The identity, distribution, and impacts of non-native apple snails in the continental United States
© Rawlings et al; licensee BioMed Central Ltd. 2007
Received: 03 January 2007
Accepted: 26 June 2007
Published: 26 June 2007
Since the mid 1990s populations of non-native apple snails (Ampullariidae) have been discovered with increasing frequency in the continental United States. Given the dramatic effects that introduced apple snails have had on both natural habitats and agricultural areas in Southeast Asia, their introduction to the mainland U.S. is cause for concern. We combine phylogenetic analyses of mtDNA sequences with examination of introduced populations and museum collections to clarify the identities, introduced distributions, geographical origins, and introduction histories of apple snails.
Based on sampling to date, we conclude there are five species of non-native apple snails in the continental U.S. Most significantly, we recognize three species within what has been called the channeled apple snail: Pomacea canaliculata (California and Arizona), Pomacea insularum, (Florida, Texas, and Georgia) and Pomacea haustrum (Florida). The first established populations of P. haustrum were discovered in the late 1970s in Palm Beach County Florida, and have not spread appreciably in 30 years. In contrast, populations of P. insularum were established in Texas by 1989, in Florida by the mid to late 1990s, and in Georgia by 2005, and this species continues to spread rapidly. Most introduced P. insularum haplotypes are a close match to haplotypes from the Río Uruguay near Buenos Aires, indicating cold tolerance, with the potential to spread from Florida, Georgia, and Texas through Louisiana, Alabama, Mississippi, and South Carolina. Pomacea canaliculata populations were first discovered in California in 1997. Haplotypes of introduced P. canaliculata match native-range haplotypes from near Buenos Aires, Argentina, also indicating cold tolerance and the potential to establish farther north.
The term "channeled apple snail" is descriptive of a morphology found in many apple snail species. It does not identify a single species or a monophyletic group. Clarifying species identifications permits a more accurate assessment of introduction histories and distributions, and provides a very different picture of the tempo and pattern of invasions than was inferred when the three species with channeled sutures were considered one. Matching introduced and native-range haplotypes suggests the potential for range expansion, with implications for native aquatic ecosystems and species, agriculture, and human health.
Members of the freshwater gastropod family Ampullariidae, known as apple snails, have an impressive track record as invasive species. Species in three genera, Pila, Pomacea, and Marisa, have demonstrated a tenacious ability to survive and spread rapidly in the freshwater habitats into which they have been introduced [e.g. [1–3]]. Of these, members of the genus Pomacea, which is native to South and Central America, parts of the Caribbean, and the southeastern U.S., have become widely established in many areas within Southeast Asia, Sri Lanka, Guam, Hawaii, Papua New Guinea, the Dominican Republic, parts of the mainland U.S., and possibly Australia [4–6]. Many of these introductions have resulted from the escape or release of snails from plant or animal aquaculture operations [e.g. ]. Other introductions have probably resulted from release of snails acquired through the pet trade [e.g. ], a common introduction pathway for alien species .
Within the continental U.S., there is only one native apple snail, Pomacea paludosa. This species is widely distributed across the wetlands of the Florida peninsula and extends into Florida's panhandle as far west as the Choctawhatchee River, and into warm springs in Georgia . A population also exists in Alabama, where it was introduced in 1953 . Over the past 50 years, however, Marisa cornuarietis and several species of Pomacea have invaded wetlands and waterways of the mainland U.S., and established populations of these non-native ampullariids are now present in six states: Alabama, Arizona, California, Florida, Georgia, and Texas . Since their initial appearance in the 1950s, the introductions of non-native apple snails largely occurred unimpeded and without much apparent concern [but see ]. This changed dramatically in the 1990s, however, with the appearance and rapid spread of channeled apple snails, thought to be Pomacea canaliculata, in rice-growing areas of Texas.
Channeled apple snails represent a major risk to native wetland ecosystems and agriculture. The name "channeled apple snail" was first coined as an Anglicization of the scientific name of the presumed single species Pomacea canaliculata, with reference to the distinct sutural channel between adjacent shell whorls. Nevertheless, this is a feature of many species of Pomacea, including Pomacea insularum, and Pomacea haustrum, and it has not been clear whether channeled apple snails are a single species or even a monophyletic group [10, 12]. In Southeast Asia, Pomacea canaliculata and Pomacea insularum have become devastating agricultural pests, especially of rice [4–6], raising concern for U.S. rice growing regions. In addition, these species have the potential to alter native freshwater habitats significantly, causing shifts in ecosystem state and function . In response to these concerns, the U.S. Department of Agriculture began requiring permits for importation or interstate movement of aquatic snails in 2006, specifically targeting apple snails with the hope of limiting their spread in the U.S.
Despite mounting concern, the identity of non-native ampullariids in the continental U.S., their provenance, current distributions, and means of introduction and spread remain poorly understood. Difficulty in species identification is largely a consequence of the overall conservative external morphology of the group combined with phenotypic plasticity of Pomacea species. Few rigorous morphometric studies have been carried out on the family [14–16], and the degree of overlap and variability in shell morphology makes delimitation of many species based on shell shape unreliable. This difficulty is complicated further by the fact that the aquaculture industry and pet trade have not been especially concerned with the species-level taxonomy of the snails introduced [e.g. ].
The first known ampullariid introduction into the continental U.S. was of Marisa cornuarietis, discovered in South Florida in 1957  and probably released through the pet trade. The introduction history of other ampullariids is less clear. Pomacea bridgesii was first reported in Florida by Clench in 1966 . In 1978, snails identified as Pomacea canaliculata were collected from Palm Beach County, Florida . Since then, apple snails with channeled sutures have been reported in other Florida counties, and in Alabama, Arizona, California, Georgia, and Texas, with many of these reports since about 2000.
A critical first step in management and control of alien ampullariids is to ascertain what species are present, their distributions, where they came from, and when the populations became established. By "established" we mean the presence of self-sustaining populations in natural bodies of water or man-made canals and lakes, excluding aquaculture facilities and other locations where the snails may have been maintained in artificial environments. Correct identification of species and their origins permits access to published information on their biology and ecology in their native habitats, which could be useful in understanding the tolerances, potential non-native ranges, and possible impacts as invasive species. Determining the timing of establishment helps us to understand the epidemiology of the invasion, that is, the rate and dynamics of the invasion process. Here, we use molecular phylogenetic analyses to clarify the identities, origins, and distributions of Pomacea species within the continental U.S. We supplement this with information from museum collections and illustrate shells and egg masses of each species. We then attempt to determine the time and place of initial establishment of each species, and discuss potential impacts to native ecosystems, agriculture, and human health.
Results and Discussion
Species-Level Phylogenetic Analysis
Discrete P. haustrum and P. diffusa clades formed a clade that was sister to the P. insularum, P. canaliculata, and P. paludosa clade. The position of Pomacea camena was not as robustly supported and varied among gene regions analyzed. Marisa cornuarietis haplotypes formed a well-supported monophyletic clade that was sister to a clade of Marisa planogyra and Asolene spixii, indicating that Marisa is not monophyletic.
Of the three apple snail species with channeled sutures in the continental U.S., Pomacea canaliculata occurred only in California and Arizona, P. insularum occurred in Texas, Georgia, and Florida, and P. haustrum was restricted to Florida.
Our results indicated that: 1) South American Pomacea were not monophyletic and 2) apple snails with channeled sutures were not monophyletic. This latter result is perhaps not surprising. The shells of many Pomacea species are similar in overall shape; unadorned spirally coiled tubes with overlapping whorls that do not depart appreciably from the axis of coiling. Given these general constraints, simple changes in the rate of shell whorl expansion and translation down the axis of coiling will affect the degree of overlap of shell whorls, and consequently the degree of channelling of the suture. The degree of channeling may also be affected by changes in the cross-sectional shape of the whorl. Convergence in channeled shells is thus not unexpected among the fifty or so species within the genus Pomacea.
Taxonomy, Source Populations, and Introduced Distributions
Our results indicate that five species of non-native ampullariids have been introduced to the continental U.S. Below, we assess their probable geographic origins, the timing of their initial introductions, their spread, and current distribution. We conclude with an assessment of the risks to environments, native species, agriculture, and human health that these species pose.
Pomacea haustrum (Reeve, 1856)
It is difficult to identify even the general region of the type locality for this species. The type locality was given as the Río Marañón, Brazil , but this river is in Peru, joining with the Rio Ucayali above Iquitos to become the Rio Solimões, which in turn joins with the Rio Negro to become the Amazon River of Brazil. Some of the first Europeans to explore the region in the sixteenth century, however, referred to the Amazon River as El Río Marañón, and some nineteenth century maps refer to the entire Amazon as the Marañón . It is likely that the Río Marañón of one collector was not the same as the Río Marañón of another.
The only established populations of P. haustrum known by us in the U.S. are in Palm Beach County, Florida. As of June, 2006, adults and egg masses were present in the Loxahatchee National Wildlife Refuge and in canals and lakes of some surrounding communities (Collins and Rawlings, pers. obs.).
Pomacea haustrum was reported as established in Palm Beach County after 1989 , although a specimen of P. haustrum was collected there as early as 1983 . We believe, however, that specimens collected in the 1970s and 1980s attributed to P. canaliculata (see below) are P. haustrum. For example, the earliest Florida specimen identified as P. canaliculata in the Florida Museum of Natural History (FLMNH 184660) was collected in 1978 from Palm Beach County and corresponds closely to the possible syntype of P. haustrum (Fig. 4). Also, an egg mass attributed to P. canaliculata (FLMNH 142245, collected in 1989) has traces of green color, the irregular honeycombed appearance, and the egg size of P. haustrum. Therefore, P. haustrum was probably introduced and successfully established by the late 1970s, but has failed to expand its range appreciably since then.
Pomacea canaliculata (Lamarck, 1822)
The apple snails with channeled sutures introduced into the continental U.S. have been identified as Pomacea canaliculata [e.g. [20, 24]] or referred to as part of the Pomacea canaliculata complex or group . Cowie and Thiengo  listed the native distribution of Pomacea canaliculata as Argentina, Bolivia, Paraguay, Uruguay, and Brazil. Nevertheless, Cazzaniga  suggested that all canaliculata-like ampullariids may constitute a single, very variable species, distributed throughout most of tropical and warm-temperate South America. The difficulties in discriminating Pomacea canaliculata and similar species, particularly Pomacea insularum (see below), have been noted [26, 27] and reflect not only this poor understanding of its native range but also its considerable intraspecific morphological variability [15, 28, 29]. The type locality of P. canaliculata (rivières de la Guadeloupe) is ambiguous, but may refer to Lago Guadeloupe, Argentina .
In the continental U.S., P. canaliculata is found only in Arizona and California, although we have not sampled the Alabama population of apple snails with channeled sutures. The East and West coast populations of apple snails with channeled sutures are therefore different species, and need to be treated as distinct management units. The match of haplotypes to Hawaiian samples suggests a possible pathway for its introduction to the western U.S., via the food trade.
Pomacea canaliculata was reported in Florida and Texas in the late 1970s, probably based on misidentification of P. haustrum in Florida and P. insularum in Texas. The earliest report of an established population of P. canaliculata in California is 1997 in Lake Miramar, north of San Diego . The history of other populations in California and Arizona was reviewed recently .
Pomacea insularum (d'Orbigny, 1835)
The difficulties of discriminating Pomacea insularum from P. canaliculata have been reviewed above. Pomacea insularum is reported from Argentina, Brazil , and Bolivia  and it probably occurs in Uruguay and Paraguay.
The type locality is the Río Paraná, which joins the Río Uruguay just above Buenos Aires, forming the Río de la Plata. The area between the Paraná and the Uruguay is the Argentine province of Entre Ríos, the southern part of which is marshy, with channels connecting the Paraná and the Uruguay. We sampled a specimen of P. insularum (FLMNH 159738) from Concepción del Uruguay on the Uruguay, which is closely linked to the type locality. We amplified and sequenced ~1,900 nucleotides, which corresponded closely to sequences from several populations of introduced P. insularum, differing by only two nucleotides (0.1%).
Pomacea insularum has not been reported previously in the continental U.S. Specimens identified as P. canaliculata, collected in 1978 in Palm Beach County, are P. haustrum (above). When, then, did P. insularum become established in Florida? Winner  reported ampullariids with pink egg masses in Florida fish farms in 1991. Specimens collected in Hillsborough (FLMNH 261647) and Collier Counties (FLMNH 254749) in Florida in 1996 may be P. insularum. At present, the earliest genetically confirmed specimen that is from the location of an extant population was collected in 2002 in Lake Munson (FLMNH 298514). Many populations were discovered in Florida from 2004 to 2006. The establishment of P. insularum, misidentified as P. canaliculata, may be considerably more recent than has heretofore been thought, and its spread may therefore be much more rapid than has been appreciated.
Howells et al.  reviewed the distribution history of P. insularum (as "Pomacea canaliculata-group") in Texas. The first established population (identified as P. canaliculata) was discovered in 1989 in Houston (Harris County) . Populations have since been documented in the surrounding counties of Brazoria, Galveston, Waller, Fort Bend, and Chambers .
In Georgia, P. insularum was first collected from the Alabaha River, in Blackshear (Pierce County) in February 2005. Other populations, some established, have since been discovered in ponds and streams of the Alabaha River, on St. Simon's Island (Glynn County) and at the mouth of the St. Marys River (Camden County) (Brett Albanese, pers. comm.).
Pomacea diffusa Blume, 1957
Pomacea diffusa is known as the spike-topped apple snail, because of its relatively raised spire. It lacks a channeled suture, and overlaps in size with the native P. paludosa (Fig. 2a) . The egg masses have an irregular honeycombed appearance, like those of P. haustrum, but are smaller and have a tan to salmon color (Fig. 3b), although the egg masses are white when freshly laid. Pomacea diffusa was originally described as a subspecies of Pomacea bridgesii. Pain  argued that P. bridgesii bridgesii was a larger form with a restricted range, with the smaller P. bridgesii diffusa being the common form throughout the Amazon Basin (Brazil, Peru, Bolivia). Cowie and Thiengo  suggested that the latter might deserve full species status, and the two taxa have been confirmed as distinct species by genetic analyses [, K.A. Hayes, R.C. Joshi, S.C. Thiengo and R.H. Cowie, in prep.].
The type locality of Pomacea diffusa is in the city of Santa Cruz, Bolivia, although the species is widespread throughout the Amazon Basin. Three samples from Belém, Brazil, formed a well-supported clade with non-native populations from Florida and Cuba (Fig. 5), exhibiting 0.4 to 1.8% sequence difference from the introduced populations.
Thompson  recorded this species (as P. bridgesii) in Florida in Monroe, Miami-Dade, Broward, Palm Beach, and Pinellas Counties. The FLMNH electronic database also lists samples from Alachua County, but records cited from the FLMNH database for Brevard County [e.g. ]) are in fact from Broward County. We have also collected this species in Hillsborough and Collier Counties.
Pomacea diffusa was first recorded in Florida (as P. bridgesii) by Clench . The FLMNH has specimens collected in Palm Beach County in 1967 (FLMNH 20295) and Miami-Dade and Broward Counties in the early 1970s (FLMNH 22175, 222247). Howells et al.  reported its establishment in Mobile, Alabama in 2003.
Marisa cornuarietis (Linnaeus, 1758)
Marisa cornuarietis was first discovered in Coral Gables, Florida, in 1957 . It has spread to many other counties in southern Florida [, Rawlings and Collins, pers. obs.]. It was first found in Texas in 1983  and has also been reported in California and Idaho . Its potential ecological impacts have been reviewed recently .
Potential Impacts of Non-native Pomacea
Pomacea haustrum is currently of relatively minor concern in the U.S., given its failure to spread beyond Palm Beach County after 30 years or more in Florida. It should be noted, however, that many species have maintained limited distributions, sometimes for decades, before becoming invasive . Pomacea diffusa has generally been assumed also to pose little threat in the U.S. and it is the only apple snail for which interstate transport is permitted. This lack of concern may be unwarranted. The U.S. Department of Agriculture considered it (as P. bridgesii) to be innocuous , presumably based on a study that concluded that it feeds primarily on aufwuchs, not macrophytes . The potential effects of Pomacea diffusa in natural habitats are unknown, but a conflicting study reports that, in addition to macrophytes, it will feed readily on animal carcasses, live worms, and the eggs of planorbid snails . It may therefore have direct effects on both aquatic vegetation and native snails and compete for food with native scavengers such as crayfish, shrimp, and fish.
Pomacea insularum and P. canaliculata pose the greatest threat to agriculture and native wetland ecosystems in the U.S. One of the better predictors of the effect of an invasive species is the effect of the species or related species in other areas where it has been introduced . The potential of P. canaliculata has been clearly demonstrated in Southeast Asia where its introduction into a tropical wetland ecosystem in Thailand resulted in dramatic changes in biodiversity and ecosystem functioning [13, 41, 42], and by its devastating effects on agriculture, especially wetland rice production [4–6]. Currently, the occurrence of P. canaliculata has been confirmed in California and Arizona in the continental U.S. However, it has the potential to spread into other areas, including the rice-growing parts of California, where it could cause serious damage.
Some of the ecological and agricultural impacts in Asia associated with P. canaliculata are almost certainly attributable to Pomacea insularum. This species is also widespread in the region [[27, 43], K.A. Hayes, R.C. Joshi, S.C. Thiengo, and R.H. Cowie, in prep.; see below], but has not been explicitly acknowledged as a serious pest because of the confusion in identification of these two species, with most of the literature referring to P. canaliculata. Pomacea insularum may therefore be likely to have a significant impact on aquatic ecosystems and pose a threat to crops in the southeastern U.S. , particularly given the potential for it to spread through parts of Alabama, Mississippi, Louisiana and Texas.
The match of introduced haplotypes of P. canaliculata and P. insularum to native Argentinean samples from approximately 35°S suggests that the introduced populations of these species may be cold tolerant and capable of surviving occasional frosts. Moreover, P. canaliculata occurs as far as 38–39°S, and topography rather than climate may set the natural southern limit of this species [44, 45]. The average minimum monthly temperature in Buenos Aires is 4–6 degrees Celsius (39–43 degrees Fahrenheit) from May to September, slightly lower than the average minimum winter monthly temperatures in Charleston, South Carolina. Consequently, P. insularum could potentially spread at least this far north on the Atlantic Coastal Plain, and through parts of Alabama, Mississippi, Louisiana, and Texas on the Gulf Coastal Plain. Similarly, P. canaliculata may be able to spread from its current introduced locations in California at least as far north as San Francisco. Ecological niche modeling based on the native and introduced range of these species [e.g. ] will permit a more refined estimate of potential introduced ranges. An important caveat here is that reports of the native ranges of these two species may be commingled.
In addition to collateral damage from control of non-native species, "extinction by hybridization"  could result from hybridization of P. paludosa with non-native species, which seems possible given the close phylogenetic relationship of P. insularum and P. paludosa. There is also the possibility of competitive interactions between native and non-native snails . Introduced Pomacea spp. have been reported to consume the eggs of both congenerics and other snails and invertebrates [39, 50, 51]. We have anecdotally noted a decline in abundance or the apparent disappearance of P. paludosa following introduction of P. insularum (Collins and Rawlings, pers. obs.). Pomacea paludosa is the primary food of the endangered Everglade Snail Kite , and an important food for other birds, including the limpkin, as well as fish, turtles, and alligators. At Lake Tohopekaliga (Collins and Rawlings, pers. obs.), limpkins are routinely taking P. insularum for food.
Finally, introduction of all non-native ampullariids is a concern because of the suite of associated parasites and their potential effects on apple snail predators and humans. We know nothing at present about the parasites of introduced ampullariids in the continental U.S. Ampullariids, including Pomacea canaliculata, are intermediate hosts of important vertebrate parasites , most notably nematodes in the genus Parastrongylus (=Angiostrongylus). Parastrongylus cantonensis causes eosinophilic meningoencephalitis , and P. costaricensis causes abdominal angiostrongyliasis in humans . Transmission of Parastrongylus infections to mammalian hosts requires development to the L3 larval stage in an intermediate gastropod host. The spread of Pomacea species may therefore facilitate the spread of Parastrongylus species by completing the life cycle required to infect mammalian hosts. In Beijing, China, consumption of snails identified as Pomacea canaliculata resulted in 131 cases of human Parastrongylus cantonensis infection during a 4-month period . Parastrongylus cantonensis and/or P. costaricensis are established in Florida and Louisiana [55, 57–59], so the potential interaction of Pomacea insularum with Parastrongylus species is a concern.
Clarification of species identifications, introduced ranges, and native ranges provides a more accurate picture of the tempo of apple snail invasions in the continental U.S., and suggests considerable potential for future range expansion. The situation is dynamic: during the time in which this paper was written, a new population of apple snails with channeled sutures has been discovered in New Orleans (S. Longman, pers. comm.), and preliminary genetic data (T. Collins and J. Bernatis, unpublished data) suggest that a population of P. canaliculata is present in north Florida. Considerable work remains to be done. We have sampled only about one quarter of the Florida populations of apple snails with channeled sutures, and the ranges of introduced ampullariids remain incompletely known. Other species may yet be discovered in introduced populations. At present, a combination of genetic data, morphological characters, and egg mass morphology is the best strategy for definitive identification. Finally, our genetic results are based on a single marker, although preliminary evidence from the nuclear histone 3 gene gives similar results [Hayes et al., in prep]. Higher resolution markers such as microsatellites may be useful in indicating the pathways by which non-native snails are spreading rapidly through parts of the U.S.
Sampling, DNA extraction, and sequencing
We sampled native Pomacea paludosa in Florida and non-native apple snails in Florida, Georgia, Texas, California, Arizona, Hawaii, Cuba, and Guadeloupe in the Lesser Antilles [see Additional file 1]. These included taxa identified as Pomacea canaliculata or Pomacea canaliculata-group, Pomacea bridgesii, Pomacea haustrum, Marisa cornuarietis, Pila conica and Pila polita. We also sampled native-range populations of Pomacea canaliculata-group snails, Pomacea haustrum, Pomacea diffusa, Pomacea camena, Asolene spixii, Marisa planogyra, and Pila polita. Pila conica was used as an outgroup for phylogenetic analyses [60, 61]. Specimens were also obtained from the Bishop Museum (Honolulu, BPBM), Field Museum of Natural History (Chicago, FMNH), Florida Museum of Natural History (Gainesville, FLMNH), the Muséum National d'Histoire Naturelle (Paris, MNHN), and the Natural History Museum (London, BMNH) for both molecular and morphological comparisons.
DNA was isolated from ~50 mg of tissue using standard phenol/chloroform methods , or Qiagen's Dneasy extraction kit. The polymerase chain reaction (PCR) was used to amplify two portions of the mitochondrial genome. The first consisted of ~1,900 nucleotides spanning the 3'end of the 12S rRNA, the intervening tRNA valine and the 5'end of the 16S rRNA. This region was amplified in two overlapping portions using the primers 12Sai [5'-AAACTAGGATTAGATACCCTATTAT-3'; 63] with 16Srgast [5'-GCCATGATGCAAAAGGTAC-3'], and 12Sf [5'-GCACACATCGCCCGTCGCTCT-3'; reverse complement of 12Sb' in 63] with 16SbrH-alt [5'-CCGGTCTGAACTCAGATCATGT-3'; slight modification of 16Sbr in 63]. The second region was a ~658 nucleotide portion of the cytochrome c oxidase subunit I (COI) gene amplified using slightly degenerate versions of standard primers . Reactions were performed in a MJ Research PTC-200 thermal cycler in 50 μl volumes with 1.5 mM MgCl2, each dNTP at 200 micromolar, 10–100 nanograms of genomic DNA, and 1X Promega buffer B, and 1 U of Taq polymerase (Promega, Madison, Wisconsin). PCR products were purified with a GeneClean III Kit (Bio 101, Carlsbad, California), and cycle-sequenced with Big Dye version 3.1 chemistry following the manufacturer's protocol (PE-ABI). Sequencing reactions were analyzed on an ABI Prism 3100 Genetic Analyzer. All samples were sequenced on both strands and sequences have been deposited in GenBank: rRNAs [GenBank: EF519073–EF519181, AY449500] and COI [GenBank: EF514942–EF515081].
COI sequences were generated for 141 snails. The 12S-16S region was sequenced for representatives of each unique COI haplotype and locality combination, for a data set of 108 individuals. For some specimens, primarily museum specimens, amplification did not work for all gene regions, and different gene regions failed to amplify in different snails, resulting in snails with no sequence in common. This resulted in significant degradation of support values. We therefore selected the 95 individuals with both gene regions completely sequenced, representing 46 unique haplotypes, for the among-species phylogenetic analysis. Nevertheless, some individuals with a great deal of missing data were important for matching introduced haplotypes to native ranges, and were therefore used for within-species analyses and haplotype networks.
Alignment and phylogenetic analyses
COI sequences were aligned at the amino-acid level and back aligned to nucleotides without indels in MacClade . The rRNA sequences were aligned using T-Coffee  on the IGS server with default settings . We tested alignment sensitivity by repeating phylogenetic analyses on a data set in which ambiguously aligned regions were removed by excluding positions in each direction until we came to nucleotides that were invariant in all taxa in the analysis .
We used maximum parsimony and Bayesian methods for phylogenetic analyses. Maximum parsimony bootstrap analyses were performed using PAUP* 4.0b10  with equal weighting, uninformative characters excluded, and gaps treated as missing data. Three hundred bootstrap replicates with 10 random sequence additions per replicate (RSAs) and TBR branch swapping were performed using the heuristic search option. We used the Akaike Information Criterion (AIC) to select the most appropriate model of sequence evolution using Modeltest 3.07 . Results indicated that the 12S-16S sequence and the intervening tRNA sequence could be combined in one partition. The COI sequence was divided into 3 partitions by codon position. The parameters determined by Modeltest were used to specify the models of sequence evolution, and as priors for the Bayesian analyses, which were performed using MRBAYES 3.1.2 . The prior distributions for the Bayesian analyses were as follows: topology uniform; branch lengths exponential with parameter = 10.0; gamma shape parameter exponential with parameter = 5; proportion invariant sites uniform over the interval (0, 1); and substitution rate matrix and base frequencies Dirichlet with starting values from Modeltest. We employed 4 Markov chains for 3–6 million generations and the partition-specific models described. Trees were sampled every 100th generation, discarding the first 33% of sampled trees as burn-in. We initiated two simultaneous runs from different random starting trees, and convergence was inferred when the average standard deviation of split frequencies approached 0.01, and the potential scale reduction factor for all parameters approached 1.0.
To explore further the relationships among introduced haplotypes of P. insularum from Florida, Texas, Georgia, and native-range populations, we performed a statistical parsimony analysis using TCS [version 1.20, 72]. We included 48 individuals based on the results of phylogenetic analyses (below). Some museum specimens did not amplify for all regions, resulting in significant missing data for a few individuals. Because missing data may cause problems related to calculated distances and collapsing of haplotypes in the program TCS, we eliminated characters with missing data or individuals with excessive missing data, and performed the TCS analysis based on 1781 nucleotides for which there are no missing data, with a parsimony limit of 90%.
We thank Tylan Dean, Cindy Schulz, the USFWS and the USDA for financial support, and Monica Fernandez, Aline Mattos, and especially Silvana Thiengo for assistance in Brazil. We thank Rüdiger Bieler, Phil Darby, Bill Loftus, Fred Thompson, and two anonymous reviewers for comments on the manuscript. We thank Matt Abbott, Brett Albanese, David Britton, Mike Bunting, Lyubov Burlakova, Alfredo Castro-Vazquez, Néstor Cazzaniga, Duane Choquette, Dana Denson, Lewis Funk, Stijn Ghesquiere, Michael Hall, Sandi Hanlon-Breuer, Matt Hinman, Bob Howells, William Jacobson, Penny Levin, Bill Loftus, Yvette Ogle, Mike Patterson, Jean-Pierre Pointier, Chad Sexton, Paul Sharp, Adam Silagi, Brian Taylor, Jess Van Dyke, James Walker, and Mo Way for providing samples. We also thank the following museum staff for assistance with accessing material: Philippe Bouchet and Virginie Héros (Muséum National d'Histoire Naturelle), Jochen Gerber (Field Museum), Regina Kawamoto (Bishop Museum), Peter Mordan (The Natural History Museum), and John Slapcinsky (Florida Museum of Natural History).
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