Differential selection on gene translation efficiency between the filamentous fungus Ashbya gossypii and yeasts
- Huifeng Jiang†1, 2, 3,
- Yue Zhang†2,
- Jun Sun3, 4,
- Wen Wang2Email author and
- Zhenglong Gu1Email author
© Jiang et al; licensee BioMed Central Ltd. 2008
Received: 02 June 2008
Accepted: 29 December 2008
Published: 29 December 2008
The filamentous fungus Ashbya gossypii grows into a multicellular mycelium that is distinct from the unicellular morphology of its closely related yeast species. It has been proposed that genes important for cell cycle regulation play central roles for such phenotypic differences. Because A. gossypii shares an almost identical set of cell cycle genes with the typical yeast Saccharomyces cerevisiae, the differences might occur at the level of orthologous gene regulation. Codon usage patterns were compared to identify orthologous genes with different gene regulation between A. gossypii and nine closely related yeast species.
Here we identified 3,151 orthologous genes between A. gossypii and nine yeast species. Two groups of genes with significant differences in codon usage (gene translation efficiency) were identified between A. gossypii and yeasts. 333 genes (Group I) and 552 genes (Group II) have significantly higher translation efficiency in A. gossypii and yeasts, respectively. Functional enrichment and pathway analysis show that Group I genes are significantly enriched with cell cycle functions whereas Group II genes are biased toward metabolic functions.
Because translation efficiency of a gene is closely related to its functional importance, the observed functional distributions of orthologous genes with different translation efficiency might account for phenotypic differentiation between A. gossypii and yeast species. The results shed light on the mechanisms for pseudohyphal growth in pathogenic yeast species.
Filamentous and yeast-like growths are two ways for fungi to propagate in nature. Under normal growth conditions, filamentous fungi grow as multicellular mycelium but yeasts form unicellular colonies. Most species in the clade Saccharomycotina are yeasts. In some special environments, these species can switch to pseudohyphal growth that is morphologically similar to filamentous growth, but they can not form real hyphal filaments [1, 2]. Ashbya gossypii, however, is classified as a filamentous fungus because it forms natural hyphal filaments for most of its life cycle [3, 4]. What the nature of the molecular differences between A. gossypii and yeasts is that led to such a phenotypic divergence remains an open question. It has been proposed that the divergence might result from a modified cell cycle processes in A. gossypii [4–6]. However, comparative genomics studies reveal that A. gossypii shares an almost identical set of cell cycle genes with the typical yeast Saccharomyces cerevisiae . Although loss or gain of genes among different species often play important roles in phenotypic diversity , divergence of regulation in orthologous genes can also contribute significantly to this process . The fact that A. gossypii and its closely related yeasts share a very similar set of cell cycle genes prompts us to investigate whether these genes have unique regulation patterns in A. gossypii.
Genetic codes exhibit significant usage bias. Selection for gene translation efficiency is the most common Darwinian explanation for codon usage bias. Under this scenario, codons corresponding to abundant tRNA would be preferentially used [9–12]. Natural selection biases such preferred codons so as to enhance accuracy and speed of protein synthesis in highly expressed genes [13–15]. Therefore, differential translation efficiency reflects protein expression difference, which in return can indicate differential functional requirements for these proteins . Therefore, divergence of translation efficiency could reveal orthologous gene functional differentiation across species . By comparing codon usage patterns for orthologous genes between A. gossypii and nine yeast species , we show in this study that in A. gossypii genes with significantly higher translation efficiency are enriched in cell cycle processes, whereas genes with significantly lower translation efficiency are enriched in metabolic processes. Both these findings could account for phenotypic differentiation between multicellular A. gossypii and unicellular yeasts, and would shed light on the evolutionary processes that enable pseudohyphal growth in pathogenic yeast species.
tRNA adaptation index (tAI)
Within a genome, codon usage, which matches tRNA pool of the organism, is an important indicator for gene translational efficiency . In order to detect the differences in gene translational efficiency between A. gossypii and yeasts, a gene matrix containing 3,151 orthologous gene groups for A. gossypii and nine yeast species was constructed. Species-specific tRNA adaptation index (tAI), a measurement that evaluates translation efficiency of coding sequences , was predicted for each gene based on tRNA pool in that species. Because estimation of codon usage can be affected by species-specific factors such as genome-wide GC contents (Additional file 1), in order to have cross species comparisons, we normalized the tAI value of each gene by the median tAI value of all studied orthologous genes in the same species. The tAI values from nine yeast species were used to calculate the likelihood of observing the tAI value for A. gossypii gene in each orthologous gene group.
GO (Gene Ontology) functional enrichment analysis
KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis
KEGG pathways that are enriched in Group I or Group II genes
Total gene number
Gene num. in Group I
Gene num. in Group II
Gene num. in Group III
Cell cycle – yeast a
Basal transcription factors a
Ubiquitin mediated proteolysis a
Lysine biosynthesis b
Biosynthesis of steroids b
Aminoacyl-tRNA biosynthesis b
Comparative analysis with experimentally confirmed genes in A. gossypii
Experimentally confirmed cell cycle genes in A. gossypii
Gene name in A. gossypii
Orthologous ORF name in S. cerevisiae
Gladfelter et al. 2007 
Wendland et al. 2001 
Wendland et al. 2001 
Gladfelter et al. 2007 
Gladfelter et al. 2007 
Wendland et al. 2005 
Hungerbuehler et al. 2007 
Gladfelter et al. 2007 
Wendland 2000a 
Helfer et al. 2006 
Wendland et al. 2005 
Gladfelter et al. 2007 
Bauer et al. 2004 
Gladfelter et al. 2007 
Knechtle et al. 2006 
Schmitz et al. 2005 
Gladfelter et al. 2006 
Walther et al. 2005 
Knechtle et al. 2003 
Wendland 2003 
Wendland 2002 
Codon usage bias in A. gossypii
Among studied species, A. gossypii has the highest genome-wide GC content (0.53), while the GC contents in other species are from 0.35 to 0.46 (Additional file 1A). Correlation coefficients of codon usage between A. gossypii and other nine species show strongly positive relationship with their GC contents, indicating that GC content might intensively shape codon usage in A. gossypii. Relationships of GC contents (estimated by GC3s: GC content at the third site of each codon) and codon usage bias (measured by Nc: effective number of codons which is a parameter measuring overall gene codon bias) on further analysis in all studies species show us that the higher GC3s is, the stronger correlation between GC3s and Nc is (Additional file 1B). For all studied orthologs in A. gossypii, GC3s is strongly correlated with Nc, but not strongly associated with tAI and CAI (codon adaptive index) (Additional file 1C), indicating that change of GC content might have a larger impact on gene Nc than on tAI and CAI values. As in Man and Piple , we obtained low correlations between tAI and Nc, and between CAI and Nc (Additional file 1C), which might be due to the dramatic change of GC content, thus Nc values in A. gossypii. Nevertheless, both correlations are still statistically significant, while tAI and CAI show a very strong positive correlation in this species (Additional file 1C). Because tAI values for orthologs among studied species were normalized to remove the impact caused by some factors at genome level including GC content, it would still be an accurate indicator for expression level, thus the importance of A. gossypii genes during evolution.
Possible effect of protein size on gene translation efficiency
Higher translation efficiency of cell cycle genes in A. gossypii
Comparison of translation efficiencies for orthologous genes should uncover regulation divergence, and thus the differential functional importance of those genes in diverged species [14, 15]. Here by a comparative genomic approach, we identified two groups of orthologous genes showing differential translation efficiency between the filamentous fungus A. gossypii and its closely related yeast species. Genes in Group I have significantly higher translation efficiency in A. gossypii than those in yeasts. Functional analysis indicates that these genes are significantly enriched in cell cycle processes. Subsequent analysis of known pathways and experimentally studied genes in A. gossypii support such conclusion. Because enhanced translation efficiency indicates increased functional requirement for a gene, our results suggest that the roles of cell cycle genes might be substantially different between A. gossypii and yeasts. This suggestion is consistent with the observation that cell cycle processes, such as polar growth modes, cell cycle control and asynchronous nuclear division, are more complex in A. gossypii than those in yeast species [4, 6, 20, 21]. Further experimental investigations for regulatory changes of cell cycle genes in A. gossypii could provide more insights into this conclusion.
For most pathogenic yeasts, transition from yeast form to filamentous form (pseudohyphal growth) is a key mark for their pathogenicity [1, 22]. It has been reported that cell cycle genes play important roles in the morphological transition [1, 23]. Micro-array experiment also shows that cell cycle genes are more up-regulated in S. cerevisiae filamentous form than are those in yeast form . Our results indicate that cell cycle genes possess higher translational efficiency, and thus might be functionally more important, in filamentous fungus than those in yeast. Therefore, understanding species difference between filamentous fungus and yeasts could be helpful for illustrating mechanisms underlying filamentous growth of pathogenic yeasts.
Higher translation efficiency for metabolic genes in yeasts
Genes in Group II have significantly lower translation efficiency in A. gossypii than those in yeasts. Functional enrichment analysis indicates that these genes are enriched for metabolic process, such as amino acid and steroid biosynthesis. This functional distribution might be associated with rapid cell growth in yeasts through fermentative life style. All yeast species studied can carry out fermentative growth under anaerobic or aerobic conditions, but A. gossypii can only conduct aerobic respiration . The cell division rate is usually much faster for fermentative than for respiratory growth . For eukaryotic organisms, cell size is an important control at check points for cell division [26, 27]. In order to attain critical size, cells need to synthesize cellular components much more rapidly during fermentative than during respiratory growth. Therefore the metabolic processes should be in greater demand in yeasts than that in A. gossypii. The difference in metabolic rate could lead to differential translation efficiency for metabolic genes between A. gossypii and yeasts.
Comparative studies revealed that more than 95% genes in multicellular organism A. gossypii share orthologs with unicellular organism S. cerevisiae . It has been proposed that different growth modes between these species were controlled by a very similar gene set [4–6]. Our studies of orthologous genes among A. gossypii and several yeast species show higher translation efficiency of cell cycle genes in A. gossypii, which is consistent with more complicated cell cycle processes in this multicellular organism. On the other hand, our results are also consistent with the faster growth of unicellular yeasts, which leads to greater demands of gene products for metabolic processes. Emergence of multicellular organisms was one of the most profound developmental transitions in the history of life . Our results in this study, and further investigation for the difference between A. gossypii and yeast species might also be important for understanding this process.
Species and sequence data
According to the phylogenetic tree in Fitzpatrick et al. (2006) , 12 genomes have been sequenced in the clade Saccharomycotina. Among these species, only Ashbya gossypii is classified as filamentous fungus. For the remaining 11 yeast species, nine were used in our study (Saccharomyces cerevisiae, Saccharomyces paradoxus, Saccharomyces mikatae, Saccharomyces bayanus, Candida glabrata, Kluyveromyces polysporus, Kluyveromyces waltii, Saccharomyces kluyveri, and Kluyveromyces lactis). Saccharomyces kudriavzevii and Saccharomyces castellii were not used due to their low sequencing coverage. The protein, coding and genomic sequences for recently sequenced K. polysporus are from Scannell et al 2007 . Sequences for other species were downloaded from Fungal Comparative Genomics database  and National Center for Biotechnology Information .
Using InParanoid  program, orthologs between every two species were identified. According to the results from InParanoid, we used MultiParanoid  to generate orthologous matrix among all studied species. Each row in the matrix corresponds to an orthlogous gene shared by all studied species and each column to a species. Duplicated genes detected by InParanoid in one species were classified into the same orthologous group. Only orthologous groups which contain at least one gene for each species were used in our study. As a result, an orthologous matrix with 3,151 rows (genes) and 10 columns (species) was generated.
tRNA adaptation index (tAI)
First, we used tRNAscan-SE software (version 1.1) to predict copy numbers for all tRNA genes (tRNA gene pool) in each genome . The tRNA pools among closely related species are conserved (See additional file 5 for the original data used to perform this analysis) . For species with relatively low genome sequencing quality, we used tRNA pools from their closely related species, a same approach as in Ref 15: the predicted tRNA pool from S. cerevisiae was used to replace the ones in S. byanus, S. paradoxus, S. mikatae and the tRNA pool from K. lactis was used to replace the ones in K. waltii and S. kluyveri.
Second, given coding sequence for a gene, we used a modified tAI.R program  to estimate its tAI value in each species according to the tRNA pools (we kept all codons for methionines expect the start codons). When an orthologous group contains more than one gene in a species, the average tAI value of these genes was used for that species.
Finally, we normalized tAI value of each gene by median tAI value for all studied genes in the same species. For each orthologous group, we used the tAI values for nine yeast species to construct a normal distribution model, which was then used to estimate the probability of observing tAI value for the A. gossypii gene. Under 95% confidence level, we identified 333 (Group I) and 552 genes (Group II) for which orthologs from A. gossypii have significantly higher and lower tAI values than those from yeasts, respectively. The rest 2,266 genes without significantly different tAI values between A. gossypii and yeasts were classified in Group III.
Functional enrichment analysis
GO (Gene Ontology) annotations of S. cerevisiae genes were used for our analysis. We identified functional enrichment of genes in each group (group I, II and III) by GO term finder . The functional enrichments were determined at P-value < 0.05. Pathway information for S. cerevisiae was downloaded from KEGG database http://www.genome.jp/kegg/. All studied orthologous genes in S. cerevisiae were mapped onto these pathways. For each pathway, a chi-square test was used to calculate the probability of gene number distribution among each group.
We thank Chuan Gao, Orna Man for technical assistance, Lin Xu for discussion and David Pinney for reading the manuscript. This work was supported by a NSFC key grant (No. 30430400) and a 973 Program of China (No. 2007CB815703-5) to WW and startup funds from Cornell University awarded to ZG.
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