Evolutionary genomic remodelling of the human 4q subtelomere (4q35.2)
© Bodega et al; licensee BioMed Central Ltd. 2007
Received: 24 November 2006
Accepted: 14 March 2007
Published: 14 March 2007
In order to obtain insights into the functionality of the human 4q35.2 domain harbouring the facioscapulohumeral muscular dystrophy (FSHD) locus, we investigated in African apes genomic and chromatin organisations, and the nuclear topology of orthologous regions.
A basic block consisting of short D4Z4 arrays (10–15 repeats), 4q35.2 specific sequences, and approximately 35 kb of interspersed repeats from different LINE subfamilies was repeated at least twice in the gorilla 4qter. This genomic organisation has undergone evolutionary remodelling, leading to the single representation of both the D4Z4 array and LINE block in chimpanzee, and the loss of the LINE block in humans. The genomic remodelling has had an impact on 4qter chromatin organisation, but not its interphase nuclear topology. In comparison with humans, African apes show very low or undetectable levels of FRG1 and FRG2 histone 4 acetylation and gene transcription, although histone deacetylase inhibition restores gene transcription to levels comparable with those of human cells, thus indicating that the 4qter region is capable of acquiring a more open chromatin structure. Conversely, as in humans, the 4qter region in African apes has a very peripheral nuclear localisation.
The 4q subtelomere has undergone substantial genomic changes during evolution that have had an impact on chromatin condensation and the region's transcriptional regulation. Consequently, the 4qter genes in African apes and humans seem to be subjected to a different strategy of regulation in which LINE and D4Z4 sequences may play a pivotal role. However, the effect of peripheral nuclear anchoring of 4qter on these regulation mechanisms is still unclear. The observed differences in the regulation of 4qter gene expression between African apes and humans suggest that the human 4q35.2 locus has acquired a novel functional relevance.
The distal portion of the human 4q35 genomic region (4q35.2) contains a complex arrangement of repetitive sequences and several genes including facioscapulohumeral muscular dystrophy (FSHD) region genes 1 and 2 (FRG1 and FRG2) . A polymorphic tandem array of 3.3 kb repeats (D4Z4) has also been detected distally to these genes ; the D4Z4 unit contains an open reading frame (ORF) encoding a homeobox protein (DUX4), but DUX4 transcription has not been demonstrated [3, 4].
Deletions within the subtelomeric D4Z4 array lead to autosomal-dominant facioscapulohumeral muscular dystrophy (FSHD MIM 158900) [2, 5]. Unaffected individuals have 11–100 copies of the D4Z4 repeat on both chromosomes 4q, whereas almost all FSHD patients have one chromosome 4q characterised by 1–10 tandem copies . Moreover, 4q35.2 D4Z4 repeats are methylated in the general population, whereas the contracted array is hypomethylated in FSHD patients [6, 7]. The property of 4qter array methylation has led to the hypothesis that the pathogenesis of FSHD is associated with an epigenetic mechanism. In this regard, the array contraction might cause proximal or distal changes in chromatin structure, with the consequent up-regulation of one or more 4qter genes , and it is known that transgenic mice over-expressing the FRG1 gene (located 125 kb from the D4Z4 array) develop a muscular dystrophy resembling human FSHD.
Some studies of inappropriate gene activation in the FSHD region support this hypothesis [9–11], but other papers report a similar level of 4qter gene expression in FSHD patients and controls [12, 13]. Furthermore, recent findings indicate that FSHD may share some features with nuclear envelope dystrophies [14–16]. Unlike most other chromosome ends, normal and FSHD 4qter alleles are preferentially localised at the nuclear rim [14, 15]. It is assumed that this consistent and non-random localisation of 4qter in the periphery of human cell nuclei has some functional significance, and that FSHD may be due to improper interactions with transcription factors or chromatin modifiers at the nuclear envelope .
Despite these observations, studies aimed at defining the molecular mechanisms underlying the pathogenesis of FSHD are difficult to perform because the genomic sequences involved in the disease are a complex patchwork of duplications. The D4Z4 repeat is not restricted to chromosome 4; perfect arrays of D4Z4 units can also be detected on chromosome 10q, and there are additional homologous sequences interspersed with beta satellites on many heterochromatic loci, such as the short arms of acrocentric chromosomes and the pericentromeric region of chromosome 1q . Moreover, a subset of 4q35.2 sequences proximal and distal to the D4Z4 array, including the FRG1 and FRG2 genes, are duplicated in the human genome [4, 18, 19]. This complex genomic scenario makes it difficult to understand the regulation of gene expression at 4q35.2, as well as its alteration in FSHD.
In order to obtain further insights into the function of the human 4qter genomic region, we studied its evolution by investigating genomic organisation, nuclear positioning, chromatin acetylation levels and gene expression in African apes. We chose the gorilla as a starting point because previous studies have indicated the presence of a chromosome-specific block of subtelomeric sequences in 4qter , and D4Z4 dispersion is less complex than in humans.
Isolation and sequence characterisation of gorilla 4qter genomic clones
A gorilla genomic library was screened using a probe from a human subtelomeric block of sequences (defined as block 3 in ) that are located on chromosomes 1p, 8q, 15q and 19p in the human genome, but only on chromosome 4qter in gorilla and chimpanzee. A non-repetitive hybridisation probe was generated by PCR on DNA from BAC AC140725 mapped to 15q26.3, which includes subtelomeric block 3  (Additional file 1). By this approach, six BAC clones were obtained. In the human reference sequence, one or both ends of five clones (CH255-11C6, CH255-18C5, CH255-23B19, CH255-39M12, and CH255-41H7) were similar within a region of approximately 35 kb on chromosomes 15q26.3 and 19p13.3  (Additional file 1 and Additional file 2), which proved to be a complex patchwork of fragments from different LINE subfamilies (the LINE block) (Additional file 3). Subtelomeric block 3 and the LINE block in the human reference sequence overlap for ~10 kb (Additional file 1). Furthermore, one end of three clones (CH255-23B19, CH255-39M12 and CH255-39N14) showed similarity with the 4q35.2 locus, within a region of approximately 15 kb distal to the FRG2 gene (Additional file 2).
After KpnI or EcoRI digestion and hybridisation with D4Z4 (LSau probe), all of the clones showed a very similar restriction and hybridisation pattern of bands (Additional file 4). These results strongly suggested that all of the isolated BACs carry very similar DNA sequences, including an array of approximately 10–15 D4Z4 repetition units, as derived from the densitometric profile of KpnI-digested BAC DNAs.
The sequence content of the gorilla BAC clones was investigated by means of orthologous PCR using primer pairs derived from the human reference sequence that defined the FRG1 and FRG2 promoters and marker 13E11 at 4q35.2, and 35 kb of the LINE block at 15q26.3 (the primer pairs are listed in Additional file 5). All of the clones were remarkably similar insofar as they included the FRG2 promoter, the 13E11 marker, and an almost complete LINE block of 35 kb (Additional file 6). The isolated gorilla genomic clones thus showed redundancy of essentially three classes of sequences: an array of D4Z4 repeats, a LINE block, and a region of non-repetitive 4q35.2 DNA between the FRG2 gene and the 13E11 marker (approx. 40 kb). Taken together, the molecular analyses strongly suggested that the isolated genomic sectors genuinely derived from the gorilla 4q35.2 locus.
Chromosomal evolution of the 4qter region
The chromosomal distribution of D4Z4 and the LINE block was derived using probes consisting of a 3.3 kb unit (39M12-D4Z4) and a LINE fragment of ~10 kb (39M12-LINE) obtained from BAC CH255-39M12. The LINE probe hybridised only the gorilla and chimpanzee 4qter region, whereas four subtelomeric regions were highlighted on human chromosomes (1p, 8p, 15q and 19p) (Fig. 1 and Additional file 7). Conversely, the D4Z4 repeats showed a more pronounced spread from gorilla to humans (Fig. 1 and Additional file 7).
Genomic organisation of the gorilla 4qter region
Nuclear topology of the 4qter region in African apes
Visual inspection of 3D image stacks from 29 gorilla nuclei revealed 36% of 4qter signal loci abutted to the nuclear envelope defined by the border of nuclear counterstaining (Fig. 3A). One allele in 7/29 cells (~24%), and both alleles in a further seven cells (~24%), were found at the nuclear edge (Fig. 3A). In addition, 10/29 nuclei showed multiple localised hybridisation signals for one allele per nucleus, which were clearly distinguishable from the double-dot BAC signals in replicated loci (Fig. 3B). Visual inspection of confocal image stacks from 28 chimpanzee nuclei identified one allele at the nuclear edge in 11/28 cells (~39%) and both alleles at the nuclear edge in 4/28 cells (~14%): a total of 34% of alleles (Fig. 3C). All of the BAC signals were dot-shaped; there were no multiple localised hybridization signals like those seen in the gorilla nuclei.
As in gorilla, quantitative evaluation revealed the clearly peripheral nuclear localisation of the chimpanzee 4qter (Fig. 4). The ARR for 28 evaluated nuclei was 78.3%, corresponding to a mean distance of 510 nm from the nuclear edge (Fig. 4A and 4B). Furthermore, as in gorilla, the 4qter region was significantly more peripheral than the chromosome 4 territory (P = 0.020 and P = 0.001). Notably, the distribution curve obtained using 3DRRD software revealed two peaks in the nuclear periphery at approximately 75% ARR and 90% ARR, thus indicating two distinct preferential locations for the BAC signals. With respect to the chromosome 4 territory surface, the BAC clone was preferentially located at the surface or outside the "core" territory (an average of 60 nm outside) (Fig. 4C).
Chromatin organisation and gene expression of the 4qter region in African apes and humans
Expression analysis of the FRG1 and FRG2 genes by means of quantitative RT-PCR confirmed the results of the ChIP analysis: the FRG1 and FRG2 genes were significantly (75%) less expressed in gorillas and chimpanzees than in humans (Fig. 5C). However, treatment with the histone deacetylase inhibitor Trichostatin A (TSA) increased both histone H4 acetylation, and FRG1 and FRG2 expression, in gorilla and chimpanzee cells to levels that were comparable with those observed in human cells (Fig. 5B and 5C). This may indicate the presence of repressive complexes in the gorilla and chimpanzee 4q subtelomere that are absent from the human orthologous region, an interpretation that is supported by the analysis of FRG1 and FRG2 gene expression in TSA-treated human cells (Fig. 5C).
In this study, we investigated in African apes the genomic and chromatin organisation of regions syntenic to the human chromosome 4q35.2 harbouring the FSHD locus, concentrating on the D4Z4 array that has been previously suggested to be the pivotal sequence motif for human 4q35.2 transcriptional regulation . As most hominoid species show considerable trans-chromosomal duplication and spreading of D4Z4 repeats, we analysed the gorilla species because it has only a few D4Z4 chromosomal locations .
Genomic analysis of the gorilla locus syntenic to human chromosome 4q35.2 revealed clear differences in organisation in comparison with humans. A basic block of short D4Z4 arrays (10–15 repeats) and 4q35.2-specific sequences (i.e. the FRG2 gene and the 13E11 marker) spaced by LINE is repeated at least twice in gorilla, whereas chimpanzee has a single D4Z4 array linked to a LINE block, and humans show further remodelling involving the loss of the LINE block. The 4q subtelomere therefore underwent substantial genomic changes during evolution, and its remodelling essentially involved D4Z4 and LINE sequences (see Fig. 2C).
In humans, the LINE block is still present at the subtelomere of chromosomes 1p, 8p, 15q and 19p, which suggests that at least the first round of duplications preceded its deletion at 4q35.2. This was a recent evolutionary event that might be mediated by the instability of subtelomeric regions, which are well known to be prone to reciprocal translocations [20, 23], whereas the dispersal of the D4Z4 array from the 4q ancestral locus of the macaque has an older evolutionary history, as it took place in an ancestor of the orang-utan and preferentially involved acrocentric chromosomes in both chimpanzee and man [4, 17, 24]. Furthermore, 1qcen, 10qcen and 10qter are human-specific sequence dispersals of D4Z4 repeats.
Although the sequence dispersal of D4Z4 repeats during evolution preceded that of the LINE block, their simultaneous occurrence at gorilla and chimpanzee 4qter may explain the evolutionary remodelling of the region. Both sequence types are prone to unequal homologous recombinations leading to the expansion/contraction of the 4q subtelomere, and this mechanism could account for the expansion of the D4Z4 array and the complete loss of the LINE block in man.
Despite the evolutionary genomic remodelling of the 4q subtelomere, both gorilla and chimpanzee lymphoblastoid cells showed very little or undetectable histone H4 acetylation at the 13E11 marker, and the FRG1 and FRG2 gene promoters; furthermore, this was paralleled by very low levels of FRG1 and FRG2 mRNA expression. Conversely, human lymphoblastoid cells showed higher levels of both FRG1 and FRG2 promoter acetylation and mRNA expression.
The human cell data partially agreed with the results of previous experiments comparing histone H4 acetylation in lymphoblastoid and fibroblast cells from normal individuals and FSHD patients, which led to the conclusion that the observed acetylation levels across the 4q35.2 region were not like those in condensed chromatin . Therefore, the low acetylation levels in gorilla and chimpanzee cells indicate the occurrence of a more condensed chromatin structure at 4qter, and this correlates well with their very low expression of FRG1 and FRG2 genes.
However, the inhibition of histone deacetylase activity in African apes restored the expression of FRG1 and FRG2 to levels comparable with those found in human cells, and this reversibility of histone acetylation makes it possible to speculate that the 4q subtelomeric region in certain gorilla and chimpanzee cell differentiation lineages could acquire a more open chromatin structure. Within the gorilla and chimpanzee locus syntenic to human chromosome 4q35.2, LINE may represent candidate sequences for chromatin condensation as the silencing of gene expression has been associated with an epigenetic mechanism mediated by long and short interspersed repeats . In this regard, LINE enrichment has been correlated with the spread of X chromosome inactivation .
Previous studies of normal and FSHD human nuclei have shown that the 4q subtelomere is consistently localised at the nuclear periphery [14, 15]. A similar analysis of 3D-preserved chimpanzee and gorilla fibroblast nuclei showed a considerable evolutionary conservation of the nuclear positioning of the chromosome 4 territory and 4q subtelomere. We found that, as in human fibroblast nuclei [14, 15, 27], the chromosome 4 territory in chimpanzee and gorilla is located in the nuclear periphery, and the locus syntenic to human chromosome 4q35.2 has an even more peripheral location near or at the nuclear edge. This extremely peripheral location cannot be simply explained by the current hypothesis suggesting that local gene density determines the nuclear positioning of a locus , because the local gene density of the 4qter region (7.6 genes/Mb in a 3 Mb window, build 36.2 ) is higher than that of the entire chromosome territory (6.00 genes/Mb).
In addition to the remarkable similarities in the spatial arrangement of the 4q subtelomere in humans and African apes, we also found some notable qualitative differences in the topology of chimpanzee and gorilla nuclei. In particular, the 4q subtelomere in chimpanzee nuclei showed two peripheral but distinctly different preferential positions, which may indicate the occurrence of 4q allelic variants .
Localization to the nuclear periphery is generally associated with gene repression , and the condensed chromatin structure and low expression of 4qter genes in gorilla and chimpanzee are in line with this. Conversely, although maintaining the preferential association of the 4q35.2 locus with the nuclear periphery, human cells show less chromatin condensation and higher 4q35.2 gene expression. On the basis of these data, we hypothesise that the peripheral localisation of the 4qter region is maintained independently of: a) the underlying DNA sequences (i.e. D4Z4 and LINE), b) their genomic organisation; and c) the expression of embedded genes. However, the evolutionary conservation of 4qter nuclear topology suggests that still unidentified subtelomeric DNA sequences are responsible for the preferential nuclear location, or still unknown epigenomic features of the nuclear periphery play a role in the sub-localisation of 4qter in African apes and humans.
The chromatin condensation and nuclear anchoring of the 4q subtelomere in gorilla and chimpanzee probably reflect a need to repress genes actively in a lineage-dependent manner, and sequester them in a particular nuclear sub-compartment capable of maintaining the repressive state. The LINE-mediated heterochromatisation of the African ape 4qter region could be triggered in trans by interaction with constitutive heterochromatin-associated proteins residing at the inner nuclear membrane .
In human cells, the genes of the FSHD region could be protected from repression by the loss of the LINE block in association with the expansion of the D4Z4 array and, in this regard, it has been suggested that D4Z4 may be a barrier or insulator against heterochromatin-mediated gene silencing [22, 31]. Interestingly, mutations in the lamin A-type gene (LMNA) that cause human diseases called "laminopathies" can also abrogate the nuclear peripheral location of the 4q subtelomere , and so 4q35.2 gene expression might be dictated by the interaction of chromatin and/or DNA with components lining the inner nuclear membrane, such as the lamins known to recruit transcriptional regulators . However, it is still not possible to rule out the possibility that repressive histone marks are responsible for the fine-tuning of human 4q35.2 gene expression.
Our study showed a particular genomic organisation of the gorilla locus syntenic to human chromosome 4q35.2. During evolution, this chromosome region has undergone extensive remodelling in both chimpanzees and humans. Particularly in human cells, 4q35.2 genomic remodelling had an impact on chromatin condensation and the regulation of FRG1 and FRG2 gene expression, but did not influence the peripheral nuclear positioning of 4qter. These data suggest that evolutionary remodelling has probably conferred a novel functional relevance to the human 4q35.2 locus, and reinforce the idea that most biological phenomena can be better understood if studied in an evolutionary frame.
Genomic library screening
The genomic library was obtained from BAC/PAC Resources, Children's Hospital, Oakland Research Institute (CHORI-255: Gorilla BAC Library). High-density arrayed BAC filters were hybridised according to the instructions provided by BAC/PAC Resources  with a 32P-labelled non-repetitive probe obtained by means of PCR (for primers, see Additional file 5) from human BAC clone AC140725 mapping to 15q26.3 and containing subtelomeric block 3  after repeat masking ; the filter was also hybridised with the human LSau probe pA1 , and the genomic clones positive to both probes were selected.
DNA isolation and Southern blot hybridisation
The BAC clones were grown in LB medium supplemented with chloramphenicol. The DNA was purified using a Sigma PhasePrep BAC kit (Sigma Aldrich, USA), digested with restriction enzymes (Biolabs, USA), fractionated by means of agarose gel electrophoresis, blotted onto nylon filters (Hybond N+, Amersham, UK), and hybridised with a 32P-labelled probe representative of D4Z4 (pA1) and beta satellite sequences (A17a plasmid) . Molecular hybridisation was performed in 2 × SSC at 60°C overnight, and the filters were washed at 60°C in 1 × SSC twice for 20 minutes. The hybridisation signals were quantified by means of phosphoimaging (Typhoon 9200, Amersham).
Polymerase chain reaction, cloning and sequencing
Polymerase chain reactions were carried out using the primer pairs listed in Additional file 5, and respectively derived from the human genomic 4q35.2 region and the sequenced GGO BAC ends showing similarities with the human subtelomeric LINE block. The PCR mixtures of 25 μl included 100 ng of DNA, 0.4 μM of each primer, 0.2 mM dNTP mix, and 1 U of Red Taq DNA polymerase in standard reaction buffer (Sigma Aldrich, USA). The PCR conditions generally consisted of one preheating cycle at 95°C for 3 min, followed by 30 cycles of denaturation at 95°C for 30–60 s, annealing at 46°C–65°C for 30 s, and extension at 72°C for 30 s – 2 min. Inter-Alu PCR was carried out as previously described . For long PCR amplification, a 50 μl reaction mixture was used according to the manufacturer's instruction (Roche, Taq Expand 20 kb), and the primers were designed on gorilla BAC end sequences (Additional file 5). Amplification was performed under the following conditions: 2 min at 92°C for initial denaturation, followed by 10 cycles of 10 s at 92°C and 20 min at 68°C, and an additional 20 cycles of 10 s at 92°C, 20 min with autoextension of 10 s per cycle at 68°C, and 7 min at 68°C for final elongation. A D4Z4 unit was sub-cloned in BSK vector from BAC CH255-39M12 after KpnI digestion and completely sequenced using the primers shown in Additional file 5 and a Big Dye terminator 3.1 system (Perkin Elmer Applied Biosystems, Norwalk, Conn) following the manufacturer's instructions. The nucleotide sequence is available at , with accession number: ED549314.
T7/Sp6 BAC ends were sequenced using the Big Dye terminator 3.1 system as described by BAC/PAC Resources . All of the fluorescent traces were analysed using the Applied Biosystem Model 3100 DNA Sequencing System (Applied Biosystem). DNA sequence analysis was performed using DNASTAR software and NCBI facilities . The newly derived nucleotide sequences are available at  with accession numbers: ED549315 (CH255-11C6/Sp6), ED549316 (CH255-11C6/T7), ED549317 (CH255-18C5/Sp6), ED549318 (CH255-18C5/T7), ED549319 (CH255-23B19/Sp6), ED549320 (CH255-23B19/T7), ED549321 (CH255-39M12/Sp6), ED549322 (CH255-39M12/T7), ED549323 (CH255-39N14/Sp6), ED549324 (CH255-39N14/T7), ED549325 (CH255-41H7/Sp6), ED549326 (CH255-41H7/T7).
Fluorescent in situ hybridisation (FISH) on metaphase chromosome spreads
The metaphase chromosome spreads were obtained by standard methods from peripheral blood lymphocytes of normal human (HSA) donors, and from the primate lymphoblastoid or fibroblast cell lines Gorilla gorilla (GGO) and Pan troglodytes (PTR). The primate cell lines were provided by M. Rocchi . The probes were labelled with digoxigenin-dUTP or biotin-dUTP (Roche Diagnostic) using a nick translation kit (Roche Diagnostic), and detected using fluorescein-streptavidin (SIGMA) for the biotin-labelled probes, and a CY3-anti-digoxigenin conjugated antibody (Jackson ImmunoResearch Laboratories) for the digoxigenin-labelled probes. The chromosomes were counterstained with propidium iodide and DAPI in antifade (Vectashild), and then visualised using a Leitz DM-RB microscope equipped for DAPI and FITC/TRITC epifluorescence optics. Hybridisations were performed in 50% formamide (v/v), 10% dextran sulfate, 2 × SSC at 37°C, in the presence of human Cot1 DNA (Gibco-BRL). Post-hybridisation washing included 50% formamide, 2 × SSC at 42°C, followed by three washes in 1 × SSC at 60°C (HSA), or 50% formamide, 1 × SSC at 37°C, followed by three washes in 1 × SSC at 42°C (GGO and PTR). The chromosomes were stained with DAPI (4',6-diamidino-2-phenylindole), and digital images were captured using a Leica DMRXA epifluorescence microscope equipped with a cooled CCD camera (Princeton Instruments). The fluorescence signals were recorded separately as gray-scale images. The images were pseudocoloured and merged using Adobe Photoshop software.
Stretched chromosome preparation
The chromosomes were mechanically stretched as previously described . Aliquots of 103–106 mitotically active lymphoblast cells were washed in PBS and resuspended in the hypotonic solution (10 mM Hepes, 30 mM glycerol, 1.0 mM CaCl2 and 0.8 mM MgCl2) for ten minutes. Aliquots of 0.5 ml of the hypotonic cell suspensions were centrifuged (Cytospin 2, Shandom) onto clean glasses at 800 rpm for four minutes, and fixed in -20°C methanol for 30 minutes.
FISH to 3D-preserved interphase nuclei
The gorilla (Gorilla gorilla) and bonobo (Pan paniscus) fibroblast cell lines with normal diploid karyotypes (data not shown) were kindly provided by W. Schempp, University of Freiburg, Germany. The protocol for the 3D-FISH experiments has been previously described . The cells were fixed in 4% paraformaldehyde (PFA) in 1 × PBS for 10 min. The permeabilisation steps included treatment with 0.5% Triton-X100 (20 min), 20% glycerol in PBS for at least one hr, repeated freeze/thawing in liquid nitrogen, and incubation in 0.1 N HCl (5 min) and pepsin solution (2.5 mg/ml pepsin in 0.01 N HCL at 37°C for 5–10 min). The human chromosome 4 painting probe was labelled with DOP-PCR in the presence of TAMRA-dUTP, and the BAC clone 39M12 with Biotin-dUTP (Roche) by nick-translation, and 400 ng-2 μg of both probes were mixed with a 10-fold excess of human Cot-1 DNA. In situ hybridisation was performed for 48 hours, followed by stringency washes for 3 × 5 min in 0.1 × SSC (60°C). The biotinylated probe was detected with Avidin-Alexa488 (Molecular Probes). The 3D-fixed nuclei were counterstained for 5 min with 1 μM ToPro-3 (Molecular Probes), and visualised using a three-channel laser scanning confocal microscope (Leica SP-1; Leica Microsystems). The nuclei were scanned with an axial distance of 300 nm between consecutive light optical sections, and the confocal image stacks were processed with ImageJ software . Quantitative 3D evaluation was carried out using the voxel-based software algorithms 3D-RRD (Three Dimensional Relative Radius Distribution)  and eADS (enhanced absolute 3D distances to surfaces) Significant differences (P = 0.05) between radial probe distributions were determined using the Mann-Whitney rank sum test (U-test).
Chromatin immunoprecipitation (ChiP) assays
About 6 × 106 cells were treated with 1% formaldehyde and sonicated in 600 μl of protease inhibitor-containing buffer, and then the chromatin was immunoprecipitated with a rabbit anti-serum anti-acetyl histone H4 (Upstate Biotechnology) essentially according to the manufacturer's specifications. The input DNA was an aliquot of the supernatant from each centrifuged sonicate (DNA size range: about 200–600 bp), and the pre-immune chromatin was immunoprecipitated with normal rabbit IgG (Santa Cruz Biotechnology). Pre-clearing before the addition of antibody was carried out for 1 h at 4°C with constant agitation using 10 μl of tRNA (20 mg/ml), 20 μl salmon sperm DNA (10 mg/ml) and 20 μl protein G-agarose beads added to 100 μl sample for each immunoprecipitation. The immunoprecipitates were collected for 3 h at 4°C with constant agitation using 10 μl of tRNA (20 mg/ml), 20 μl salmon sperm DNA (10 mg/ml) and 20 μl protein A-agarose beads added to the 1 ml samples. The purified immunoprecipitated DNA was dissolved in 60 μl of 10 mM Tris-HCl (pH 8.0) 1 mM EDTA. Primer pairs are reported in Additional file 5. The primer pairs to beused in the ChIP assays were derived from the PCR analysis of panels of gorilla, chimpanzee and human somatic cell hybrids (from M. Rocchi and ). For the FRG1 gene, we derived five primer pairs that spanned 1500 bp upstream of the ATG, and produced amplification products only with the DNA from human chromosome 4. Using one such primer pair, the same result was obtained on panels of somatic cell hybrids containing gorilla and chimpanzee chromosomes. On the basis of these results, it can be assumed that this FRG1 primer pair is chromosome 4-specific in each of the analysed species (see example in Additional file 8), and so it was used for the ChIP assay of the FRG1 promoter. The primer pairs for the FRG2 promoter and 13E11 marker (shown in Additional file 5) were selected using a comparable approach. For both PCR reactions, an amplification product was observed only on the ape hybrids retaining chromosome 4, whereas an additional amplification band was detected on chromosome 10 in the human hybrids. However, on the basis of the ChIP analysis of a somatic cell hybrid retaining only chromosome 10, both the FRG2 promoter and 13E11 marker are very poorly histone 4 acetylated, a finding that allowed us to conclude that the high level of histone 4 acetylation observed in the human cells essentially comes from chromosome 4.
The HDAC inhibitor Trichostatin A (TSA, Sigma) was dissolved in EtOH and added to the culture medium at a final concentration of 330 nM for 16 h. An equal volume of EtOH was added to the medium of the untreated control cells.
RNA isolation and real-time RT-PCR analysis
Total RNA was isolated from GGO, PTR and HSA lymphoblastoid cells using the RNA Wiz™ reagent (Ambion) according to the manufacturer's protocol. The integrity of the RNA was confirmed by denaturing gel electrophoresis. Real time RT-PCR analysis was performed on the iQ5 Real-Time PCR Detection System (BIORAD) using the iScriptTM One-Step RT-PCR Kit with SYBR® Green (Biorad). The primers used for amplification are shown in Additional file 5. The specificity of the amplifications was assessed by means of electrophoretic separation of the amplified products and melting curve analysis. The relative expression of the investigated genes was quantified after normalisation with beta 2 microglobulin mRNA (B2M) [45, 46]. All of the RT-PCR analyses were performed in triplicate in three independent experiments. The analyses made using primer pairs on the FRG1 and FRG2 promoters in human, chimpanzee and gorilla somatic cell hybrids strongly indicate that duplicated human and primate FRG1 and FRG2 genes carry divergent or incomplete promoter regions (with the exception of the FRG2 promoter on chromosome 10). However, RT-PCR analysis of a somatic cell hybrid retaining only chromosome 10 showed that the FRG2 gene is very poorly transcribed. These results allowed us to conclude that the transcripts we observed in each species were genuinely from chromosome 4.
facioscapulohumeral muscular dystrophy
long interspersed nucleotide element
- FRG1 and FRG2:
FSHD region genes 1 and 2
- ANT1 :
adenine nucleotide translocator 1 gene
bacterial artificial chromosome
polymerase chain reaction
fluorescent in situ hybridisation
average relative radius
three dimensional relative radius distribution
enhanced absolute 3D distances to surfaces
This study was supported by grants from PRIN 2004 to EG and MR. Financial support was also received from MIUR (Ministero Italiano della Università e della Ricerca; Cluster C03, Prog. L.488/92) and European Commission (INPRIMAT, QLRI-CT-2002-01325) to MR, and Deutsche Forschungsgemeinschaft grant MU 1850/2-1to SM.
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