Genotype and diet shape resistance and tolerance across distinct phases of bacterial infection
© Howick and Lazzaro; licensee BioMed Central Ltd. 2014
Received: 7 February 2014
Accepted: 18 March 2014
Published: 22 March 2014
Host defense against pathogenic infection is composed of resistance and tolerance. Resistance is the ability of the host to limit a pathogen burden, whereas tolerance is the ability to limit the deleterious effects of a given pathogen burden. This distinction recognizes that the fittest host does not necessarily have the most aggressive immune system, suggesting that host-pathogen co-evolution involves more than an escalating arms race between pathogen virulence factors and host antimicrobial activity. How a host balances resistance and tolerance and how this balance influences the evolution of host defense remains unanswered. In order to determine how genotype-by-diet interactions and evolutionary costs of each strategy may constrain the evolution of host defense, we measured survival, fecundity, and pathogen burden over five days in ten genotypes of Drosophila melanogaster reared on two diets and infected with the Gram-negative bacterial pathogen Providencia rettgeri.
We demonstrated two distinct phases of infection: an acute phase that consists of high mortality, low fecundity, and high pathogen loads, and a chronic phase where there was a substantial but stable pathogen load and mortality and fecundity returned to uninfected levels. We demonstrated genetic variation for resistance in both phases of infection, but found genetic variation for tolerance only in the acute phase. We found genotype-by-diet interactions for tolerance, especially in the acute phase, but genotype-by-diet interaction did not significantly shape resistance. We found a diet-dependent positive relationship between resistance and tolerance and a weak evolutionary cost of resistance, but did not detect any costs of tolerance.
Existing models of tolerance and resistance are overly simplistic. Multi-phase infections such as that studied here are rarely considered, but we show important differences in determination and evolutionary constraints on tolerance and resistance over the two phases of infection. Our observation of genetic variation for tolerance is inconsistent with simple models that predict evolutionary fixation of tolerance alleles, and instead indicate that genetic variation for resistance and tolerance is likely to be maintained by non-independence between resistance and tolerance, condition-dependent evolutionary costs, and environmental heterogeneity.
KeywordsTolerance Resistance Host-pathogen interactions Drosophila Genotype-by-environment interactions
A host can deal with an infection by directly limiting a pathogen burden (resistance) or by withstanding the negative consequences of the given burden (tolerance). Biomedically, tolerance implies that the host with the most aggressive immune system may not necessarily be the healthiest . Evolutionarily, tolerance expands the range of strategies a host could employ to maximize fitness under infection conditions, and may impose evolutionary constraints, requiring the host to balance these potentially antagonistic strategies. From the pathogen’s perspective, tolerance may relax or remove selection imposed by the canonical immune system, potentially resulting in a neutral or positive effect on pathogen fitness [2–4].There is widespread evidence that tolerance is an important feature of host defense, but how this strategy evolves in concert with resistance and other life-history traits in animals, especially across variable environments, remains unknown.
From the host perspective, the superficial outcome of both resistance and tolerance can be the same: the retention of fitness. However, the long-term ecological and evolutionary dynamics are predicted to have very different outcomes. Importantly, resistance is expected to have a direct negative effect on pathogen fitness, whereas tolerance will have a neutral or positive effect [2–4]. This increase in pathogen fitness promoted by host tolerance could result in increased pathogen prevalence, which may increase the benefit of alleles that confer tolerance in the host, possibly offsetting any costs of those alleles. Oppositely, hosts with costly resistance alleles may reduce pathogen prevalence to levels where the resistant allele is no longer beneficial [2–5]. Theoretical work has shown that this negative feedback loop under a resistance strategy could result in the maintenance of polymorphism for resistance alleles, whereas the positive feedback loop created when a tolerance strategy is employed is more likely to result in the fixation of tolerance alleles in the population . In this study, we measured genetic variation for both tolerance and resistance to empirically test this simple model. Our prediction was that we would find little genetic variation for tolerance if the simple positive feedback model is true, but that negative feedback loops would result in considerable variation for resistance.
While the predicted evolutionary outcomes of tolerance and resistance are very different, the natural dynamics are complicated by both internal constraints and external variability. The model proposed by Roy  assumes that resistance and tolerance alleles are independent, that there is only a single locus determining each strategy, and that each strategy exhibits the same evolutionary cost. The model also assumes a single, non-evolving, pathogen genotype and a constant environment. Theoretical work has relaxed some these simplifications [6–8], showing that variation in costs of the defense strategies could maintain variation in both tolerance and resistance, but these issues have not been empirically addressed in animals. We empirically address these complexities by quantifying costs, response to altered dietary environment, genotype-by-diet interactions, and the relationship between these two strategies.
Disentangling defense into separate components of resistance and tolerance, and understanding how these distinct strategies are related to each other, is crucial to understanding the evolution of host defense. However, first we must know how tolerance manifests and how it should be quantified. Empirical studies in animals have mainly focused on measures of health as estimates of tolerance, including survival , weight , and red blood cell count , whereas plant work has focused on more direct fitness estimates such as total fruit set  and seed production . These different metrics of tolerance could have different evolutionary outcomes because they may differentially affect pathogen fitness  and may trade off with different aspects of host physiology. Statistically, tolerance has been defined as the slope of the line where the health or fitness estimate is plotted against pathogen burden. Three recent studies have explicitly tested for natural genetic variation for tolerance in animals using this framework. Two found evidence for variation in tolerance [11, 15], whereas one did not .
Although tolerance in practical terms has been studied in agriculture for over 100 years [16, 17], recent interest in evolutionary questions about tolerance in natural systems was stimulated by a study in morning glory that demonstrated a negative relationship between resistance and tolerance . However, this negative relationship does not seem to be generalizable, and the current consensus in the plant literature leans towards a positive relationship between these two strategies, where a host allocates defense resources to both resistance and tolerance [19, 20]. In animals, Räberg et al.  found a negative relationship between resistance (peak pathogen load) and tolerance (weight and red blood cells) in laboratory mice infected with Plasmodium chabaudi. Tolerance and resistance may be correlated because of pleiotropic mechanisms, linkage disequilibrium, or physiological constraints. Inconsistencies in the relationship between resistance and tolerance could result from system-specific mechanisms  and/or different definitions or metrics of tolerance and resistance.
Dietary environment and metabolic status can affect defense through both tolerance and resistance. Resistance is affected by dietary environment [21, 22], and there is antagonism between defense and growth/tissue repair [23–26] as well as reproduction . We hypothesized that a tradeoff between resistance and tolerance might be mediated by processes that are responsive to dietary environment, with the underlying assumption that tolerance strategies include tissue repair and growth, while resistance includes canonical immunity. Alternatively, a positive relationship between these two strategies would suggest that increased resource allocation to defense results in simultaneously improved resistance and tolerance, as would be expected if the underlying mechanisms of these strategies were overlapping. We predicted that dietary environment would alter both tolerance and resistance, but that the relationship between the two might change in a diet-dependent manner.
In the present study, we were able to identify genetic variation for both tolerance and resistance and show that maintenance of this polymorphism could result from a balanced optimum between these two strategies, shaped by evolutionary costs of defense and genotype-by-environment interactions. We hypothesized that dietary environment would alter the balance between tolerance and resistance, and that different environments may favor different defense strategies. We estimated tolerance both as functions of survival and fecundity under infection conditions to understand whether these are in fact different processes and distinct forms of tolerance. We followed these traits over time, developing a framework for defense that acknowledges that infection is not a binary state but a dynamic process consisting of multiple phases of infection. This study sheds light on the evolvability of tolerance and resistance, and how these distinct strategies may influence the evolution of host-pathogen interactions.
Dynamics of P. rettgeriinfection
Possible terms included in regression analyses
Fixed or random
F1 cross genotype of flies
Diet that flies were reared and phenotyped on
Daily bacterial load, natural log transformed
Daily fecundity of uninfected flies
Differential effect of diet on each genotype
Differential effect of pathogen load on each genotype
Differential effect of pathogen load on each diet
Differential dynamics on each diet
Differential dynamics of tolerance
Differential effect of diet on tolerance
Differential effect of diet on tolerance dynamics
Model F tested the effect of diet on resistance
Model D tested the effect of diet on mortality tolerance
Model B tested the effect of diet on fecundity tolerance
Is there genetic variation for tolerance and resistance?
After initial characterization of the dynamics of bacterial load, survival, and reproduction, we sought to determine whether the host D. melanogaster population was genetically variable for defense quality, and whether that defense quality varied with diet. The 10 F1 outbred genotypes in our study are derived from the Drosophila genetic reference panel (DGRP), a panel of 192 inbred lines that were each created from an independent single female collected in Raleigh, NC, USA . To avoid effects of inbreeding, we chose 10 of these lines at random and established outbred F1 genotypes using a ring cross design, such that each of the 10 chosen DGRP lines contributed a mother to one experimental genotype and a father to another (Figure 1). All phenotypes were genetically polymorphic among our outbred progeny (Figure 2). Three linear models were fitted to the data to determine the magnitudes of genetic variation for resistance (systemic pathogen load), fecundity tolerance (the host’s ability to produce offspring given the pathogen burden), and mortality tolerance (the host’s ability to survive a given pathogen burden). Daily fecundity tolerance was estimated as the number of offspring produced each day normalized by the bacterial load estimated for the genotype on that day. Mortality tolerance was measured as the daily mortality rate per fly normalized to the bacterial load. The terms included in these models and their interpretations are given in Table 1. Separate models were run on the data from the acute and chronic phases of infection.
Model E tested for genetic variation in resistance
Model A tested for genetic variation in fecundity tolerance
Model C tested for genetic variation in mortality tolerance
Complex relationship between defense strategies and diet
We hypothesized that the nutritional quality of the rearing diet might influence the quality of defense, either through resistance or tolerance. To test this, we evaluated defense quality after rearing on either high-sugar or low-sugar diets. Both Diet and Day post-infection were significant predictors of Load (Model F, Table 2; Diet: p = 0.0012, Day: p < 0.001), but there was no significant interaction between Diet and Day. This suggests that the dynamics of the resistance phenotype (i.e., when the pathogen peaks, and how it is reduced in the host entering into the chronic phase) does not change across the diets used in this experiment (p = 0.330). Diet also significantly influenced mortality tolerance (Model D, Table 3; Diet*Load, p = 0.007), with higher mortality and thus lower tolerance observed on the high-sugar diet. In contrast, we found no evidence for a dietary effect on fecundity tolerance (Model B, Table 4; Bacterial load*Diet: p = 0.709). Thus, despite the fact that both fecundity and bacterial load are independently sensitive to diet, the relationship between them is not.
Given that diet shapes resistance and mortality tolerance, we hypothesized that the quantitative effects of diet depend on host genotype. We found evidence for genotype-by-diet determination of fecundity and mortality tolerance (Fecundity Tolerance: Model A, Table 6, Genotype*Diet*Load: p = 0.002; Mortality Tolerance, Model C, Table 7, Genotype*Diet*Load: p < 0.001) in the acute phase of infection. We also found a marginal genotype-by-diet effect on fecundity tolerance during the chronic phase of infection (Model A, Table 6; Genotype*Diet*Load: p = 0.044). This apparent interaction effect on fecundity tolerance disappears from the acute phase of the infection, if we reanalyze the data with per-fly daily fecundity estimated from the number of females that are alive at the end of each day instead of the number of flies that are alive at the beginning of the day (Additional file 1; Genotype*Diet*Load: acute: p = 0.390, chronic: p = 0.025). This indicates that the effect of the genotype-by-diet interaction on fecundity tolerance in the acute infection may be driven by the effect of the interaction on daily mortality. We did not find evidence for a genotype-by-diet interaction influencing resistance in either the acute or chronic phase of infection (Model E, Table 5; acute: p = 0.378, chronic: p = 0.305). Other experiments studying resistance against P. rettgeri with more genotypes and more extreme diets have found genotype-by-diet effects for resistance (unpublished results). This suggests our study may have lacked power to detect these effects, or that these effects are only detectable when the diets span a greater range of nutritional quality.
Relationship between resistance and tolerance
We found two distinct phases of infection over the five days that defense was assessed. The acute phase (Days 1–3) was characterized by a high pathogen burden, severe mortality, and a reduction in fecundity. The chronic phase (Days 4–5) consisted of a still substantial but stable pathogen burden that was in no way predictive of mortality or fecundity. We found genetic variation for resistance, mortality tolerance, and fecundity tolerance in the acute phase of the infection, but only for resistance in the chronic phase. Experimental diet significantly influenced resistance and mortality tolerance during the acute phase of the infection. However, fecundity tolerance was not sensitive to diet. That is, even though diet altered both pathogen load and fecundity, it did so proportionally such that the relationship between load and fecundity (and hence fecundity tolerance) was unchanged. Using overall estimates of resistance and tolerance in the acute phase of infection, we found a positive relationship between resistance and tolerance, although the strength of this relationship was diet-dependent. We also demonstrate a weak evolutionary cost of resistance, but fail to detect any cost of tolerance.
During the chronic phase of infection, pathogen levels remained constant. This finding suggests either that the host and pathogen have reached an equilibrium where the immune system is killing bacteria at the same rate that the bacteria are replicating or that the bacteria have stopped replicating and are no longer exposed or sensitive to host immunity. We speculate that the latter may be occurring, because mortality and fecundity both return to near uninfected levels during the chronic phase. Further understanding the patterns of infection from the perspective of both host tolerance and pathogen virulence will clarify how both players interact to create host tolerance.
Current theoretical models describing variation of resistance and tolerance in the context of host-pathogen coevolution assume a single state of infection [2–4, 6, 7, 14]. The empirical distinction between the acute and chronic phases of infection demonstrated here emphasizes the importance of looking at disease dynamics over time before extrapolating predictions about the spread of disease and evolution of defense in the wild. If transmission of P. rettgeri were to occur during the chronic phase of infection, then our results could be interpreted as supporting a model where tolerance alleles have fixed in the population. Infection tolerance through static chronic infection, which may not have major fitness consequences, should be integrated in future theoretical work on defense and infectious disease dynamics.
Consistent with previous studies (e.g., [29–31], we found genetic variation for resistance. We also found genetic variation for fecundity tolerance and for mortality tolerance. Previous studies in animals have found genetic variation in tolerance but see [10, 11, 15] and genetic variation for fecundity tolerance has been repeatedly observed in both the plant and animal literature [15, 32, 33], observations which we recapitulate in the acute (but not chronic) phase of infection. The continued presence of genetic variation for tolerance is inconsistent with basic theoretical models predicting tolerance alleles to be more likely to fix in the population than resistance alleles [3, 4]. Non-independence between resistance and tolerance, or genotype-by-environment interactions may explain this inconsistency.
Although diet altered all three phenotypes measured (bacterial load, survival, and fecundity), it did not alter fecundity tolerance. In other words, the relationship between fecundity and pathogen load did not change across the two diets used in this experiment. However, mortality tolerance was very sensitive to diet. We expected that both fecundity and mortality tolerance would be sensitive to diet because both egg production and survival are regulated by metabolic pathways such as insulin-like signaling, which are responsive to alteration in dietary sugar (e.g. ). We observed that host females do not completely stop egg production under infection conditions, nor do we see any evidence for “egg dumping” – a rapid increase in egg output to maximize short-term fitness upon infection . Instead, we observe only a minimal reduction in fecundity after infection, implying that females continue to invest in short-term reproductive output even though it may increase the risk of dying from infection and hence potentially limit lifetime fecundity.
We observed genotype-by-diet effects on mortality tolerance and fecundity tolerance, but saw no significant genotype-by-diet interaction for resistance. While increasing dietary glucose increased pathogen burden, there was little evidence for genetic variation in immunological sensitivity to dietary sugar in this study. Since there are significant genotype-by-diet effects on tolerance but not resistance during acute infection, we infer that the genes underlying tolerance are likely to function outside of the canonical resistance pathways.
In general, we found a positive relationship between resistance and tolerance, which supports the hypothesis that resource allocation to defense contributes to both resistance and tolerance . Although, conceptually, a tradeoff between resistance and tolerance is easy to imagine and has some theoretical support (, but see [7, 8, 36, 37]), the empirical evidence is very mixed. Several high-impact papers have demonstrated a negative relationship between the two strategies [11, 12, 18]. However, a meta-analysis of 31 ecological and agricultural studies of herbivore defense strategies did not find a significant relationship between resistance and tolerance , and several studies have demonstrated a positive relationship between the two strategies [39, 40]. This lack of consensus may be a result of idiosyncrasies of specific host and pathogen combinations , as well as to employment of different measures of tolerance and resistance across studies. Our observation that the strength of the relationship between resistance and tolerance is diet-dependent suggests that environmental heterogeneity could decouple these two traits and allow them to evolve independently.
The observed tradeoff between pre-infection fecundity and bacterial load was driven by two genotypes that both were derived from line RAL-832. After the experiment was performed, it was discovered that this genotype contains a premature stop codon in the antimicrobial peptide gene, Diptericin. Diptericin is a downstream effector of the IMD pathway, which is responsible for resistance against Gram-negative pathogens. Although traditional life-history theory would suggest that an evolutionary tradeoff may be a result of limited resources between maintenance of the immune system and reproduction, we speculate from our results that it is more likely driven by direct costs of immunity, such as collateral damage from active antimicrobial activity. Other work has demonstrated a tradeoff between immunity and lifespan by showing that constitutive activation of the Gram-negative bacterial recognition protein, PGRP-LE, reduces lifespan. This phenotype can be rescued in flies with a homozygous loss-of-function mutation in the NF-kB transcription factor, Relish . These results, combined with our study, suggest that the downstream effectors are the modulators of these tradeoffs, because if the tradeoff was simply based on resource availability, functional downstream effectors would play a smaller role than recognition and signaling molecules.
We did not observe any costs of tolerance. This suggests that resistance is more costly than tolerance, at least in terms of early-life reproduction. It is possible that under different conditions, increased power, or through measurements of different life-history traits, such as longevity, we may have found a cost of tolerance. Although this is the first attempt to identify costs of tolerance in animals as uninfected fitness, many studies in plants have found costs of tolerance [7, 13], but some have failed to detect these costs [37, 43, 44].
We have empirically demonstrated that defense as tolerance and resistance is a dynamic process with multiple phases of infection, and that these defense strategies are differentially affected by diet and genotype-by-diet interactions. Future theoretical work should aim to incorporate these complexities into disease models, while empirical studies should acknowledge these alternative, yet synergistic, strategies and how they change over time and environment.
Here we show that infection dynamics as well as dietary environment influence estimates of both tolerance and resistance, emphasizing that infection status is not a dichotomous state but a continuum that may yield different defense strategies in different contexts. The presence of genetic variation for fecundity and mortality tolerance is contrary to simple theoretical models, and we show that context dependence and non-independence between defense strategies may be maintaining this variation. The presence of a weak evolutionary cost of resistance, but no costs of tolerance suggests that direct costs of limiting a pathogen burden may be more costly than dealing with the negative consequences of that burden.
Drosophila stocks and diets
Ten lines were randomly chosen from the Drosophila Genetic Reference Panel (DGRP): RAL-26, RAL-217, RAL-272, RAL-318, RAL-370, RAL-374, RAL-595, RAL-732, RAL-832, RAL-897. The lines were collected from the Raleigh, NC, USA farmer’s market in 2003 and inbred with 20 generations of full-sib matings . These lines represent a random snapshot of natural genetic variation. For at least two generations prior to the experiment, all lines were maintained on two diets that varied in the amount of D-glucose. Both diets contained 5% brewer’s yeast w/v, 1% agar, 0.035% phosphoric acid, and 0.42% propionic acid. The low sugar diet contained 2.5% D-glucose, and the high sugar diet contained 5% D-glucose.
Generation of experimental flies
Outbred F1 progeny were derived from the ten DGRP lines using a ring crossing design, such that each line contributed a mother for one experimental genotype and a father for another (see Figure 1). The parental generation was maintained at a low density, approximately 20 females per bottle (30 ml food), and cleared after two days of egg laying to avoid overcrowding effects on offspring. Virgin experimental flies were aged for 3–5 days in groups of twelve flies per vial (8 ml food). For the 24-hour period before infection, females were exposed to males of the same genotype in groups of 12 females and 12 males per vial (Day 0 vial).
The strain of Providencia rettgeri used in this experiment is a Gram-negative extracellular natural pathogen of D. melanogaster. It was isolated from the hemolymph of wild caught D. melanogaster. Overnight cultures were started from a single bacterial colony and were grown overnight in liquid LB at 37°C with shaking. Prior to infections, the overnight culture was diluted in LB to an A600 of 0.2. Flies were infected by pricking in the thorax with a 0.15 mm dissecting pin (Fine Science Tools) dipped in the diluted overnight culture of P. rettgeri. This method delivered approximately 800 bacteria to each fly. Flies were anesthetized on CO2 for 3–4 minutes during infection. Control anesthetized flies were held on CO2 for the same amount of time. Infections were performed over two days for each replicate. The genotype order of infections was determined using a random number generator, with 5 genotypes infected on each of the two days. The infected females were placed in groups of three females per vial and the males were discarded. Approximately 96 females were infected per genotype per diet per experimental replicate.
Post-infection fly handling
Female flies were placed in a fresh ‘Day 1′ vial in groups of three immediately after infection or control handling. The flies were assigned either for measurement of bacterial load or for measurement of fecundity and survival. For each genotype on each diet, there were four uninfected survival/fecundity vials, eight infected survival/fecundity vials and approximately 20 bacterial load vials. For the next five days after infection, all flies were transferred each day and maintained at a density of two to three female flies per vial. The number of surviving flies was recorded daily as the flies in the survival/fecundity treatment were transferred to new vials. These vials were retained and emerging adult offspring were counted for up to 16 days after the transfer. The number of emerged adults was used as the measure of fecundity.
Bacterial load assay
Bacterial load was estimated for each genotype on each diet once a day for five days after infection. Individual females were placed in 500 μl LB and homogenized with a sterile pestle. On Day 1, the homogenates were diluted 1:100 dilution prior to plating. On Days 2 and 3, homogenates were diluted 1:10. No dilution was necessary on Days 4 and 5. These homogenates were then plated onto LB agar using a WASP 2 spiral plater (Microbiology International, Bethesda, MD, USA). Plates were grown overnight at 37°C and resulting colonies were counted using a ProtoCOL plate counting system (Microbiology International) to estimate the number of viable bacteria infecting each fly.
All statistical analyses were performed in R . Mixed-effect models were analyzed using the nlme package . Separate generalized linear models were built for analysis of mortality tolerance, fecundity tolerance, and resistance. The possible factors included in the models were Genotype, Diet, Day Post-Infection, Bacterial Load, and Uninfected Fecundity. Genotype was considered a fixed effect when the biological question related to the existence of genetic variation for a given trait, but genotype was considered a random effect when questions were asked at a species level (e.g. Does diet affect tolerance?). Genotype was used as a random effect in these models, because the question of how diet affects defense treats genotype as a random draw from a population. Linear contrasts were performed using the multcomp package in R . The Tukey’s test was used to compare load, fecundity, and mortality rate for each day. For these comparisons, interaction terms were not included in the models.
Two models were tested to estimate fecundity tolerance. Model A tested for the presence of natural genetic variation in tolerance. The response variable is the infected fecundity corrected for the number of females put into the vial. In order to normalize the distribution of the residuals, all fecundity values were taken to the power of 0.6, which was chosen using the boxcox function in the MASS package in R . The Box-Cox procedure identifies an appropriate exponent to use to transform the data to a normal distribution by calculating the log-likelihood of the data over a range of power transformations. Uninfected Fecundity was included in the models to account for variation in general vigor. Genetic variation for tolerance is indicated by a significant Genotype-by-Load interaction, which shows that certain genotypes are better able to handle the severity of an infection than others. A significant three-way interaction between Genotype, Load, and Diet represents a genotype-by-environment effect on tolerance. For this analysis, the data were split into the acute and chronic phase of infection. Day 1–3 is considered acute phase because that is where most of the mortality and the peak pathogen intensity occur. Day 4–5 is considered the chronic phase of infection because the negative impacts of infection are not as extreme. Model B tested for the effect of diet and day on fecundity tolerance. To correct for correlations that result from repeated measurements on the same group of flies, first-order autoregressive error structure was fit to the model using the corAR1() function from the R package lme4 .
Mortality tolerance was estimated using generalized linear models that followed a binomial distribution and used the number of dead and alive flies as the response variables for each experimental replicate.
Model E tested for genetic variation and a genotype-by-environment effect on resistance, and Model F considered the effects of Diet and Day post-infection on resistance. The response variable in these models was the natural log of the estimated bacterial load.
Correlations between resistance and mortality tolerance or fecundity tolerance were estimated on each day and diet, as well as for an overall estimate for each phenotype determined from the acute stage of the infection (Days 1–3). For this latter correlation, the maximum bacterial load measurement for each genotype was used as the overall estimate of resistance. Fecundity tolerance estimates were least squares mean of infected fecundity standardized by uninfected fecundity and the median bacterial load as determined from Model A. Mortality tolerance was estimated as the proportion of flies surviving three days after infection. Overall estimates of tolerance were estimated from similar models that included all data from Days 1–3.
Drosophila genetic reference panel.
We thank Susan Rottschaefer, Michael Fox, Sarah Short, and Jamilla Akhund-Zade for technical assistance. We thank Moria Chambers, Robin Schwenke, Robert Unckless, Ezra Lencer, and Adam Dobson for helpful comments on the manuscript. We thank Paul Rasmussen and Brooks Miner for statistical advice. This research was supported by NIH Grant R01 AI083932.
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