- Research article
- Open Access
A phylogenetic estimate for golden moles (Mammalia, Afrotheria, Chrysochloridae)
BMC Evolutionary Biology volume 10, Article number: 69 (2010)
Golden moles (Chrysochloridae) are small, subterranean, afrotherian mammals from South Africa and neighboring regions. Of the 21 species now recognized, some (e.g., Chrysochloris asiatica, Amblysomus hottentotus) are relatively common, whereas others (e.g., species of Chrysospalax, Cryptochloris, Neamblysomus) are rare and endangered. Here, we use a combined analysis of partial sequences of the nuclear GHR gene and morphological characters to derive a phylogeny of species in the family Chrysochloridae.
Although not all nodes of the combined analysis have high support values, the overall pattern of relationships obtained from different methods of phylogeny reconstruction allow us to make several recommendations regarding the current taxonomy of golden moles. We elevate Huetia to generic status to include the species leucorhinus and confirm the use of the Linnean binomial Carpitalpa arendsi, which belongs within Amblysominae along with Amblysomus and Neamblysomus. A second group, Chrysochlorinae, includes Chrysochloris, Cryptochloris, Huetia, Eremitalpa, Chrysospalax, and Calcochloris. Bayesian methods make chrysochlorines paraphyletic by placing the root within them, coinciding with root positions favored by a majority of randomly-generated outgroup taxa. Maximum Parsimony (MP) places the root either between chrysochlorines and amblysomines (with Chlorotalpa as sister taxon to amblysomines), or at Chlorotalpa, with the former two groups reconstructed as monophyletic in all optimal MP trees.
The inclusion of additional genetic loci for this clade is important to confirm our taxonomic results and resolve the chrysochlorid root. Nevertheless, our optimal topologies support a division of chrysochlorids into amblysomines and chrysochlorines, with Chlorotalpa intermediate between the two. Furthermore, evolution of the chrysochlorid malleus exhibits homoplasy. The elongate malleus has evolved just once in the Cryptochloris-Chrysochloris group; other changes in shape have occurred at multiple nodes, regardless of how the root is resolved.
Golden moles (Chrysochloridae) are small, burrowing mammals endemic to sub-Saharan Africa. Bronner and Jenkins  divide the group into nine genera and 21 species, most of which are recorded from South Africa. They have been dubbed "spectacularly autapomorphic"  and are among the most unusual of mammals, showing three long bones in the forearm , hypertrophied middle ear ossicles [4–6], and a hyoid-mandible articulation , among other features. They converge in many ways on the phenotype of other subterranean mammals, such as lipotyphlan moles (Talpidae), burrowing rodents (Bathyergidae), certain armadillos (Chlamyphorus), and marsupial moles (Notoryctidae), but lack a close phylogenetic relationship with these other groups. Rather, chrysochlorids are now understood to be part of Afrotheria, a radiation of endemic African mammals, also including hyracoids (hyraxes), proboscideans (elephants), sirenians (sea cows), macroscelidids (sengis or elephant shrews), tubulidentates (aardvarks), and tenrecids (tenrecs) [8–12].
Previous taxonomic treatments of chrysochlorids have differed not only in terms of the implied intra-familial interrelationships, but also in the number of genera used to categorize a roughly similar hypodigm of extant species. In contrast to the nine genera given by Bronner and Jenkins , the number of genera recognized by other authors ranges from five to seven [13–15]. Uncertainty surrounding difference among previous taxonomic treatments are likely the result of emphasis on of different types of characters that vary in phylogenetic signal.
The only previous, character-based phylogenies of chrysochlorids were based on hyoid shape , chromosome morphology , and craniodental anatomy  from approximately a dozen species (Fig. 1A). Simonetta  also presented an estimate of chrysochlorid interrelationships (Fig. 1B), but did not base his on an explicit matrix of morphological or molecular data. These published trees contradict at least some genus-level relationships frequently implied in the literature . For example, both Simonetta  and Bronner  imply paraphyly of the genus Chrysochloris, consisting of the Cape golden mole, Chrysochloris asiatica, and a tropical congener, C. stuhlmanni (Fig. 1). The geographical disparity of Chrysochloris populations---with over 3000 km separating the Western Cape province of South Africa (C. asiatica) from the region surrounding Lake Victoria (C. stuhlmanni)---lends itself to the possibility of genus-level paraphyly. Similarly, occurrences within what previous authors  referred to as Calcochloris are separated by at least 2000 km: C. obtusirostris in southern Mozambique and "C." leucorhinus in the Democratic Republic of the Congo and Angola. Other classifications of leucorhinus (e.g., in the genus Chlorotalpa  along with C. duthieae and C. sclateri from South Africa) do not improve the geographic cohesiveness of this taxon. Hence, the monophyly of some chrysochlorid genera has not been consistently supported [13, 17] and deserves to be tested.
Here, we present a phylogenetic analysis of the Chrysochloridae based on 145 morphological characters from the cranium, dentition, and skeleton, combined with approximately 700-900 bases from exon 10 of the nuclear Growth Hormone Receptor (GHR) gene for 18 of the group's currently recognized 21 species. This locus has frequently been applied to questions regarding afrotherian systematics [e.g., [19, 20]], and along with other nuclear sequences supports a sister-group relationship between chrysochlorids and tenrecids [e.g., ]. This study attempts to provide an evolutionary basis for chrysochlorid classification and thereby aid the process of understanding chrysochlorid systematics and evolution.
The Chrysochloridae and several clades within it are recovered with high support from our Bayesian and MP analyses of the combined morphology-GHR dataset (Fig. 2), including the genera Amblysomus, Neamblysomus, Chlorotalpa, and Chrysospalax. The genus Chrysochloris, including both C. stuhlmanni from near Lake Victoria and C. asiatica from the Western Cape, is also supported as monophyletic with high Bayesian but low MP support (Fig. 2). Both MP and Bayesian analyses support the sister taxon status of Cryptochloris and Chrysochloris, again with Bayesian support higher than that from MP. In agreement with both Simonetta (reference thirteen: fig. 2) and Bronner , "Calcochloris" leucorhinus does not form a clade with Calcochloris obtusirostris in either the Bayesian or MP analyses (Fig. 2), making Calcochloris (sensu ) paraphyletic. For this reason, we regard Huetia (previously used as a subgenus ) as the appropriate generic name for the species leucorhinus.
The association of Carpitalpa arendsi with Neamblysomus (Fig. 2) is consistent with the recognition of Carpitalpa as a distinct genus , in contrast to previous classifications which assigned arendsi to either Chlorotalpa  or Amblysomus . Amblysomus and Chlorotalpa comprise successively distant sister taxa to the Carpitalpa-Neamblysomus clade following our optimal Bayesian trees; MP agrees concerning Amblysomus but does not consistently resolve the position of Chlorotalpa.
Bayesian analyses do not resolve the chrysochlorid root, but favor Eremitalpa and a Huetia-Chrysochloris-Cryptochloris group as the basal-most taxa (Fig. 2A). Results from MP also show an unresolved root, but differ by reconstructing chrysochlorines as monophyletic (Fig. 2B). In half of the optimal trees, MP places the root between monophyletic chrysochlorines and amblysomines plus Chlorotalpa, or with Chlorotalpa itself as the basal-most clade, also with a monophyletic Chrysochlorinae and Amblysominae. Despite this difference, the unrooted topologies supported by Bayesian and MP algorithms are mutually consistent (Fig. 3).
To further examine the position of the root, and to infer the node potentially most susceptible to long branch attraction , we tested the affinities of 100 randomly-generated taxa, each of which was used in turn as the outgroup for an MP analysis of our 18 chrysochlorid taxa. As depicted in Fig. 3, 36 of these outgroups rooted the chrysochlorid tree at or within Chrysospalax, 23 at Eremitalpa, 7 at or within Neamblysomus, 6 at Carpitalpa, 6 at Calcochloris, and 5 at or within Chrysochloris. Seven random outgroups yielded topologies that lacked a resolved root in a majority rule consensus of the optimal trees for that analysis. The remaining root-positions exhibited by the randomly-generated outgroups occurred at frequencies between 1-4 (Fig. 3). Hence, the equally-weighted MP analysis of GHR, morphology, and indels (Fig. 2B) does not root the chrysochlorid tree near the branches to which the greatest number of randomly generated outgroups are attracted, but the Bayesian analysis does. As previously noted, the optimal MP trees show a root at either Chlorotalpa or between chrysochlorines and amblysomines+Chlorotalpa. Only four of the 100 randomly-generated outgroups rooted the chrysochlorid tree adjacent to these nodes (Fig. 3). Bayesian analyses, in contrast, more frequently rooted the chrysochlorid topology at or near one of the longest branches within the chrysochlorid tree, albeit without strong support values.
Overall, both MP and Bayesian algorithms agree on the basic branching pattern within chrysochlorids (Fig. 3): an Amblysomus-Neamblysomus-Carpitalpa group, a Chrysospalax-Calcochloris-Chrysochloris-Cryptochloris-Huetia-Eremitalpa group, with the two species of Chlorotalpa between the two. However, we acknowledge that support indices for most basal nodes remain low, and we cannot yet be certain about the position of the chrysochlorid root.
Fig. 4 shows the trees supported by the GHR (Figs. 4A, B) and morphology (Fig. 4C) partitions examined separately. Aspects of non-chrysochlorid afrotherian phylogeny are discussed elsewhere . For chrysochlorids, the molecular and morphological partitions agree on supporting Amblysomus, Chrysospalax, Neamblysomus, and Chrysochloridae. Morphology alone does not resolve most supra-generic clades, and where resolution exists, support is weak. It does resolve a Neamblysomus-Amblysomus clade, but without Carpitalpa arendsi as portrayed in the combined (Fig. 2) and GHR (Figs. 4A, B) datasets. Morphological data weakly support an Eremitalpa-Cryptochloris clade and a Chlorotalpa-Carpitalpa-Calcochloris-Huetia-Amblysomus-Neamblysomus clade, groupings which collapse when MP is relaxed by one step. Nevertheless, as documented elsewhere [22–24], the performance of a given partition in isolation should not prevent its combination with other datasets. As shown in the distribution of Hidden Branch Support values [HBS, see Table 1 and [22–24]], for the 19 clades that are present in both the combined (Fig. 2B) and GHR-only (Fig. 4A) MP trees, 12 show increased branch support with addition of morphology and indels, 4 do not change, and 3 show decreased branch support.
As noted above, the morphological partition alone does not recover most suprageneric clades of the combined-data analysis, and weakly disagrees with many aspects of the GHR tree (Fig. 4). However, it has been demonstrated that a given clade may receive support from a combined dataset even when that clade lacks support from every partition analyzed in isolation [22, 23]. For example, at high taxonomic levels within placental mammals, morphology (among other partitions) has been shown to contribute positively to clade support in a combined analysis, whether or not that clade is present in a tree derived from morphological data alone . For the present dataset, the addition of the morphological partition improves branch support for the majority of nodes present in both the optimal MP and Bayesian topologies of the combined dataset (Fig. 2; Table 1). This observation is consistent with the observation made elsewhere [22–24], based on independent datasets, in which interaction between partitions can increase support beyond the sum of branch supports obtained from partitions in isolation (i.e., "hidden support" of ). Hence, we base our discussion on the combined-data tree.
Our results are partly congruent with the suggestion of Bronner and Jenkins  that chrysochlorids may be divided into two clades: Amblysominae and Chrysochlorinae, although their content is different according to our results. We place Amblysomus, Neamblysomus, and Carpitalpa arendsi in Amblysominae. The Chrysochlorinae of Bronner and Jenkins , excluding Carpitalpa and Chlorotalpa, is a potentially monophyletic clade (Figs. 2B, 3), and is weakly supported as such by our MP analyses of the combined data (Fig. 2B). However, additional data are necessary to address the possibility that the chrysochlorid root falls within this group, possibly near Eremitalpa (Fig. 2A). For ease of discussion, we use the terms amblysomines and chrysochlorines as defined above, but acknowledge that the latter term may be rendered paraphyletic with further phylogenetic scrutiny.
Morphological character evolution
As noted previously, chrysochlorids share a number of features (e.g., three forearm long-bones, hyoid-mandible articulation, hypertrophied malleus) seldom seen elsewhere among living mammals. In the case of ossicular morphology, considerable variation exists across chrysochlorid species [4–6, 25, 26]. Amblysomus, Neamblysomus, and Calcochloris show a relatively small malleus (Fig. 5A), unlike the elongate, club-shaped ossicle seen in Chrysochloris and Cryptochloris (Fig. 5D). The mallear head is enlarged and globular in Eremitalpa (Fig. 5E) and Chrysospalax (Fig. 5F), and is also globular but only slightly enlarged (relative to Amblysomus) in Chlorotalpa (Fig. 5B), Huetia, and Carpitalpa (Fig. 5C). Those taxa with a club-shaped malleus all possess a substantial bulge in the posterior aspect of their orbitotemporal fossa, known as the temporal bulla, which serves as a dorsal continuation of the epitympanic recess for housing the enlarged malleus (Fig. 6). The globular malleus of Eremitalpa is sufficiently large that it too results in an externally visible temporal bulla (Fig. 6C). In the other taxa with a globular malleus (Chlorotalpa, Huetia, and Carpitalpa), the ossicle is smaller and does not result in an externally distinct temporal bulla.
There are a number of other variations on chrysochlorid mallear morphology not accounted for in the current morphological matrix, such as orientation of the manubrium mallei, shared for example in Eremitalpa and Chrysochloris but not in Chrysospalax (Fig. 5; M. Mason pers. commun. and [4–6]). Here, we focus on size and shape of the mallear head, which has figured prominently in previous classifications. This region guided the classification of Simonetta , who stated " [mallear] morphology pointed to three divergent evolutionary trends, so that the family could be ... divided into three subfamilies" (p. 33 of ref. ). He further implied the intuitive view that the "normal" (i.e., small) malleus of Amblysomus is primitive, and inferred a "morphological sequence" from Amblysomus to the taxa with a globular malleus to the "extreme" club-shape observed in Chrysochloris.
Interestingly, the taxa most frequently inferred as basal by Bayesian analyses possess an enlarged, globular malleus (i.e., Eremitalpa), a slightly enlarged malleus (Huetia), or an elongate malleus (Chrysochloris and Cryptochloris). If such a taxon occupies the base of the chrysochlorid tree, then Simonetta's intuition about the character evolution of the chysochlorid malleus is wrong: taxa with an unenlarged malleus (Amblysomus, Neamblysomus, Calcochloris) occupy relatively nested branches. In contrast, the root supported by four of the eight MP analyses (Fig. 3) shows Calcochloris obtusirostris (with a small malleus) at the base of a monophyletic Chrysochlorinae, which forms the sister taxon of a Chlorotalpa-amblysomine clade. This scenario is potentially consistent with the view that a small malleus (Fig. 5A) characterized the basal-most living chrysochlorids, with enlargement occurring independently within each subfamily. Most phylogenies of Afrotheria support a tenrec-golden mole association [10, 11], and one would expect that the chrysochlorid common ancestor with other afrotherians would have had relatively small ear ossicles. Their scant fossil record [27, 28] indicates that Miocene chrysochlorids lacked a temporal bulla and therefore would not have had a mallear head of the kind seen in Chrysochloris (Fig. 5D), Eremitalpa (Fig. 5E), or Chrysospalax (Fig. 5F).
Previous discussions of mallear evolution in chrysochlorids have also noted the high probability of homoplasy in this region [4, 5, 26]. While we cannot yet resolve the position of the chrysochlorid root, all of our optimal trees agree with recent authors that mallear enlargement has not occurred in a simple progression from small, to globular, to elongate. The paraphyly of chrysochlorids with an enlarged, globular malleus indicates the presence of homoplasy in the occurrence and direction of ossicular enlargement.
Based on the phylogeny in Fig. 2, a few additional morphological characters optimize with relatively low homoplasy across chrysochlorids. The position of the foramen ovale relative to the foramen for the inferior ramus of the stapedial artery (Fig. 7) shows relatively little homoplasy in the optimal MP and Bayesian trees: the two are confluent in chrysochlorines (except Calcochloris) and distinct in amblysomines and Chlorotalpa. The position of foramen ovale relative to the sphenorbital fissure (Fig. 7) also distinguishes most members of the two groups: in amblysomines and Chlorotalpa sclateri they are separated in the ventral part of the temporal fossa, whereas in Chlorotalpa duthieae and most chrysochlorines (but not Huetia or Calcochloris) they are situated close together.
Dentally, most chrysochlorines (except for Chrysospalax) lack talonids on their lower ultimate premolar (Fig. 8), whereas these are present in amblysomines (except for Neamblysomus julianae) and Chlorotalpa. Talonids on the molars are similarly lacking in chrysochlorines (except Chrysospalax and Chrysochloris stuhlmanni), but present in amblysomines (except Neamblysomus) and Chlorotalpa. The overall number of teeth in each jaw quadrant---10 in those taxa with a full complement of three molars and 9 in taxa with just two---has also figured prominently in previous chrysochlorid classifications. However, reduction of molars is restricted to Amblysomus, Calcochloris, and is variable in Neamblysomus. As such, this feature is not diagnostic for the supra-generic clades indicated in this study.
Our data support the validity of Carpitalpa arendsi, the removal of the equatorial species leucorhinus from Calcochloris, Chlorotalpa, and Amblysomus, the integrity of most chrysochlorid genera, such as Chrysochloris including species from both equatorial Africa (C. stuhlmanni) and the Western Cape province of South Africa (C. asiatica), and the association of Cryptochloris with Chrysochloris  and of Amblysomus with the Neamblysomus-Carpitalpa clade. We amend previous chrysochlorid taxonomies by using Huetia as the appropriate genus-level designation for H. leucorhinus. Both MP and Bayesian analyses support a similar unrooted topology, with amblysomines as defined above and chrysochlorines consisting of Chrysochloris, Cryptochloris, Huetia, Chrysospalax, and Calcochloris. Chlorotalpa is favored as the sister taxon to amblysomines in Bayesian analyses; whereas in the consensus of optimal MP trees, Chlorotalpa appears in an unresolved trichotomy with monophyletic amblysomines and chrysochlorines (Fig. 2B). Bayesian analyses weakly support a root within Chrysochlorinae, near Eremitalpa or a Chrysochloris-Cryptochloris-Huetia clade. As noted above, MP yields a trichotomy at the root. The fact that an intra-chrysochlorine placement of the root in some of our analyses corresponds with the region of the tree that frequently attracts randomly generated outgroup taxa leads us to regard the position of the chrysochlorid root as still unresolved and in need of further study.
An incremental, small-to-large evolution of one of the most peculiar chrysochlorid features---the enlarged malleus---is not supported by our analysis. According to topologies supported by Bayesian techniques, chrysochlorid species with a small malleus (Amblysomus, Neamblysomus, Calcochloris) are nested, not basal, within the chrysochlorid radiation. However, optimal MP topologies cannot rule out a small malleus characterizing the basal-most chrysochlorid branch. Furthermore, and regardless of the position of the root, change in mallear size has occurred multiple times during the history of this insectivoran-grade radiation of afrotherian mammals.
We obtained new GHR sequences of 17 chrysochlorid species and appended them to GHR alignment #1 of Asher and Hofreiter [; see Additional file 1]. As detailed in Table 2, skulls from the collections of the Museum für Naturkunde Berlin (ZMB) and the Transvaal Museum Pretoria (TM) comprised source material for five of these taxa. For these specimens we followed the DNA extraction procedure described in . To control for potential sequencing artifacts, we followed  and . Additional file 2 shows individual BLAST results for GHR sequence fragments derived from museum skulls. In every case, the closest match on GenBank was to the existing chrysochlorid (Chrysospalax trevelyani AF392877 from ), with a percent similarity ranging from 91-100. In most cases the match was not identical, either to the GenBank sequence or across our samples. Given the fact that some regions of GHR exon 10 are highly conserved across mammals and the relatively short length of our amplified fragments (ca. 60-160 bp), it is not surprising that a small number match entirely. Overall, we view this result as evidence that our sequences are genuine and not influenced by contamination.
The remainder of our chrysochlorid sample derived from work undertaken at the Universities of Pretoria and Cape Town (Table 2). Methods for obtaining partial GHR sequences from these taxa are as follows: genomic DNA was extracted from frozen or ethanol-preserved tissues using standard phenol-chloroform procedures . A 767 bp fragment was amplified for 16 samples using the polymerase chain reaction (PCR, ) and primers GHR-For 5'-AGCCATTCATGGCAACTATAAATC-3', and GHR-Rev 5'-ARGGCAAGGCAGTTGCTTGAG -3' (modifications from those previously published by ). PCR reactions were done from 50 - 100 ng DNA using 1 unit of Supertherm Taq polymerase (Southern Cross Biotechnologies) in a 50 μl mixture consisting of 1× reaction buffer, 2.5 mM MgCl2 and 2 mM of each dNTP, 10 pM of each primer and ddH2O under the following cycling conditions: initial denaturation for 2 min at 94°C; 35 cycles of denaturation (30 sec) at 94°C, primer-annealing at 60 - 62°C (30 sec), primer-extension (45 sec) at 72°C; final extension of 5 min at 72°C. The resulting products were purified using isopropanol/ammonium acetate precipitation and were sequenced in both directions on an automated ABI 3100 sequencer (Applied Biosystems, Johannesburg, South Africa) using ABI PRISM Big DyeTM Terminator version 3.1 chemistry (Applied Biosystems). Nucleotide sequence alignments were constructed in ClustalX (v. 1.82 ) and subsequently translated to amino acids in MacClade (v. 3.0 ) to verify the functional reading frame. The GHR alignment of specimens amplified from both museum skulls and tissue samples comprised a matrix of up to 913 aligned nucleotides, of which 211 were parsimony informative [see Additional file 1].
Discrete morphological characters based on the matrix of Asher and Hofreiter  were collected using museum collections in Pretoria, Cape Town, King William's Town, Cambridge, London, Berlin, Stockholm, and New York. A total of 337 character states are distributed across 144 characters [see Additional file 3]. Of these characters, 45 are postcranial, 37 derive from the dentition and mandible, and 62 are from other parts of the cranium. These characters are graphically documented at http://www.morphobank.org. Indels were recorded as in  and were entered as binary characters following the GHR alignment [see Additional file 1]. Gaps within the GHR alignment were treated as missing data.
Parsimony (MP) analyses were undertaken with PAUP 4.0b10 , using heuristic searches with at least 500 random addition replicates using TBR branch swapping, multiple states treated as polymorphic, and branches collapsed unless they have at least one unambiguous optimization ("COLLAPSE = MINBRLEN"). All morphological character state changes were accorded equal weight. MP bootstrap values were calculated based on 500 pseudoreplicates of a simple addition sequence. Analysis of the partitions shown in Table 1 was conducted using MP for each partition separately, using a heuristic search as above but with 100 random addition replicates.
Bayesian analyses were implemented using MrBayes 3.1 , using the HKY+G model as recommended by the AIC in MrModeltest 2.1 . We used the default models for our morphological (i.e., Mk ) and indel (i.e., restriction site ) partitions, setting MrBayes to infer coding bias assuming that only variable characters can be observed for both ("CODING = VARIABLE"). Additional analyses assuming "CODING = ALL" for the morphological partition did not appreciably change the optimal topologies. Bayesian analyses were undertaken using four independent runs, each using a random starting tree and 1,000,000 generations with one cold and three heated chains, sampling trees every 100 generations. These reached stationarity by 10,000 generations (i.e., after the first 100 trees), at which point likelihood scores reached an asymptote and did not greatly change in value. Hence, Bayesian trees and posterior probabilities derive from a majority-rule consensus of the last 9901 trees for the first of the four independent runs, ignoring the first 100 as "burn-in". In each case, the four runs of 1,000,000 generations converged on a consistent topology. Likelihood analysis (including bootstraps) of the GHR data alone also used PAUP  and the HKY+G model as estimated by the AIC in MrModeltest , i.e., transition/transversion ratio = 2.2696, nucleotide frequencies A = 0.2805, C = 0.265, G = 0.2218, T = 0.2327, assumed proportion of invariable sites = none, distribution of rates at variable sites = gamma, shape parameter = 0.8383.
In order to investigate the part(s) of the chrysochlorid tree most attracted to a long branch, we followed a method described by Sullivan and Swofford . That is, we generated 100 artificial taxa, composed of 913 randomly picked nucleotides (consistent with the number of aligned nucleotides and base frequencies [A 28%, C 27%, G 22%, T 23%] observed in the outgroup taxon Elephantulus [similar to other potential outgroups], chosen arbitrarily among afrotherian taxa known to be close to chrysochlorids), followed by randomly generated morphological (145) and indel (8) characters, all of which were binary. Each of these randomly generated "outgroups" was successively used as the root for an MP analysis sampling our 18 chrysochlorids, preserving unchanged their GHR, morphology, and indel characters. MP searches were heuristic, as described above, but with 25 random addition replicates. The position of the root was noted in each of the 100 simulations. For those analyses that did not yield a resolved root in the strict consensus, a 50% majority rule consensus was used (13 cases). Seven out of 100 cases did not yield a resolved root using majority rule and were ignored.
Bronner GN, Jenkins P: Afrosoricida. Mammal Species of the World. Edited by: Wilson DE, Reeder DM. 2005, Baltimore: Johns Hopkins University Press, 71-81.
MacPhee RDE, Novacek MJ: Definition and relationships of Lipotyphla. Mammal Phylogeny, Placentals. Edited by: Szalay FS, Novacek MJ, McKenna MC. 1993, New York: Springer-Verlag, 2: 13-31.
Dobson GE: A Monograph of the Insectivora Part II, Potamogalidae, Chrysochloridae, Talpidae. 1883, London: John van Voorst
Mason MJ: Morphology of the middle ear of golden moles (Chrysochloridae). J Zool Lond. 2003, 260: 391-403. 10.1017/S095283690300387X.
Mason MJ: Functional Morphology of the Middle Ear in Chlorotalpa Golden Moles (Mammalia, Chrysochloridae): Predictions From Three Models. J Morph. 2004, 261: 162-174. 10.1002/jmor.10235.
Mason MJ: Massive mallei in moles: middle ear adaptations subserving seismic sensitivity. Proc Inst Acoust. 2007, 29: 69-76.
Bronner GN: Comparative hyoid morphology of nine chrysochlorid species (Mammalia: Chrysochloridae). Ann Transv Mus. 1991, 35: 295-311.
Stanhope MJ, Waddell VG, Madsen O, DeJong WW, Hedges SB, Cleven GC, Kao D, Springer MS: Molecular evidence for multiple origins of the Insectivora and for a new order of endemic African mammals. Proc Nat Acad Sci USA. 2008, 95: 9967-9972. 10.1073/pnas.95.17.9967.
Robinson TJ, Seiffert ER: Afrotherian origins and interrelationships: new views and future prospects. Curr TopicsDevel Biol. 2004, 63: 37-60. full_text.
Poux C, Madsen O, Glos J, Jong WW, Vences M: Molecular phylogeny and divergence times of Malagasy tenrecs: Influence of data partitioning and taxon sampling on dating analyses. BMC Ev Biol. 2008, 8: 102-10.1186/1471-2148-8-102.
Seiffert ER: A new estimate of afrotherian phylogeny based on simultaneous analysis of genomic, morphological, and fossil evidence. BMC Evol Biol. 2007, 7: 224-10.1186/1471-2148-7-224.
Asher RJ, Bennett N, Lehmann T: The new framework for understanding placental mammal evolution. Bioessays. 2009, 31 (8): 853-864. 10.1002/bies.200900053.
Simonetta AM: A new golden mole from Somalia with an appendix on the taxonomy of the family Chrysochloridae (Mammalia: Insectivora). Monit Zool Ital. 1968, 2: 27-55.
Petter F: Remarques sur le systématique des chrysochlorides. Mammalia. 1981, 45: 49-53. 10.1515/mamm.19188.8.131.52.
Hutterer R: Order Insectivora. Mammal species of the world. Edited by: Wilson DE, Reeder DM. 1993, Washington DC: Smithsonian Institution Press, 69-130.
Bronner GN: Cytogenetic Properties of Nine Species of Golden Moles (Insectivora: Chrysochloridae). J Mammalogy. 1995, 76 (3): 957-971. 10.2307/1382766.
Bronner GN: Systematic Revision of the Golden Mole Genera Amblysomus, Chlorotalpa, and Calcochloris (Insectivora, Chrysochloromorpha, Chrysochloridae). PhD Thesis. 1995, University of Natal, Durban
Meester JAJ, Rautenbach IL, Dippenaar NJ, Baker LM: Classification of Southern African Mammals. Transv MusMonogr. 1986, 5: 1-359.
Malia MJ, Adkins RM, Allard MW: Molecular support for Afrotheria and the polyphyly of Lipotyphla based on analyses of the growth hormone receptor gene. Mol Phylogenet Evol. 2002, 24: 91-101. 10.1016/S1055-7903(02)00219-1.
Asher RJ, Hofreiter M: Tenrec phylogeny and the noninvasive extraction of nuclear DNA. Syst Biol. 2006, 55: 181-194. 10.1080/10635150500433649.
Sullivan J, Swofford DL: Are Guinea Pigs Rodents? The Importance of Adequate Models in Molecular Phylogenetics. JMamm Ev. 1997, 4: 77-86. 10.1023/A:1027314112438.
Gatesy J, OGrady P, Baker RH: Corroboration among data sets in simultaneous analysis: hidden support for phylogenetic relationships among higher level artiodactyl taxa. Cladistics. 1999, 15: 271-313. 10.1111/j.1096-0031.1999.tb00268.x.
Gatesy J, Baker RH: Hidden likelihood support in genomic data: Can forty-five wrongs make a right?. Syst Biol. 2005, 54: 483-492. 10.1080/10635150590945368.
Lee MSY, Camens AB: Strong morphological support for the molecular evolutionary tree of placental mammals. J Evol Biol. 2009, 22: 2243-2257. 10.1111/j.1420-9101.2009.01843.x.
Forster Cooper C: On the ear region of certain of the Chrysochloridae. Philos Trans R Soc Lond B. 1928, 216: 265-283. 10.1098/rstb.1928.0005.
vonMayer A, OBrien G, Sarmiento EE: Functional and systematic implications of the ear in golden moles (Chrysochloridae). J Zool. 1995, 236: 417-430. 10.1111/j.1469-7998.1995.tb02722.x.
Broom R: Some South African Pliocene and Pleistocene mammals. Ann Transv Mus. 1948, 21: 1-38.
Asher RJ: Tenrecoidea. Cenozoic Mammals of Africa. Edited by: Werdelin L, Sanders W. Berkeley: University of California Press
Rohland N, Siedel H, Hofreiter M: Non-destructive DNA extraction method for mitochondrial DNA analyses of museum specimens. Biotechniques. 2004, 36: 814-821.
Malia MJ, Adkins RM, Allard MW: Molecular support for Afrotheria and the polyphyly of Lipotyphla based on analyses of the growth hormone receptor gene. Mol Phylogenet Evol. 2002, 24: 91-101. 10.1016/S1055-7903(02)00219-1.
Sambrook E, Fritsch F, Maniatis T: Molecular cloning. 1989, Cold Spring Harbor: Cold Spring Harbor Laboratory Press
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988, 239: 487-91. 10.1126/science.2448875.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research. 1997, 25: 4876-4882. 10.1093/nar/25.24.4876.
Maddison WP, Maddison DR: MacClade analysis of phylogeny and character evolution, version 3. 2000, Sunderland MA: Sinauer Associates
O'Leary MA, Kaufman SG: MorphoBank 2.5: Web application for morphological phylogenetics and taxonomy. 2007, [http://www.morphobank.org]
Swofford DL: PAUP version 4.0b1.0. 2002, Sunderland MA: Sinauer Associates
Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19 (12): 1572-1574. 10.1093/bioinformatics/btg180.
Nylander JA: MrModeltest v2. Program distributed by the author. 2004, Uppsala University: Evolutionary Biology Centre
Lewis PO: A likelihood approach to estimating phylogeny from discrete morphological character data. Syst Biol. 2001, 50: 913-925. 10.1080/106351501753462876.
Gilbert C, Maree S, Robinson TJ: Chromosomal evolution and distribution of telomeric repeats in golden moles (Chrysochloridae, Mammalia). Cytogenet Genome Res. 2008, 121: 110-119.
RJA and NB thank the Royal Society and South African National Research Foundation for a UK-South Africa Networking Grant that enabled completion of this paper. RJA also thanks the Deutsche Forschungsgemeinschaft (grant AS 245/2-1), the Berlin Museum für Naturkunde (Frieder Mayer, Hendrik Turni), the Naturhistoriska Riksmuseet Stockholm (Per Erikson, Olavi Grönwall), the Transvaal Museum (Teresa Kearney), the Amathole Museum (Lucas Thibedi), the South African/Iziko Museum (Denise Hamerton, Kerwin van Willingh, Margaret Avery), the American Museum of Natural History (Ross MacPhee, Eileen Westwig), the Natural History Museum London (Louise Tomsett, Paula Jenkins) and the University of Cambridge Museum of Zoology. Availability of the morphological database on http://www.morphobank.org is made possible by NSF-USA Tree of Life grant 0629860. GB (Grant number 61245), NB (Grant number 43596), and PB (Grant number 2073181) acknowledge financial support from the South African National Research Foundation. MH, MM and PC acknowledge financial support from the Max Planck Society. We thank Dr. Matt Mason for comments on the manuscript and permission to redraw images of chrysochlorid ear ossicles.
Address for correspondence: http://people.pwf.cam.ac.uk/rja58/
RJA contributed to data acquisition and wrote the paper. All authors contributed to research design; SM, GB, and MH contributed to data acquisition; GB, PB and NB obtained funding for collection of South African samples for molecular data acquisition. GB contributed to morphological data coding, analysis, and interpretation, SM and PB commented on molecular data analysis and interpretation, PB and NB contributed to earlier drafts of the manuscript with respect to data interpretation. All authors have read and approved the final manuscript.
Electronic supplementary material
Additional file 2: . BLAST results for GHR sequences obtained from museum skulls. All fragments recovered maximum similarity to GHR sequences of Chrysospalax trevelyani (AF392877 ). (DOC 85 KB)
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Asher, R.J., Maree, S., Bronner, G. et al. A phylogenetic estimate for golden moles (Mammalia, Afrotheria, Chrysochloridae). BMC Evol Biol 10, 69 (2010). https://doi.org/10.1186/1471-2148-10-69
- Optimal Topology
- Maximum Parsimony
- Growth Hormone Receptor
- Sister Taxon
- Maximum Parsimony Analysis