Complete plastid genome sequences of Drimys, Liriodendron, and Piper: implications for the phylogenetic relationships of magnoliids
© Cai et al; licensee BioMed Central Ltd. 2006
Received: 13 June 2006
Accepted: 04 October 2006
Published: 04 October 2006
The magnoliids with four orders, 19 families, and 8,500 species represent one of the largest clades of early diverging angiosperms. Although several recent angiosperm phylogenetic analyses supported the monophyly of magnoliids and suggested relationships among the orders, the limited number of genes examined resulted in only weak support, and these issues remain controversial. Furthermore, considerable incongruence resulted in phylogenetic reconstructions supporting three different sets of relationships among magnoliids and the two large angiosperm clades, monocots and eudicots. We sequenced the plastid genomes of three magnoliids, Drimys (Canellales), Liriodendron (Magnoliales), and Piper (Piperales), and used these data in combination with 32 other angiosperm plastid genomes to assess phylogenetic relationships among magnoliids and to examine patterns of variation of GC content.
The Drimys, Liriodendron, and Piper plastid genomes are very similar in size at 160,604, 159,886 bp, and 160,624 bp, respectively. Gene content and order are nearly identical to many other unrearranged angiosperm plastid genomes, including Calycanthus, the other published magnoliid genome. Overall GC content ranges from 34–39%, and coding regions have a substantially higher GC content than non-coding regions. Among protein-coding genes, GC content varies by codon position with 1st codon > 2nd codon > 3rd codon, and it varies by functional group with photosynthetic genes having the highest percentage and NADH genes the lowest. Phylogenetic analyses using parsimony and likelihood methods and sequences of 61 protein-coding genes provided strong support for the monophyly of magnoliids and two strongly supported groups were identified, the Canellales/Piperales and the Laurales/Magnoliales. Strong support is reported for monocots and eudicots as sister clades with magnoliids diverging before the monocot-eudicot split. The trees also provided moderate or strong support for the position of Amborella as sister to a clade including all other angiosperms.
Evolutionary comparisons of three new magnoliid plastid genome sequences, combined with other published angiosperm genomes, confirm that GC content is unevenly distributed across the genome by location, codon position, and functional group. Furthermore, phylogenetic analyses provide the strongest support so far for the hypothesis that the magnoliids are sister to a large clade that includes both monocots and eudicots.
Phylogenetic relationships among basal angiosperms have been debated for over 100 years since Darwin  identified the origin and rapid diversification of flowering plants as an "abominable mystery". The difficulty of resolving these relationships is likely due to the rapid radiation of angiosperms. During the past decade there has been considerable interest in estimating phylogenetic relationships based on single or multiple genes to resolve the basal radiation of flowering plants [2–19]. Recently, completely sequenced plastid genomes have been used to estimate relationships among basal angiosperms [9–11, 18, 19] and comprehensive analyses of these data concur with earlier studies resolving either Amborella or Amborella plus the Nymphaelales as the basal-most clade, sister to all other angiosperms [2–8], [12–20]. Although the whole plastid genome approach has enhanced our understanding of basal angiosperm relationships, issues of taxon sampling and methods of phylogenetic analysis have generated considerable controversy regarding the efficacy of this approach [13, 18–23]. One of the major limitations of this approach is the paucity of plastid genome sequences from early diverging lineages, especially the magnoliids, which are currently represented only by Calycanthus .
With four orders, 19 families, and approximately 8,500 species, the magnoliids comprise the largest clade of early diverging angiosperms . Recent single and multigene trees have provided only weak to moderate support for the monophyly of this clade [7, 14, 15, 26–28]. One notable exception is the phylogenetic estimate of Qiu et al.  based on eight plastid, mitochondrial, and nuclear genes and 162 taxa representing all of the major lineages of gymnosperms and angiosperms. This eight-gene tree provides the first strong support for the monophyly of the four orders of magnoliids, and for sister group relationships between the Canellales/Piperales and Laurales/Magnoliales.
One of the most important remaining phylogenetic issues regarding angiosperms is the relationship of magnoliids to the other major clades, including the monocots and eudicots. All three possible relationships among these major angiosperm clades have been generated based on single and multiple gene trees, but none receives strong support in any of these studies [reviewed in ]. The three-gene phylogenetic tree of Soltis et al.  placed the magnoliids and monocots in a clade that was sister to the eudicots. Support for the monophyly of this group, which was referred to as eumagnoliids, was weak with a jackknife value of only 56%. This same topology was generated in Bayesian analyses using the plastid gene matK, with a parsimony bootstrap value of 78% and a Bayesian posterior probability of 0.73 . Two multi-gene molecular phylogenetic studies identified the magnoliids sister to the eudicots. The 11-gene MP trees in Zanis et al.  provided only weak support (56% bootstrap value) for the sister group relationship between magnoliids and eudicots, and in the 9-gene analyses of Qiu et al.  support for this same relationship increased to 78% in ML trees. Finally, a third possible resolution of relationships among magnoliids, monocots, and eudicots was recovered in two other studies based on phytochrome genes  and 17 plastid genes . These studies suggested that magnoliids were sister to a clade that included monocots and eudicots, however, support for this relationship had only weak or moderate bootstrap support (< 50% in Mathews and Donoghue  and 76 or 83% in Graham and Olmstead ). Thus, despite intensive efforts during the past 10 years relationships among magnoliids, monocots, and eudicots remain problematic.
In this paper, we report on the complete sequences of three magnoliid plastid genomes (Drimys, Liriodendron, and Piper). We characterize the organization of two of these genomes, including the most comprehensive comparisons of GC content among completely sequenced plastid genomes. Furthermore, the results of phylogenetic analyses of DNA sequences of 61 genes for 35 taxa, including 33 angiosperms and two gymnosperm outgroups provide new evidence for resolving relationships among angiosperms with an emphasis on relative positions of magnoliids, monocots and eudicots. These results have implications for our understanding of floral evolution in the angiosperms [e.g., ].
Size, gene content, order and organization of the Drimys and Piper plastid genomes
We have sequenced the plastid genomes of three genera of magnoliids, Drimys, Liriodendron, and Piper. In this paper, we only characterize the genome of two of these, Drimys and Piper, and we use 61 protein-coding genes from all three for the phylogenetic analyses. The genome sequence for Liriodendron will be described in a future paper on the utility of 454 sequencing  for plastid genomes (J. E. Carlson et al. in progress).
Comparison of major features of magnoliid plastid genomes
LSC length (bp)
SSC length (bp)
IR length (bp)
Number of genes
Number of gene duplicated in IR
Number of genes with introns (with 2 introns)
Our phylogenetic data set included 61 protein-coding genes for 35 taxa (Table 1), including 33 angiosperms and two gymnosperm outgroups (Pinus and Ginkgo). The data set comprised 45,879 nucleotide positions but when the gaps were excluded there were 39,378 characters.
Taxa included in phylogenetic analyses with GenBank accession numbers and references.
GenBank Accession Numbers
Gymnosperms – Outgroups
Wakasugi et al. 1994 
Leebens-Mack et al 2005 
Goremykin et al. 2003 
Leebens-Mack et al 2005 
Goremykin et al. 2004 
Goremykin et al. 2003 
Leebens-Mack et al 2005 
Hiratsuka et al. 1989 
Chang et al. 2006 
Asano et al. 2004 
Ikeo and Ogihara, unpublished
Leebens-Mack et al 2005 
Leebens-Mack et al 2005 
Maier et al. 1995 
Sato et al. 1999 
Schmitz-Linneweber et al. 2002 
Bausher et al. 2006 
Plader et al. unpublished
Steane 2005 
Saski et al. 2005 
Lee et al. 2006 
Kato et al. 2000 
Lin et al., unpublished
Shinozaki et al. 1986 
Hupfer et al. 2000 
Kim and Lee 2004 
Leebens-Mack et al 2005 
Daniell et al. 2006 
Daniell et al. 2006 
Schmitz-Linneweber et al. 2001 
Jansen et al. 2006 
Relationships among most other major angiosperm clades are congruent in the MP and ML trees. Monophyly of the magnoliids is strongly supported with 92 (MP) or 100% (ML) bootstrap values. Within magnoliids there are two well-supported clades, one including the Canellales/Piperales with 74 (MP) or 99 (ML)% bootstrap support, and a second including the Laurales/Magnoliales with 82 (MP) or 99 (ML)% bootstrap support. The magnoliid clade forms a sister group to a large clade that includes the monocots and eudicots. Support for the sister relationship of magnoliids to the remaining angiosperms is moderate (72% in MP tree, Fig. 8) or strong (99% in ML tree, Fig. 9).
Support for the monophyly of monocots and eudicots is strong with 100% bootstrap values, and relationships among the monocots is identical in both analyses. The Ranunculales occupy the earliest diverging lineage among eudicots, and they are sister to two major, strongly supported clades, the rosids and Caryophyllales/asterids. There is strong support for the sister group relationship between the Caryophyllales and asterids (92% in MP and 100% in ML). Both MP and ML trees provide strong support for the placement of Vitis as the earliest diverging lineage within the rosids. The only remaining incongruence between MP and ML trees is found within the rosids. In both analyses, eurosids I are not monophyletic, although support for relationships among the five representatives of this clade to the eurosid II and Myrtales clades is not strong.
Genome organization and evolution of GC content
The organization of the Drimys and Piper genomes with two copies of an IR separating the SSC and LSC regions is identical to most sequenced angiosperm plastid genomes [reviewed in ]. The sizes of the genome at 160,604 and 160,624 bp, respectively are similar to the each other and Liriodendron, but larger than the only other sequenced magnoliid genome (Calycanthus, 153,337, 23, Table 1). Most of this size difference is due to the larger size of the IR in Drimys (26,649 bp) and Piper (27,039 bp) relative to Calycanthus (23,295 bp), although some is also due to the larger LSC region (Table 1). Expansion and contraction of the IR is a common phenomenon in land plant plastid genomes  with the IR ranging in size from 9,589 bp in the moss Physcomitrella  to 75,741 bp in the highly rearranged angiosperm genome of Pelargonium [36, 37]. Among angiosperms the IR generally ranges in size between 20–27 kb, and the magnoliid genomes except for Calycanthus are at the high end of that range.
Gene order of the magnoliid plastid genomes is identical to tobacco and many other unrearranged angiosperm plastid genomes. There are a few differences in gene content and these can be explained by two phenomena. The first concerns differences in the annotation of two genes in these genomes. Two putative genes (ACRS and ycf15) in Calycanthus were not annotated in Drimys and Piper because several recent studies indicated that they are not functional plastid genes. The sequence of ycf15 has been shown to be highly variable among angiosperms, with conserved motifs at the 5' and 3' ends and an intervening sequence that makes it a pseudogene [38, 39]. An examination of ycf15 transcripts in spinach indicated that is not a functional protein-coding gene . More recent sequence comparisons of ycf15 in other plastid genomes also supported the conclusion that this putative gene is not functional [9, 39]. The ACRS gene was identified by Goremykin et al.  in Calycanthus based on its very high sequence identity with the mitochondrial ACR-toxin sensitivity (ACRS) gene of Citrus jambhiri . This conserved sequence has been identified (as ycf68) in a number of plastid genomes, however, in cases where it has been critically examined the presence of internal stop codons indicates that this is a pseudogene. The second explanation for gene content differences among the three magnoliid genomes is caused by the expansion of the IR in Piper, which results in the duplication of trnH. Small expansions of the IR boundary are common in plastid genomes  resulting in duplications of genes at the IR/SC boundaries. The duplication of trnH in Piper is shared with Nuphar, a member of the Nymphaeales (L. Raubeson et al. unpublished). This expansion of the IR to duplicate trnH has clearly happened independently in Piper and Nuphar since none of the other basal angiosperms or magnoliids have this duplication.
Examination of GC content in 34 seed plant plastid genomes reveals several clear patterns. GC content for the complete genomes ranges between 34–39% (Fig. 3A), confirming previous observations that plastid genomes are in general AT rich [24, 39, 41–47]. The uneven distribution of GC content over the plastid genome is also very evident, and there are several explanations for this pattern. First, there is a clear bias for the coding regions to have a significantly higher GC content than non-coding regions (Figs. 3, 4), which again confirms previous observations based on comparisons of many fewer genomes . Second, there is an uneven distribution of GC content by regions of the genome with the highest GC content in the IR and the lowest in the SSC (Figs. 4, 7). The higher GC content in the IR can be attributed to the presence of the four rRNA genes in this region, which have the highest GC content of any coding regions (Fig. 6B). This higher GC content in the IR region is maintained even when one copy of the IR is lost as in Medicago and Pinus (Fig 7, bottom right panel). The lower GC content in the SSC region is due to the presence of 8 of the 11 NADH genes, which have the lowest GC content of any of the classes of genes compared (Figs. 5, 6). Third, GC content varies by functional groups of genes. Among protein genes, GC content is highest for photosynthetic genes, lowest for NADH genes, with genetic system genes having intermediate values. This same pattern was observed by Shimada and Sugiura  in comparisons of the first three sequenced land plant plastid genomes.
Differences in GC content were also observed by codon position in protein-coding genes (Figs. 3B, 5A,B, 6A). For each of the three classes of genes (photosynthetic, genetic system, and NADH) the third position in the codon has a significant AT bias. This pattern has been observed previously [41, 45, 46, 48], and it has been attributed to codon bias. Previous studies have demonstrated that there is a strong A+T bias in the third codon position for plastid genes [45, 46, 48]. This is in contrast to a GC bias in codon usage for nuclear genes in plants . Several studies have examined codon usage of plastid genes to attempt to determine if these biases can be attributed to nucleotide compositional bias, selection for translational efficiency, or a balance among mutational biases, natural selection, and genetic drift [49–53]. All of these studies have been limited to examining a single or few genes, and they have been constrained by the limited sampling of complete genome sequences for taking variation in GC content into account. Our comparisons of GC content variation for a wide diversity of angiosperm lineages provide a rich source of information for future investigations of the relationship between GC content and codon usage bias.
The debate concerning the identity of the most basal angiosperm lineage continues even though numerous molecular phylogenetic studies of angiosperms have been conducted over the past 15 years [3, 4, 6, 8–11], [14–20], [26–29], [54–66]. Several issues have confounded the resolution of relationships among basal angiosperms, including long branch attraction associated with sparse taxon density and poor taxon sampling, and conflict among trees obtained using different phylogenetic methodologies [4, 6, 7, 11, 13, 18–20, 67]. Most recent studies agree that Amborella and the Nymphaeales represent the earliest diverging angiosperm lineages [3, 4, 6–8], [14–20], [26–29], [57–66]. The most recent multi-gene phylogenetic reconstructions based on nine gene sequences from the plastid, mitochondrial, and nuclear genomes  generate trees supporting each of these two hypotheses depending on the method of phylogenetic analysis and the genes included. Trees generated from plastid genes supported the Amborella basal hypothesis, whereas mitochondrial genes supported the Amborella + Nymphaeales hypothesis. Furthermore, MP analyses tended to support the Amborella basal hypothesis and ML analyses supported Amborella + Nymphaeales. A similar set of relationships was also observed in recent phylogenetic studies using sequences of 61 genes from completely sequenced plastid genomes [18, 19, 67]. In these studies, MP trees placed Amborella alone as the basal most angiosperm with strong support and ML trees placed Amborella + Nymphaeales at the base with moderate support. These differences were attributed to rapid diversification and the lack of extant lineages that could be used to cut the length of branches leading to Amborella and the most recent common ancestor of lineages within the Nymphaeales.
Our phylogenetic analyses include three additional magnoliids from three different orders. Both MP and ML trees (Figs. 8, 9) support Amborella alone as the earliest diverging lineage of angiosperms. Support for this relationship is very strong in MP trees (100% bootstrap) and weak (63%) in ML trees. However, a SH test that constrained Amborella + Nymphaeales in a basal position indicated that the two hypotheses of basal angiosperm relationships are not significantly different. Thus, although both MP and ML analyses including the three additional magnoliid taxa support Amborella as the basal-most branch in the angiosperm phylogeny, sampling of more taxa and genes, and further investigations of model specification in phylogenetic analyses are needed before this issue is fully resolved [see discussion in [18, 23]].
Several earlier molecular phylogenetic studies based on one or a few genes [27, 56, 63] did not support the monophyly of magnoliids. Furthermore, morphological studies of angiosperms failed to detect any synapomorphies for this group. The circumscription, monophyly, and relationships of magnoliids has only recently been established based on phylogenetic analyses of multiple genes [14, 15]. These earlier multigene trees provided only weak to moderate support for the monophyly of magnoliids and the sister group relationships of the Canellales/Piperales and Laurales/Magnoliales. A recent study using eight plastid, mitochondrial, and nuclear genes  provided the first strong support for both the monophyly and relationships among the four orders of magnoliids. Our phylogenetic trees based on 61 plastid protein-coding genes also provide strong support for the monophyly of magnoliids and the sister relationship between the Canellales/Piperales and Laurales/Magnoliales.
One of the most controversial remaining issues regarding relationships among angiosperms concerns the resolution of relationships among the magnoliids, monocots and eudicots. Previous phylogenetic studies have supported three different hypotheses of relationships among these lineages: (1) (magnoliids (monocots, eudicots)), (2) (monocots (magnoliids, eudicots)), and (3) (eudicots (magnoliids, monocots)). The first hypothesis was supported in phylogenetic analyses based on phytochrome genes  and 17 plastid genes  but bootstrap support for a sister relationship of monocots and eudicots was only 67%. Several studies supported the second hypothesis [7, 16, 68], however, bootstrap support was again weak ranging from 55 – 78%. The three-gene phylogenetic tree of Soltis et al.  supported the third hypothesis with only 56% jackknife support. This relationship was also recovered in a matK gene tree with a parsimony bootstrap value of 78% and a posterior probability of 0.73 . Both MP and ML trees based on 61 plastid-encoded protein genes support hypothesis 1 (Figs. 8, 9). Branch support for this hypothesis is moderate (MP, Fig. 8) or strong (ML, Fig. 9). Congruence of the results from both MP and ML analyses is notable because our previous phylogenetic analyses using whole plastid genomes that included only one member of the magnoliid clade (Calycanthus, [18, 67]) were incongruent. In these earlier studies, MP trees supported hypothesis 2 (monocots sister to a clade that included magnoliids and eudicots), whereas ML trees supported hypothesis 1 (magnoliids sister to a clade that included monocots and dicots). These differences provide yet another example of the importance of expanded taxon sampling in phylogenetic studies using sequences from whole plastid genomes [18, 67]. The addition of other angiosperm lineages, especially members of the Chloranthales, Certatophyllaceae, and Illiciales may be critical for providing additional resolution of relationships among the major clades.
The genome sequences of three additional magnoliids have a very similar size and organization to the ancestral angiosperm plastid genome. Comparisons of 34 seed plant plastid genomes confirm that GC content is unevenly distributed across the genome by location, codon position, and functional group. Phylogenetic analyses for 61 protein-coding genes using both maximum parsimony and maximum likelihood methods for 35 seed plants provide moderate or strong support for the placement of Amborella sister to all other angiosperms. Furthermore, there is strong support for the monophyly of magnoliids and for the recognition of two major clades, the Canellales/Piperales and the Laurales/Magnoliales. Finally, phylogenetic analyses provide the strongest support so far that magnoliids are sister to a large clade that includes both monocots and eudicots.
Plastid isolation, amplification, and sequencing
10–20 g of fresh leaf material of Drimys granatenis and Piper coenoclatum was used for the plastid isolation. Leaf material was obtained from the University of Connecticut Greenhouses (accession numbers 200100052 for Drimys and 199600027 for Piper). Plastids were isolated from fresh leaves using the sucrose-gradient method . They were then lysed and the entire plastid genome was amplified using Rolling Circular Amplification (RCA, using the REPLI-g™ whole genome amplification kit, Molecular Staging) following the methods outlined in Jansen et al. . The RCA product was then digested with the restriction enzymes Eco RI and Bst BI and the resulting fragments were separated by agarose gel electrophoresis to determine the quality of plastid DNA. The RCA product was sheared by serial passage through a narrow aperture using a Hydroshear device (Gene Machines), and the resulting fragments were enzymatically repaired to blunt ends and gel purified, then ligated into pUC18 plasmids. The clones were introduced into E. coli by electroporation, plated onto nutrient agar with antibiotic selection, and grown overnight. Colonies were randomly selected and robotically processed through RCA of plasmid clones, sequencing reactions using BigDye chemistry (Applied Biosystems), reaction cleanup using solid-phase reversible immobilization, and sequencing using an ABI 3730 XL automated DNA sequencer. Detailed protocols are available at .
Genome assembly and annotation
Drimys and Piper. Sequences from randomly chosen clones were processed using PHRED and assembled based on overlapping sequence into a draft genome sequence using PHRAP . Quality of the sequence and assembly was verified using Consed . In most regions of the genomes we had 6–12-fold coverage but there were a few areas with gaps or low depth of coverage. PCR and sequencing at the University of Texas at Austin were used to bridge gaps and fill in areas of low coverage in the genome. Additional sequences were added until a completely contiguous consensus was created representing the entire plastid genome with a minimum of 2X coverage and a consensus quality score of Q40 or greater.
Liriodendron. The Liriodendron plastid genome sequence was obtained using 454 sequencing technology . This work will be described more fully in a separate paper focused in the utility of 454 technology for plastid genome sequencing (J. E. Carlson et al. in progress). Briefly, plastid genome containing clones were identified from a genomic bacterial artificial chromosome (BAC) library that had been constructed for Liriodendron . The entire plastid genome was sequenced from a pooled sample of DNAs isolated from three overlapping large-insert BAC clones. The BAC DNAs were processed for 454 sequencing according to protocols provided by 454 Life Sciences (Branford, CT, also see ). One-half plate run on the Genome Sequencer 20™ System (Life Sciences, Branford, CT; Roche Applied Science, Indianapolis, IN) generated over 15,000 sequences with an average read length of 103 bp. Reads including vector sequence were removed and the remaining reads were assembled using the 454-de novo assembly software. Each base in the assembly was inferred based on an average of 90 independent reads.
The coordinate of each genome was standardized for gene annotation to be the first bp after IRa on the psbA side. The genomes were annotated using the program DOGMA (Dual Organellar GenoMe Annotator ). All genes, rRNAs, and tRNAs were identified using the plastid/bacterial genetic code.
Examination of GC content
GC content was calculated for 34 seed plant plastid genomes, including the gymnosperm Pinus and 33 angiosperms. GC content was also determined for 66 protein-coding genes. These genes were partitioned into three functional groups (photosynthesis (33), genetic system genes (22), and NADH (11) genes), and GC content was calculated for the entire gene and the first, second, and third codon positions. The genes included in each of these three groups are: (1) photosynthetic genes (atpA, atpB, atpE, atpF, atpH, atpI, psbZ, petA, petB, petD, petG, petL, petN, psaA, psaB, psaC, psaI, psaJ, psbA, psbB, psbC, psbD, psbE, psbF, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, rbcL); (2) genetic system genes (rpl14, rpl16, rpl2, rpl20, rpl32, rpl33, rpl36, rpoA, rpoB, rpoC1, rpoC2, rps11, rps12, rps14, rps15, rps18, rps19, rps2, rps3, rps4, rps7, rps8); and (3) NADH genes (ndhA, ndhB, ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK). GC content was also plotted over the entire genome for all 34 taxa, which were classified into 10 groups based on gene order and phylogenetic placement (Figs. 8, 9).
Statistical analyses of GC content between rRNA genes and complete genomes and between 72 protein-coding genes and complete genomes were performed using a t test. Analyses of GC content among the three codon positions and among the three functional classes were performed using one way ANOVA. All statistical analyses used the program SPSS, version 9.0 .
The 61 protein-coding genes included in the analyses of Goremykin et al. [9, 10] and Leebens-Mack et al.  were extracted from Drimys, Liriodendron and Piper using the organellar genome annotation program DOGMA . The same set of 61 genes was extracted from plastid genome sequences of 32 other sequenced genomes (see Table 1 for complete list). All 61 protein-coding genes of the 35 taxa were translated into amino acid sequences, which were aligned using MUSCLE  followed by manual adjustments, and then nucleotide sequences of these genes were aligned by constraining them to the aligned amino acid sequences. A Nexus file with character sets for phylogenetic analyses was generated after nucleotide sequence alignment was completed. The complete nucleotide alignment is deposited online at .
Phylogenetic analyses using maximum parsimony (MP) and maximum likelihood (ML) were performed using PAUP* version 4.0b10  on a data including 35 taxa (Table 1). Phylogenetic analyses excluded gap regions. All MP searches included 100 random addition replicates and TBR branch swapping with the Multrees option. Modeltest 3.7  was used to determine the most appropriate model of DNA sequence evolution for the combined 61-gene dataset. Hierarchical likelihood ratio tests and the Akaikle information criterion were used to assess which of the 56 models best fit the data, which was determined to be GTR + I + Γ by both criteria. For ML analyses we performed an initial parsimony search with 100 random addition sequence replicates and TBR branch swapping, which resulted in a single tree. Model parameters were optimized onto the parsimony tree. We fixed these parameters and performed a ML analysis with three random addition sequence replicates and TBR branch swapping. The resulting ML tree was used to re-optimize model parameters, which then were fixed for another ML search with three random addition sequence replicates and TBR branch swapping. This successive approximation procedure was repeated until the same tree topology and model parameters were recovered in multiple, consecutive iterations. This tree was accepted as the final ML tree (Fig. 9). Successive approximation has been shown to perform as well as full-optimization analyses for a number of empirical and simulated datasets . Non-parametric bootstrap analyses  were performed for MP analyses with 1000 replicates with TBR branch swapping, 1 random addition replicate, and the Multrees option and for ML analyses with 100 replicates with NNI branch swapping, 1 random addition replicate, and the Multrees option.
Test of alternate topology
A Shimodaira-Hasegawa (SH) test  was performed to determine if the alternative topology with Amborella + Nymphaeales basal was significantly worse than the ML tree that places Amborella alone as the basal angiosperm lineage. A constraint topology with this alternative tree topology was used and the SH test was conducted using RELL optimization  as implemented in PAUP* version 4.0b10 .
small single copy
large single copy, bp, base pair
hypothetical chloroplast reading frame
We thank Clinton Morse from the University of Connecticut greenhouses for providing leaf material of Drimys and Piper. We also thank Andrew Alverson for assistance with the S-H tests. This research was supported in part by a grant from the National Science Foundation (DEB 0120709) and the Sidney F. and Doris Blake Centennial Professorship in Systematic Botany to RKJ. Part of this work was performed under the auspices of the U.S. Department of Energy, Office of Biological and Environmental Research, by the University of California, Lawrence Berkeley National Laboratory, under contract No. DE-AC02-05CH11231.
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