Shape, colour plasticity, and habitat use indicate morph-specific camouflage strategies in a marine shrimp
© The Author(s). 2016
Received: 13 August 2016
Accepted: 10 October 2016
Published: 18 October 2016
Colour and shape polymorphisms are important features of many species and may allow individuals to exploit a wider array of habitats, including through behavioural differences among morphs. In addition, differences among individuals in behaviour and morphology may reflect different strategies, for example utilising different approaches to camouflage. Hippolyte obliquimanus is a small shrimp species inhabiting different shallow-water vegetated habitats. Populations comprise two main morphs: homogeneous shrimp of variable colour (H) and transparent individuals with coloured stripes (ST). These morphs follow different distribution patterns between their main algal habitats; the brown weed Sargassum furcatum and the pink-red weed Galaxaura marginata. In this study, we first investigated morph-specific colour change and habitat selection, as mechanisms underlying camouflage and spatial distribution patterns in nature. Then, we examined habitat fidelity, mobility, and morphological traits, further indicating patterns of habitat use.
H shrimp are capable of changing colour in just a few days towards their algal background, achieving better concealment in the more marginal, and less preferred, red weed habitat. Furthermore, laboratory trials showed that habitat fidelity is higher for H shrimp, whereas swimming activity is higher for the ST morph, aligned to morphological evidence indicating these two morphs comprise a more benthic (H) and a more pelagic (ST) life-style, respectively.
Results suggest that H shrimp utilise a camouflage strategy specialised to a limited number of backgrounds at any one time, whereas ST individuals comprise a phenotype with more generalist camouflage (transparency) linked to a more generalist background utilisation. The coexistence within a population of distinct morphotypes with apparently alternative strategies of habitat use and camouflage may reflect differential responses to substantial seasonal changes in macroalgal cover. Our findings also demonstrate how colour change, behaviour, morphology, and background use all interact in achieving camouflage.
KeywordsCamouflage strategy Caridean shrimp Polymorphism Geometric morphometrics Colour change Habitat use Life-styles
Polymorphism is a common trait in many animal taxa [1, 2] and has been a subject of numerous empirical studies testing several evolutionary theories and hypotheses (e.g. [3–5]). Aside from facilitating the exploitation of a wider array of habitats [6–9], polymorphism may also involve a segregation of behavioural traits among morphs, such as related to differences in mating tactics [10, 11] or habitat use [12, 13]. Morph-specific morphological and behavioural traits can allow individuals to more efficiently gather resources and exploit different niches through the diversification and specialisation of life-history strategies [6, 14, 15]. Identifying the selective forces responsible for the origins and maintenance of morphs, and unravelling their relative advantages, are important tasks in order to predict population dynamics in varying environments and for understanding evolutionary and developmental strategies [2, 16, 17].
One of the most longstanding areas where colour and shape polymorphisms have been studied in nature relates to camouflage [4, 8, 18–20]. Habitat-specific camouflage of colour morphs may be obtained via a number of mechanisms, whereby behavioural and morphological traits of individuals can interact with environmental characteristics to reduce their relative risk of predation [21–23]. For instance, individual appearance for camouflage can be either attained through genetic polymorphism [24, 25], or through colour change and phenotypic plasticity [26–28]. In addition to changes in appearance, camouflage can also be driven by the behavioural preferences of individuals to rest on backgrounds that provide enhanced camouflage [23, 29, 30]. Evidence of morph-specific behavioural preferences for substrate types has been observed in a variety of taxa, including moths , grasshoppers  and crabs , and we would expect this to be common if morphs have evolved under selection for camouflage against different substrates. Therefore, camouflage in polymorphic species should be driven by both colour change in line with the predominant visual background, and behavioural preferences for individuals to rest on backgrounds that they match.
The degree to which different morphs can exploit alternative (micro) habitats should depend on how effectively individuals can conceal themselves against the background. Therefore, for species living in heterogeneous substrates, different morphs may be effectively concealed in microhabitats with different background colour patterns within the same general environment [17, 33, 34]. In this case, predation by visual consumers may drive disruptive selection leading to individuals specializing in each of the available backgrounds [35, 36], and/or the ability of some individuals to change colour depending on the patch type they live on [26, 37, 38]. On the other hand, a more generalist fixed strategy may be favoured when optimal colouration is achieved by a compromise in the degree of crypsis obtained in different microhabitats while matching no background very closely [33, 39], or through camouflage types that are less restricted to one background type alone (e.g. transparency).
Differential coloration and camouflage strategies may evolve together with both morphological and behavioural traits in polymorphic species [21, 32]. For example, colour patterns in Midas cichlid fish are correlated to both body shape and life-style, with golden deeper bodied fish mostly associated to the benthic habitat, and dark slender individuals exhibiting a more limnetic life-style . Also, Dalmatian wall lizards comprise three different colour morphs, with different body and relative head size, which relate to morph-specific trophic niches and cross-habitat distributions . Theory also predicts that morphs with a specialist camouflage strategy would concentrate in habitat patches where concealment is most efficient, increasing substrate fidelity and lowering predation risk [32, 41]. Active preference for these patches may lead to exceptionally high population densities, only constrained by habitat carrying capacity , favouring high intra-specific competition, with some individuals being displaced to marginal habitat patches . Alternatively, for individuals with a generalist strategy, in which camouflage is less constrained to a limited number of backgrounds, selection may favour a more opportunistic life-style with individuals possessing differential morphology and behaviour [17, 44]. A generalist life-style with lower habitat fidelity and increased mobility may allow individuals to reduce competitive interactions and facilitate more efficient resource exploitation and mate searching [45, 46]. Strong specialization, coupled to habitat fidelity, and high mobility associated to a more opportunistic use of resources, can be found in different morphs within populations, and their coexistence is apparently mediated by environmental conditions dictating relative fitness of individuals at different frequencies [47, 48].
The natural distribution of H. obliquimanus individuals between algal habitats is clearly morph-specific . H individuals tend to occupy colour-matching substrates, i.e. greenish-brown shrimp are more abundant in Sargassum, while pink individuals in Galaxaura, and ST shrimp are equally distributed between these macroalgae . While over a period of days and weeks H shrimp may be able to change colour to different substrate types (see below), at any one time they should be restricted to one matching background type alone, and hence we consider them background specialists (but note that over time they may be considered generalists). Mismatching shrimp, i.e. HGB in Galaxaura or HP in Sargassum, are very probably individuals that arrived from a different habitat and had not yet adjusted to local background. In contrast, ST individuals may adopt more generalist background choice behaviour and a camouflage type (transparency) that allows concealment to a range of substrate types. Sex proportions are also different between morphs, with H shrimp being chiefly females and ST mostly males, suggesting that selection for sex-specific traits may be also important in explaining the maintenance of polymorphism in this species . Morph-specific habitat and sex distribution may indicate the existence of behavioural differences between morphotypes [32, 51], possibly related to contrasting strategies of habitat use. In the case of H. obliquimanus, cryptic behaviour is expected to be selected in H shrimp, with individuals remaining on colour-matching backgrounds, and a more general life-style is anticipated for transparent ST individuals, which would move more frequently among different substrate types.
In this study, we used a combination of laboratory manipulative experiments, supported by geometric morphometric analyses, to test the hypothesis that colour morphs of H. obliquimanus differ in specific behavioural traits and morphology related to strategies of camouflage and habitat use (namely Sargassum and Galaxaura canopy). We first examined two potential mechanisms by which individuals can enhance crypsis: habitat selection and colour change. We undertook experiments of behavioural habitat selection to test whether morphs actively select the background-matching macroalgal habitat where concealment is more effective. Then, we performed a colour change experiment to investigate if the capacity of colour change differs between morphs and habitats. Because carapace shape can be a proxy for life-style and habitat use in caridean shrimps, with stout forms being an indicative of benthic life-style and more streamlined shapes of a more pelagic behaviour [52, 53], we used geometric morphometric analyses and carried out experiments of habitat use to verify whether morphological evidence correlates with behavioural patterns. Together, the results of this study evidenced a link among coloration, morphological, and behavioural traits, illustrating how polymorphism can be advantageous to individuals achieve different camouflage strategies when living in a heterogeneous habitat.
Samples of the macroalgae Sargassum and Galaxaura were collected during the summer and autumn of 2011, 2013 and 2015 by skin diving at rocky bottoms in different sites along the São Sebastião Channel (23°49′38″S; 45°25′16″W; São Sebastião, SP, Brazil). Individuals of H. obliquimanus were sorted out from the macroalgae (as in ), visually classified as HGB, HP or ST, and used in laboratory experiments to compare morph-specific algal preferences, colour change capacities and behaviour. We validated this visual classification by running a discriminant function analysis (DFA), using the ‘lda’ function from the package MASS in R , on random samples of individuals initially classified as HGB and HP (n = 10), to which colour reflectance values in image RGB colour channels were measured (as described below in ‘Colour change and camouflage’). DFA scores for these morphs were discrete and non-overlapping (DFA scores: −5.54 < HGB < −2.46; 2.13 < HP < 5.12) indicating that misclassifications were very unlikely.
Individuals were first acclimated to laboratory conditions for three days and kept in indoor tanks, with their original plant hosts, at ambient temperature and with filtered running seawater and artificial aeration. At the start of the experiments, shrimp were transferred to rectangular plastic aquaria (30 × 20 × 10 cm) and maintained at nearly constant temperature (25 °C). In all experiments, the position of aquaria assigned to different experimental treatments was randomly chosen to avoid potential artefacts due to uncontrolled spatial variation of any physical variables within the laboratory room.
General procedures followed standard protocols for multiple-choice tests (e.g. [55, 56]). Algae were supplied in equivalent quantities (20 ml) as single clumps anchored to opposite corners of the aquaria (n = 12 for each morph). Fifteen individuals (HGB, HP, or ST) were added to the centre of each aquarium and, after 3 days, algae were carefully enclosed in dip nets and the number of living shrimp counted. As a response variable, we used the difference between the shrimp found at Sargassum and Galaxaura, divided by the total number of shrimp remaining alive at the end of the experiment, to account for mortality (2.8 shrimps ± 0.3). These preference indices were compared among morphs using a 1-way ANOVA. The Student-Newman-Keuls (SNK) procedure was used for a posteriori comparisons. Confidence intervals (95 %) were additionally calculated for each morph.
Colour change and camouflage
Previous observations indicated that the capacity of colour change differs between shrimp morphs, with H individuals visually changing their body colour in few days when exposed to an unmatched algal habitat, and ST shrimp being unable to change their coloration in the same period . In this study we restricted further and more detailed analyses of colour change to the H morph. We cannot discard long-term colour shifts in ST shrimp, but because transparent individuals are typically characterized by a much reduced number of colour cells and pigments along the body, as observed in the closely related species Hippolyte varians  and Heptacarpus pictus , their eventual reorganization would likely respond to a different physiological process , acting over longer time-scales (weeks or months [27, 28]).
Here, we conducted an experiment to quantify colour change and camouflage in the plastic morph (H), exposing individuals of varying coloration (greenish-brown and pink) to different algal habitats and artificial substrates. By doing this, we aimed to (i) test whether short-term colour changes are possible on these substrates, (ii) examine if the mechanisms controlling colour change in this species depend on visual information or diet by keeping individuals on either artificial or natural substrates, with food resources only available in the latter, and (iii) compare the efficiency of colour alteration to provide camouflage in morphs exposed to colour matched and unmatched backgrounds. Although we acknowledge that it would have been ideal to do so, colour metrics were not quantified before the trials because handling of these small and fragile shrimp could likely alter their behaviour and cause excessive mortality. We therefore used the final colour of shrimp kept against a matching background as their standard in nature. This assumption was tested by comparing hue values (see below) between experimental shrimp on matching backgrounds with shrimp freshly collected in the field (n = 10 for each morph); i.e. experimental HGB on Sargassum vs. natural HGB, and experimental HP on Galaxaura vs. natural HP.
We measured colour for individual algae and shrimp in all experimental treatments using digital image analyses, which provides a powerful and non-invasive approach to quantify animal coloration . A Nikon Coolpix P5000 camera, coupled to a stereomicroscope and a constant white light source of 3200 K colour temperature, was used to obtain all images. Samples were photographed using manual white balancing and exposure settings to avoid colour saturation , followed by photographs of one standard grey card (Color Checker Passport, X-Rite), reflecting light equally at 35 % between 400 and 750 nm, using the same camera settings, as required by the sequential method of calibration . Before obtaining colour data, each image was linearised to control for changes in light intensity using a set of six grey references from the colour checker chart (Color Checker Passport, X-Rite), based on the methods described by Westland and Ripamonti  and Stevens et al. . This procedure was necessary because many digital cameras show non-linear responses of image values to changes in light levels that need to be corrected before obtaining accurate data. The camera responses were also equalised in relation to the 35 % standard grey card to control for changes in the illuminating light conditions. Finally, images were scaled to reflectance values in red (longwave; LW), green (mediumwave; MW), and blue (shortwave; SW) layers (an image value of 255 on an 8-bit scale is equal to 100 % reflectance ).
For each shrimp or algal image, we measured regions of interest (ROIs) and sampled the values of reflectance in the red, green, and blue channels (RGB) using the program ImageJ . For shrimp images, we selected one square (1.5 mm2) on the abdominal region of individuals, between somites 2 and 3, where colour is particularly uniform, and for algal images we selected the entire frond outline (approx. 50 mm2). For shrimp data, we obtained values of colour (hue), which was calculated as the red/green ratio, broadly analogous to the general principle of an opponent colour channels, whereby colour types are encoded by antagonistic neural pathways [63, 64] and similar to other past studies [37, 65]. Red, grey, and green tones would provide hue values > 1.0, ≈ 1.0 and < 1.0, respectively. The use of this metric does not depend of any specific visual system or predator group , allowing us to analyse colour in terms of the physical properties of each shrimp in an intuitive way.
We prepared two replicate aquaria for each treatment combination of ‘morph’ (HGB, HP) and ‘background colour’ (brown, pink). Parallel trials were run using 20 ml substrates of either natural (brown Sargassum and pink Galaxaura) or artificial background (assembled stripes of brown and pink plastic tape), summing up 16 experimental units. Artificial substrates matched algal tones as closely as possible, while providing intermediate habitat architecture between the highly intricate Sargassum matrix and the smoother Galaxaura habitat. Seven to eight shrimp were initially added to each of these aquaria, with individuals maintained in artificial substrates supplied pellet shrimp food daily. Air pumps ensured adequate water circulation and aeration. In all treatments, individuals were recovered after 5 days, immediately frozen (a procedure that did not alter their colour), and later photographed to obtain colour values. A few shrimp were lost (possibly owing to mortality) and we had to reduce sample size to the minimum number of individuals found across aquaria (n = 5, for both parallel trials using natural and artificial substrates), ensuring a balanced design. Excess individuals from remaining aquaria were randomly excluded from analyses. To test the ability of individuals to change colour, we compared hue values separately for each experiment (natural or artificial substrates) using a mixed three-factor ANOVA in which factors ‘morph’ (HGB or HP) and ‘substrate colour’ (brown or pink) were fixed and orthogonal, and the factor ‘aquaria’, with two levels, was random and nested in the interaction between main factors. The Student-Newman-Keuls (SNK) procedure was used for a posteriori comparisons.
We also aimed to quantify the efficiency of colour change to provide camouflage against both algae. For that, we compared the final colour of shrimp reared in the different experimental treatments to the actual colour of both Sargassum and Galaxaura. We first standardised the reflectance data in the three colour channels (RGB) of shrimp and algae and then converted these values to x and y coordinates in a trichromatic colour space . Colour departures were calculated as the Euclidian distances between coordinates of replicate shrimp and algae. Replicate algal coordinates (n = 20) were randomly split in two groups, to provide independent and balanced distance estimates between algae and shrimp for each morph. We used t-tests, corrected for heteroscedasticity when needed, to compare colour coordinates of each shrimp morph against the colour of both algae, predicting that shrimp colour would be closer to the colour of their rearing background than to the colour of the alternative algal background.
Morphological and behavioural differences between morphs
Intraspecific plasticity of body shape, which substantially affects hydrodynamics, is commonplace in a variety of aquatic invertebrates and fish, and may indicate differential patterns of habitat use and behaviour [15, 52, 53, 67]. Because H and ST morphs were differently distributed between algal habitats and possibly subjected to distinct selective forces , we predict that H. obliquimanus individuals will exhibit morph-specific shape, with possible consequences on shrimp behaviour and life-style. Since homogeneous individuals can change their colour in just a few days (see Results), we pooled the HGB and HP categories together in a single group (H) for follow-up comparisons on morphology and behaviour.
We used geometric morphometric analyses to compare carapace shape differences between morphs. Analyses were restricted to males to eliminate any variability owing to sexual dimorphism. Twenty-one H and 25 ST individuals were sorted from samples of Sargassum and Galaxaura collected in the São Sebastião Channel (as in ). Shrimp were fixed in 70 % ethanol, stained with rose bengal, and their left carapace side was photographed using a Nikon Coolpix P5000 camera, coupled to a stereomicroscope set at a magnification power of 10×.
Nine landmarks were defined along the margin of the carapace as follows; 1: eye orbit, 2: rostral tip, 3: first dorsal spine, 4: mid-dorsal margin, vertically opposed to landmark 8, 5: posterior dorsal edge, 6: posterior lateral tip, 7: distal ventral margin, vertically opposed to landmark 5, 8: ventral-most point, opposite to landmark 4, 9: ventral insertion point of the antennule. Landmarks were defined using the software tpsDig 2.14 , following standardized criteria . Landmark alignment and the acquisition of shape variables, both uniform components (UCs) and relative-warps (RWs), were carried out following the procedures described by Zelditch and co-workers , using the software tpsRelw 1.46 .
The values of UCs and RWs were separately compared between H and ST individuals, using multivariate analysis of variance (MANOVA). Centroid size (CS), i.e. the square root of the summed squared distances between all landmarks and the carapace centre of gravity (centroid), was used as a size variable and compared between colour morphs with a t-test.
Habitat fidelity and mobility
We compared substrate fidelity and individual mobility between morphs in a simple laboratory experiment. Trials were performed in plastic rectangular aquaria (30 × 20 × 10 cm) provided with a longitudinal flow of 2 l/min containing a single Sargassum clump (40 ml) attached to the upstream end, and 20 shrimps, 10 HGB and 10 ST, at the opposite downstream side. We used only Sargassum as habitat in this experiment because this is the algal type supporting the highest shrimp density in the study area , and also because this is the preferred habitat of these colour morphs (see Results). The same experimental setup was replicated five times and, in each trial, all individuals were morph identified (Additional file 1: Figure S1) and monitored using a video camera (Sony HDR-XR250) for 30 min. Five three-minute video samples were selected for analyses, starting at time 1.5 min and taken at every other 3 min intervals, thus providing samples centred at times 3, 9, 15, 21 and 27 min. For each video sample we separated 90 frames (one every 2 s) for analyses. Habitat fidelity was estimated as the percentage of shrimp on algae at frame 45 (at the mid of each sample). In order to quantify mobility, we tracked the position of each shrimp remaining out of algae through all the 90 frames for each period and calculated total travelled distances. These analyses were undertaken using the software ImageJ.
The proportion of shrimp settled on algae was used as a proxy of shrimp habitat fidelity. Between-morph comparisons of these proportions, at different times, were examined using repeated-measures ANOVA because data from the same aquaria are dependent on time. Raw data were used since the sphericity assumption was met (W = 0.089; p = 0.078). Mobility of individuals was first estimated by comparing individual travelled distances between H and ST shrimp using Mann-Whitney tests. Comparisons on ranks did not detect differences between morphs (69.5 < U < 396.5, p > 0.05 for all sampled periods) because most individuals (72 %) moved very little around their initial positions, typically less than 2 cm. Therefore, we proceeded by comparing mobility of the fewer remaining shrimp that did swim considerable distances. Since these were outliers within the whole population (based on an outlier coefficient, k, of 2.0), we first subtracted swimming distances by baseline movement at their respective sampling period, i.e. the upper fence for non-outlying data. These corrected swimming distances were considered independent records and compared between morphs using a t-test.
Colour change and camouflage
Summary results of the mixed three-way analyses of variance testing the effects of morph type (M; greenish-brown or pink), substrate colour (SC; brown or pink) and aquaria (nested in the interaction between main factors) in final hue values measured in Hippolyte obliquimanus individuals after being maintained for five days in artificial or algal substrates
Source of variation
Substrate colour (SC)
M x SC
Aquaria (M x SC)
C = 0.247; ns
C = 0.240; ns
Colour change was very clear in natural algal substrates but not in artificial ones. HGB individuals increased their hue values after being in contact with the red alga Galaxaura, attaining a reddish coloration, and HP shrimp showed the opposite pattern when placed in Sargassum, achieving at the end of the experiment a brownish tone (Fig. 3a). As a result, hue differences between shrimp morphs, within each algal habitat, disappeared at the end of the trial (Fig. 3a; SNK tests, p > 0.05). However, shrimp reared in artificial substrates retained morph-specific hue (thus the significance of ‘morph’, Table 1), with no changes toward background colour (Table 1; Fig. 3b). Hue differences between morphs persisted both in brown (SNK test, p < 0.05) and pink (SNK test, p < 0.01) artificial substrates (Table 1).
Morph-specific morphological and behavioural patterns
Habitat fidelity and mobility
Substrate fidelity was markedly different between H and ST shrimp over time (repeated-measures ANOVA: F (4,32) = 2.77, p = 0.044; Fig. 5b). At the beginning of the experiment (3 min), the proportion of individuals found on algal clumps was low, but virtually the same for each morph. The number of shrimp using the algal habitat tended to increase through time, but the rate at which they stopped swimming and settled on algae differed between H and ST shrimp. At 9 min, differences were already noticeable, increasing thereafter to statistical significance. At the end of the experiment (27 min), 78 % of H shrimp but only 57 % of ST individuals had settled on algae (Fig. 5b).
Mobility above baseline activity was restricted for a small fraction of the population and decreased from 12 % to 6 % over the experiment (Fig. 5c). Most of these swimming individuals were ST shrimp (61 %). Considering all sampled periods, average mobility was higher in ST (15.2 cm.shrimp−1.min−1) than in H shrimp (5.20 cm.shrimp−1.min−1; two-sample t-test: t (17) = 2.20, p = 0.043). It is also important to note that swimming events over distances larger than 25 cm each minute (n = 5) were only recorded for ST shrimp (Fig. 5c).
We report contrasting behavioural and morphological patterns in colour morphs of the shrimp Hippolyte obliquimanus, suggesting a diversification of life-styles between morphs which can be linked to alternative camouflage strategies. Our results indicate that H shrimp are capable of fast colour change, with different colour types concealed in distinct macroalgal habitats. Individuals of this morph are also tightly connected to their benthic habitat, avoiding long-distance swimming away from their host algae, which explains why they concentrate in exceptionally high densities in the brown weed Sargassum . All these features suggest that this morph presents a specialist camouflage strategy, achieved by concealment to a specific background type (at any given point in time, although individuals can change colour over time). In contrast, ST shrimp cannot rapidly adjust their colour to their background environment, and also show low habitat fidelity and substantial swimming activity, indicating a more pelagic life-style. These characteristics are in accordance to their uniform distribution between Sargassum and Galaxaura, the two main vegetated habitats in the study region , suggesting a generalist habitat use linked to a camouflage strategy achieved by transparency. It is noteworthy that the results of experiments on behavioural patterns are consistent with morphological analyses, indicating a more benthic life-style for H shrimp and a more pelagic habit for ST shrimp, encompassing an important range of the morphological variation found in caridean shrimp [52, 53].
Colour change in H shrimp was observed upon contact with living algal habitats, but not artificial substrates, indicating the process of colour change in this species, and possibly in many other algal-dwelling isopods , decapods [7, 57] and fish , relies, at least in part, on substrate-individual interactions. In fact, some authors have shown that the ingestion of carotenoid pigments can promote colour change in other crustaceans [73, 74], typically over a longer period (weeks) than observed in this study. Note that this does not discount a role of visual feedback, and future work should independently change diet and visual appearance to tease apart these effects.
Colour change may be a faster process for small crustaceans shedding thin translucent exuviae (own observations) than large ones, because pigment reorganization in hypodermic colour cells may be readily visible, as observed for another hippolytid shrimp species . Colour change in H shrimp strongly suggests a camouflage strategy by background matching, whereby individuals’ overall body colour, colour pattern, and brightness tend to resemble the general background . However, we observed H shrimp concealed better on the pink Galaxaura than on the brown Sargassum. In Sargassum, HGB and HP ended up with an intermediate body colour pattern, equally distant from the two algal types. In contrast, both shrimp morphs reared in Galaxaura became much better concealed to this substrate than to the alternative Sargassum background. These results were surprising since natural shrimp densities in the brown Sargassum are far higher than in Galaxaura ; a difference that could be explained by more efficient camouflage in the former. Our results, however, indicate that this is not the case, and that factors other than colour camouflage alone likely underlie this species distribution in the field. Also, these findings are aligned to ongoing research suggesting better protection against predators in Galaxaura (in prep), highlighting the importance of concealment in the pink weed habitat. Further work on longer term changes in colour than those tested here are also needed.
The Sargassum and Galaxaura canopy constitute the most important habitat types to shrimp in our study area, but the relative value of these habitats for H. obliquimanus is apparently very different . Experiments in the laboratory testing algal preferences showed that HGB and ST individuals actively select Sargassum fronds while HP shrimp did not show any significant preference, indicating that colour camouflage is not an important selective force setting patterns of habitat choices. Strong preference of individuals for Sargassum may be adaptive for several different reasons not addressed in this study. For instance, as a much more physically complex habitat, especially when associated to epiphytic algae (e.g. Hypnea spp. [76, 77]), Sargassum would probably supply better shelter from predators and more extensive foraging grounds [78, 79] compared to Galaxaura. It is also possible that inconspicuous behaviour coupled to shape resemblance to background details [18, 80, 81] in the more complex Sargassum would ultimately render superior predator avoidance. More specific research addressing these issues is pending.
Habitat fidelity and mobility further support morph-specific life-styles. Colour-changing shrimp (H) show higher substrate fidelity and lower mobility rates indicating a more specialized habitat use. Although capable of colour alteration towards background matching, moving from one algal habitat to another would likely come at a cost. Settling on non-matching habitat for even a few days, compatible to the time for colour adjustment, may lead to very high predation rates [82–84]. Colour change may also carry physiological costs, although these have rarely if ever been quantified . Therefore, at any one time, H morphs may be able to conceal to a specific background type, being considered background specialists. Conversely, ST shrimp may be generally concealed against a wider range of visual backgrounds  while moving from one habitat patch to another. Therefore, the transparency of individuals, linked to a higher mobility and lack of substrate fidelity, may eventually promote camouflage by means of a strategy independent (or partially dependent) of background matching, indicating a more generalist type of concealment and habitat use [33, 35, 39]. Morph-specific life-styles are supported by natural shrimp distributions  and also by geometric morphometrics analyses of carapace shape. The morphological gradient observed overlaps a great deal of the variation for caridean shrimp in general [52, 53]. While the more hydrodynamic shape found in ST shrimp clearly resembles the shape of pelagic shrimp species, the stouter H morphology are more akin to benthic species. More streamlined ST shrimp swimming distances within the range of 25 to 45 cm each minute may easily move across different algal habitats, which is not the case of more sedentary and deep-bodied H shrimp that were never observed swimming over such distances and tended to settle and remain on algae more frequently. Shrimp morphology, perhaps coupled to behaviour, may also affect camouflage in their algal habitats. Further experimental work is required, however, to examine this issue more closely.
While the different colour types of H and ST individuals may reflect distinct life-styles, we might ask what drives selection for these different approaches. Low dispersal and optimization of resource use can be particularly advantageous in H individuals, which concentrate in habitat patches where shelter is abundant and/or camouflage efficient. Even being a habitat where colour camouflage does not appear to be critical, Sargassum supports high densities of H shrimp, which exhibit high preference and fidelity to this habitat. The less structured Galaxaura substrate would be important as a secondary habitat to this morph, where colour concealment will be a valuable mechanism to reduce prey detection by visual predators. Based on these assumptions, we may expect strong intraspecific competition in Sargassum habitat, and hence selection for optimal resource use and territorial behaviour, which would possibly lead to displacement of ST individuals to Galaxaura. Density-dependent processes and loss of preferred habitats could be major mechanisms regulating abundance of H individuals. On the other hand, high dispersal potential and a generalist habitat use may be useful traits for ST shrimp. Because ST shrimp are mainly males , intense mobility and low substrate fidelity would likely enable males to find more mates in a pure-search strategy, expected for polyginic caridean species such as H. obliquimanus [45, 86, 87].
The coexistence within a population of distinct morphs with alternative strategies of habitat use and camouflage, as observed for H. obliquimanus, facilitates diversification on the use of environmental resources  and can also have ecological and evolutionary consequences, mainly on population stability over time . The availability of the presumably higher-quality Sargassum habitat in our study region is markedly seasonal, with very high cover during summer and a much reduced density in winter, sometimes collapsing in that season . Temporal variation in Sargassum cover can be a major mechanism controlling H shrimp densities, once individuals show strong specialization for this habitat. Therefore, the existence of an alternative habitat (Galaxaura) and morphs differing in their degree of habitat specialization may allow temporal changes in individual fitness associated with habitat availability and morphs density and frequency. Ongoing research on trophic niche space would further elucidate morph-specific patterns of resource use.
Colour camouflage is a common anti-predator strategy in nature, but few studies investigate complex interactions among colour traits and other morphological and behavioural mechanisms, indicative of general morph-specific life-styles. Our findings illustrate that specific arrangements among morphology, behaviour, and (micro-) habitat use in colour morphs of the algal-dwelling shrimp H. obliquimanus may result in a diversification of camouflage strategies in a species living in a heterogeneous habitat. Colour change ability and high substrate fidelity, associated to a more robust morphology, suggest a specialist camouflage strategy in H individuals. On the other hand, high mobility coupled with a more streamlined morphology and lack of substrate fidelity in ST individuals, indicate a general strategy of camouflage in this morph. Higher mobility of the ST morph, in which more than 70 % of individuals are males , may also sustain a pure-search polygynic mating strategy which is predicted for this species. Seasonal changes on macroalgal cover may affect the frequency and fitness of the different colour morphs in the population. Selective mechanisms, such as morph-specific predation by visual consumers through contrasting patterns of habitat use [51, 89], would be important forces maintaining the diversification of life-styles and camouflage strategies in this shrimp species.
We are grateful to Alvaro Migotto for his advice in image acquisition, and two anonymous referees for comments on the paper. We especially thank the technician staff at the Center for Marine Biology for helping in field surveys, and Glauco Machado and Fosca Leite for suggestions on an early manuscript draft.
This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (2009/06675-4 and 2012/17003-0), which granted a master and a PhD fellowship to RCD and a visiting professor grant to MS (#2015/22258-5). This is a contribution of the Research Centre for Marine Biodiversity of the University of São Paulo (NP‐Biomar / USP).
Availability of data and materials
The datasets supporting the conclusions of this article are included as an additional file (Additional file 2).
RCD and AAVF participated in the design of the study and in the collection of specimens. RCD conducted the experiments, analysed the data and drafted the manuscript with AAVF and MS. All authors wrote and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
All experimental procedures used in this study comply with the current laws of Brazilian legislation.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Gray SM, McKinnon JS. Linking color polymorphism maintenance and speciation. Trends Ecol Evol. 2007;22:71–9.View ArticlePubMedGoogle Scholar
- Mclean CA, Stuart-Fox D. Geographic variation in animal colour polymorphisms and its role in speciation. Biol Rev. 2014;89:860–73.View ArticlePubMedGoogle Scholar
- Cain AJ, Sheppard PM. Natural selection in Cepaea. Genetics. 1954;39:89–116.PubMedPubMed CentralGoogle Scholar
- Kettlewell H. Selection experiments on industrial melanism in the Lepidoptera. Heredity (Edinb). 1955;9:323–42.View ArticleGoogle Scholar
- Forsman A, Ahnesjö J, Caesar S, Karlsson M. A model of ecological and evolutionary consequences of color polymorphism. Ecology. 2008;89:34–40.View ArticlePubMedGoogle Scholar
- Van Valen L. Morphological variation and width of ecological niche. Am Nat. 1965;99:377–90.View ArticleGoogle Scholar
- Hultgren KM, Stachowicz JJ. Size-related habitat shifts facilitated by positive preference induction in a marine kelp crab. Behav Ecol. 2010;21:329–36.View ArticleGoogle Scholar
- Stevens M, Lown AE, Wood LE. Camouflage and individual variation in shore crabs (Carcinus maenas) from different habitats. PLoS One. 2014;9:1–31.Google Scholar
- Duarte RC, Flores AAV. Morph-specific habitat and sex distribution in the caridean shrimp Hippolyte obliquimanus. J Mar Biol Assoc. United Kingdom [Internet]. 1–8. Available from: http://www.journals.cambridge.org/abstract_S0025315416000230.
- Sinervo B, Lively CM. The rock-paper-scissors game and the evolution of alternative male strategies. Nature. 1996;380:240–3.View ArticleGoogle Scholar
- Martin E, Taborsky M. Alternative male mating tactics in a cichlid, Pelvicachromis pulcher : a comparison of reproductive effort and success. Behav Ecol Sociobiol. 1997;41:311–9.View ArticleGoogle Scholar
- Bourke P, Magnan P, Rodriguez MA. Individual variations in habitat use and morphology in brook charr. J Fish Biol. 1997;51:783–94.View ArticleGoogle Scholar
- Joron M. Polymorphic mimicry, microhabitat use, and sex-specific behaviour. J Evol Biol. 2005;18:547–56.View ArticlePubMedGoogle Scholar
- Bolnick DI, Svanbäck R, Fordyce JA, Yang LH, Davis JM, Hulsey CD, et al. The ecology of individuals: incidence and implications of individual specialization. Am Nat. 2003;161:1–28.View ArticlePubMedGoogle Scholar
- Kusche H, Elmer KR, Meyer A. Sympatric ecological divergence associated with a color polymorphism. BMC Biol. 2015;13:82.View ArticlePubMedPubMed CentralGoogle Scholar
- Bond AB. The evolution of color polymorphism: crypticity, searching images, and apostatic selection. Annu Rev Ecol Evol Syst. 2007;38:489–514.View ArticleGoogle Scholar
- Magellan K, Swartz ER. Crypsis in a heterogeneous environment: relationships between changeable polymorphic colour patterns and behaviour in a galaxiid fish. Freshw Biol. 2013;58:793–9.View ArticleGoogle Scholar
- Hacker S, Madin L. Why habitat architecture and color are important to shrimps living in pelagic Sargassum: use of camouflage and plant-part mimicry. Mar Ecol Prog Ser. 1991;70:143–55.View ArticleGoogle Scholar
- Palma AT, Steneck RS. Does variable coloration in juvenile marine crabs reduce risk of visual predation? Ecology. 2001;82:2961–7.View ArticleGoogle Scholar
- Todd P, Briers R, Ladle R, Middleton F. Phenotype-environment matching in the shore crab (Carcinus maenas). Mar Biol. 2006;148:1357–67.View ArticleGoogle Scholar
- Forsman A, Appelqvist S. Visual predators impose correlational selection on prey color pattern and behavior. Behav Ecol. 1998;9:409–13.View ArticleGoogle Scholar
- Karpestam E, Merilaita S, Forsman A. Reduced predation risk for melanistic pygmy grasshoppers in post-fire environments. Ecol Evol. 2012;2:2204–12.View ArticlePubMedPubMed CentralGoogle Scholar
- Kang C, Stevens M, Moon J-Y, Lee S-I, Jablonski P. Camouflage through behavior in moths: the role of background matching and disruptive coloration. Behav Ecol. 2015;26:45–54.View ArticleGoogle Scholar
- Nachman MW, Hoekstra HE, D’Agostino SL. The genetic basis of adaptive melanism in pocket mice. Proc Natl Acad Sci U S A. 2003;100:5268–73.View ArticlePubMedPubMed CentralGoogle Scholar
- Rosenblum EB. Convergent evolution and divergent selection: lizards at the White Sands ecotone. Am Nat. 2006;167:1–15.View ArticlePubMedGoogle Scholar
- Stuart-Fox D, Moussalli A. Camouflage, communication and thermoregulation: lessons from colour changing organisms. Philos Trans R Soc Lond B Biol Sci. 2009;364:463–70.View ArticlePubMedGoogle Scholar
- Umbers KDL, Fabricant SA, Gawryszewski FM, Seago AE, Herberstein ME. Reversible colour change in Arthropoda. Biol Rev. 2014;89:820–48.View ArticlePubMedGoogle Scholar
- Stevens M. Color change, phenotypic plasticity, and camouflage. Front Ecol Evol. 2016;4:1–10.View ArticleGoogle Scholar
- Lovell PG, Ruxton GD, Langridge KV, Spencer KA. Egg-laying substrate selection for optimal camouflage by quail. Curr Biol. 2013;23:260–4.View ArticlePubMedGoogle Scholar
- Marshall KLA, Philpot KE, Stevens M. Microhabitat choice in island lizards enhances camouflage against avian predators. Sci Rep. 2016;6:19815.View ArticlePubMedPubMed CentralGoogle Scholar
- Kettlewell HBD. Recognition of appropriate backgrounds by the pale and black phases of Lepidoptera. Nature. 1955;175:943–4.View ArticlePubMedGoogle Scholar
- Ahnesjö J, Forsman A. Differential habitat selection by pygmy grasshopper color morphs; interactive effects of temperature and predator avoidance. Evol Ecol. 2006;20:235–57.View ArticleGoogle Scholar
- Houston AI, Stevens M, Cuthill IC. Animal camouflage: Compromise or specialize in a 2 patch-type environment? Behav Ecol. 2007;18:769–75.View ArticleGoogle Scholar
- Karpestam E, Merilaita S, Forsman A. Detection experiments with humans implicate visual predation as a driver of colour polymorphism dynamics in pygmy grasshoppers. BMC Ecol. 2013;13:17.View ArticlePubMedPubMed CentralGoogle Scholar
- Merilaita S, Tuomi J, Jormalainen V. Optimization of cryptic coloration in heterogeneous habitats. Biol J Linn Soc. 1999;67:151–61.View ArticleGoogle Scholar
- Bond AB, Kamil AC. Spatial heterogeneity, predator cognition, and the evolution of color polymorphism in virtual prey. Proc Natl Acad Sci U S A. 2006;103:3214–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Stevens M, Lown AE, Denton AM. Rockpool gobies change colour for camouflage. PLoS One. 2014;9:1–8.Google Scholar
- Stevens M, Lown AE, Wood LE. Color change and camouflage in juvenile shore crabs Carcinus maenas. Front Ecol Evol. 2014;2:1–14.View ArticleGoogle Scholar
- Merilaita S, Lyytinen A, Mappes J. Selection for cryptic coloration in a visually heterogeneous habitat. Proc R Soc Lond Ser B Biol Sci. 2001;268:1925–9.View ArticleGoogle Scholar
- Huyghe K, Vanhooydonck B, Herrel A, Tadic Z, Van Damme R. Morphology, performance, behavior and ecology of three color morphs in males of the lizard Podarcis melisellensis. Integr Comp Biol. 2007;47:211–20.View ArticlePubMedGoogle Scholar
- Théry M, Casas J. Predator and prey views of spider camouflage. Nature. 2002;415:133.View ArticlePubMedGoogle Scholar
- Diffendorfer JE. Testing models of source-sink dynamics and balanced dispersal. Oikos. 1998;81:417–33.View ArticleGoogle Scholar
- Pulliam H. Source, sinks, and population regulation. Am Nat. 1988;132:652–61.View ArticleGoogle Scholar
- Herder F, Pfaender J, Schliewen UK. Adaptive sympatric speciation of polychromatic “roundfin” sailfin silverside fish in Lake Matano (Sulawesi). Evolution (N Y). 2008;62:2178–95.Google Scholar
- Andersson M. Sexual selection. Princeton: Princeton Universiy Press; 1994.Google Scholar
- Langellotto GA, Denno RF, Ott JR. A trade-off between flight capability and reproduction in males of a wing-dimorphic insect. Ecology. 2000;81:865–75.View ArticleGoogle Scholar
- Denno RF, Roderick GK, Olmstead KL, Dobel HG. Density-related migration in planthoppers (Homoptera: Delphacidae): the role of habitat persistence. Am Nat. 1991;138:1513–41.View ArticleGoogle Scholar
- Novotný V. Relation between temporal persistence of host plants and wing length in leafhoppers (Hemiptera: Auchenorrhyncha). Ecol Entomol. 1994;19:168–76.View ArticleGoogle Scholar
- Terossi M, López Greco LS, Mantelatto FL. Hippolyte obliquimanus (Decapoda: Caridea: Hippolytidae): a gonochoric or hermaphroditic shrimp species? Mar Biol. 2008;154:127–35.View ArticleGoogle Scholar
- Terossi M, Mantelatto FL. Sexual ratio, reproductive period and seasonal variation of the gonochoric shrimp Hippolyte obliquimanus (Caridea: Hippolytidae). Mar Biol Res. 2010;6:213–9.View ArticleGoogle Scholar
- Merilaita S, Jormalainen V. Evolution of sex differences in microhabitat choice and colour polymorphism in Idotea baltica. Anim Behav. 1997;54:769–78.View ArticlePubMedGoogle Scholar
- Sardà F, Company JB, Costa C. A morphological approach for relating decapod crustacean cephalothorax shape with distribution in the water column. Mar Biol. 2005;147:611–8.View ArticleGoogle Scholar
- Aguzzi J, Costa C, Antonucci F, Company JB, Menesatti P, Sardá F. Influence of diel behaviour in the morphology of decapod natantia. Biol J Linn Soc. 2009;96:517–32.View ArticleGoogle Scholar
- Venables W, Ripley B. Modern applied statistics with S. 4th ed. New York: Springer; 2002.View ArticleGoogle Scholar
- Hacker S, Steneck R. Habitat architecture and the abundance and body-size-dependent habitat selection of a phytal amphipod. Ecology. 1990;71:2269–85.View ArticleGoogle Scholar
- Poore AGB, Steinbenrg PD. Preference-performance relationships and effects of host plant choice in an herbivorous marine amphipod. Ecol Monogr. 1999;69:443–64.Google Scholar
- Gamble FW, Keeble FW. Hippolyte varians: a study in colour-change. Q J Microc Sci. 1900;43:589–703.Google Scholar
- Bauer RT. Color patterns of the shrimps Heptacarpus pictus and H. paludicola (Caridea: Hippolytidae). Mar Biol. 1981;64:141–52.View ArticleGoogle Scholar
- Stevens M, Párraga CA, Cuthill IC, Partridge JC, Troscianko TS. Using digital photography to study animal coloration. Biol J Linn Soc. 2007;90:211–37.View ArticleGoogle Scholar
- Stevens M, Stoddard MC, Higham JP. Studying primate color: towards visual system-dependent methods. Int J Primatol. 2009;30:893–917.View ArticleGoogle Scholar
- Westland S, Ripamonti C. Computational color science. Chichester: John Wiley & Sons Ltd.; 2004.Google Scholar
- Rasband W. ImageJ [online]. [Internet]. National Institute of Health, Bethesda. 1997. Available from: https://imagej.nih.gov/ij/.
- Osorio D, Vorobyev M, Jones CD. Colour vision of domestic chicks. J Exp Biol. 1999;202:2951–9.PubMedGoogle Scholar
- Stevens M. Avian vision and egg colouration: concepts and measurements. Avian Biol Res. 2011;4:168–84.View ArticleGoogle Scholar
- Spottiswoode C, Stevens M. How to evade a coevolving brood parasite: egg discrimination versus egg variability as host defences. Proc R Soc B Biol Sci. 2011;278:3566–73.View ArticleGoogle Scholar
- Kelber A, Vorobyev M, Osorio D. Animal colour vision--behavioural tests and physiological concepts. Biol Rev. 2003;78:81–118.View ArticlePubMedGoogle Scholar
- Chapman BB, Hulthén K, Brönmark C, Nilsson PA, Skov C, Hansson L-A, et al. Shape up or ship out: migratory behaviour predicts morphology across spatial scale in a freshwater fish. J Anim Ecol. 2015;84:1187–93.View ArticlePubMedGoogle Scholar
- Rohlf F. TpsDig, digitize landmarks and outlines. Department of Ecology and Evolution, State University of New York at Stony Brook; 2009. Available from: http://life.bio.sunysb.edu/morph/.
- Zelditch M, Swiderski D, Sheets H, Fink W. Geometric morphometrics for biologists: a primer. New York: Elsevier Academic Press; 2004.Google Scholar
- Rohlf F. TpsRelw, relative warps analysis. Department of Ecology and Evolution, State University of New York at Stony Brook; 2008. Available from: http://life.bio.sunysb.edu/morph/.
- Hultgren KM, Mittelstaedt H. Color change in a marine isopod is adaptive in reducing predation. Curr Zool. 2015;61:739–48.View ArticleGoogle Scholar
- Stepien C. Regulation of color morphic patterns in the giant kelpfish, Heterostichus rostratus Girard: genetic versus environmental factors. J Exp Mar Biol Ecol. 1986;100:181–208.View ArticleGoogle Scholar
- Chien Y-H, Jeng S-C. Pigmentation of kuruma prawn, Penaeus japonicus Bate, by various pigment sources and levels and feeding regimes. Aquaculture. 1992;102:333–46.View ArticleGoogle Scholar
- Tlusty M, Hyland C. Astaxanthin deposition in the cuticle of juvenile American lobster (Homarus americanus): implications for phenotypic and genotypic coloration. Mar Biol. 2005;147:113–9.View ArticleGoogle Scholar
- Merilaita S, Stevens M. Crypsis through background matching. In: Stevens M, Merilaita S, editors. Anim. Camoufl. Cambridge: Cambridge University Press; 2011. p. 17–33.View ArticleGoogle Scholar
- Leite F, Turra A. Temporal variation in Sargassum biomass, Hypnea epiphytism and associated fauna. Braz Arch Biol Technol. 2003;46:665–71.View ArticleGoogle Scholar
- Tanaka MO, Leite FPP. Spatial scaling in the distribution of macrofauna associated with Sargassum stenophyllum (Mertens) Martius: analyses of faunal groups, gammarid life habits, and assemblage structure. J Exp Mar Biol Ecol. 2003;293:1–22.View ArticleGoogle Scholar
- Orth RJ, Van Montfrans J. Epiphyte-seagrass relationships with an emphasis on the role of micrograzing: a review. Aquat Bot. 1984;18:43–69.View ArticleGoogle Scholar
- Martin-Smith KM. Abundance of mobile epifauna: the role of habitat complexity and predation by fishes. J Exp Mar Biol Ecol. 1993;174:243–60.View ArticleGoogle Scholar
- Main K. Predator avoidance in seagrass meadows: prey behavior, microhabitat selection, and cryptic coloration. Ecology. 1987;68:170–80.View ArticleGoogle Scholar
- Maciá S, Robinson MP. Why be cryptic? Choice of host urchin is not based on camouflage in the caridean shrimp Gnathophylloides mineri. Acta Ethol. 2009;12:105–13.View ArticleGoogle Scholar
- Hultgren KM, Stachowicz JJ. Alternative camouflage strategies mediate predation risk among closely related co-occurring kelp crabs. Oecologia. 2008;155:519–28.View ArticlePubMedGoogle Scholar
- Booth CL. Evolutionary significance of ontogenetic colour change in animals. Biol J Linn Soc. 1990;40:125–63.View ArticleGoogle Scholar
- Padilla DK, Adolph SC. Plastic inducible morphologies are not always adaptive: the importance of time delays in a stochastic environment. Evol Ecol. 1996;10:105–17.View ArticleGoogle Scholar
- Johnsen S. Hidden in plain sight: the ecology and physiology of organismal transparency. Biol Bull. 2001;201:301–18.View ArticlePubMedGoogle Scholar
- Wickler W, Seibt U. Monogamy in Crustacea and man. Z Tierpsychol. 1981;57:215–34.View ArticleGoogle Scholar
- Baeza JA, Piantoni C. Sexual system, sex ratio, and group living in the shrimp Thor amboinensis (De Man): relevance to resource-monopolization and sex-allocation theories. Biol Bull. 2010;219:151–65.View ArticlePubMedGoogle Scholar
- Godoy EAS, Coutinho R. Can artificial beds of plastic mimics compensate for seasonal absence of natural beds of Sargassum furcatum? ICES J Mar Sci. 2002;59:111–5.View ArticleGoogle Scholar
- Jormalainen V, Merilaita S. Differential predation on sexes affects colour polymorphism of the isopod Idotea baltica (Pallas). Biol J Linn Soc. 1995;55:45–68.View ArticleGoogle Scholar