Kingston et al. suggest that selection has acted against signal interference yielding character displacement due to social interactions. Under this hypothesis, we would predict small but consistent variation in echolocation frequency (<10 kHz). Third, acoustic divergence can cause both resource and social isolation simultaneously if populations specialize on a lesser-used harmonic of their fundamental. For example, species in the high-duty cycle Rhinolophus philippinensis complex use alternate harmonics consistent with a hypothesized switch via “harmonic hopping” creating an almost instantaneous method of reproductive isolation as the taxa become unable to detect each others’ calls . At the same time, switching harmonics significantly changes the insect prey detection parameters to an almost non-overlapping resource use or “ecological discontinuity” . In the Rhinolophus case, divergence between acoustic morphs may lead to both assortative mating and selection for divergent resource preferences. Most interestingly, Kingston and Rossiter  note that all known echolocation in the clade occurs at a harmonic of the fundamental frequency of the large R. philippinensis morph suggesting rapid radiation via this mechanism. Under this hypothesis, acoustic divergence would be large (>10 kHz) but in specific harmonic intervals of the fundamental frequency of the ancestral population. Finally, allopatric populations may experience acoustic variation due to drift which may be reinforced during secondary contact. Similar to selection for non-interference, these divergences are expected to be small.
While these systems may act in low-duty cycle echolocation, Kingston et al. argued that divergence in acoustic characters is more likely to cause fast reproductive isolation in high-duty cycle echolocation where selection for mate recognition within phonic groups is reinforced by postnatal tuning of the auditory fovea forming a positive feedback loop. Divergence in high-duty cycle echolocation may be selectively stronger since tuning of the constant frequency component and the auditory fovea for insect flutter detection constrains the use of acoustics for social purposes.
Molecular Diversity in Pteronotus parnellii
Genetic analysis has helped reveal many bat species complexes (e.g. [7, 9, 23, 33, 36, 37]). Pteronotus parnellii was described from Jamaica  but is widely distributed in Mexico, Central America, the Antilles, the Guyana Shield and the Amazon . Molecular analysis has revealed substantial sequence divergence at mitochondrial loci [8, 25, 38, 39] though, to date, no multi-gene analysis of this variation has been conducted.
In this study we examine specimens of P. parnellii from ten countries in Central and northern South America to assess morphological, genetic, and acoustic divergences within this species. Following Clare , we test whether P. parnellii is actually a complex of undescribed species by comparing patterns of divergence in maternally inherited DNA and the paternally inherited 7th intron of the Dby region (Ddx3y, DEAD box RNA helicase Y) on the y-chromosome . In addition, we acquired exonic sequences from the 5’ half of the nuclear recombination-activating gene 2 (RAG2) to support phylogenetic reconstructions. We predict that 1) patterns of mitochondrial divergence will be supported by divergence at non-mitochondrial loci, 2) genetic divergence corresponds to subtle morphological differences between these unrecognized species and, 3) acoustic divergences have led to identifiable phonic groups.
Given the geological barriers (such as the Andes) and isolated populations in the Antilles, we anticipate that cryptic species may exist with both allopatric and sympatric distributions and their origin may also have occurred under either of these scenarios. As such, we compare the acoustic patterns of individuals from this range and look for patterns corresponding to the competing hypotheses for acoustic divergence in allopatry and sympatry: 1) if acoustic divergence is primarily for resource partitioning in sympatry, then signal divergence between phonic groups will be large (>10 kHz), 2) if acoustic divergence has occurred through drift in allopatry or selection for non-interference between phonic groups without causing changes in resource use, variation will be significant but small (<10 kHz), and 3) if divergence follows the “harmonic hopping” model of sympatric divergence, then constant frequency components between phonic groups will occur with the most energy at a harmonic of the frequency of ~61.5 kHz attributed to this species.
Acquisition of samples
We sampled tissue from 343 vouchered specimens of P. parnellii at the Royal Ontario Museum (ROM) originating from Mexico, Guatemala, El Salvador, Panama, Venezuela, Guyana and Suriname. We analyzed an additional 106 wing biopsies from un-vouchered individuals caught in Costa Rica and Belize using harp traps and mist nets and from a group of captive individuals originating in Trinidad. Descriptions of all specimens (sampling location, GPS coordinates of collection, voucher number, etc.) are available within the “Bats of the Neotropics” project in the Barcode of Life Data Systems (BOLD, http://www.barcodinglife.org). Records for un-vouchered specimens are contained on BOLD within the projects “Bats of Belize,” “Bats of Costa Rica,” and “Pteronotus parnellii from the Islands.” GenBank and BOLD accession numbers for all sequences, along with sequence alignments, are found in the Additional files 1, 2, 3, 4. All live animals used during the course of this study were caught and handled following the guidelines of the Canadian Council on Animal Care (CCAC – species-specific recommendations on bats) under approval from the Animal Care Committee, University of Guelph (#08R132) and MINAET-ACG permit (FOI-00-004) (Costa Rica). All other tissues and acoustic recordings were acquired from existing collections.
Acquisition and analysis of sequences
We used standard protocols for DNA extraction, amplification, and sequencing for a 657 bp segment near the 5′-terminus of the mitochondrial COI gene  for all individuals. We generated sequences from the Dby 7th intron region of the y-chromosome from DNA extracts of 80 male specimens using the primers in Lim et al.. For 70 male individuals we recovered 767 bp from the 5’ end of the nuclear exonic region of the RAG2 gene (region F1B to R1) using the primers and regions described by Baker et al.. All PCR and sequencing primers are given in Additional file 5 and references therein. We edited COI sequences using SeqScape v.2.1.1 (Applied Biosystems) and Dby 7th intron and the RAG2 region sequences in Sequencher v.4.5 (Gene Codes), and manually aligned all sequences in BioEdit v.7.0.9 (Ibis BioSciences).
We constructed a 95% confidence limit haplotype network of all COI sequences using statistical parsimony in TCS v.1.13 . Following Clare , in all further analyses we define putative cryptic species or “groups” based on the presence of independent (unconnected) COI networks at the 95% confidence level.
We selected appropriate models of sequence evolution in MODELTEST  executed in Phylemon v.2.0  using the Akaike information criterion (AIC) for each set of sequences (COI, Dby 7th intron and RAG2 region). We reduced all datasets to unique haplotypes for phylogenetic reconstruction. For COI sequences and a combined COI + Dby 7th intron + RAG2 sequence dataset we constructed a maximum likelihood phylogeny using PhyML v.3.0  as implemented by the ATGC Montpellier Bioinformatics Platform (http://www.atgc-montpellier.fr/phyml/) using the best fit model of sequence evolution. Branch support was calculated using the non-parametric Shimodaira-Hasegawa-like (SH-like) approximate likelihood ratio test (aLRT) . For the same datasets we constructed a Bayesian phylogeny partitioned by gene and codon position using alternate models of sequence evolution in MrBayes v.3.1.2 . The analyses were performed for 1,000,000 generations for every 10 specimens in the analysis (e.g. for 70 specimens + 3 outgroups in the concatenated tree, runs ran for 7.3 million generations to reach stationarity) and sampled every 50 generations with a burnin of 10,000 generations using multiple outgroups (Noctilio albiventris and Saccopteryx bilineata from South America and Rousettus aegyptiacus from the Old World). Convergence and stationarity were compared between multiple runs. We also evaluated species trees against this concatenated gene tree with the same parameters using the *BEAST option in BEAST v.1.7.4  including input files generated in BEAUTi v.1.7.4 .
Estimates of divergence time
We estimated divergence times among genetic groups with RAG2 and COI sequences using Bayesian MCMC, executed in BEAST (as above). Since no estimates of COI mutation rate are available for bats, we did not use a fixed substitution rate but estimated minimum and maximum divergence times using two substitution models, 2% and 5% per million years, based on estimates for cyt b in small-bodied mammals . Similar estimates of 2.6% for phyllostomid bats , 2.3–5% in Carollia, and 4% from fossil calibrations  have been suggested. We used a fixed clock of 0.194% per million years for the RAG2 sequences . The nucleotide substitution model was the same as that used for phylogenetic analysis. Branching structure of the groups was fixed based on the topology supported by the multi-gene reconstruction. The chain ran for 10,000,000 generations using the 28 unique haplotypes + outgroups with a burnin of 10,000. We estimated the mean and 95% confidence interval (CI) of the divergence times using Tracer v.1.4.1  and summarized the trees using TreeAnnotator v.1.4.8 . We visualized the trees with FigTree v.1.2.1 (A. Rambaut 2009 http://tree.bio.ed.ac.uk/software/figtree).
Analysis of morphology
Analysis of acoustic variation
We acquired echolocation calls recorded during passive monitoring using Avisoft-Bioacoustic CMPA/CM16 condenser ultrasound microphones (Avisoft Bioacoustic 2006) at sites in Jamaica (the type locality of this species), Belize, Costa Rica, southern Guyana (B Lim, pers. comm.), and Trinidad (H Goerlitz pers. comm.). The acoustic records were collected at the same location as the site of genetic sampling but cannot be traced to specific specimens. We analyzed calls using callViewer18 , recording the constant frequency component of each call. We analyzed all calls in a pass (>5 calls), with each pass being considered a separate individual.
We compared forearm length among the genetic groupings with a Kruskal-Wallis test in R 2.13.1 and conducted a pair-wise post-hoc test using kruskalmc (pgirmess package) . All remaining statistical analysis was done using PASW 18 . Because cranial measures are internally correlated, we performed a principal components analysis (PCA) with ten of the skull measurements. We excluded the measure of post-palatal extension from analysis as it is nearly invariant between individuals from all groups. We then used discriminant function analyses (DFA) with the principal component (PC) scores generated from the PCA to classify individuals and an ANCOVA to examine the relationship between sex, latitude and PC scores. Since the groups are distributed with rough association to latitude, we regressed the PC scores against latitude and used the residuals in a DFA to see if there is still a difference among groups above the latitudinal cline.
We compared the constant frequency of the second harmonic among groups with an ANOVA and Tukey’s post-hoc test. Only the constant frequency of the second harmonic was analyzed because of the high correlation between harmonics. For all locations, the second harmonic had the highest intensity and is thus most consistently present and quantifiable. We used a DFA to see how well bats could be classified to their genetic grouping and to the five acoustically sampled locations based on the constant frequency component of their echolocation.
Models of sequence evolution and phylogenetic reconstruction
Mean pairwise COI sequence divergence estimated using the Kimura-2 parameter model of sequence divergence within and among 4 recognized groups
Congruence in mitochondrial, y-chromosome and RAG2 sequences
Among COI sequences we identified 124 variable positions yielding 47 unique haplotypes. In the Dby 7th intron region we identified 13 variable positions and a 15 bp indel starting at the 214th bp, which together yielded three unique haplotypes. In the RAG2 region we identified 12 variable positions yielding ten unique haplotypes.
Estimates of divergence time
Analysis of morphology
Eleven cranial measures from Pteronotus parnellii used in this study
Breadth Of braincase
Alveolar length maxillary toothrow
Alveolar length mandibular toothrow
Breadth of post-palatal extension
Depth of braincase
Group 1 N=27
Group 2 N=5
Group 3 N=60
Group 4 N=74
Females had generally smaller skulls (PC1: F2,161 = 10.12, p<0.001) that were also shorter and wider (PC2: F2,159 = 4.75, p=0.009). There were no interactions between sex and either latitude or group, which confirms that the pattern of sexual dimorphism was consistent among locations. For PC1 there was a significant interaction between group and latitude (F3,155 = 15.308, p<0.001). There were no significant interactions among sex, group and latitude in PC2. There was no effect of latitude on PC2 (F1,159 = 1.68, p = 0.197), but PC2 did vary significantly among groups (F3,159 = 24.66, p<0.001) where Group 3 differed from the other three groups.
Divergence in acoustic recordings
Mean constant frequency (kHz±SD) for the second harmonic for Pteronotus parnellii in a) genetic groups and in b) the five sampled locations
Mean constant frequency (kHz)
a) By Genetic Group
Group 3 or 4*
b) By Sample Location
Jamaica (Type location)
In this study we have tested the hypothesis that P. parnellii consists of multiple undescribed species by exploring genetic, morphological, and acoustic divergences among four identified mitochondrial lineages. We demonstrate that intraspecific lineages identified with mitochondrial DNA are supported by fixed characters in a y-linked intron and the nuclear recombination activating gene 2 as well as by significant morphological differences. Acoustic variation is also evident within this complex, which corresponds to both genetic groups and geographical locations. Further, acoustic divergence is subtle, between 2.5 and 11 kHz, translating into wavelength differences of only 0.23–1.12 mm which are not likely to provide functional differences in resource use. This suggests that acoustic signals have diverged primarily through drift in allopatric populations or through selection for non-interference in sympatric groups rather than ecological selection for different prey sizes.
A cryptic species complex
Mayer and von Helversen  showed that classification of bats based on morphological characteristics does not always correlate with mtDNA divergence. A similar pattern appears to be present here, where P. parnellii on the mainland harbours at least four genetically distinct taxa. These taxa appear to be morphologically cryptic without obvious characters which differentiate them in the field, though statistically significant morphological differences, acoustic variation, and genetic divergence support their existence. The groups do not appear to match the distributions reported for sub-species  (though see below). We have not included discrete character states in our morphological analysis (fur colour, banding pattern, etc.) as these are harder to quantify, but they may represent useful field characters for further investigation.
While any single uni-parentally inherited molecular marker has limitations (e.g. inability to assess hybridization), mtDNA can be a powerful tool for hypothesis generation in taxonomic research. In mammals, cytochrome b has historically been employed for similar analyses (e.g. [7, 36, 59]) though COI is favoured by DNA barcoding campaigns and has been employed extensively in Neotropical bats [8, 9, 25, 26] allowing for easy comparison of sequence divergence (in particular see Clare et al. for a review of COI sequence divergence in >9,000 individuals of 165 Neotropical bat species). Mitochondrial regions are frequently paired with one or more non-mitochondrial loci to test these hypotheses. Y-chromosome regions are ideal for comparison to mtDNA because they are fast evolving and non-recombining, but provide an exclusively paternal measure of gene flow. Particularly when male-biased gene flow is suspected (as is frequent in mammals; e.g. ) y-chromosome DNA can provide a rigorous test of the patterns observed in mtDNA (see ). Nuclear genes are also frequently used in phylogenetic analyses, but many are too slowly evolving for species level diagnosis, particularly when species are young. In addition, nuclear genes are bi-parentally inherited, raising problems of heterozygosity and recombination from multiple alleles. Nuclear genes can effectively support mitochondrial genes in phylogenetic reconstructions. In this case, the RAG2 region appears to provide limited but reliable support for a split between Groups (1+2) and (3+4) (Figure 5) and thus supports a phylogenetic topology which unites these groups (Figure 4). It should be noted that both the RAG2 and COI regions analyzed are protein-coding and thus subject to selection, however they have very different functions (immune system and electron transport chain respectively) resulting in different selection profiles. Additionally, neither region is known to be linked to any morphological trait, therefore they act as relatively independent markers.
In our analysis, all three genes provide evidence for a split between Groups (1+2) and Groups (3+4). The split between Groups 1 and 2 is not observed in the other two gene regions so we cannot evaluate the probability of male-biased gene flow but, as they are allopatric and acoustically distinct, the probability of hybridization is low. These two meet the criteria for the genetic species concept (GSC) advocated for mammals . The GSC evaluates species based on the Bateson-Dobzhansky-Muller model and permits small amounts of gene flow if the genetic groups are on independent evolutionary trajectories . Unlike the biological species concept, the GSC is applicable to allopatric populations and provides a framework for evaluating these groups. Finally, the y-chromosome supports the split between Groups 3 and 4. We were able to evaluate both mtDNA and the y-linked regions from these sympatric males and found little evidence of hybridization (n=1). This is lower than previously reported in other bat taxa, for example, Hoffman et al. proposed cryptic species in Uroderma bilobatum and found two hybrids in 46 individuals and one potential F1, and in the European bats Myotis myotis and M. blythii introgression may be measured in 25% of individuals . The low level of hybridization measured here is acceptable under the GSC and is also generally permitted under a relaxed biological species concept.
Though the Dby 7th intron region has been amplified and sequenced in a wide variety of taxa [9, 40], some caution is required in interpreting the data. While the region is fast evolving it does not appear to evolve as fast as mitochondrial DNA. Additionally, like mtDNA, it may be subject to reduced variability from selective sweeps. An homologous region has been identified on the X-chromosome in some species though it is substantially divergent . We saw no evidence of co-amplification from the male X-chromosome and it is likely that it is either absent in P. parnellii or that these primers preferentially bind to the target area, though no rigorous testing has been done to our knowledge. The family Mormoopidae contains two genera: Mormoops, with three extant species, and Pteronotus, with seven extant species [63, 64]. Of these, most are confined to Central America and the Antilles. P. parnellii appears to be the oldest lineage in the genus, and perhaps the family . While all other species of Pteronotus may have a Central American origin  the origin of P. parnellii is still unclear though our phylogeny suggests a South American origin (see below).
We employed fixed clock estimates of 2% and 5% to exploit the upper and lower calibration points commonly used for bat divergence time calculations in mammal mtDNA. More precise calibrations have been used in other bat taxa, but these are taxonomically specific and estimated for cytochrome b. Given that COI is thought to evolve slightly slower than cytochrome b, using exact calibrations calculated for cytochrome b in bats would be inappropriate and we therefore employed minimum and maximum range bracketing. Previous calibrations have not been made for P. parnellii or COI in bats, thus our divergence rate calculations are meant to approximate the upper and lower estimates of divergence time in these analyses and to minimize errors in the divergence time estimates if any specific calibration point was used. The similar estimates from the RAG2 region suggest these are appropriate boundaries.
Using fixed clock estimates (Figure 4), Groups (1+2) and (3+4) diverged from a common ancestor 2.5- 6.1 MYBP during land bridge formation which ended ~3 MYBP  and a similar estimate was recovered from the RAG2 region. Groups 1, 2, 3 and 4 all radiated during or after the rise of the Eastern Cordillera 2–3 MYBP . The most likely biogeographic scenario is that these groups have a South American origin as suggested by the phylogeny (a single invasion of Central America in the ancestor of Group 1) but invaded Central America during the great American interchange. The rise of the Eastern Cordillera effectively isolated the Central American population, giving rise to Group 1 in allopatry. However, our observations should be treated as preliminary. Additional samples from the Antilles region will be required to establish this definitively and to determine whether the invasion may have involved island hoping. Groups 2, 3 and 4 all exist within the Guyana Shield region which is one of the South American cratons [66, 67]. While no obvious geological event correlates to the divergence, specimens in Group 2 are associated with higher elevations within the Guyana Highlands  though sampling here was minimal and the presence of the complex in Trinidad suggests more habitat variability. It is plausible that Group 2 has re-invaded South America either via the Antilles or a dispersal event over the Andes (additional sampling in Columbia, Venezuela, and the Antilles may resolve this). The divergence of Group 3 and 4 from a common ancestor occurred between 1.1 and 2.7 MYBP and both are associated with sympatric ranges at lower elevations within the Guyana shield region. While there is no contemporary pattern of geology or climatology that can provide a mechanism for this divergence, the region has experienced multiple climate shifts and continual forest change so divergence may have involved a past habitat restriction and vicariance event. Our sampling concentrates on the Central American and northern South American portions of the P. parnellii range and includes individuals from ten countries. We have not sampled individuals in the southern extent of their range, particularly Bolivia, Peru, and Central Brazil, or populations in the Caribbean which includes distributions of four additional subspecies . Our intention was to sample areas encompassing all mainland subspecies however our results suggest that the distribution of these subspecies does not correspond with existing molecular divergences (see below). Additional sampling in these ranges will almost certainly uncover additional cryptic diversity and may help clarify the existence and ranges of these subspecies, their systematic status, and the origin and divergence of the complex.
Systematic considerations in the complex
The taxonomic status of P. parnellii is exceedingly complex. Herd  recognized nine subspecies; P. p. parnellii, P. p. gonavensis, P. p. portoricensis and P.p. pusillus in the Antilles and P. p. mexicanus, P. p. mesoamericanus, P. p. paraguanensis, P. p. fuscus and P.p. rubiginosus on the mainland. Of these, P. p. paraguanensis was recently elevated to a species in Venezuela . The most important systematic question regarding the groups recognized in this study is whether any may be considered P. parnellii sensu stricto. The type location for the taxon is Jamaica and is thought to encompass the subspecies P. p. parnellii. While we have been unable to include a genetic sample from this location in our analysis, the Jamaican population is acoustically distinct. We have some limited morphological data from Jamaica (not included), which demonstrates that this population is comprised of much smaller individuals than those on the mainland. For example, the forearm measures in Jamaica are 53 mm±1.26 SD (range 43–55, n=58, S. Koenig pers. comm.) which is non-overlapping with any mainland population we have examined. Additionally, a comparison of our cranial measurements with those presented for subspecies in Smith  indicate Group 1 best matches the measurements for P. p. mesoamericanus with little overlap among our measurements and those for P. p. parnellii. From this, we conclude that Group 1 corresponds with P. p. mesoamericanus and an elevation of that name would be appropriate. The individuals in our remaining groups are even larger thus none corresponds morphologically to P. p. parnellii. Suggesting appropriate names for the remaining three groups is more difficult as their distribution does not correspond with subspecies distributions suggested by Herd . The remaining Antillean subspecies are even smaller than P. p. parnellii and their distributions disjunct, therefore these names are unlikely to be appropriate; likewise the distribution of P. p. mexicanus precludes a likely correspondence with Groups 2, 3 or 4. Of the remaining subspecies, P. p. paraguanensis (hereafter referred to by its species status), P. p. fuscus, and P. p. rubiginosus, distributions from Herd  do not correspond with our analysis. In Venezuela, Gutierrez and Molinari  report that P. paraguanensis is significantly smaller than either P. p. fuscus or P. p rubiginosus, with P. p. rubiginosus the largest; however Gutierrez and Molinari  agreed with Herd  that only P. p. rubiginosus is found east of the Rio Orinoco suggesting that these subspecies do not correspond with any specific group identified here unless the distributions are much larger than previously reported. Thus, while we are confident that none of our genetic groups can rightly be called P. parnellii, we cannot, at this stage, suggest what names would be appropriate for Group 2, 3 and 4. In the interim, we suggest they be referred to as Pteronotus species 2, 3 and 4 until more appropriate binomials can be established. Barring contradictory evidence, we further conclude that P. parnellii be considered a taxon endemic to the Antilles. The most appropriate future analysis will be a molecular comparison between type material for these subspecies and the groups identified here with additional sampling in the Amazon and Antilles. See also Dávalos  for a discussion of diversification in this family.
Divergence of morphological characters
Though forearm and cranium measurements are both correlated with overall size, we have treated them as independent in our statistical analysis as they have different applications. Cranial characters may only be measured accurately in extracted skulls (e.g. a museum collection), while forearm measurements can be easily obtained from live animals. As such, forearm length is frequently employed as a quick taxonomic field character. However, due to the high overlap in the forearm measurements of the groups, this would not be a reliable measure to separate these groups in the field. Our analysis indicates that the cranial characters advocated by Smith  for the family can differentiate our groups with reasonable accuracy and may act as a useful tool for further investigation of subspecies throughout the entire range, though some overlap does exist thus molecular evidence is a more reliable definitive character. While the four groups can be largely discriminated by DFA (87% successful), there is a significant effect of latitude on the main principal component (PC1-size). Once we corrected for the latitudinal cline, DFA continued to show the groups separating out based on cranial morphological characters. Differences between the two sympatric groups, 3 and 4, became more apparent, while divergence between Groups 1 and 2 was not as obvious after correction (Figure 7). This suggests that the morphological differences of Groups 3 and 4 to all other groups are not solely driven by the latitudinal cline, but this is less clear with Groups 1 and 2.
There was a strong effect of sex on both the size (PC1) and shape (PC2) of the skull suggesting some sexual dimorphism in all locations. The shape of the skull (PC2) did not show a latitudinal effect but did vary between Group 3 and all other groups, further suggesting that there are morphological differences among the groups that are not driven by latitude.
Acoustic divergences within and between groups
Our analysis suggests that there is significant acoustic variation in these groups, creating distinct calls which can differentiate among Groups 1, 2 and 3/4. There is a steady decrease in the frequency of the call with decreasing latitude which also corresponds to an increase in the size of many morphological components.
Interestingly, enough variation is present to further distinguish between some regions within Group 1. We cannot conclude whether there are acoustic differences between the sympatric Groups 3 and 4, as we are limited by relatively few recordings from the region and an inability to separate free-flying bats in field recordings. Further sampling will be required in combination with molecular analysis to determine whether these two groups share an identical echolocation call or whether an additional acoustic pattern exists in this region. If it does, it could prove a powerful tool in identifying the two groups in the field and this is a clear goal for future field studies (sound files available from the authors on request). In addition to echolocation calls, it would be extremely valuable to examine the role of non-echolocation vocalizations among these groups. The role of “social calls” in intra- and interspecific recognition has not been well documented; however the degree of variation in social call repertoire is extensive and may play an important role in mate recognition.
The acoustic variation observed here is not consistent with the pattern of harmonic hopping observed by Kingston and Rossiter  in the R. philippinensis complex, but is similar to divergence patterns in the H. bicolor complex . Individuals are unlikely to recognize the calls of other groups if they come into auditory contact. Furthermore, the divergence between calls is small and, as in H. bicolor, probably not sufficient to support ecological resource partitioning. As in H. bicolor, social character displacement and selection for non-interference may have played a large role in the diversification of the P. parnellii complex, particularly in northern South America where there are no obvious barriers to prevent contact between all three groups. If the Central American group has diverged in allopatry, it is likely that the variation in the constant frequency component has arisen by drift. It is not known what threshold of variation is used for social recognition in P. parnellii, but estimates from Old World bats suggest that the constraints of high-duty cycle echolocation limit the possible variation more than in low-duty cycle species . Future research on inter- and intraspecific call divergence and the social aspects of call recognition and discrimination will prove interesting and may support an allopatric or sympatric origin of these groups.
Echolocation: a mode of reinforcement and speciation?
Theoretically, for speciation to occur despite substantial gene flow, reinforcement and a strong mate choice character are almost certainly required. In bats, non-morphological traits may be very important in speciation. In some potential cryptic species complexes, for instance Saccopteryx bilineata (family Emballonuridae) , olfactory cues may be particularly important. In bats that rely on echolocation, divergence between echolocation calls may reduce signal competition and influence prey selection [29, 70] but also change mate recognition , creating an almost instantaneous form of pre-zygotic isolation. For reinforcement to directly influence speciation, a genetic association (linkage) between the ecological trait under selection and the mating signal is required [70, 72], a situation which should be disrupted by recombination . This problem is averted if the diverging trait is controlled by the same genetic loci which become fixed in the diverging populations (one allele model of Felsenstein ). This is particularly effective when the characters governing both pre- and post-zygotic isolation are the same, so-called “magic traits” [74, 75], and direct selection has pleiotropic effects . Echolocation may meet both of these criteria  and, in this context, changes in echolocation could rapidly lead to speciation.
Ecological character displacement (selection for increased ecological niche separation which indirectly influences mate choice ) may also speed the process of differentiation. The divergence of echolocation call design may start as selection against signal interference between populations [29, 33] but may be taken over by selection for mate recognition. In this scenario, speciation can be swift in the presence of gene flow whether selection is initially acting on niche specialization or mate recognition [70, 76].
In our analysis, Groups 3 and 4 currently occupy sympatric distributions (Figure 3). Elmer and Meyer  outline four “gold standard” criteria for the hypothesis of sympatric speciation: 1) sympatric distributions, 2) a reciprocally monophyletic relationship between the taxa (sister species) with respect to others in the complex, 3) reproductive isolation and, 4) a setting where allopatric divergence is unlikely. Groups 3 and 4 appear to meet at least the first three criteria, but several lines of evidence could refute sympatric speciation. First, the relationship between individuals from the Brazilian Amazon and French Guiana is unknown. If a group from these areas is sister to Group 3 or Group 4 (raising the possibility of historical allopatric ranges), the hypothesis of sympatric speciation may be refuted. Additionally, pre-zygotic isolation should develop before post-zygotic isolation in cases of sympatric speciation . The presence of only one hybrid suggests that hybrids are rare but does not provide evidence for the cause. Searching for evidence of F2 individuals could illuminate this .
The present study has established that P. parnellii from the mainland regions of Central and northern South America represents four distinct species with distinct genetic characters and divergent morphology and echolocation. With the exception of a single potential hybrid, they appear to be reproductively isolated and meet the biological species concept  and certainly meet the genetic species concept as outlined for mammals . We suggest the elevation of P. mesoamericanus as an appropriate name for individuals in Central America, however the taxonomy in South America remains highly complex and none of these species correspond with P. parnellii. High-duty cycle echolocation has always been considered an Old World trait among bats with only this single example in the New World. The presence of at least five high-duty cycle species with a single origin in the New World presents an interesting case of adaptive radiation in only a few million years. This complex is also an excellent, convergently-evolved model for comparison with species undergoing rapid radiation via high-duty cycle echolocation in the Old World. The potential of this system for the study of allopatric and sympatric divergence makes it a very exciting complex for both evolutionary and ecological research.
This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) through Discovery Grants to MBF and PDNH and a Canadian Graduate Scholarship and Post-Doctoral Fellowship to ELC. Additional funding for this research was provided by Bat Conservation International, Genome Canada through the Ontario Genomics Institute and the Biodiversity Institute of Ontario. This research was made possible by generous access to the collections of the ROM and research support from Dr. Burton Lim and Dr. Mark Engstrom. Field research was conducted in Lamanai Belize, Windsor House Jamaica facilitated by Dr. Susan Koenig and Mike Schwartz, and in the Area de Conservación de Guanacaste in Costa Rica facilitated by Dr. Dan Janzen, Winnie Halwachs and Roger Blanco Segura and the staff at Santa Rosa Sector. We appreciate the donation of echolocation data from Drs. Burton Lim and Holger Goerlitz and forearm data from Dr. Susan Koenig. We also wish to thank numerous anonymous reviewers for their comments and Dr Robin Floyd for additional editorial suggestions.
- Stehli FG, Webb SD: A kaleidoscope of plates, faunal and floral dispersals, and sea level changes. The Great American Biotic Interchange. Edited by: Stehli FG, Webb SD. 1985, New York: Plenum Press, 3-15.View ArticleGoogle Scholar
- Gregory-Wodzicki KM: Uplift history of the Central and Northern Andes: A review. Geol Soc Am Bull. 2000, 112: 1091-1105.View ArticleGoogle Scholar
- Sutherland GD, Harestad AS, Price K, Lertzman KP: Scaling of Natal Dispersal Distances in Terrestrial Birds and Mammals. Conserv Ecol. 2000, 4: 16-Google Scholar
- Munguía M, Peterson AT, Sánches-Cordero V: Dispersal limitation and geographical distributions of mammal species. J Biogeogr. 2008, 35: 1879-1887.View ArticleGoogle Scholar
- Cruzan MB, Templeton AR: Paleoecology and coalescence: phylogeographic analysis of hypotheses from the fossil record. Trends Ecol Evol. 2000, 15: 491-496.PubMedView ArticleGoogle Scholar
- Hoffmann FG, Owen JG, Baker RJ: mtDNA perspective of chromosomal diversification and hybridization in Peters’ tent-making bat (Uroderma bilobatum: Phyllostomidae). Mol Ecol. 2003, 12: 2981-2993.PubMedView ArticleGoogle Scholar
- Baker RJ, Bradley RD: Speciation in mammals and the Genetic Species Concept. J Mammal. 2006, 87: 643-662.PubMed CentralPubMedView ArticleGoogle Scholar
- Clare EL, Lim BK, Fenton MB, Hebert PDN: Neotropical bats: Estimating Species Diversity with DNA Barcodes. PLoS One. 2011, 6: e22648-PubMed CentralPubMedView ArticleGoogle Scholar
- Clare EL: Cryptic species? Patterns of Maternal and Paternal Gene Flow in Eight Neotropical Bats. PLoS One. 2011, 6: e21460-PubMed CentralPubMedView ArticleGoogle Scholar
- Avise JC, Walker D: Species realities and numbers in sexual vertebrates: Perspectives from an asexually transmitted genome. P Natl Acad Sci-Biol. 1999, 96: 992-995.View ArticleGoogle Scholar
- Witt JDS, Threloff DL, Hebert PDN: DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus: implications for desert spring conservation. Mol Ecol. 2006, 15: 3073-3082.PubMedView ArticleGoogle Scholar
- Floyd R, Abebe E, Papert A, Blaxter M: Molecular barcodes for soil nematode identification. Mol Ecol. 2002, 11: 839-850.PubMedView ArticleGoogle Scholar
- Reeder DM, Helgen KM, Wilson DE: Global trends and biases in new mammal species discoveries. Occasional Papers The Museum of Texas Tech University. 2007, 269: 1-35.Google Scholar
- Randi E, D’Huart J-P, Lucchini V, Aman R: Evidence of two genetically deeply divergent species of warthog, Phacochoerus africanus and P. aethiopicus (Artiodactyla: Suiformes) in East Africa. Mamm Biol. 2002, 67: 91-96.Google Scholar
- Xu X, Amason U: The mitochondrial DNA molecule of Sumatran orangutan and a molecular proposal for two (Bornean and Sumatran) species of orangutan. J Mol Evol. 1996, 43: 431-437.PubMedView ArticleGoogle Scholar
- Brown DM, Brenneman RA, Koepfli K-P, Pollinger JP, Milá B, Georgiadis NJ, Louis EE, Grether GF, Jacobs DK, Wayne RK: Extensive population genetic structure in the giraffe. BMC Biol. 2007, 5: 57-PubMed CentralPubMedView ArticleGoogle Scholar
- Amato G, Egan MG, Rabinowitz A: A new species of muntjac, Muntiacus putaoensis (Artiodactyla: Cervidae) from northern Myanmar. Anim Conserv. 1999, 2: 1-7.View ArticleGoogle Scholar
- Wada S, Oishi M, Yamada TK: A newly discovered species of living baleen whale. Nature. 2003, 426: 278-281.PubMedView ArticleGoogle Scholar
- Dalebout ML, Mead JG, Baker CS, Baker AN, Helden AL: A new species of beaked whale Mesoplodon perrini sp.N. (Cetacea: Ziphiidae) discovered through phylogenetic analyses of mitochondrial DNA sequences. Mar Mammal Sci. 2002, 18: 577-608.View ArticleGoogle Scholar
- Roca AL, Georgiadis N, Pecon-Slattery J, O’Brien SJ: Genetic evidence for two species of elephant in Africa. Science. 2001, 293: 1473-1477.PubMedView ArticleGoogle Scholar
- Jones G: Acoustic signals and speciation: the roles of natural and sexual selection in the evolution of cryptic species. Adv Stud Behav. 1997, 26: 317-354.View ArticleGoogle Scholar
- Barratt EM, Deaville R, Burland TM, Bruford MW, Jones G, Racey PA, Wayne RK: DNA answers the call of pipistrelle bat species. Nature. 1997, 387: 138-139.PubMedView ArticleGoogle Scholar
- Mayer F, von Helversen O: Cryptic diversity in European bats. P Roy Soc Lond B Bio. 2001, 268: 1825-1832.View ArticleGoogle Scholar
- Francis CM, Borisenko AV, Ivanova NV, Eger JL, Lim BK, Guillén-Servent A, Kruskop SV, Mackie I, Hebert PDN: The role of DNA barcodes in understanding and conservation of mammal diversity in Southeast Asia. PLoS One. 2010, 5: e12575-PubMed CentralPubMedView ArticleGoogle Scholar
- Borisenko AV, Lim BK, Ivanova NV, Hanner RH, Hebert PDN: DNA barcoding in surveys of small mammal communities: a field study in Suriname. Mol Ecol Resour. 2008, 8: 471-479.PubMedView ArticleGoogle Scholar
- Clare EL, Lim BK, Engstrom MD, Eger JL, Hebert PDN: DNA barcoding of Neotropical bats: species identification and discovery within Guyana. Mol Ecol Notes. 2007, 7: 184-190.View ArticleGoogle Scholar
- Long GR, Schnitzler H-U: Behavioural audiograms from the bat, Rhinolophus ferrumequinum. J Comp Physiol A. 1975, 100: 211-219.View ArticleGoogle Scholar
- Schuller G, Pollak GD: Disproportionate frequency representation in the inferior colliculus of doppler-compensating greater horseshoe bats: evidence for an acoustic fovea. J Comp Physiol A. 1979, 132: 47-54.View ArticleGoogle Scholar
- Kingston T, Rossiter SJ: Harmonic-hopping in Wallacea's bats. Nature. 2004, 429: 654-657.PubMedView ArticleGoogle Scholar
- Kober R, Schnitzler H-U: Information in sonar echoes of fluttering insects available for echolocation bats. J Acoust Soc Am. 1990, 87: 882-896.View ArticleGoogle Scholar
- Herd RM: Pteronotus parnellii. Mammalian Species. 1983, 209: 1-5.View ArticleGoogle Scholar
- Puechmaille SJ, Gouilh MA, Piyapan P, Yokubol M, Mie KM, Bates PJ, Satasook C, Nwe T, Bu SSH, Mackie IJ, Petit EJ, Teeling EC: The evolution of sensory divergences in the context of limited gene flow in the bumblebee bat. Nature Communications. 2011, 2: 573-PubMed CentralPubMedView ArticleGoogle Scholar
- Kingston T, Lara MC, Jones G, Akbar Z, Kunz TH, Schneider CJ: Acoustic divergence in two cryptic Hipposideros species: a role for social selection?. P Roy Soc Lond B Bio. 2001, 268: 1381-1386.View ArticleGoogle Scholar
- Houston RD, Boonman AM, Jones G: Do echolocation signal parameters restrict bats’ choice of prey?. Echolocation in bats and dolphins. Edited by: Thomas JA, Moss CF, Vater M. 2004, Chicago: The University of Chicago Press, 339-345.Google Scholar
- Jones G, Barlow KE: Cryptic species of echolocating bats. Echolocation in bats and dolphins. Edited by: Thomas JA, Moss CF, Vater M. 2004, Chicago: The University of Chicago Press, 345-349.Google Scholar
- Bradley RD, Baker RJ: A test of the genetic species concept: cytochrome b sequences and mammals. J Mammal. 2001, 82: 960-973.View ArticleGoogle Scholar
- Solari S, Baker RJ: Mitochondrial DNA sequence, karyotypic and morphological variation in the Carollia castanea species complex (Chiroptera: Phyllostomidae) with description of a new species. Occasional Papers The Museum of Texas Tech University. 2006, 254: 1-16.Google Scholar
- Lewis-Oritt N, Porter CA, Baker RJ: Molecular systematics of the family Mormoopidae (Chiroptera) based on cytochrome b and recombination activating gene 2 sequences. Mol Phylogenet Evol. 2001, 20: 426-436.PubMedView ArticleGoogle Scholar
- Van Den Bussche RA, Hoofer SR, Simmons NB: Phylogenetic relationships of mormoopid bats using mitochondrial gene sequences and morphology. J Mammal. 2002, 83: 40-48.View ArticleGoogle Scholar
- Lim BK, Engstrom MD, Bickham JW, Patton JC: Molecular phylogeny of New World sheath-tailed bats (Emballonuridae: Diclidurini) based on loci from the four genetic transmission systems of mammals. Biol J Linn Soc. 2008, 93: 189-209.View ArticleGoogle Scholar
- Baker RJ, Porter CA, Patton JC, Van Den Bussche RA: Systematics of bats of the family Phyllostomidae based on RAG2 DNA sequences. Occasional Papers Museum of Texas Tech University. 2000, 202: 1-16.Google Scholar
- Clement M, Posada D, Crandall KA: TCS: a computer program to estimate gene genealogies. Mol Ecol. 2000, 9: 1657-1659.PubMedView ArticleGoogle Scholar
- Posada D, Crandall KA: MODELTEST: testing the model of DNA substitution. Bioinformatics. 1998, 14: 817-818.PubMedView ArticleGoogle Scholar
- Sánchez R, Serra F, Tárraga J, Medina I, Carbonell J, Pulido L, de María A, Capella-Gutíerrez S, Huerta-Cepas J, Gabaldón T, Dopazo J, Dopazo H: Phylemon 2.0: a suite of web-tools for molecular evolution, phylogenetics, phylogenomics and hypotheses testing. Nucl Acids Res. 2011, 39: 1-5. 10.1093|nar|gkr408.View ArticleGoogle Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704.PubMedView ArticleGoogle Scholar
- Shimodaira H, Hasegawa M: Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Mol Biol Evol. 1999, 16: 1114-1116.View ArticleGoogle Scholar
- Huelsenbeck JP, Ronquist F: MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics. 2001, 17: 754-755.PubMedView ArticleGoogle Scholar
- Drummond AJ, Rambaut A: BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol. 2007, 7: 214-PubMed CentralPubMedView ArticleGoogle Scholar
- Smith MF, Patton JL: Phylogenetic relationships and the radiation of Sigmodontinae rodents in South America: evidence from cytochrome b. J Mamm Evol. 1999, 6: 89-128.View ArticleGoogle Scholar
- Hoffmann FG, Baker RJ: Comparative phylogeography of short-tailed bats (Carollia: Phyllostomidae). Mol Ecol. 2003, 12: 3403-3414.PubMedView ArticleGoogle Scholar
- Hulva P, Horáček P, Strelkov PP, Benda P: Molecular architecture of Pipistrellus pipistrellus / Pipistrellus pygmaeus complex (Chiroptera: Vespertilionidae): further cryptic species and Mediterranean origin of the divergence. Mol Phylogenet Evol. 2004, 32: 1023-1035.PubMedView ArticleGoogle Scholar
- Martins FM, Templeton AJ, Pavan ACO, Kohlbach BC, Morgante JS: Phylogeography of the common vampire bat (Desmodus rotundus): marked population structure Neotropical Pleistocene vicariance and incongruence between nuclear and mtDNA markers. BMC Evol Biol. 2009, 9: 294-PubMed CentralPubMedView ArticleGoogle Scholar
- Smith JD: Systematics of the chiropteran family Mormoopidae. Miscellaneous Publications of the University of Kansas Museum of Natural History. 1972, 56: 1-132.Google Scholar
- Skowronski MD, Fenton MB: Model-based automated detection of echolocation calls using the link detector. J Acoust Soc Am. 2008, 124: 328-336.PubMedView ArticleGoogle Scholar
- R Development Core Team: R: A language and environment for statistical computing. 2008, Vienna, Austria: R Foundation for Statistical Computing, ISBN 3-900051-07-0, URL http://www.R-project.orgGoogle Scholar
- PASW Ontario Universities Student SPSS Version 18.0 SPSS Inc. 2009, Chicago: an IBM CompanyGoogle Scholar
- Hasegawa M, Kishino H, Yano T: Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evo. 1985, 22: 160-174.View ArticleGoogle Scholar
- Tavaré S: Some Probabilistic and Statistical Problems in the Analysis of DNA Sequences. Lectures on Mathematics in the Life Sciences. 1986, American Mathematical Society, 57-86.Google Scholar
- Ditchfield AD: The comparative phylogeography of Neotropical mammals: patterns of intraspecific mitochondrial DNA variation among bats contrasted to nonvolant small mammals. Mol Ecol. 2000, 9: 1307-1318.PubMedView ArticleGoogle Scholar
- Rivers NM, Butlin RK, Altringham JD: Genetic population structure of Natterer’s bats explained by mating at swarming sites and philopatry. Mol Ecol. 2005, 14: 4299-4312.PubMedView ArticleGoogle Scholar
- Berthier P, Excoffier L, Ruedi M: Recurrent replacement of mtDNA and cryptic hybridization between two sibling bat species Myotis myotis and Myotis blythii. Proc. R. Soc. B. 2006, 273: 3101-3109.PubMed CentralPubMedView ArticleGoogle Scholar
- Krausz C, McElreavey K: Y Chromosome and male infertility. Front Biosci. 1999, 4: e1-e8.PubMedView ArticleGoogle Scholar
- Simmons NB: Order Chiroptera. Mammal Species of the World: a Taxonomic and Geographic Reference. Edited by: Wilson DE, Reeder DM. 2005, Baltimore Maryland: Johns Hopkins University Press, 312-529. 3Google Scholar
- Gutiérrez EE, Molinari J: Morphometrics and taxonomy of bats of the genus Pteronotus (subgenus Phyllodia) in Venezuela. J Mamm. 2008, 89: 292-305.View ArticleGoogle Scholar
- Meiklejohn CD, Montooth KL, Rand DM: Positive and negative selection on the mitochondrial genome. Trends Genet. 2007, 23: 259-263.PubMedView ArticleGoogle Scholar
- Rogers JJW: A history of continents in the past three billion years. J Geol. 1996, 104: 91-107.View ArticleGoogle Scholar
- Vdovin O, Rial JA, Levshin AL, Ritzwoller MH: Group-velocity tomography of South America and the surrounding oceans. Geophys J Int. 1999, 136: 324-340.View ArticleGoogle Scholar
- Huber O, Foster MN: Conservation priorities for the Guayana shield: 2002 Consensus. 2002, Washington DC: Conservation International. Center for Applied Biodiversity ScienceGoogle Scholar
- Dávalos LM: The geography of diversification in the mormoopids (Chiroptera: Mormoopidae). Biol J Linn Soc. 2006, 88: 101-118.View ArticleGoogle Scholar
- Hoskin CJ, Higgie M: Speciation via species interactions: the divergence of mating traits within species. Ecol Lett. 2010, 13: 409-420.PubMedView ArticleGoogle Scholar
- Siemers MB, Beedholm K, Diets C, Dietz I, Ivanova T: Is species identity, sex, age or individual quality conveyed by echolocation call frequency in European horseshoe bats?. Acta Chiropterol. 2005, 7: 259-274.View ArticleGoogle Scholar
- Rundle HD, Nosil P: Ecological Speciation. Ecol Lett. 2005, 8: 336-352.View ArticleGoogle Scholar
- Felsenstein J: Skepticism towards Santa Rosalia, or why are there so few kinds of animals?. Evolution. 1981, 35: 124-138.View ArticleGoogle Scholar
- Gavrilets S: Fitness landscapes and the origin of species. 2004, New Jersey: Princeton University Press. PrincetonGoogle Scholar
- Servedio MR, Van Doorn GS, Kopp M, Frame AM, Nosil P: Magic traits in speciation: “magic” but not rare?. Trends Ecol Evol. 2011, 26: 389-397.PubMedView ArticleGoogle Scholar
- Ortiz-Barrientos D, Grealy A, Nosil P: The genetics and ecology of reinforcement: implications for the evolution of prezygotic isolation in sympatry and beyond. Ann NY Acad Sci. 2009, 1168: 156-182.PubMedView ArticleGoogle Scholar
- Elmer KR, Meyer A: Sympatric speciation without borders?. Mol Ecol. 2010, 19: 1991-1993.PubMedView ArticleGoogle Scholar
- Crow KD, Munehara H, Bernard G: Sympatric speciation in a genus of marine reef fishes. Mol Ecol. 2010, 19: 2089-2105.PubMedView ArticleGoogle Scholar
- Mayr E: Systematics and the origin of species. 1942, New York: Columbia University PressGoogle Scholar
- Ivanova NV, deWaard JR, Hebert PDN: An inexpensive, automation-friendly protocol for recovering high-quality DNA. Mol Ecol Notes. 2006, 6: 998-1002.View ArticleGoogle Scholar
- Ivanova NV, Zemlak TS, Hanner RH, Hebert PDN: Universal primer cocktails for fish DNA barcoding. Mol Ecol Notes. 2007, 7: 544-548.View ArticleGoogle Scholar
- Pfunder M, Holzgang O, Frey JE: Development of microarray-based diagnostics of voles and shrews for use in biodiversity monitoring studies, and evaluation of mitochondrial cytochrome oxidase 1 vs. cytochrome b as genetic markers. Mol Ecol. 2004, 13: 1277-1286.PubMedView ArticleGoogle Scholar
- Messing J: New M13 vectors for cloning. Method Enzymol. 1983, 101: 20-78.View ArticleGoogle Scholar
- Hellborg L, Ellegren H: Y chromosome conserved anchored tagged sequences (YCATS) for the analysis of mammalian male-specific DNA. Mol Ecol. 2003, 12: 283-291.PubMedView ArticleGoogle Scholar
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