- Research article
- Open Access
Population expansion in the North African Late Pleistocene signalled by mitochondrial DNA haplogroup U6
© Pereira et al; licensee BioMed Central Ltd. 2010
Received: 21 September 2010
Accepted: 21 December 2010
Published: 21 December 2010
The archaeology of North Africa remains enigmatic, with questions of population continuity versus discontinuity taking centre-stage. Debates have focused on population transitions between the bearers of the Middle Palaeolithic Aterian industry and the later Upper Palaeolithic populations of the Maghreb, as well as between the late Pleistocene and Holocene.
Improved resolution of the mitochondrial DNA (mtDNA) haplogroup U6 phylogeny, by the screening of 39 new complete sequences, has enabled us to infer a signal of moderate population expansion using Bayesian coalescent methods. To ascertain the time for this expansion, we applied both a mutation rate accounting for purifying selection and one with an internal calibration based on four approximate archaeological dates: the settlement of the Canary Islands, the settlement of Sardinia and its internal population re-expansion, and the split between haplogroups U5 and U6 around the time of the first modern human settlement of the Near East.
A Bayesian skyline plot placed the main expansion in the time frame of the Late Pleistocene, around 20 ka, and spatial smoothing techniques suggested that the most probable geographic region for this demographic event was to the west of North Africa. A comparison with U6's European sister clade, U5, revealed a stronger population expansion at around this time in Europe. Also in contrast with U5, a weak signal of a recent population expansion in the last 5,000 years was observed in North Africa, pointing to a moderate impact of the late Neolithic on the local population size of the southern Mediterranean coast.
Despite much recent research, the archaeology of North Africa remains enigmatic, with questions of population continuity versus discontinuity taking centre-stage. Key issues concern the identity of the bearers of the Middle Palaeolithic (or Middle Stone Age) Aterian industry (the age of which has recently risen dramatically from ~40-20,000 years ago (ka) to ≥115-40 ka [1, 2]), and whether or not there was continuity between these and the later Upper Palaeolithic populations of the Maghreb. This question has become more urgent with the discovery that the Aterian is associated in Northwest Africa with a very early appearance of evidence for behavioural modernity, such as perforated Nassarius shell beads, use of ochre and bone tools, and long-distance exchange networks - preceding those of southern Africa and making it likely that the Aterian was carried by anatomically modern (rather than archaic) humans . The fate of the populations using this industry, and their possible connection with others in Africa and with the group who dispersed out of Africa ~60 ka to populate the rest of the world, has naturally become a question of great interest.
Further debates have focused on the question of population replacement in the late Pleistocene and Holocene. The earliest Upper Palaeolithic industry in North Africa is the Dabban, limited to Cyrenaïca (a likely glacial refuge area ) and most likely dating from ~36-50 ka to ~20 ka . Its similarities to Near Eastern Early Upper Palaeolithic industries have suggested an origin in the Levant , rather than locally in the Aterian, and similar blade industries have been found further south by 30 ka . The Dabban was replaced by the Eastern Oranian (or Eastern Iberomaurusian) in Cyrenaïca possibly as early as ~18 ka , by which time the Upper Palaeolithic had extended eastwards for the first time along the coastal belt of the Maghreb, with the Iberomaurusian (or Oranian), the origins of which are similarly controversial, especially as it appears earliest in the Northwest. It began at the Last Glacial Maximum (LGM), ~22-20 ka, with a period of intensification in the Late Glacial ~15-13 ka, although recent work suggests a possible underlying industry as early as ~26 ka [1, 6]. Similar debates concerning continuity versus replacement surround discussion of the widespread Epi-Palaeolithic Capsian industries, which saw expansion southwards from eastern Algeria into an increasingly humid Sahara in the early Holocene and sometimes associated with more gracile Mediterranean skeletal remains, and the subsequent emergence of the Neolithic [4, 7–10].
The phylogeographic analysis of human mitochondrial DNA (mtDNA) has the potential to address questions such as these . In particular, the mtDNA haplogroup U6 has a unique and highly distinctive distribution amongst human mitochondrial DNA lineages. It is found primarily in North Africa and the Canary Islands (albeit with secondary dispersals into Iberia and East Africa), with its highest frequency amongst Algerian Berbers (28%), and it has therefore been proposed to be linked to the ancestors of the indigenous Berber-speaking populations of North Africa [12–15]. Macaulay et al.  described U6 and its sister clade U5 as having evolved from a common ancestor in the Near East, approximately 50 ka; while U5 spread along the northern Mediterranean coast with the European Early Upper Palaeolithic, U6 dispersed along the southern coast, as far as Cyrenaïca, alongside the Dabban industry, ~40-50 ka, with a further expansion into Northwest Africa with the Iberomaurusian culture, ~22 ka. On this view, U6 evolved en route or within North Africa (as U5 evolved within Europe ), the presence of occasional derived U6 lineages in the Near East would signal more recent gene flow from North Africa .
These early studies were based only on information from hypervariable region I (HV-I) in the mtDNA control region and a small number of diagnostic RFLPs in the coding region, assayed in samples from several populations of North Africa and Iberia. In most of these regions, U6 frequencies are ~10% or less , and even appear absent from some Berber communities in Tunisia [18, 19]. The major sub-haplogroup U6a (characterized by control-region transitions from the ancestor of haplogroup U at nucleotide positions 16172, 16219 and 16278) is highly dispersed, occurring throughout North Africa (and at low levels in the Near East and Iberia), and a further nested subclade, U6a1 (characterized by an additional transition at position 16189), follows a similar distribution.
By contrast, U6b (characterized by variants at 16172-16219-16311) has a more limited range to the northwest of North Africa, the north of the Iberian Peninsula and, as a nested derivative (U6b1, characterized by a transition at position 16163), in the Canary Islands . In particular, the U6b1 lineage in the Canary Islands has been considered a founder lineage for the colonization of this archipelago by the Guanches (culturally very similar to Northwest Africans), ~2-3 ka , a conclusion supported by studies of ancient DNA . Hence its arrival suggests itself as a potentially useful calibration point for the mtDNA molecular clock, although the archaeological evidence for the colonisation time is rather insubstantial . In fact, the absence of U6b1 lineages anywhere outside of the Canary Islands (the few exceptions detected in Spain and in Americas being most readily explained as recent migrants from there), and the failure to detect immediate ancestors in North Africa, seem to point to the emergence of this clade within the Canary Islands - although probably soon after their colonization, as it is observed across several islands.
Maca-Meyer et al.  performed the first study of complete U6 mtDNA sequences (with 14 samples), defining a new U6 sub-haplogroup, U6c (characterized by the HV-I transition motif 16169-16172-16189), which was even more geographically restricted than U6b - limited to the west of North Africa and, as a derivative (U6c1, with an additional 16129 substitution), in the Canary archipelago. Using coding-region age estimates as maximum limits for radiation times, they proposed that the proto-U6 spread from the Near East to North Africa ~30 ka, alongside the Iberomaurusian industry, with U6a reflecting an African re-expansion from the Maghreb eastwards in Palaeolithic times, and U6a1 a further reverse movement from East Africa back to the Maghreb, possibly coinciding with the probable Afroasiatic linguistic expansion. The clades U6b and U6c, restricted to West Africa, had more localized expansions; they argued that U6b reached Iberia at the time of the diffusion of the Capsian culture in North Africa.
However, a larger study by Olivieri et al.  was closer to the earlier interpretation of Macaulay et al. . They confirmed the origin of U6, or at least that of its immediate ancestor, in southwest Asia, with an ancient introduction (alongside haplogroup M1, and the Dabban industry) to North Africa via the Levant, possibly during the Greenland Interstadial 12, from ~44-48 ka. They reaffirmed that the various U6 sub-groups originated in the southern Mediterranean area, dispersing subsequently to East Africa.
Coalescence time estimates for U6 and it subclades have varied considerably amongst these studies. Yet these are critical for studies of prehistoric dispersals, since reliable estimates can bracket the timing of demographically significant events. For example, a regionally-specific clade may have arisen from a migration event on the edge in the tree leading to that clade, and if the diversification has then arisen in situ rather than prior to the presumed founder event, the estimate of the time to the most recent common ancestor (TMRCA) can provide a minimum bound on the age of the migration event (motivating the "founder analysis" ). However, success rests on a number of requirements, principally that the phylogeny can be well-estimated and the molecular clock that converts genetic differences into time depth is well-calibrated . Considerable progress has recently been made on both of these fronts by more sophisticated analyses of the richer data source provided by mtDNA complete sequences.
With respect to the molecular clock, there are many factors leading to uncertainty. There is the wide variation in positional mutation rates and violations of the independence of mutations at different positions. Obvious regions are the paired stems of rRNAs and tRNAs , which some authors remove from the analysis , but there are other locations in the mtDNA molecule which can also present a secondary structure related with a functional role . There is the problem of multiple hits and saturation, leading to the curious observation that the total proportion of control-region polymorphisms in the African branches of the tree is lower than in the non-African ones. Selection is also an important issue, with a higher frequency of replacement substitutions in the younger branches of the human mtDNA phylogeny compared to the more internal branches [29–31]. Kivisild et al.  advocated the use of only synonymous diversity for estimation of the TMRCA, which is problematic for age estimations in young lineages, while Soares et al.  implemented a correction for the purifying selection effect on the mutation rate estimated for the entire molecule.
The choice of calibration points is also an important issue. Traditionally, an outgroup is used, where the split time with the human lineage can be assigned in some way. For humans, the closest one is that corresponding to the human-chimpanzee split, for which the fossil evidence is controversial and which is in a time frame very distant from the TMRCA of the mtDNA of Homo sapiens, rendering the application of a strict clock problematic . One recent analysis additionally used the chronometric ages of the available Neanderthal sequences as calibration points . A strategy of multiple calibration points in conjunction with relaxed-clock methods, where the rate is allowed to vary among branches in the tree  is appealing, but this has been hard to implement in the human tree because of unavailability of secure multiple calibration points. Bandelt et al.  advocate that calibrated radiocarbon dates in favourable pioneer-settlement situations with a well-defined founder mtDNA scenario and a rich archaeological record could be used for calibration purposes, but consensus for both radiocarbon dates and founder mtDNA lineages are far from being achieved in most known settlement situations. Endicott and Ho  applied an internal calibration to the human mtDNA tree by specifying priors on the ages of three nodes in the tree associated with demographic signals: the entry into Australia and New Guinea by establishing a minimum of 40 ka for haplogroup P; and the post-Last Glacial Maximum expansion of haplogroups H1 and H3 (unfortunately suggested as 18 ka). This internal calibration was performed using a Bayesian approach with the software BEAST , and resulted in a substitution rate 1.4 times higher than that resulting from the human-chimpanzee calibration. BEAST also, however, allows a reconstruction of effective population size through time, by using the Bayesian skyline plot (BSP ), based on a coalescent model analysed by Markov Chain Monte Carlo sampling. BSPs do not require a pre-specified parametric growth model as do other methods and, although designed for use with population data, they have also been used to attempt parameter estimation from haplogroup data with some apparent success .
Here, we analyse an additional 39 complete U6 genomes, from the full range of the U6 geographic distribution, including the Near East, Iberia and the Canary Islands. This has enabled us to construct a phylogenetic tree including 89 U6 genomes in total, and to re-evaluate the demographic history of the haplogroup, and its role in North African prehistory, in the light of the recent developments in the calibration of mutation rates. A comparison tree was inferred for 141 U5 sequences from the literature, allowing us to test the use of four alternative internal calibration points (the settlement of the Canary Islands, the settlement of Sardinia and its internal population re-expansion, and the split between haplogroups U5 and U6 around the time of the first modern human settlement of the Near East) against the recently developed complete genome clock with a correction for purifying selection.
Based on information from HV-I that allowed the general classification into haplogroups, we selected 39 U6 individuals for complete sequencing: 22 from Portugal, most of them included in a published Portuguese database  and some new data; 5 from the Canary Islands; 1 from Morocco; 2 from a sample of 583 Ashkenazi Jews (of Polish and Russian ancestry ), 7 from 1143 non-Ashkenazi Jews (of Turkish, Bulgarian, Moroccan, Tunisian, and Ethiopian ancestry), and 2 from 253 Near Eastern Palestinians. Except in the case of the Canary Islands dataset, where we selected 5 U6b1 samples randomly, all the available U6 samples were sequenced from each of these datasets. The samples belonged to unrelated individuals, who gave informed consent for their biological samples to be used for mtDNA characterisation. The work complied with the Helsinki Declaration of Ethical Principles (59th WMA General Assembly, Seoul, October 2008) and was approved by IPATIMUP Ethics Commission.
Complete mtDNA sequencing and nomenclature
We amplified mtDNA using 32 overlapping fragments as described elsewhere . After purification, we used the forward primers for the sequencing, and in some cases, also the reverse primers (in the presence of polycytosine stretches, when polymorphisms A574C and T16189C occur). We performed sequencing on a 3100 DNA Analyzer (AB Applied Biosystems, Foster City, CA), and the resulting sequences were read with SeqScape (AB Applied Biosystems, Foster City, CA) and BioEdit version 22.214.171.124 , by two independent investigators. In cases of ambiguous base calls, the PCR and sequencing reactions were repeated. Furthermore, the protocols for rechecking both haplogroup-defining polymorphisms, previously well-established in the literature, as well as private mutations were followed . Mutations were scored relative to the revised reference sequence, rCRS , and numbers 1-16569 refer to the position of the mutation in that sequence. The 39 complete mtDNA sequences have been deposited in GenBank (Accession Numbers HQ651676-HQ651714).
For the U6 phylogeny reconstruction, besides the new 39 sequences obtained here, we used 50 complete sequences previously published [22, 23, 40, 44–47], and some unpublished sequences deposited in GenBank from the Family Tree DNA Company (accession numbers provided in Additional File 1). We also inferred a U5 tree, from 141 complete sequences available in GenBank and listed in Additional File 1. Preliminary network analyses  led to a suggested branching order for the trees, which we then constructed most parsimoniously by hand. The software mtDNA-GeneSyn  was used to convert files.
For the first point, we assigned a mode age of 45 ka and a 95th percentile of 60 ka for the split between the U5 and U6 lineages, based on the hypothesis that some of the first settlers of Europe  were carrying haplogroup U that would later evolve into U5 . Considering this, the age of the U5-U6 split would be a minimum of 45 ka old. Reliable archaeological dates are minimum estimates of a first settlement, justifying the use of the exponential distribution.
The second calibration point with mode 2.3 ka and 95th percentile 5 ka was the age of the U6b1 branch based on the archaeological date for the colonization of the Canary Islands. The first settlement of the Canary Islands is extremely uncertain and the archaeology of the islands is not extensive . Furthermore, the fact that U6b is so uncommon in North Africa could mean that the populations that carried U6b into the Canary Islands disappeared by drift and U6b1 already existed outside the Canaries. Again an exponential distribution was used to capture this uncertainty.
The third point with mode 8 ka and 95th percentile 14 ka was within U5b3a1, a subclade of which, U5b3a1a, is found only on Sardinia . Sardinia was permanently colonized at least 8 ka . The colonization time most probably took place between the radiation of the Sardinian-specific branch (U5b3a1a) and its split point with mainland Europe (U5b3a1), indicating that the latter is necessarily higher than 8 ka . Again the age of the clade may be substantially higher than 8 ka and therefore an exponential distribution was used.
The fourth calibration point we used was an age estimate for the Sardinian U5b3a1a branch, assigned a normal distribution (truncated at zero) centred on 5.8 ka, with a standard deviation of 1,000 years. After the settlement of Sardinia, the population size was probably low (considering the long branch between U5b3a1 and the ancestor of U5b3a1a). It is tempting to identify the subsequent rather star-like radiation of U5b3a1a with a population expansion. The late Neolithic in Sardinia around 5-6 ka was a likely time of internal re-expansion and population increase  and we hypothesized that the age of U5b3a1a corresponds to this internal expansion. This interpretation gains strength from a signal elsewhere in the phylogeny: haplogroup M1 in Sardinia also presents a rather star-like clade  dating to 5.2 ka, based on ρ and the time-dependent clock.
BEAST uses a Markov-chain Monte-Carlo (MCMC) approach to sample from the posterior distributions of model parameters (branching times in the tree and substitution rates). Specifically, we ran 150,000,000 iterations, with samples drawn every 1,000 MCMC steps, after a discarded burn-in of 15,000,000 steps. We checked for convergence to the stationary distribution and sufficient sampling by inspection of posterior samples. We also obtained Bayesian skyline plots from BEAST and visualised them with Tracer v1.3 from posterior distributions of parameters run for 50,000,000 iterations (with samples drawn every 1,000 MCMC steps, after a discarded burn-in of 5,000,000 steps). We used a generation time of 25 years to rescale the vertical axis of the BSP to years. In addition, we forced the larger sub-haplogroups (U6abd, U6a, U6bd, U6b, U6c and U6d) to be monophyletic in the analysis, as the presence of fast-evolving positions (such as 16189 and 16311) leads to the reconstruction of diverse phylogenies, which would not be comparable with the one inferred using network analysis; MCMC updates which violated this assumption were immediately rejected.
To determine and visualise the geographical distribution of U6, U6a and U6bd, we constructed interpolation maps using the "Spatial Analyst Extension" of ArcView version 3.2 http://www.esri.com/software/arcview/. We used the "Inverse Distance Weighted" (IDW) option with a power of two for the interpolation of the surface. IDW assumes that each input point has a local influence that decreases with distance. The geographic location used is the centre of the distribution area from which the individual samples of each population were collected. The data used are listed in Additional File 1.
Results and Discussion
The enlarged U6 phylogeny (Additional File 2) confirmed the principal sub-groups already identified , and did not reveal any novel ones. It highlights, however, that variation in HV-I can be misleading in regard to the branching structure, particularly when major splits in the phylogeny are supported only by mutations at hotspot positions, justifying Olivieri et al.'s caution in the naming of such clusters (their Fig. three). For example, the additional data now allows transitions at position 16189 in U6 to now be resolved into several events , showing that the old classification of "U6a1" based only on HV-I diversity , and unifying U6a1 with U6a2 and U6a3 in Olivieri et al.  is not reliable. The case for the postulated "U6a1" movement from East Africa back to the Maghreb advanced by Maca-Meyer et al.  is not favoured by our inferred phylogeny, as the 16189 transition does not identify non-monophyletic groups. Indeed, the only sub-clade which seems to have a preferred distribution in East Africa (three individuals from Ethiopia) falls within U6a2, with only coding-region diagnostic mutations at positions 6359 and 11204 (dating to 13.4 ± 4.0 ka).
Age estimates for U6 and its major subgroups.
The posterior medians and 95% intervals for the ages of the points used for calibration were: for the U5-U6 split, 46,222 years [45,000-50,293]; U5b3a1, 9,825 years [8,000-12,714]; U5b3a1a, 5,850 years [4,953-6,743]; and U6b1, 4,835 years [2,958-7,064]. The posterior distributions for the oldest points agree better with the prior dates than the youngest point of U6b1, indicating that the Bayesian estimates will readapt the priors according to the existing information in the tree. This is most obvious in the case of the age of U6b1, suggesting that either the first settlement of the Canary Archipelago was earlier than the (admittedly weak) existing archaeological evidence indicates  or that U6b1 had already arisen prior to colonisation and has since drifted to extinction in the northwest African source, or at least has yet to be sampled. The estimates using the rate of Soares et al.  are both somewhat younger, suggesting that the Bayesian calibration (with only one calibration point in this range) may be failing to correct adequately for purifying selection at this time depth.
It is also worth pointing out that the age of haplogroup U reported here (the U5-U6 split at around 46 ka) is slightly lower than the age of U reported recently using ρ and ML and the complete sequence clock corrected for purifying selection [11, 31] which tended to be > 50 ka. In fact, a ρ estimate of 44,145 years [32,460-56,254] is obtained using only the U5-U6 data with the Soares et al. rate; so the age of U in the Bayesian analysis would most probably have been higher if all the sub-branches of U had been included. The ages of U5b3a1 and U5b3a1a were 11,912 [3,456-20,777] years and 4,128 years [2,016-6,269] respectively. The comparison of the posterior mean for the ages in Bayesian with the ρ estimates for the four points indicate a mean ratio of 1.1 higher in the Bayesian analysis, in contrast to the trend obtained in the U6 ages described above. The Bayesian and ρ estimates overlap.
A further check of the clock is the age of U5a2a , since a single HV-I sequence belonging to this clade has been obtained from an ancient skeletal sample dated by radiocarbon to 7.8 ka . The date obtained for U5a2a with the internal calibration was 8,045 years [4,391-13,184], concordant with the minimum possible age of the clade.
The BSP for U5 shows a small effective population size from the time of its origin until a strong expansion led to a ~11-fold increase within a period of ~6 ka in the late Pleistocene/early Holocene (14.3-8.3 ka). Following this, the effective size again remained more or less constant till a second strong expansion occurred, leading to an increase of ~5-fold after 4.3 ka (we took the year 1600AD as the most recent time-point ). For U6, the pattern is slightly more complex. The initial effective size was somewhat larger (~1.6 times) compared with U5, and the initial expansion begins earlier, ~22.2 ka, with a more gradual expansion until ~10.2 ka, leading to a 3-fold increase. As with U5, there is a further expansion in the Neolithic, after 4.8 ka with a shallower increase of ~1.5-fold.
The Bayesian evolutionary analyses conducted in this work, as well as the comparison between recently updated mutation rates and the improvement of the phylogenetic resolution for the mitochondrial haplogroup U6, enable us to revise our interpretation of the evolutionary history of haplogroup U6 and sketch a demographic prehistory for North Africa, which we can then compare with that of Europe. In particular, they allow us to address many of the archaeological questions raised in the introduction.
The recently revised archaeological dates for the Aterian industry of North Africa emphasize that the makers of this industry do not appear to have left any imprint in the maternal lineages of present-day North Africans. The oldest arrivals amongst extant mtDNAs appear to be the U6 and M1 lineages, which date to 36.6 (24.9; 48.8) and 25.4 (17.9; 33.1) ka respectively . As with U5 in Europe , the arrival time could be older in each case, since the haplogroups appear likely to have arisen within the southern Mediterranean region from haplogroup U and M ancestors, making dating the arrival time very imprecise. Nevertheless, the estimates seem to match best the appearance of the Upper Palaeolithic Dabban industry in Cyrenaïca, as suggested before [15, 23].
There is an intriguing further signal in the U6 data, witnessed by the Bayesian skyline plot. For the European haplogroup U5, which is one of the most ancient in Europe , we identified a strong expansion (an ~11-fold increase in effective population size) occurring in the Lateglacial period between the LGM and the beginning of the Holocene, followed by another large population expansion (~5-fold) after 5 ka, evidently associated with late Neolithic/early Bronze Age (rather than, for example, the early Neolithic expansion in Europe, which began ~8.5 ka). For U6, by contrast, the corresponding increases in effective sizes were less marked (~3-fold and ~1.5-fold, respectively), and the signal indicates that the expansion began earlier, ~22 ka. This coincides closely with the beginning of the Iberomaurusian industry in the Maghreb. These results therefore suggest that the Iberomaurusian was initiated by an expansion of modern humans of ultimately Near Eastern, carrying mtDNA haplogroup U6, who had spread into Cyrenaïca ~35-45 ka and produced the Dabban industry. The link back to the Near East and the European Early Upper Palaeolithic (which likely has the same source) may explain the suggested skeletal similarities between the robust Iberomaurusian "Mechta-Afalou" burials and European Cro-Magnon remains, as well as the case for continuity of the bearers of the Iberomaurusian industry from Morocco with later northwest African populations suggested by the dental evidence .
We can compare the U5 and U6 BSPs with the ones for geographic regions published in Atkinson et al. , inferred from a more random sample of mtDNA sequences observed in those regions - for which the model behind the BSP is, on the surface, a better match. Similar to the situation reported for sub-Saharan Africa , the picture that emerges from U5 and U6 appears to represent well the general demographic patterns observed in, respectively, Europe and North Africa (although the latter was combined the Near East).
This observation suggests that by investigating in depth U6 and U5, the oldest lineages present in North Africa and Europe, respectively, we are indeed receiving signals from the demographic pre-history of modern humans in these regions.
Aside from U6, North Africa was also the recipient of European, Near Eastern and sub-Saharan African lineages most of which most likely arrived in the Holocene. Haplogroups H1, H3 and V expanded in Iberia in the Lateglacial/postglacial [11, 58–61], and evidently spread into North Africa from Iberia across the Gibraltar Straits, most likely in the early Holocene [62–65]. Although the postglacial Capsian industry appears to have originated in eastern Algeria, it is tempting to hypothesize a connection with the arrival of these new populations from southwest Europe. Intriguingly, although U5b1, which also expanded from southwest Europe in the Lateglacial, has not been seen in Moroccan Berbers, it has been identified amongst Algerian Berbers and Fulbe from Senegal, as well as Iberia, Italy and northern Eurasian Saami and Yakut .
Most sub-Saharan lineages observed in North Africa are presently difficult to date and probably arrived at various times, but the age of the sub-Saharan subclade L3e5 indicates its arrival in North Africa from the south ~7 ka, following its expansion in the immediate postglacial humid phase ~11.5 ka . Other L3 lineages seem to have been introduced even in more recent times, during the slave trade initiated by the Arab conquest of North Africa . The Near Eastern haplogroups J and T (and probably K) appear to be concentrated more towards the east , mirroring the higher densities of U6, H and V in the west . These may reflect the spread of the Neolithic into North Africa from the Levant, but their phylogeography awaits detailed analysis.
FCT, the Portuguese Foundation for Science and Technology, supported this work through the research project PTDC/ANT/66275/2006 and the personal grants to VF (SFRH/BD/61342/2009), JBP (SFRH/BD/45657/2008), MDC (SFRH/BD/48372/2008), HM (SFRH/BD/44089/2008) and PS (SFRH/BPD/64233/2009). IPATIMUP is an Associate Laboratory of the Portuguese Ministry of Science, Technology and Higher Education and is partially supported by FCT. PS was also supported by a Marie Curie Early Stage Training Grant. We thank Sturla Ellingvåg (of Explico) for the Canarian samples.
- Barton RNE, Bouzouggar A, Bronk-Ramsey C, Collcutt SN, Higham TFG, Humphrey LT, Parfitt S, Rhodes EJ, Schwenninger JL, Stringer CB, Turner E, Ward S: Abrupt climatic change and chronology of the Upper Palaeolithic in northern and eastern Morocco. Rethinking the human revolution: New behavioural & biological perspectives on the origins and dispersal of modern humans. Edited by: Mellars P, Boyle K, Bar-Yosef O, Stringer C. 2007, Cambridge: Macdonald Institute Monographs, 177-186.Google Scholar
- Barton RNE, Bouzouggar A, Collcutt SN, Schwenninger J-L, Clark-Balzan L: OSL dating of the Aterian levels at Grotte de Dar es-Soltan I (Rabat, Morocco) and possible implications for the dispersal of modern Homo sapiens. Quaternary Sci Rev. 2009, 28: 1914-1931. 10.1016/j.quascirev.2009.03.010.View ArticleGoogle Scholar
- Bouzouggar A, Barton RNE, Vanhaeren M, D'Errico F, Collcutt S, Higham TFG, Hodge R, Parfitt S, Rhodes E, Schwenninger J-L, Stringer C, Turner E, Ward S, Moutmir A, Stambouli A: 82,000-year-old shell beads from North Africa and implications for the origins of modern human behaviour. Proc Natl Acad Sci USA. 2007, 104: 9964-9969. 10.1073/pnas.0703877104.PubMed CentralView ArticlePubMedGoogle Scholar
- Barker G: Transitions to farming and pastoralism in North Africa. Examining the farming/language dispersal hypothesis. Edited by: Bellwood P, Renfrew C. 2002, Cambridge: McDonald Institute Monographs, 151-161.Google Scholar
- Moyer CC: The organisation of lithic technology in the Middle and Early Upper Palaeolithic industries at the Haua Fteah, Libya. PhD thesis. 2004, University of CambridgeGoogle Scholar
- Bouzouggar A, Barton RNE, Blockley S, Bronk-Ramsey C, Collcutt SN, Gale R, Higham TFG, Humphrey LT, Parfitt S, Turner E, Ward S: Reevaluating the Age of the Iberomaurusian in Morocco. Afr Archaeol Rev. 2008, 25: 3-19. 10.1007/s10437-008-9023-3.View ArticleGoogle Scholar
- Rahmani N: Technological and cultural change among the last hunter-gatherers of the Maghreb: the Capsian (10,000-6000 BP). J World Prehist. 2004, 18: 57-105. 10.1023/B:JOWO.0000038658.50738.eb.View ArticleGoogle Scholar
- Jackes M, Lubell D: Early and Middle Holocene Environments and Capsian Cultural Change: Evidence from the Telidjene Basin, Eastern Algeria. Afr Archaeol Rev. 2008, 25: 41-55. 10.1007/s10437-008-9024-2.View ArticleGoogle Scholar
- Barich BE, Garcea EAA: Ecological Patterns in the Upper Pleistocene and Holocene in the Jebel Gharbi, Northern Libya: chronology, climate and human Occupation. Afr Archaeol Rev. 2008, 25: 87-97. 10.1007/s10437-008-9020-6.View ArticleGoogle Scholar
- Barker G: The agricultural revolution in prehistory. 2006, Oxford: Oxford University PressGoogle Scholar
- Soares P, Achilli A, Semino O, Davies W, Macaulay V, Bandelt H-J, Torroni A, Richards MB: The archaeogenetics of Europe. Curr Biol. 2010, 20: R174-183. 10.1016/j.cub.2009.11.054.View ArticlePubMedGoogle Scholar
- Rando JC, Pinto F, González AM, Hernández M, Larruga JM, Cabrera VM, Bandelt H-J: Mitochondrial DNA analysis of northwest African populations reveals genetic exchanges with European, near-eastern, and sub-Saharan populations. Ann Hum Genet. 1998, 62: 531-550. 10.1046/j.1469-1809.1998.6260531.x.View ArticlePubMedGoogle Scholar
- Rando JC, Cabrera VM, Larruga JM, Hernández M, González AM, Pinto F, Bandelt H-J: Phylogeographic patterns of mtDNA reflecting the colonization of the Canary Islands. Ann Hum Genet. 1999, 63: 413-428. 10.1046/j.1469-1809.1999.6350413.x.View ArticlePubMedGoogle Scholar
- Corte-Real HB, Macaulay VA, Richards MB, Hariti G, Issad MS, Cambon-Thomsen A, Papiha S, Bertranpetit J, Sykes BC: Genetic diversity in the Iberian Peninsula determined from mitochondrial sequence analysis. Ann Hum Genet. 1996, 60: 331-350. 10.1111/j.1469-1809.1996.tb01196.x.View ArticlePubMedGoogle Scholar
- Macaulay V, Richards M, Hickey E, Vega E, Cruciani F, Guida V, Scozzari R, Bonne-Tamir B, Sykes B, Torroni A: The emerging tree of West Eurasian mtDNAs: a synthesis of control-region sequences and RFLPs. Am J Hum Genet. 1999, 64: 232-249. 10.1086/302204.PubMed CentralView ArticlePubMedGoogle Scholar
- Richards M, Rengo C, Cruciani F, Gratrix F, Wilson JF, Scozzari R, Macaulay V, Torroni A: Extensive female-mediated gene flow from sub-Saharan Africa into near eastern Arab populations. Am J Hum Genet. 2003, 72: 1058-1064. 10.1086/374384.PubMed CentralView ArticlePubMedGoogle Scholar
- Pereira L, Cunha C, Alves C, Amorim A: African female heritage in Iberia: a reassessment of mtDNA lineage distribution in present times. Hum Biol. 2005, 77: 213-229. 10.1353/hub.2005.0041.View ArticlePubMedGoogle Scholar
- Cherni L, Loueslati BY, Pereira L, Ennafaa H, Amorim A, El Gaaied AB: Female gene pools of Berber and Arab neighboring communities in central Tunisia: microstructure of mtDNA variation in North Africa. Hum Biol. 2005, 77: 61-70. 10.1353/hub.2005.0028.View ArticlePubMedGoogle Scholar
- Loueslati BY, Cherni L, Khodjet-Elkhil H, Ennafaa H, Pereira L, Amorim A, Ben Ayed F, Ben Ammar Elgaaied A: Islands inside an island: reproductive isolates on Jerba island. Am J Hum Biol. 2006, 18: 149-153. 10.1002/ajhb.20473.View ArticlePubMedGoogle Scholar
- Maca-Meyer N, Arnay M, Rando JC, Flores C, González AM, Cabrera VM, Larruga JM: Ancient mtDNA analysis and the origin of the Guanches. Eur J Hum Genet. 2004, 12: 155-162. 10.1038/sj.ejhg.5201075.View ArticlePubMedGoogle Scholar
- Galvén Santos B, Hernández Gómez CM, Alberto Barroso V, Barro A, Eugenio CM, Matos L, Machado C, Rodríguez A, Febles JV, Rivero D: Poblamiento prehistórico en la costa de Buena Vista del Norte (Tenerife). El conjunto arqueológico Fuente-Arena. Investigaciones Arqueológicas. 1999, 6: 9-258.Google Scholar
- Maca-Meyer N, Gonzalez AM, Pestano J, Flores C, Larruga JM, Cabrera VM: Mitochondrial DNA transit between West Asia and North Africa inferred from U6 phylogeography. BMC Genet. 2003, 4: 15-10.1186/1471-2156-4-15.PubMed CentralView ArticlePubMedGoogle Scholar
- Olivieri A, Achilli A, Pala M, Battaglia V, Fornarino S, Al-Zahery N, Scozzari R, Cruciani F, Behar DM, Dugoujon JM, Coudray C, Santachiara-Benerecetti AS, Semino O, Bandelt H-J, Torroni A: The mtDNA legacy of the Levantine early Upper Paleolithic in Africa. Science. 2006, 314: 1767-1770. 10.1126/science.1135566.View ArticlePubMedGoogle Scholar
- Richards M, Macaulay V, Hickey E, Vega E, Sykes B, Guida V, Rengo C, Sellitto D, Cruciani F, Kivisild T, Villems R, Thomas M, Rychkov S, Rychkov O, Rychkov Y, Gölge M, Dimitrov D, Hill E, Bradley D, Romano V, Calì F, Vona G, Demaine A, Papiha S, Triantaphyllidis C, Stefanescu G, Hatina J, Belledi M, Di Rienzo A, Novelletto A, Oppenheim A, Nørby S, Al-Zaheri N, Santachiara-Benerecetti S, Scozari R, Torroni A, Bandelt H-J: Tracing European founder lineages in the Near Eastern mtDNA pool. Am J Hum Genet. 2000, 67: 1251-1276.PubMed CentralView ArticlePubMedGoogle Scholar
- Bandelt H-J, Kong Q-P, Yao Y-G, Richards M, Macaulay V: Estimation of mutation rates and coalescence times: some caveats. Mitochondrial DNA and the Evolution of Homo sapiens. Edited by: Bandelt H-J, Macaulay V, Richards M. 2006, Berlin: Springer-Verlag, 47-90.Google Scholar
- Pereira L, Freitas F, Fernandes V, Pereira JB, Costa MD, Costa S, Máximo V, Macaulay V, Rocha R, Samuels DC: The diversity present in 5140 human mitochondrial genomes. Am J Hum Genet. 2009, 84: 628-640. 10.1016/j.ajhg.2009.04.013.PubMed CentralView ArticlePubMedGoogle Scholar
- Endicott P, Ho SY: A Bayesian evaluation of human mitochondrial substitution rates. Am J Hum Genet. 2008, 82: 895-902. 10.1016/j.ajhg.2008.01.019.PubMed CentralView ArticlePubMedGoogle Scholar
- Pereira F, Soares P, Carneiro J, Pereira L, Richards MB, Samuels DC, Amorim A: Evidence for variable selective pressures at a large secondary structure of the human mitochondrial DNA control region. Mol Biol Evol. 2008, 25: 2759-2770. 10.1093/molbev/msn225.View ArticlePubMedGoogle Scholar
- Elson JL, Turnbull DM, Howell N: Comparative genomics and the evolution of human mitochondrial DNA: assessing the effects of selection. Am J Hum Genet. 2004, 74: 229-238. 10.1086/381505.PubMed CentralView ArticlePubMedGoogle Scholar
- Kivisild T, Shen P, Wall DP, Do B, Sung R, Davis K, Passarino G, Underhill PA, Scharfe C, Torroni A, Scozzari R, Modiano D, Coppa A, de Knijff P, Feldman M, Cavalli-Sforza LL, Oefner PJ: The role of selection in the evolution of human mitochondrial genomes. Genetics. 2006, 172: 373-387. 10.1534/genetics.105.043901.PubMed CentralView ArticlePubMedGoogle Scholar
- Soares P, Ermini L, Thomson N, Mormina M, Rito T, Röhl A, Salas A, Oppenheimer S, Macaulay V, Richards MB: Correcting for purifying selection: an improved human mitochondrial molecular clock. Am J Hum Genet. 2009, 84: 740-759. 10.1016/j.ajhg.2009.05.001.PubMed CentralView ArticlePubMedGoogle Scholar
- Endicott P, Ho SY, Metspalu M, Stringer C: Evaluating the mitochondrial timescale of human evolution. Trends Ecol Evol. 2009, 24: 515-521. 10.1016/j.tree.2009.04.006.View ArticlePubMedGoogle Scholar
- Endicott P, Ho SY, Stringer C: Using genetic evidence to evaluate four palaeoanthropological hypotheses for the timing of Neanderthal and modern human origins. J Hum Evol. 2010, 59: 87-95. 10.1016/j.jhevol.2010.04.005.View ArticlePubMedGoogle Scholar
- Drummond AJ, Ho SYW, Phillips MJ, Cooper A: Relaxed phylogenetics and dating with confidence. PLoS Biol. 2006, 4: e88-10.1371/journal.pbio.0040088.PubMed CentralView ArticlePubMedGoogle Scholar
- Drummond AJ, Rambaut A: BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol. 2007, 7: 214.36-10.1186/1471-2148-7-214.View ArticleGoogle Scholar
- Drummond AJ, Rambaut A, Shapiro B, Pybus OG: Bayesian coalescent inference of past population dynamics from molecular sequences. Mol Biol Evol. 2005, 22: 1185-1192. 10.1093/molbev/msi103.View ArticlePubMedGoogle Scholar
- Atkinson QD, Gray RD, Drummond AJ: Bayesian coalescent inference of major human mitochondrial DNA haplogroup expansions in Africa. Proc Biol Sci. 2009, 276: 367-373. 10.1098/rspb.2008.0785.PubMed CentralView ArticlePubMedGoogle Scholar
- Pereira L, Cunha C, Amorim A: Predicting sampling saturation of mtDNA haplotypes: an application to an enlarged Portuguese database. Int J Legal Med. 2004, 118: 132-136. 10.1007/s00414-003-0424-1.View ArticlePubMedGoogle Scholar
- Behar DM, Metspalu E, Kivisild T, Achilli A, Hadid Y, Tzur S, Pereira L, Amorim A, Quintana-Murci L, Majamaa K, Herrnstadt C, Howell N, Balanovsky O, Kutuev I, Pshenichnov A, Gurwitz D, Bonne-Tamir B, Torroni A, Villems R, Skorecki K: The matrilineal ancestry of Ashkenazi Jewry: portrait of a recent founder event. Am J Hum Genet. 2006, 78: 487-497. 10.1086/500307.PubMed CentralView ArticlePubMedGoogle Scholar
- Maca-Meyer N, Gonzalez AM, Larruga JM, Flores C, Cabrera VM: Major genomic mitochondrial lineages delineate early human expansions. BMC Genet. 2001, 2: 13-10.1186/1471-2156-2-13.PubMed CentralView ArticlePubMedGoogle Scholar
- Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 1999, 41: 95-98.Google Scholar
- Bandelt H-J, Achilli A, Kong QP, Salas A, Lutz-Bonengel S, Sun C, Zhang YP, Torroni A, Yao YG: Low "penetrance" of phylogenetic knowledge in mitochondrial disease studies. Biochem Biophys Res Commun. 2005, 333: 122-130. 10.1016/j.bbrc.2005.04.055.View ArticlePubMedGoogle Scholar
- Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N: Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet. 1999, 23: 147-10.1038/13779.View ArticlePubMedGoogle Scholar
- Achilli A, Rengo C, Battaglia V, Pala M, Olivieri A, Fornarino S, Magri C, Scozzari R, Babudri N, Santachiara-Benerecetti AS, Bandelt H-J, Semino O, Torroni A: Saami and Berbers - an unexpected mitochondrial DNA link. Am J Hum Genet. 2005, 76: 883-886. 10.1086/430073.PubMed CentralView ArticlePubMedGoogle Scholar
- Fraumene C, Belle EM, Castrì L, Sanna S, Mancosu G, Cosso M, Marras F, Barbujani G, Pirastu M, Angius A: High resolution analysis and phylogenetic network construction using complete mtDNA sequences in sardinian genetic isolates. Mol Biol Evol. 2006, 23: 2101-2111. 10.1093/molbev/msl084.View ArticlePubMedGoogle Scholar
- Hartmann A, Thieme M, Nanduri LK, Stempfl T, Moehle C, Kivisild T, Oefner PJ: Validation of microarray-based resequencing of 93 worldwide mitochondrial genomes. Hum Mutat. 2009, 30: 115-122. 10.1002/humu.20816.View ArticlePubMedGoogle Scholar
- Costa MD, Cherni L, Fernandes V, Freitas F, Ammar El Gaaied AB, Pereira L: Data from complete mtDNA sequencing of Tunisian centenarians: testing haplogroup association and the "golden mean" to longevity. Mech Ageing Dev. 2009, 130: 222-226. 10.1016/j.mad.2008.12.001.View ArticlePubMedGoogle Scholar
- Bandelt H-J, Forster P, Sykes BC, Richards MB: Mitochondrial portraits of human populations using median networks. Genetics. 1995, 141: 743-753.PubMed CentralPubMedGoogle Scholar
- Saillard J, Forster P, Lynnerup N, Bandelt H-J, Nørby S: mtDNA variation among Greenland Eskimos: the edge of the Beringian expansion. Am J Hum Genet. 2000, 67: 718-726. 10.1086/303038.PubMed CentralView ArticlePubMedGoogle Scholar
- Yang Z: PAML: a program package for phylogenetic analysis by maximum likelihood. Comput Appl Biosci. 1997, 13: 555-556.PubMedGoogle Scholar
- Davies W: A very model of a model human industry: New perspectives on the origins and spread of the Aurignacians in Europe. Proceedings of the Prehistoric Society. 2001, 67: 195-217.View ArticleGoogle Scholar
- Pala M, Achilli A, Olivieri A, Kashani BH, Perego UA, Sanna D, Metspalu E, Tambets K, Tamm E, Accetturo M, Carossa V, Lancioni H, Panara F, Zimmermann B, Huber G, Al-Zahery N, Brisighelli F, Woodward SR, Francalacci P, Parson W, Salas A, Behar DM, Villems R, Semino O, Bandelt H-J, Torroni A: Mitochondrial haplogroup U5b3: a distant echo of the epipaleolithic in Italy and the legacy of the early Sardinians. Am J Hum Genet. 2009, 84: 814-821. 10.1016/j.ajhg.2009.05.004.PubMed CentralView ArticlePubMedGoogle Scholar
- Malone C: The Italian Neolithic: A Synthesis of Research. Journal of World Prehistory. 2003, 17: 235-312. 10.1023/B:JOWO.0000012729.36053.42.View ArticleGoogle Scholar
- Malyarchuk B, Derenko M, Grzybowski T, Perkova M, Rogalla U, Vanecek T, Tsybovsky I: The peopling of Europe from the mitochondrial haplogroup U5 perspective. PLoS One. 2010, 5: e10285-10.1371/journal.pone.0010285.PubMed CentralView ArticlePubMedGoogle Scholar
- Bramanti B, Thomas MG, Haak W, Unterlaender M, Jores P, Tambets K, Antanaitis-Jacobs I, Haidle MN, Jankauskas R, Kind CJ, Lueth F, Terberger T, Hiller J, Matsumura S, Forster P, Burger J: Genetic discontinuity between local hunter-gatherers and central Europe's first farmers. Science. 2009, 326: 137-140. 10.1126/science.1176869.View ArticlePubMedGoogle Scholar
- Atkinson QD, Gray RD, Drummond AJ: mtDNA variation predicts population size in humans and reveals a major Southern Asian chapter in human prehistory. Mol Biol Evol. 2008, 25: 468-474. 10.1093/molbev/msm277.View ArticlePubMedGoogle Scholar
- Irish JD: The Iberomaurusian enigma: North African progenitor or dead end?. J Hum Evol. 2000, 39: 393-410. 10.1006/jhev.2000.0430.View ArticlePubMedGoogle Scholar
- Torroni A, Bandelt H-J, Macaulay V, Richards M, Cruciani F, Rengo C, Martinez-Cabrera V, Villems R, Kivisild T, Metspalu E, Parik J, Tolk HV, Tambets K, Forster P, Karger B, Francalacci P, Rudan P, Janicijevic B, Rickards O, Savontaus ML, Huoponen K, Laitinen V, Koivumäki S, Sykes B, Hickey E, Novelletto A, Moral P, Sellitto D, Coppa A, Al-Zaheri N, Santachiara-Benerecetti AS, Semino O, Scozzari R: A signal, from human mtDNA, of postglacial recolonization in Europe. Am J Hum Genet. 2001, 69: 844-852. 10.1086/323485.PubMed CentralView ArticlePubMedGoogle Scholar
- Achilli A, Rengo C, Magri C, Battaglia V, Olivieri A, Scozzari R, Cruciani F, Zeviani M, Briem E, Carelli V, Moral P, Dugoujon JM, Roostalu U, Loogväli EL, Kivisild T, Bandelt H-J, Richards M, Villems R, Santachiara-Benerecetti AS, Semino O, Torroni A: The molecular dissection of mtDNA haplogroup H confirms that the Franco-Cantabrian glacial refuge was a major source for the European gene pool. Am J Hum Genet. 2004, 75: 910-918. 10.1086/425590.PubMed CentralView ArticlePubMedGoogle Scholar
- Loogväli EL, Roostalu U, Malyarchuk BA, Derenko MV, Kivisild T, Metspalu E, Tambets K, Reidla M, Tolk HV, Parik J, Pennarun E, Laos S, Lunkina A, Golubenko M, Barac L, Pericic M, Balanovsky OP, Gusar V, Khusnutdinova EK, Stepanov V, Puzyrev V, Rudan P, Balanovska EV, Grechanina E, Richard C, Moisan JP, Chaventré A, Anagnou NP, Pappa KI, Michalodimitrakis EN, Claustres M, Gölge M, Mikerezi I, Usanga E, Villems R: Disuniting uniformity: a pied cladistic canvas of mtDNA haplogroup H in Eurasia. Mol Biol Evol. 2004, 21: 2012-2021.View ArticlePubMedGoogle Scholar
- Pereira L, Richards M, Goios A, Alonso A, Albarrán C, Garcia O, Behar DM, Gölge M, Hatina J, Al-Gazali L, Bradley DG, Macaulay V, Amorim A: High-resolution mtDNA evidence for the late-glacial resettlement of Europe from an Iberian refugium. Genome Res. 2005, 15: 19-24. 10.1101/gr.3182305.PubMed CentralView ArticlePubMedGoogle Scholar
- Ennafaa H, Cabrera VM, Abu-Amero KK, González AM, Amor MB, Bouhaha R, Dzimiri N, Elgaaïed AB, Larruga JM: Mitochondrial DNA haplogroup H structure in North Africa. BMC Genet. 2009, 10: 8-10.1186/1471-2156-10-8.PubMed CentralView ArticlePubMedGoogle Scholar
- Cherni L, Fernandes V, Pereira JB, Costa MD, Goios A, Frigi S, Yacoubi-Loueslati B, Amor MB, Slama A, Amorim A, El Gaaied AB, Pereira L: Post-last glacial maximum expansion from Iberia to North Africa revealed by fine characterization of mtDNA H haplogroup in Tunisia. Am J Phys Anthropol. 2009, 139: 253-260. 10.1002/ajpa.20979.View ArticlePubMedGoogle Scholar
- Coudray C, Olivieri A, Achilli A, Pala M, Melhaoui M, Cherkaoui M, El-Chennawi F, Kossmann M, Torroni A, Dugoujon JM: The complex and diversified mitochondrial gene pool of Berber populations. Ann Hum Genet. 2009, 73: 196-214. 10.1111/j.1469-1809.2008.00493.x.View ArticlePubMedGoogle Scholar
- Pereira L, Cerný V, Cerezo M, Silva NM, Hájek M, Vašíková A, Kujanová M, Brdička R, Salas A: Linking the sub-Saharan and West Eurasian gene pools: maternal and paternal heritage of the Tuareg nomads from the African Sahel. Eur J Hum Genet. 2010, 18: 915-923. 10.1038/ejhg.2010.21.PubMed CentralView ArticlePubMedGoogle Scholar
- Cerny V, Salas A, Hajek M, Zaloudkova M, Brdicka R: A bidirectional corridor in the Sahel-Sudan belt and the distinctive features of the Chad Basin populations: A history revealed by the mitochondrial DNA genome. Ann Hum Genet. 2007, 71: 433-452. 10.1111/j.1469-1809.2006.00339.x.View ArticlePubMedGoogle Scholar
- Harich N, Costa MD, Fernandes V, Kandil M, Pereira JB, Silva NM, Pereira L: The trans-Saharan slave trade - clues from interpolation analyses and high-resolution characterization of mitochondrial DNA lineages. BMC Evol Biol. 2010, 10: 138-10.1186/1471-2148-10-138.PubMed CentralView ArticlePubMedGoogle Scholar
- Kujanova M, Pereira L, Fernandes V, Pereira JB, Cerny V: Near Eastern Neolithic Genetic Input in a Small Oasis of the Egyptian Western Desert. Am J Phys Anthropol. 2009, 140: 336-346. 10.1002/ajpa.21078.View ArticlePubMedGoogle Scholar
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