Research article | Open | Published:
Effect of temperature and glia in brain size enlargement and origin of allometric body-brain size scaling in vertebrates
BMC Evolutionary Biologyvolume 14, Article number: 178 (2014)
Brain signaling requires energy. The cost of maintaining and supporting energetically demanding neurons is the key constraint on brain size. The dramatic increase in brain size among mammals and birds cannot be understood without solving this conundrum: larger brains, with more neurons, consume more energy.
Here we examined the intrinsic relationships between metabolism, body-brain size ratios and neuronal densities of both endothermic and ectothermic animals. We formulated a general model to elucidate the key factors that correlate with brain enlargement, and the origin of allometric body-brain size scaling. This framework identified temperature as a critical factor in brain enlargement via temperature-regulated changes in metabolism. Our framework predicts that ectothermic animals living in tropical climates should have brain sizes that are several times larger than those of ectothermic animals living in cold climates. This prediction was confirmed by data from experiments in fish brains. Our framework also suggests that a rapid increase in the number of less energy-demanding glial cells may be another important factor contributing to the ten-fold increase in the brain sizes of endotherms compared with ectotherms.
This study thus provides a quantitative theory that predicts the brain sizes of all the major types of animals and quantifies the contributions of temperature-dependent metabolism, body size and neuronal density.
Brain size is a traditional metric of intelligence. Species with larger brain-body size ratios are often associated with greater intelligence, better tool-making skills, more social interactions, and other enhanced characteristics . Although many correlations between large brains and complex behavioral measures have been posited over the past century, the driving forces and key factors underlying the development of larger brain-body size ratios in mammals and birds are still an open issue -. The evolution of large brains in mammals and birds coincided with the development of endothermy  at the end of the Triassic period (~200 million years ago)  during which time the first mammals evolved from therapsids (i.e., the mammal-like reptiles) and has continued in primates and humans, which have the largest brain-to-body weight ratios , to the present day . In 1891, Snell  first described the allometric scaling relationship that emphasizes the dependence of brain size on body size. The most striking observation is that mammals and birds have developed larger brains that are approximately 5–50 times heavier than those of the lower-level vertebrates (e.g., reptiles, fish and amphibians) with similar body mass. It is surprising that mammals and birds developed larger brains, because, brain tissue is extremely demanding in terms of metabolic consumption, because the neuronal electronic signaling that occurs within the brain may have a per-neuron energy cost as high as ten times that of other cells in the body. This difference has attracted much attention to the search for links between brain size, body size and metabolic production. Martin  proposed that brain size is linked to maternal metabolic turnover via a 3/4 allometric scaling law . This work indicated that the total amount of energy available from the body to supply the brain may constrain the size of the brain; however, species with larger brains do not have basal metabolic rates significantly higher than those in species with smaller brains ,,-. There is also no significant difference in the percentage of energy supplied by the body to the brain between endotherms and ectotherms ,. Given these similar metabolic processes and energy supply ratios, why do mammals and birds have larger brains than fish and reptiles of similar weight? How do mammals and birds meet the energy demands of brains that are 5–50 times larger? Although much research has focused on the several-fold differences in the sizes of the brains of primates and those of low-level mammals by considering the improved energy source acquisition styles of primates, such as improvements in food hunting style due to the use of tools, the consumption of cooked food, and differences in metabolic allocation, few studies have focused on the proximate causes of the differences in brain size between lower-level animals (e.g., fish, reptiles and amphibians) and higher-level animals (e.g., primates, mammals and birds). What is the role of temperature and metabolism in promoting or constraining brain size? What is the relative contribution of neuron and glial densities in increasing brain size?
In the present study, we re-examined the relationships between temperature, metabolism, neuronal density, energy cost of individual neurons, brain size and body size across of all the major types of vertebrates. Here we specifically focus on the critical effect of temperature and growth of glia cells in the development of large brain-body size ratio in mammals and birds and origin of allometric body-brain size scaling in vertebrates. We then develop a general model to describe the intrinsic relationship among the examined factors.
Body temperature, metabolism, external temperature and brain size
We first re-plotted the relationships between brain size and body size across different species (Figure 1A and Additional file 1: Table S1). In each type of species, the relationship between brain size and body size is highly ordered. Brain size increases with body size, and the relationship can be fit using the following allometric equation ,:
where exponent α is the allometric slope, C is a constant, and W and P are brain and body weight, respectively. Analyses have shown that C = 0.078 and α = 0.689 for endotherms (including mammals, birds, insectivores, primates, dolphins and humans), and C = 0.014 and α = 0.578 for ectotherms (primarily fish, reptiles and amphibians). The parameters C and α vary across species (see Additional file 1: Table S1). A closer examination of the distributions of body mass versus brain mass reveals that the warm-blooded, endothermic animals with relatively constant body temperatures of approximately 8°C (e.g., mammals, birds, primates and dolphins) form one distinct distribution, and cold-blooded ectothermic animals with body temperatures that vary with the environment and average approximately 16°C (i.e., fish, reptiles and amphibians) form a clear second distribution. Warm-blooded species have brains that are 4–43 times larger than those of ectothermic animals. The fact that the most apparent difference between the two groups is temperature suggests that it is a key factor that correlates with brain size.
If temperature is important, we might predict that cold-blooded species living in disparate environmental temperatures might also have different brain sizes. To examine whether the extrinsic, i.e., environmental, temperature also explains the variation in brain size, we used fish brain and body-size data from locations of different temperatures, obtained from a fish database (http://fishbase.org), and plotted the body-brain mass relationships. The fish and environments examined included the following: 1) 35 fish species living in polar conditions in which the average temperature was ~1°C, 2) 70 fish species living in temperate conditions with an average water temperature of ~15°C, 3) 88 species living in tropical conditions with an average water temperature of 25°C, and 4) 17 shark species living in sub-tropical waters that maintain their body temperatures at 20-30°C via steady contractile activity of muscles , (Additional file 2: Table S2; these data were randomly selected from the fish database). Surprisingly, we found that the fish brain-to-body mass ratio data fell into 3 distinct groups (Figure 1B). Fish living in warm tropical water had brains that were 2–5 times larger than those of the temperate-water fish and 3–15 times larger than those of the fish living in cold polar water. The fit equation for the fish species living in tropical conditions had an allometric slope of 0.7 and a constant of 0.0118 (red line and symbols), the equation for fish living in the temperate waters had an allometric slope of 0.64 and a constant of 0.0078 constant (green line and symbols), and the fish living in the polar waters had the shallowest allometric slope (0.59) and a constant of 0.0036.
Figure 1C directly quantifies the allometric relationship between the environmental temperature (from 1°C to 25°C) of the fish and their average brain mass for two body masses (10 g and 1,000 g). The fit equations indicate that fish brain mass more than doubles for each 10°C increase in temperature. As a comparison, the average mammalian brain sizes in animals weighing 10 g and 1,000 g are plotted in this figure (with a constant temperature of 38°C). This figure shows a nice fit between body mass and brain temperature for three points, but not for the largest brain sizes and highest temperatures. The data from the larger brain sizes/higher temperatures falls above the fit lines, indicating that temperature alone may not be the sole contributor to the development of larger brains. Other factors may contribute to the development of larger brains in mammals.
Together, these data indicate that temperature may be a critical factor in the marked changes in brain size during evolution in different climate conditions. Specifically, for a given body mass, warmer living conditions should result in larger brains.
In addition to this temperature-driven brain enlargement, the difference in brain sizes of endotherms and ectotherms increases as body size increases (Figure 1D). For small body sizes (below 1 gram), the brains of endotherms are atleast 4 times larger than those of ectotherms (black line in Figure 1D). Similarly, tropical-water fish have brains that are at least 3 times larger than those of cold-water fish (dashed line). In contrast to these effects in animals with small body sizes, for animals with body sizes over 100 kilograms, the brains of endotherms are approximately 20 to 40 times larger than those of ectotherms, and tropical-water fish have brains that are more than 10 to 15 times larger than those of cold-water fish. In comparison, the brains of primates are, relatively consistently, 2–3 times larger than those of other mammals, and this difference is less dependent on body size differences (dotted line in Figure 1D).
The emergence of intrinsically constant warm body temperatures together with an increasing brain size, with increasing body mass may be related to a temperature-dependent regulation of body metabolism. It is possible, that changes in body or environmental temperature affects the metabolic rate and consequently affected the evolution of large brains. This effect may depend on body mass.
The basal metabolic rate of the body (Mbody) has been found to be governed primarily by two interacting processes : the Boltzmann-Arrhenius factor, which describes the temperature dependence of biochemical processes , and an allometric relation that describes how biological rate processes scale with body size for all vertebrates ,,-.
where P is body weight, C1 is a constant (whose value is different for different species), γ is the allometric scaling factor, T is the absolute temperature, Ev is the activation energy (defined as the minimum amount of energy required to initiate a particular process. It is usually used in the context of chemical reactions, i.e., as the minimum amount of energy that chemical reactants must possess before they can undergo a chemical reaction. For the life metabolic process regulated by the temperature, E v is expected to be around 0.65 electron Volts (eV) ), and k is Boltzmann’s constant (8.6×10−5 eV/K, here K is the temperature in Kelvin units). Earlier analyses have revealed that C1 = 0.0305 and γ = 0.682 for endotherms (these data, which are primarily from mammals and birds, were taken from references ,), measured and scaled at 38°C, and C1 = 0.000525 and γ = 0.81 for ectotherms when normalized to a temperature of 16°C (see Figure 2A and Additional file 3: Table S3); these findings are consistent with those of recent reports ,,. When the metabolic rates of the endotherms are normalized to a common temperature of 16°C using Eq. (2), the metabolic rates of the endotherms (e.g., mammals and birds) remain several times higher than those of ectotherms (e.g., fish, amphibians and reptiles), particularly for animals with small body sizes (Figure 2B). In large animals, the metabolic rates of the endotherms and ectotherms were similar (Figure 2B and C). These findings suggest that at similar metabolic rates, large endotherms should support brain masses several times greater than those of ectotherms (Figure 2D), given that brain size differences increase with body size among endotherms and ectotherms (Figure 1D).
These analyses suggest that temperature-dependent body metabolism alone cannot explain the differences in brain size among endotherms than among ectotherms. These results also indicate that other factors may play a role in explaining the differences in brain size between warm blooded endothermic animals and cold blooded exothermic animals. It is possible that neural, glial density or the energy demands on these cells are dependent on body temperature.
A general model linking brain and body sizes to metabolism and temperature
In this section, we derive a general model to quantify the relationships between brain size, temperature-dependent metabolism, individual neuronal metabolism and body size to account for the origin of Eq. (1). We began by considering the relationship between brain mass and the number of neurons and supporting cells in the brain. Brain mass (W) is determined by the total number of neurons and supporting cells; hence W = Nw+ N 1 w 1 , where N and w are the number and weight of brain neurons, respectively, and N1 and w1 are the corresponding quantities for the supporting cells. If we assume that the weight and number of the supporting cells are proportional to those of the neurons, i.e., if N 1 = k 1 N, and w 1 = k 2 w, then the relationship between brain mass and neurons simplifies to W = C 2 N, where C 2 = (1+k 1 k 2 )w. This theoretical picture in which W is proportional to N is supported by a recent analysis of mammalian and primate brains (see also Figure 3A and Additional file 4: Table S4). Analyses of these data yielded.
where β is close to 1 (here C2 = 0.0052 and β = 1.12 for both rodents and primates) as reported in recent studies ,-, . The numbers of supporting non-neuronal cells in endotherms are proportional to the numbers of neurons and are also strongly correlated with brain mass (Figure 3B). The supporting cells play an important role in providing mechanical and trophic support to neurons. Additionally, data from several ectothermic animals ,- indicate that that C2 = 0.0096 and β = 0.83 (see Additional file 4: Table S4; the numbers of non-neuronal cells are not available for ectotherms).
Neurons are the brain’s major energy consumer, and most (~80%) of the brain’s energy is consumed by signaling processes within neurons ,. Brains are energetically costly. Experimental investigations have shown that the brain’s metabolic consumption accounts for 1-10% of the total body metabolic rate (Mbody) in most animal species, although brain mass accounts for no more than 1% of an animal’s body weight ,. Hence, the total energy required to support the brain can be quantified as
where Mbrain is the brain’s basal metabolic rate, Mbody is the body’s basal metabolic rate as defined in Eq. (2), and φ is a variable that varies with species. Re-examination of the data reported by Mink et al. (1981) suggested that φ can be described by the following equation for both ectotherms and endotherms
The total amount of energy supplied to the brain (Mbrain) should be equal to the total energy consumption of the brain’s neurons. Note that here we make a simplifying assumption, by considering the metabolic costs of neurons only because neurons consume most of the energy while the supporting cells account for 10-20% of total brain energy. If the total number of neurons in the brain is N and the metabolic consumption of each individual neuron is Mneuron, we can describe the relationship as follows:
where the total number of neurons in the brain (N) contributes directly to the brain’s mass (see Eq. 2). By inserting Eqs. (2)-(5) into Eq. (6), we obtained a general model of the relationships between brain mass (W), body mass (P) and temperature (T):
where , α = βγ + βξ, and , called effective temperature. Equation (7) links body temperature, body mass, brain mass and neuron metabolic rate (Mneuron), provides a general theoretical framework for quantifying changes in brain size during evolution, and provides an origin of the formula of Eq. (1) that was derived from experimental data. The temperature range used for Eq. (7) is 0 ~ 42°C, corresponds to what is normally observed in polar fish to the body temperature of birds. The equations are not valid for temperatures above or below these values, because all the scaling parameters have been derived from animal data acquired within this temperature range.
Equation (7) links brain mass with the metabolic consumption rate of individual neurons (Mneuron). Recent studies suggest that the energy cost per neuron (Mneuron) in the brain is stable and nearly invariant across mammal species . Calculations based on the available data revealed that Mneuron is, on average, 2.51 × 10−10 watts for endotherms and 0.563 × 10−10 watts for ectotherms (see Figure 4C and Additional file 5: Table S5).
We inserted empirically determined parameters (see the analyses in Figures 3 and 4) into Eq. (7) to determine how well this data-based theoretical model predicts actual animal brain size. Figure 5A plots the results of linerar regression analysis between log10 (brain mass) and log10 (brain mass prediction) for endotherms (red line) -- mammals, birds, primates and dolphins, primarily -- at 38°C and endothermic animals at 16°C (black line). The predicted parameters: for endotherms were λ = 0.0749 and α = 0.6608, and the analysis results were a correlation coefficient CC = 0.979, an adjusted-R2 = 0.958, and a mean squared error MSE = 0.042, p-value p <1×10−5). For endothermic animals at 16°C, the predicted parameters were: λ = 0.0085 and α = 0.5876) and the multiple linear regression analysis results were CC = 0.966, adjusted-R2 = 0.933, and a MSE = 0.0552 (p-value <1×10−5). In this analysis body mass P was the regressor variable and temperature was a constant. The model predictions closely matched the real brain data, with data-derived parameters of C1 = 0.0305, γ = 0.682, C2 = 0.0052, β = 1.12, C3 = 0.081, and ζ = −0.092 for the endotherm-based data analysis that resulted in the equation (where P represents body mass in gram) based on Eq. (7) and C1 = 0.000525, γ = 0.81, C2 = 0.0096, β = 0.83, C3 = 0.092 and ζ = −0.102 for ectotherms, which led to the following equation: . The difference in brain size of endotherms compared to ectotherm (i.e., Brainendotherm/Brainectotherm) also increased from 7.4 to 36-fold with increasing body masses (inset of Figure 5A). If we constrain all parameters in ectotherms, in the same fashion as we have done for endotherms with the exception of the temperature difference, the brain size prediction for the ectotherms is only 7.4-fold lower than that for the endotherms (blue line in Figure 5A and, the inset). Furthermore the scaling parameter of α = 0.6608 from Eq. (7) is close to the measured value of 0.689 for endotherms, and α = 0.5876 is close to the measured value of 0.578 for ectotherms (Figure 1). Thus, temperature-regulated metabolism can only partially explain the 4-40-fold brain size difference shown in Figure 1A, and other factors, such as increases in non-neuron cell (mostly glial) numbers (as reflected in Figure 4B), may be essential factors in explaining brain size differences. Thus, contributions from metabolism, neural density and temperature together explain most of the differences in brain mass between endotherms and ectotherms shown in Figure 1A.
Note that model predictions for endothermic brain sizes are generally 2–5 times lower than actual brain masses of primates and dolphins, suggesting there other evolutionary factors mat contribute to the larger brain size in these species, as has been indicated in recent reports ,,. The model in the present study does not make any attempt to capture these effects.
The model predictions for tropical-water fish, temperate-water fish, and cold-water fish are shown in Figure 5B, which predicts that brain size at 25°C should be 6-fold higher than that in fish at 1°C and that at 15°C brain size should be 3-fold higher than at 1°C. The model used actual data-derived parameters of C1 = 0.00051, γ = 0.879, C2 = 0.0096, β = 0.83, C3 = 0.092 and ζ = −0.102, which for fish results in the equation based on Eq. (7). Note, data for fish brain, glial density data are currently not available, so the fitting is imperfect, and only includes the temperature- related differences.
The model prediction for insectivores (with body temperatures of approximately 31°C) is perfectly aligned with the actual data with predicted parameters of λ = 0.0409 and α = 0.6608 (CC = 0.952, adjusted- R2 = 0.91, MSE = 0.0211, p < 1×10−5). Figure 5D quantifies the allometric relationship between the temperatures that fish live at, and their average brain mass for two body masses (10 g and 1,000 g). The equations for the fish were given by and for mammals were given by . At a given body weight, as temperature increases, brain mass of fish increases, but the increase of brain mass with temperature is larger in mammals. This relationship is well captured by the model predictions (see the black line in Figure 5D), with multiple linear regression analysis -- here temperature T is the regressor variable -- between log10 (actual brain mass) and log10(model prediction) for 10 g: CC = 0.998, adjusted- R2 = 0.995, MSE = 0.0041, p < 0.0023; and for 1000 g: CC = 0.9999, adjusted- R2 = 0.9998, MSE = 0.0049, p < 0.0001.
By applying the general model described by Eq. (7) to all the major types of vertebrates (including endotherms: primates, dolphins, mammals and birds, and ectotherms: fishes, reptiles and amphibians), we found that the actual brain masses and model predictions were aligned well in a log-log plot (Figure 6A). The multiple linear regression results were CC = 0.979, adjusted- R2 = 0.958, MSE = 0.0421, p < 10−5 for endotherms and CC = 0.966, adjusted- R2 = 0.933, MSE = 0.0522, p < 10−5 for ectotherms. Figure 6B shows the residuals of the log brain sizes i.e., log10(actual brain mass)-log10(model prediction), for both the endotherms and ectotherms as a function of log body size with slopes close to 0, indicating that the model performed well in predicting actual brain mass. Figure 6C shows that actual brain masses of fishes and model predictions were also aligned in a log-log plot (CC = 0.966, adjusted- R2 = 0.932, MSE = 0.0674; p < 1×10−5). The model predictions were underestimates compared to the actual brain masses for the tropical fish with larger body sizes, and were overestimates compared to the actual brain masses for smaller tropical fish. The model underestimated the actual brain masses for fish from cold – i.e. polar -- water (Figure 6C), but for the temperate-water fish the model matched the real data well (Figure 6C). Note that the model is missing parameters, it is worth noting that the model relies on actual data, and that for both tropical-water and polar-water fish there is a dearth of data for neural and supporting cell densities. With adequate samples of all important measures, i.e. the kinds of data we have for mammals and primates, shown in Figure 3, the model predictions would be improved. Figure 6D shows the residuals of the log brain sizes, i.e., log10(actual brain mass)-log10(model prediction), for three groups of fishes as a function of log body sizes. This has a flat relationship with slopes close to 0, indicating that the model performed well in predicting actual fish brain mass.
Finally, we used Eq. (7) to predict brain sizes of all the types of vertebrates (also including 7 mesozoic and 18 archiac mammals (with body temperature around 31°C) and 17 dinosaurs (with temperature 16°C)). Top panel in Figure 7A shows that actual brain masses, and the model predictions tracked each other well in a log-log plot (multiple linear regression analysis results: CC = 0.978, adjusted- R2= 0.956; MSE = 0.0495; p < 1 × 10−5). Compared to the ten- to hundred-fold variance in brain size for the same body mass shown in Figure 1A, the model predictions and actual brain sizes exhibited a rather small variance, and only ~ 5-fold difference (Figure 7A). The predicted brain mass from the fitting equations in Figure 1, and the actual brain mass also tracked each other well, but the variance was a little bit higher, roughly 8 fold than in the analysis used in top panel. The multiple linear regression analysis of these data show that: CC = 0.973, adjusted- R2 = 0.946; MSE = 0.0544; p < 1 × 10−5. Note that the adjusted- R2 = 0.946 is slightly less than the value 0.956 in top panel using Eq. (7). This suggests that Eq. (7) fits the data better than do the fitting equations from Figure 1A because that both body mass and temperature play important roles in determining the brain mass.
For most of animal species (i.e., temperate and tropic fishes, reptiles, amphibians, insectivores, mammals and birds), model predictions matched real brain data well, with CC > 0.95, MSE = 0.07, p < 10−5) with ratios of (model prediction)/(actual brain mass) close to 1 (Figure 7B) while there are larger mean square errors for primates (MSE = 0.23, CC = 0.956) and dolphins (MSE = 0.544, CC = 0.97), and dinosaurs (MSE = 0.51, CC = 0.803) with ratios of (model prediction)/(actual brain mass) either much less or much more than 1 (Figure 7B). The regression analysis gives CC = 0.965, adjusted- R2 = 0.932, MSE = 0.089, p < 1 × 10−5 for archiac mammals, and CC = 0.97, adjusted- R2 = 0.94, MSE = 0.0162, p < 1 × 10−3 for mesozoic mammals.
The predictions of the model could be improved by more accurate measurements of the body temperature, body metabolism, neuronal metabolism and neuronal/glial densities of each group of species.
Discussion and conclusion
There is increased interest in examining the key factors facilitating enlargement of the brain during evolution. Given that the energy cost of the brain’s neurons is more than ten times that of the cells of the body, the factors that regulate the brain’s energy supply and energy cost should be given thorough consideration. In this study, we re-examined data on brain size, body size and metabolic rates for all the major types of vertebrates, developed a theoretical framework based on examination of the real data, and identified several critical factors that may trigger the dramatic enlargement of the brains of birds and mammals as compared with fish, reptiles and amphibians. The most critical factor was temperature. When the endothermic animals evolved from ectothermic animals, two unique features were bound together in endotherms: larger brains and higher body temperatures (on average, 20°C higher than those of ectothermic animals). The warm temperature was critical in speeding the biochemical processes and making marked metabolic differences. Our analysis revealed that in small animals the basal metabolic rate of endotherms is 60 times greater than that of ectotherms, and in large animals it is ~ 10 times greate (Figure 2C). However, the brain sizes of small endotherms are only approximately 4 times greater than that of small ectotherms but the brains of large endotherms are more than 40 times greater than the brains of large ectotherms. Clearly, in large animals, the same basal metabolic rate of endothermic animals should support larger brain tissue in ectothermic animals, than it actually does (Figure 2D), suggesting that temperature was only one of the key factors in mediating the enlargement of the brains of endotherms. Note a similar analysis published recently points out that temperature correlates with brain size .
Other factors included in our analysis are brain metabolism and number of supporting glial neurons. Analysis of the brain metabolism data showed that that the average metabolic cost of individual neurons in ectothermic brains is ~ 5.63 × 10−11 watts, which is nearly 5 times lower than the average metabolic cost of endothermic brains (Figure 4C). The lower energy cost of individual neurons could be attributed to the effect of colder temperature on metabolism and the occurrence of fewer synapses in ectothermic brains , or the lower energy cost of individual neurons could be associated to the smaller number of glia in ectotherms ,,. Glia are the major non-neuronal supporting cells that maintain homeostasis, form myelin, and provide mechanical and tropic support to neurons. In the vertebrate CNS, most glia originate from portions of the developing neural tube—the exception being olfactory ensheathing glia —and the energy cost of glia is generally only a few percent of that of neurons ,. Recent data suggest that the evolution of the nervous system was accompanied by increases in the number and size of glia ,,. In vertebrates, the glia-to-neuron ratio in the cortex increases with the size of the brain ,. Figure 3 and Eq. (3) show that brain mass increases much faster in endothermic brains than in ectothermic brains as the number of neurons increase, which is also suggestive of increases in the numbers of non-neuronal supporting cells. Glia contribute to the faster propagation of nerve signals and long-range communications, particularly via the evolution of myelinating glia in developed endotherms . These energetically inexpensive glial cells surround neurons and occupy a relatively large portion of the brain , while also providing energy support to neurons and aiding efficient communication between cortical areas ,. Thus, these cells may be one of the critical factors that promoted brain enlargement.
The evolution of the large brains of endotherms is associated with multiple factors. To quantify the contribution of each factor to the brains of mammals and birds that are tens of times larger than those of fish, reptiles and amphibians, we built up a general theoretical model to examine the origin of body-brain size allometric scaling relationship by linking the key factors of temperature-dependent metabolism, neuron energetics, neuronal density and body size. This basic framework suggests that temperature and the development of supporting non-neuronal cells may have been the critical factors that correlated with, or might even be causal, for the enlargement of the brains of endotherms (Figure 5A). This framework also predicts that the brains of cold-blooded animals living in warmer conditions should be several times larger than those of cold-blooded animals that live in colder conditions. This prediction was confirmed by the analysis of the brain sizes of fish living in various temperatures (Figures 1B and 5B). Indeed, owing to the absence of measurements of neuronal density (and glia-to-neuron ratios) for the brains of fish that live in different temperatures, the model prediction in Figure 5B did not capture the differences in the slopes between the tropical and polar fish that are shown in Figure 1B and D. However, a prediction from the endothermic brain would be that tropical fishes have larger numbers of glia in their brains than do the fish that live in cold conditions. These predictions can be tested by experimental investigations.
No new animal/human data was generated in this study. All the data used in this study are from references and online database.
Animal data sources
The brain and body size datasets for the endothermic and ectothermic animals (Figure 1A) are from references ,,, and are listed in Additional file 1: Table S1. The body and brain size datasets for fish that live in different temperatures and were used to create Figure 1B were collected from the database at www.fishbase.org and are listed in Additional file 2: Table S2. The metabolism-to-body scaling relationships of the endothermic and ectothermic animals were collected from published papers ,- that are listed in Additional file 3: Table S3 and are plotted in Figure 2. The data regarding the allometric scaling of the brain mass and total neuronal numbers for the endothermic and ectothermic animals were collected from published papers , and are listed in Additional file 4: Table S4 and plotted in Figure 3. The datasets for the ratios of brain metabolism to resting body metabolism are listed in Additional file 5: Table S5, are plotted in Figure 4 and were collected from published papers .
YY conducted research, analysed the data, built up the mathematical model, worte the paper. JK helped mathematical model corrections, RS helped write the paper and result discussion, JF helped data statistical method. All authors reviewed the manuscript.
Roth G, Dicke U: Evolution of the brain and intelligence. Trends Cogn Sci. 2005, 9: 250-257. 10.1016/j.tics.2005.03.005. doi:10.1016/j.tics.2005.03.005
Jarvis ED, Güntürkün O, Bruce L, Csillag A, Karten H, Kuenzel W, Medina L, Paxinos G, Perkel DJ, Shimizu T, Striedter G, Wild JM, Ball GF, Dugas-Ford J, Durand SE, Hough GE, Husband S, Kubikova L, Lee DW, Mello CV, Powers A, Siang C, Smulders TV, Wada K, White SA, Yamamoto K, Yu J, Reiner A, Butler AB, Avian Brain Nomenclature Consortium: Avian brains and a new understanding of vertebrate brain evolution.Nat Rev Neurosci6:151–159. doi:10.1038/nrn1606 (2005).,
Striedter GF: Principles of Brain Evolution. Sinauer Associates; 2005.
Isler K, van Schaik CP: Metabolic costs of brain size evolution. Biol Lett. 2006, 2: 557-560. 10.1098/rsbl.2006.0538. doi:10.1098/rsbl.2006.0538
Connor RC: Dolphin social intelligence: complex alliance relationships in bottlenose dolphins and a consideration of selective environments for extreme brain size evolution in mammals. Philos Trans R Soc Lond B Biol Sci. 2007, 362: 587-602. 10.1098/rstb.2006.1997. doi:10.1098/rstb.2006.1997
Dunbar RI, Shultz S: Evolution in the social brain.Science317:1344–1347. doi:10.1126/science.1145463 (2007).,
Niven JE, Laughlin SB: Energy limitation as a selective pressure on the evolution of sensory systems. J Exp Biol. 2008, 211: 1792-1804. 10.1242/jeb.017574. doi:10.1242/jeb.017574
Dunbar RI: The social brain hypothesis and its implications for social evolution.Ann Hum Biol36:562–572. doi:10.1080/03014460902960289912879712 (2009).,
Isler K, van Schaik CP: The Expensive Brain: a framework for explaining evolutionary changes in brain size. J Hum Evol. 2009, 57: 392-400. 10.1016/j.jhevol.2009.04.009. doi:10.1016/j.jhevol.2009.04.009
Sol D, Garcia N, Iwaniuk A, Davis K, Meade A, Boyle WA, Székely T: Evolutionary Divergence in Brain Size between Migratory and Resident Birds. PLoS One. 2010, 5: e9617-10.1371/journal.pone.0009617. doi:10.1371/journal.pone.0009617
Amiel JJ, Tingley R, Shine R: Smart moves: effects of relative brain size on establishment success of invasive amphibians and reptiles. PLoS One. 2011, 6: e18277-10.1371/journal.pone.0018277. doi:10.1371/journal.pone.0018277
Navarrete A, van Schaik CP, Isler K: Energetics and the evolution of human brain size. Nature. 2011, 480: 91-93. 10.1038/nature10629. doi:10.1038/nature10629
Matsuzawa T: Evolution of the brain and social behavior in chimpanzees.Curr Opin Neurobiol23:443–449. doi:10.1016/j.conb.2013.01.012S0959-4388(13)00028-7 (2013).,
Ruben J: The Evolution of Endothermy in Mammals and Birds: From Physiology to Fossils.Annu Rev Physiol57:69–95. doi:10.1146/annurev.ph.57.030195.000441 (1995).,
Marshall LG, Butler RF, Drake RE, Curtis GH: Calibration of the beginning of the age of mammals in patagonia. Science. 1981, 212: 43-45. 10.1126/science.212.4490.43. doi:10.1126/science.212.4490.43
Jerison HJ: Evolution of the Brain and Intelligence Academic Press; 1973.
Kaas JH: The Evolution of Brains from Early Mammals to Humans.Wiley Interdiscip Rev Cogn Sci4:33–45. doi:10.1002/wcs.1206 (2013).,
Snell O: Hexenprozesse und Geistesstorung. 1891, Psychiatrische Untersuchungen, Munchen, Lehman
Martin RD: Relative brain size and basal metabolic rate in terrestrial vertebrates. Nature. 1981, 293: 57-60. 10.1038/293057a0.
West GB, Brown JH, Enquist BJ: A general model for the origin of allometric scaling laws in biology. Science. 1997, 276: 122-126. 10.1126/science.276.5309.122.
Barrickman NL, Lin MJ: Encephalization, expensive tissues, and energetics: An examination of the relative costs of brain size in strepsirrhines. Am J Phys Anthropol. 2010, 143: 579-590. 10.1002/ajpa.21354. doi:10.1002/ajpa.21354
Weisbecker V, Goswami A: Brain Size, Life History, and Metabolism at the Marsupial/Placental Dichotomy Proceedings of the National Academy of Sciences; 2010. doi:10.1073/pnas.0906486107.
Herculano-Houzel S: Scaling of brain metabolism with a fixed energy budget per neuron: implications for neuronal activity, plasticity and evolution. PLoS One. 2011, 6: e17514-10.1371/journal.pone.0017514. doi:10.1371/journal.pone.0017514
Hofman MA: Energy Metabolism, Brain Size and Longevity in Mammals. Q Rev Biol. 1983, 58: 495-512. 10.1086/413544.
Mink JW, Blumenschine RJ, Adams DB: Ratio of central nervous system to body metabolism in vertebrates: its constancy and functional basis. Am J Physiol. 1981, 241: R203-R212.
Anderson SD, Goldman KJ: Temperature Measurements from Salmon Sharks, Lamna ditropis, in Alaskan Waters. Copeia. 2001, 2001: 794-796. 10.1643/0045-8511(2001)001[0794:TMFSSL]2.0.CO;2.
Bernal D, Donley JM, Shadwick RE, Syme DA: Mammal-like muscles power swimming in a cold-water shark.Nature437:1349–1352. (2005)., [http://www.nature.com/nature/journal/v437/n7063/suppinfo/nature04007_S1.html]
Gillooly JF, Brown JH, West GB, Savage VM, Charnov EL: Effects of size and temperature on metabolic rate. Science. 2001, 293: 2248-2251. 10.1126/science.1061967. doi:10.1126/science.1061967
Kondepudi DK, Prigogine I: Modern Thermodynamics: From Heat Engines to Dissipative Structures. John Wiley & Sons; 1998.
Schmidt-Nielsen K: Scaling, Why is Animal Size so Important? Cambridge University Press; 1984.
Seymour RS: Biophysics and physiology of temperature regulation in thermogenic flowers. Biosci Rep. 2001, 21: 223-236. 10.1023/A:1013608627084.
White CR, Seymour RS: Mammalian basal metabolic rate is proportional to body mass2/3. Proc Natl Acad Sci U S A. 2003, 100: 4046-4049. 10.1073/pnas.0436428100. doi:10.1073/pnas.0436428100
Brown JH, Gillooly JF, Allen AP, Savage VM, West GB: Toward a metabolic theory of ecology. Ecology. 2004, 85: 1771-1789. 10.1890/03-9000. doi:10.1890/03-9000
Glazier DS: Beyond the `3/4-power law’: variation in the intra- and interspecific scaling of metabolic rate in animals. Biol Rev Camb Philos Soc. 2005, 80: 611-662. 10.1017/S1464793105006834. doi:10.1017/S1464793105006834
White CR, Seymour RS: Allometric scaling of mammalian metabolism. J Exp Biol. 2005, 208: 1611-1619. 10.1242/jeb.01501. doi:10.1242/jeb.01501
Clarke A: Temperature and the metabolic theory of ecology. Functional Ecology. 2006, 20: 405-412. 10.1111/j.1365-2435.2006.01109.x. doi:10.1111/j.1365-2435.2006.01109.x
White CR, Phillips NF, Seymour RS: The scaling and temperature dependence of vertebrate metabolism. Biol Lett. 2006, 2: 125-127. 10.1098/rsbl.2005.0378. doi:10.1098/rsbl.2005.0378
Karbowski J: Global and regional brain metabolic scaling and its functional consequences. BMC Biol. 2007, 5: 18-10.1186/1741-7007-5-18. doi:10.1186/1741-7007-5-18
Savage VM, West GB: A quantitative, theoretical framework for understanding mammalian sleep. Proc Natl Acad Sci U S A. 2007, 104: 1051-1056. 10.1073/pnas.0610080104. doi:10.1073/pnas.0610080104
White CR, Cassey P, Blackburn TM: Allometric exponents do not support a universal metabolic allometry. Ecology. 2007, 88: 315-323. 10.1890/05-1883.
Glazier DS: A unifying explanation for diverse metabolic scaling in animals and plants. Biol Rev Camb Philos Soc. 2010, 85: 111-138. 10.1111/j.1469-185X.2009.00095.x. doi:10.1111/j.1469-185X.2009.00095.x
Makarieva AM, Gorshkov VG, Li BL, Chown SL, Reich PB, Gavrilov VM: Mean mass-specific metabolic rates are strikingly similar across life’s major domains: Evidence for life’s metabolic optimum. Proc Natl Acad Sci U S A. 2008, 105: 16994-16999. 10.1073/pnas.0802148105. doi:10.1073/pnas.0802148105
Clarke A, Rothery P, Isaac NJ: Scaling of basal metabolic rate with body mass and temperature in mammals.J Anim Ecol79:610–619. doi:10.1111/j.1365-2656.2010.01672.x (2010).,
Froese RADPE: FishBase. World Wide Web Electronic Publication; 2012. , version., [www.fishbase.org]
Tower DB: Structural and functional organization of mammalian cerebral cortex; the correlation of neurone density with brain size; cortical neurone density in the fin whale (Balaenoptera physalus L.) with a note on the cortical neurone density in the Indian elephant. J Comp Neurol. 1954, 101: 19-51. 10.1002/cne.901010103.
Herculano-Houzel S, Collins CE, Wong P, Kaas JH: Cellular scaling rules for primate brains. Proc Natl Acad Sci U S A. 2007, 104: 3562-3567. 10.1073/pnas.0611396104. doi:10.1073/pnas.0611396104
Herculano-Houzel S, Mota B, Lent R: Cellular scaling rules for rodent brains. Proc Natl Acad Sci U S A. 2006, 103: 12138-12143. 10.1073/pnas.0604911103. doi:10.1073/pnas.0604911103
Kemali M, Braitenberg V: Atlas of the Frog’s Brain. Springer; 1969.
Packard A, Albergoni V: Relative growth, nucleic acid content and cell numbers of the brain in Octopus vulgaris (Lamarck). J Exp Biol. 1970, 52: 539-552.
Zupanc GK, Horschke I: Proliferation zones in the brain of adult gymnotiform fish: a quantitative mapping study. J Comp Neurol. 1995, 353: 213-233. 10.1002/cne.903530205. doi:10.1002/cne.903530205
Howarth C, Gleeson P, Attwell D: Updated energy budgets for neural computation in the neocortex and cerebellum. J Cereb Blood Flow Metab. 2012, 32: 1222-1232. 10.1038/jcbfm.2012.35. doi:10.1038/jcbfm.2012.35
Hyder F, Rothman DL, Bennett MR: Cortical energy demands of signaling and nonsignaling components in brain are conserved across mammalian species and activity levels. In Proceedings of the National Academy of Sciences of the United States of America. doi:10.1073/pnas.1214912110 (2013).
Roth G, Dicke U: Evolution of the brain and intelligence in primates.195:413–430. doi:10.1016/B978-0-444-53860-4.00020-9 (2012).,
Gillooly JF, McCoy MW: Brain size varies with temperature in vertebrates.Peer J2:e301. doi:10.7717/peerj.301301 (2014).,
Braitenberg V, Schüz A: Cortex: Statistics and Geometry of Neuronal Connectivity. Springer; 1998.
Hartline DK: The evolutionary origins of glia. Glia. 2011, 59: 1215-1236. 10.1002/glia.21149. doi:10.1002/glia.21149
Ramon-Cueto A, Avila J: Olfactory ensheathing glia: properties and function.Brain Res Bull46:175–187 (1998).,
Oberheim NA, Wang X, Goldman S, Nedergaard M: Astrocytic complexity distinguishes the human brain.Trends Neurosci29:547–553. doi:10.1016/j.tins.2006.08.004 (2006).,
Hartline DK, Colman DR: Rapid conduction and the evolution of giant axons and myelinated fibers.Curr Biol17:R29–35. doi:10.1016/j.cub.2006.11.042 (2007).,
Azevedo FA, Carvalho LR, Grinberg LT, Farfel JM, Ferretti RE, Leite RE, Jacob Filho W, Lent R, Herculano-Houzel S: Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain. J Comp Neurol. 2009, 513: 532-541. 10.1002/cne.21974. doi:10.1002/cne.21974
Boddy AM, McGowen MR, Sherwood CC, Grossman LI, Goodman M, Wildman DE: Comparative analysis of encephalization in mammals reveals relaxed constraints on anthropoid primate and cetacean brain scaling. J Evol Biol. 2012, 25: 981-994. 10.1111/j.1420-9101.2012.02491.x. doi:10.1111/j.1420-9101.2012.02491.x
Sarko DK, Catania KC, Leitch DB, Kaas JH, Herculano-Houzel S: Cellular scaling rules of insectivore brains. Front Neuro. 2009, 3: 8-doi:10.3389/neuro.05.008.2009
Gabi M, Collins CE, Wong P, Torres LB, Kaas JH, Herculano-Houzel S: Cellular scaling rules for the brains of an extended number of primate species. Brain Behav Evol. 2010, 76: 32-44. 10.1159/000319872. doi:10.1159/000319872
We thank Harry J. Jerison for sharing the brain-body mass data. We express our deep gratitude to Fahmeed Hyder, Gordon M Shepherd, Michael Hines, and David Waxman for valuable discussions and comments. This project was funded by the National Natural Science Foundation of China (31271170) and was also supported by the program for the Professor of Special Appointment (Eastern Scholar SHH1140004) at Shanghai Institutions of Higher Learning.
The authors declare that they have no competing interests.